##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938249_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 438624 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.84323931203035 33.0 31.0 34.0 30.0 34.0 2 31.995583916976727 34.0 31.0 34.0 30.0 34.0 3 32.01948365798497 34.0 31.0 34.0 30.0 34.0 4 35.520103779090974 37.0 35.0 37.0 35.0 37.0 5 35.57704776756402 37.0 35.0 37.0 35.0 37.0 6 35.664138305245494 37.0 37.0 37.0 35.0 37.0 7 35.6242886846137 37.0 36.0 37.0 35.0 37.0 8 35.61877143065587 37.0 37.0 37.0 35.0 37.0 9 37.26850559932881 39.0 38.0 39.0 35.0 39.0 10-11 37.23053230101408 39.0 38.0 39.0 35.0 39.0 12-13 37.15410123477056 39.0 38.0 39.0 35.0 39.0 14-15 38.33304037170789 40.0 38.0 41.0 34.0 41.0 16-17 38.29243383854965 40.0 38.0 41.0 34.5 41.0 18-19 38.32667501094332 40.0 38.0 41.0 34.5 41.0 20-21 38.28292683482891 40.0 38.0 41.0 34.5 41.0 22-23 38.12993817027796 40.0 38.0 41.0 34.0 41.0 24-25 38.027308127234264 40.0 38.0 41.0 34.0 41.0 26-27 37.91373363062669 40.0 38.0 41.0 34.0 41.0 28-29 37.77337309403954 40.0 37.0 41.0 34.0 41.0 30-31 37.450278598526296 40.0 36.5 41.0 33.5 41.0 32-33 37.04676556686365 40.0 35.0 41.0 33.0 41.0 34-35 36.775220918144015 39.5 35.0 41.0 33.0 41.0 36-37 36.50141009155906 39.0 35.0 41.0 32.5 41.0 38-39 36.21773318377471 39.0 35.0 41.0 31.0 41.0 40-41 35.9738865178376 39.0 35.0 41.0 31.0 41.0 42-43 35.60442999015102 39.0 35.0 41.0 30.0 41.0 44-45 35.1736065513971 39.0 35.0 41.0 26.5 41.0 46-47 34.742787672357196 39.0 35.0 41.0 23.0 41.0 48-49 34.35272123732399 39.0 35.0 41.0 17.5 41.0 50-51 33.676731095425694 38.5 34.0 40.5 10.0 40.5 52-53 33.742574505727 38.5 35.0 40.0 4.5 41.0 54-55 33.86237757167871 39.0 35.0 41.0 2.0 41.0 56-57 33.667241190632524 39.0 35.0 41.0 2.0 41.0 58-59 33.442566982198876 38.5 35.0 41.0 2.0 41.0 60-61 33.0970307142336 38.0 34.5 40.0 2.0 41.0 62-63 32.73320885314073 38.0 34.0 40.0 2.0 41.0 64-65 32.37794785511053 37.5 33.5 40.0 2.0 41.0 66-67 31.963312541037425 37.0 33.0 40.0 2.0 41.0 68-69 31.49807124097177 36.0 32.5 39.0 2.0 41.0 70-71 31.032405887502733 35.5 32.0 39.0 2.0 40.5 72-73 30.54248855511782 35.0 31.0 38.5 2.0 40.0 74-75 30.03287211826074 35.0 31.0 37.5 2.0 39.5 76-77 29.54406279638141 35.0 30.5 37.0 2.0 39.0 78-79 28.98763063580652 35.0 29.0 36.0 2.0 39.0 80-81 28.45621306631648 35.0 29.0 36.0 2.0 37.5 82-83 27.934256903407018 34.0 27.5 35.0 2.0 37.0 84-85 27.44872259611877 34.0 26.5 35.0 2.0 37.0 86-87 27.026570593492377 34.0 26.0 35.0 2.0 36.0 88-89 26.49391392171883 34.0 24.5 35.0 2.0 36.0 90-91 25.97192470088276 33.5 22.0 35.0 2.0 35.5 92-93 25.443130106879696 33.0 19.0 35.0 2.0 35.0 94-95 24.966173761581672 33.0 11.0 35.0 2.0 35.0 96-97 24.299803476325962 32.5 2.0 35.0 2.0 35.0 98-99 23.48887999744656 32.0 2.0 35.0 2.0 35.0 100-101 21.020976964324795 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6914251469251944 1101 2 0.7555200245513021 1101 3 0.7430587220863458 1101 4 0.480215431469162 1101 5 0.6709301368934746 1101 6 0.5631087612035515 1101 7 0.6777431245131709 1101 8 0.6690413567651206 1101 9 0.8648315698721447 1101 10-11 0.8448128402360666 1101 12-13 0.6517105835077146 1101 14-15 0.8458160702916544 1101 16-17 0.8843749232195961 1101 18-19 0.8370715443080741 1101 20-21 0.9748629426341537 1101 22-23 0.9584368770168652 1101 24-25 0.8310486239848487 1101 26-27 0.8854189976600608 1101 28-29 0.885375780772975 1101 30-31 1.1208606569553154 1101 32-33 0.741630914654003 1101 34-35 0.8829487156245293 1101 36-37 0.8731741184043145 1101 38-39 0.743969286194357 1101 40-41 0.9339809590778501 1101 42-43 0.8332045914244404 1101 44-45 0.5785460684676238 1101 46-47 0.6458812321979011 1101 48-49 0.612438960341116 1101 50-51 0.39324078896854786 1101 52-53 0.42546398101975313 1101 54-55 0.4719849036749295 1101 56-57 0.5916819580419812 1101 58-59 0.5194748436238399 1101 60-61 0.6232928117443421 1101 62-63 1.1147384104496325 1101 64-65 1.0591436407687453 1101 66-67 0.7899092561025327 1101 68-69 0.6850508412910727 1101 70-71 0.9033314215308721 1101 72-73 1.1009939478996529 1101 74-75 0.8988282403718877 1101 76-77 1.0021994602971809 1101 78-79 1.363027730023365 1101 80-81 1.083717876863318 1101 82-83 0.9116972300012307 1101 84-85 1.1487769039763442 1101 86-87 1.3836117103755114 1101 88-89 1.4842694097118105 1101 90-91 1.39849703923365 1101 92-93 1.3296894082735307 1101 94-95 1.176307196940055 1101 96-97 1.1961230414679918 1101 98-99 1.2641511646345016 1101 100-101 0.8389324828971105 1102 1 0.11008354259508835 1102 2 -0.2573608366054252 1102 3 -0.07724170970834265 1102 4 -0.050612276370038956 1102 5 -0.27412246907867654 1102 6 -0.028839180169676126 1102 7 -0.06960833203368821 1102 8 -0.14255020195287216 1102 9 -0.0730447300731214 1102 10-11 0.022846045855480668 1102 12-13 -0.17215063403166653 1102 14-15 -0.3400563374198313 1102 16-17 -0.028676223164886494 1102 18-19 -0.03524190214329792 1102 20-21 0.12250935752194181 1102 22-23 0.04642568684474924 1102 24-25 -0.19390424349675328 1102 26-27 -0.2322298498738391 1102 28-29 -0.24496539784406934 1102 30-31 -0.05044020055243692 1102 32-33 -0.1488726430909324 1102 34-35 -0.2880358978888111 1102 36-37 -0.3516115046571642 1102 38-39 -0.29800298163998207 1102 40-41 -0.46735395547545266 1102 42-43 -0.5607168341061808 1102 44-45 -0.79856273162968 1102 46-47 -0.800004555067197 1102 48-49 -1.0295545931466847 1102 50-51 -0.9176027310703816 1102 52-53 -0.8407039931941966 1102 54-55 -0.7727879479944733 1102 56-57 -0.5903377463915191 1102 58-59 -0.6780712246118838 1102 60-61 -1.0193393113475437 1102 62-63 -0.8621240842289524 1102 64-65 -0.8076203317423563 1102 66-67 -0.968595882862374 1102 68-69 -1.0118840246093086 1102 70-71 -1.1350040403678108 1102 72-73 -1.2590255132692292 1102 74-75 -1.2358095994383653 1102 76-77 -1.179117817571818 1102 78-79 -1.0533348558794522 1102 80-81 -0.7431689442763769 1102 82-83 -0.7020745877935752 1102 84-85 -0.6644688540969987 1102 86-87 -1.360869223760595 1102 88-89 -1.2884849538471528 1102 90-91 -1.138765017568641 1102 92-93 -1.2004419546164549 1102 94-95 -1.2540947978297545 1102 96-97 -1.3579911712669137 1102 98-99 -0.8535645806674488 1102 100-101 -1.0970159450053387 1103 1 0.15881336215823794 1103 2 0.1717880237934395 1103 3 0.08252206047209398 1103 4 0.07120633711524249 1103 5 0.14079324792037795 1103 6 -0.015282674946504926 1103 7 -0.017513127855153243 1103 8 -0.04743505475439491 1103 9 0.34655997078157696 1103 10-11 0.07549917975861575 1103 12-13 0.12199407157137898 1103 14-15 0.11692965498282604 1103 16-17 -0.0871223295276522 1103 18-19 -0.15843456596095962 1103 20-21 -0.06832931769173456 1103 22-23 -0.04361764174024785 1103 24-25 0.051142646341808984 1103 26-27 -0.08458621264687594 1103 28-29 0.10263965913598838 1103 30-31 0.1431406399034003 1103 32-33 0.10230257058428549 1103 34-35 0.33484218853093495 1103 36-37 0.3608706890750284 1103 38-39 0.2739877591007627 1103 40-41 0.2666886608588257 1103 42-43 0.10816077747976749 1103 44-45 0.13573510787649212 1103 46-47 0.40510584379320136 1103 48-49 0.027280045979615863 1103 50-51 0.23799140473209235 1103 52-53 -0.028910308483837355 1103 54-55 0.12782636824483973 1103 56-57 0.23634494591617994 1103 58-59 0.42106517139951194 1103 60-61 0.3484372793457098 1103 62-63 0.3846737552772197 1103 64-65 0.17667750776382007 1103 66-67 0.44122130612147714 1103 68-69 0.5229984540231669 1103 70-71 0.6803954135733079 1103 72-73 0.5206545674525174 1103 74-75 0.6074418250630593 1103 76-77 0.8215143049353024 1103 78-79 0.9117500206637565 1103 80-81 0.7565342845935206 1103 82-83 0.4688507445900889 1103 84-85 0.5649916159884718 1103 86-87 0.5173804201140513 1103 88-89 0.6120462664757333 1103 90-91 0.8141492749289903 1103 92-93 0.9788048402553393 1103 94-95 1.1213266171749972 1103 96-97 1.3527638144880747 1103 98-99 1.5375857042328356 1103 100-101 1.9605457833897226 1104 1 0.3487283889918693 1104 2 0.2397106534893254 1104 3 0.18566383149177312 1104 4 0.17706501332275337 1104 5 0.2218867184033826 1104 6 0.2787692034076983 1104 7 0.3039202352933259 1104 8 0.20548310266957515 1104 9 0.3465935128209381 1104 10-11 0.5274110760019042 1104 12-13 0.3899222916071565 1104 14-15 0.3590249143745936 1104 16-17 0.3817171159106252 1104 18-19 0.5205289850229349 1104 20-21 0.17573933168215206 1104 22-23 0.2892479664887304 1104 24-25 0.4718436749643544 1104 26-27 0.2122720163334506 1104 28-29 0.2549484695116604 1104 30-31 0.23724283131664237 1104 32-33 0.34324621995816074 1104 34-35 0.27357765364721587 1104 36-37 0.17646773737556032 1104 38-39 0.342737759100757 1104 40-41 0.08276088805023818 1104 42-43 0.12603309411661456 1104 44-45 0.028540340434631162 1104 46-47 0.2400901908414923 1104 48-49 0.24773509964688145 1104 50-51 0.1685873349646485 1104 52-53 0.09791214232116374 1104 54-55 0.11545494606237838 1104 56-57 0.13408085825965088 1104 58-59 0.05819061862670338 1104 60-61 -0.043255475573793944 1104 62-63 -0.1326954307692887 1104 64-65 -0.27470330618967154 1104 66-67 -0.3399280677604537 1104 68-69 -0.37609591091421635 1104 70-71 -0.7219693002012875 1104 72-73 -0.5399614880036552 1104 74-75 -0.7984280318242405 1104 76-77 -0.6422099795011889 1104 78-79 -0.3182645142199654 1104 80-81 -0.2666544452812545 1104 82-83 -0.3072281910091945 1104 84-85 -0.44017944841224477 1104 86-87 -0.21672624357110237 1104 88-89 0.1683913022539123 1104 90-91 0.07683934648533963 1104 92-93 0.12824021592617996 1104 94-95 0.25655693917857647 1104 96-97 -0.062039405547707815 1104 98-99 0.1436791747158388 1104 100-101 0.17944113222693048 1105 1 0.5308361697663848 1105 2 0.44272831162376747 1105 3 0.5533550431349497 1105 4 0.5157653339171233 1105 5 0.39754190134572553 1105 6 0.5317843171423675 1105 7 0.45136311351835445 1105 8 0.4467736222360088 1105 9 0.47497374792347813 1105 10-11 0.4908175184221406 1105 12-13 0.2607990917699965 1105 14-15 0.5650602306434891 1105 16-17 0.5738091214065264 1105 18-19 0.5736891295448459 1105 20-21 0.36843690432475995 1105 22-23 0.5636691155396676 1105 24-25 0.4588417921085082 1105 26-27 0.39644076092660185 1105 28-29 0.3847128580923993 1105 30-31 0.23840816641408935 1105 32-33 0.32664401141916244 1105 34-35 0.21924454082853373 1105 36-37 0.13892257427954036 1105 38-39 -1.5064352506044543E-4 1105 40-41 -0.1741751457393903 1105 42-43 -0.0662548301620447 1105 44-45 -0.017335187528324525 1105 46-47 0.05019400235236304 1105 48-49 0.013701208241862162 1105 50-51 -0.10134803728103492 1105 52-53 -0.1342745148464104 1105 54-55 -0.027395290566801123 1105 56-57 0.142266192339342 1105 58-59 0.1262889342340472 1105 60-61 0.10921513660880322 1105 62-63 0.1587095867870687 1105 64-65 -0.08197265634996853 1105 66-67 0.02716012113578614 1105 68-69 0.00822295326572231 1105 70-71 0.33947795439135575 1105 72-73 0.21193917862908762 1105 74-75 0.049691656741227774 1105 76-77 0.02213025763687071 1105 78-79 -0.052794563581318954 1105 80-81 0.18513426776133457 1105 82-83 -0.3159682042400753 1105 84-85 -0.21645037610040063 1105 86-87 0.022022420619016714 1105 88-89 -0.27997264869006244 1105 90-91 -0.36376164074177453 1105 92-93 -0.20124428867918098 1105 94-95 0.03506693866969357 1105 96-97 0.08830917722162113 1105 98-99 -7.969333702639858E-4 1105 100-101 0.4330567243087593 1106 1 -0.05709868854500755 1106 2 0.021930192572739315 1106 3 0.009880325795933231 1106 4 0.002683156007407206 1106 5 -0.03874638569656952 1106 6 0.08757806476525332 1106 7 0.14966541876752615 1106 8 0.10854363078129126 1106 9 0.0681641989461923 1106 10-11 -0.5701478509758857 1106 12-13 -0.15515094058351053 1106 14-15 0.21669198351208507 1106 16-17 0.2434526400851169 1106 18-19 -0.06447022753415865 1106 20-21 0.07535610946576554 1106 22-23 -0.021274895603767163 1106 24-25 0.11303218879709931 1106 26-27 0.20475981096834062 1106 28-29 0.25594589719815986 1106 30-31 0.24601494533836643 1106 32-33 0.24997360722258577 1106 34-35 0.013207030517392582 1106 36-37 -0.17668465907569697 1106 38-39 0.15808764623394467 1106 40-41 0.462206003279384 1106 42-43 0.47070473042298744 1106 44-45 0.3177681214205066 1106 46-47 0.5558101761425647 1106 48-49 0.38506514321872487 1106 50-51 -0.08420808736723018 1106 52-53 -0.334068539079432 1106 54-55 -0.26747066683068965 1106 56-57 -0.0018501617413946292 1106 58-59 0.3371448944416997 1106 60-61 0.020228171861774058 1106 62-63 0.1424686198370395 1106 64-65 -0.01544297756349522 1106 66-67 -0.01325212676617582 1106 68-69 -0.3170508166835475 1106 70-71 0.048832316681490795 1106 72-73 -0.15432969630855098 1106 74-75 0.34476058700089496 1106 76-77 0.40183695209627146 1106 78-79 0.3623355172777529 1106 80-81 0.18732739103560903 1106 82-83 0.45090871906482377 1106 84-85 0.338365357089355 1106 86-87 0.012385520825979768 1106 88-89 -0.2714323105341627 1106 90-91 -0.060031355388776575 1106 92-93 0.3015144035475785 1106 94-95 0.3785055979008156 1106 96-97 0.5965778447016525 1106 98-99 0.4819174024443278 1106 100-101 0.8280683567531533 1107 1 0.24207103795883356 1107 2 0.2362172908782867 1107 3 0.12138595944865216 1107 4 -0.01310366618618275 1107 5 0.09105529810195634 1107 6 0.13464304311226272 1107 7 -0.1384792057686539 1107 8 0.11469559892668713 1107 9 0.09529098761881727 1107 10-11 0.10724439270018848 1107 12-13 -0.07827313707834094 1107 14-15 0.08873346812247718 1107 16-17 0.1786728999210041 1107 18-19 0.1358199322232565 1107 20-21 0.10724946243372102 1107 22-23 -0.09226715478085623 1107 24-25 0.0667882802143751 1107 26-27 0.09436104717484994 1107 28-29 0.1542398159534244 1107 30-31 -0.13104741721115687 1107 32-33 0.15522845371403093 1107 34-35 0.2990991627798607 1107 36-37 0.2761583252585851 1107 38-39 0.10647166468015712 1107 40-41 -0.023232724086703627 1107 42-43 -0.05672965665690555 1107 44-45 -0.18380620113638457 1107 46-47 -0.1147955262959428 1107 48-49 0.12733885251642363 1107 50-51 0.13787069657758622 1107 52-53 0.0679141385283728 1107 54-55 0.06958210446141777 1107 56-57 -0.10771972064043922 1107 58-59 -0.318788121766552 1107 60-61 -0.2920278081419099 1107 62-63 0.0895423175089789 1107 64-65 -0.05702689137780936 1107 66-67 -0.05091718843117832 1107 68-69 -0.11104949909667283 1107 70-71 0.1706097995092648 1107 72-73 0.04514181571927267 1107 74-75 -0.10503547767711652 1107 76-77 -0.11365922593297029 1107 78-79 -0.14531811238345327 1107 80-81 0.06837400067143307 1107 82-83 0.12018472445143047 1107 84-85 -0.007077342413641219 1107 86-87 0.04002093678622032 1107 88-89 0.013091372121163403 1107 90-91 -0.09084205884453311 1107 92-93 0.26894288086280227 1107 94-95 -0.08491925605049033 1107 96-97 -0.14298560776349234 1107 98-99 0.3167104153586173 1107 100-101 0.7360286160077472 1108 1 0.3404026062474834 1108 2 0.07832939908481507 1108 3 0.1400582742583083 1108 4 0.15807779898670304 1108 5 0.2635559019330316 1108 6 0.22193843727460916 1108 7 0.2620094329174094 1108 8 0.3095100834407418 1108 9 0.193910536882143 1108 10-11 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0.05918055804009015 2316 58-59 0.1951893191688896 2316 60-61 0.3054876350593645 2316 62-63 0.13518555681864086 2316 64-65 0.022444607956494167 2316 66-67 -0.012619289698882596 2316 68-69 -0.0794498563369892 2316 70-71 -0.05250207790438566 2316 72-73 -0.253937380820787 2316 74-75 -0.4427888330071923 2316 76-77 0.04552358683837099 2316 78-79 0.12667656208957467 2316 80-81 0.09908467736067905 2316 82-83 -0.01842369219663098 2316 84-85 0.05762491597365127 2316 86-87 0.09492182740618205 2316 88-89 0.2445843389523894 2316 90-91 0.24878225995936987 2316 92-93 -0.4863764440191538 2316 94-95 -0.7777442661805694 2316 96-97 -0.4637287007038644 2316 98-99 -0.7594305622559752 2316 100-101 -0.6135149102518973 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4683.0 3 602.0 4 502.0 5 454.0 6 438.0 7 481.0 8 584.0 9 739.0 10 864.0 11 1352.0 12 1943.0 13 2924.0 14 3528.0 15 4726.0 16 6074.0 17 8316.0 18 6655.0 19 4229.0 20 4148.0 21 3857.0 22 3834.0 23 3959.0 24 4136.0 25 4487.0 26 4781.0 27 5375.0 28 5936.0 29 7033.0 30 8207.0 31 9950.0 32 12170.0 33 15957.0 34 22959.0 35 44291.0 36 48702.0 37 80731.0 38 86980.0 39 12037.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.15824635038168 24.196763226143457 15.970344846546494 17.674645576928363 2 14.323057446881307 25.505639762320232 40.27850959729018 19.892793193508286 3 17.552484083116813 32.013522792337994 32.0527589644388 18.381234160106395 4 10.417146702864247 21.69185668495557 40.95999634982376 26.93100026235642 5 9.115468071562049 42.58197441344173 35.35846051697899 12.944096998017235 6 23.196023343230852 43.21966783595834 18.529659459965263 15.054649360845543 7 21.960662634522063 39.15096814818702 23.916807928616894 14.971561288674023 8 23.622930889366874 35.361861988581545 21.662450702876054 19.352756419175527 9 25.57279679940879 19.03658372470171 24.084950697848406 31.305668778041095 10-11 21.9864474840005 30.71527167775129 28.11325705289816 19.18502378535005 12-13 24.058502916929143 31.12703477490802 28.34921895684406 16.465243351318783 14-15 21.3278190977929 31.733733594445923 29.143403960714494 17.795043347046686 16-17 16.057350123186424 34.84350761930833 30.483164522310428 18.615977735194818 18-19 15.640522898181764 32.61110331698849 35.5267134998386 16.22166028499114 20-21 17.117430577262244 31.169674167737107 35.811317999603006 15.901577255397644 22-23 17.21260706549574 31.07212070953811 35.72671079512207 15.988561429844083 24-25 16.834368371751264 32.46605366834881 35.45319393877852 15.2463840211214 26-27 15.904738113081857 32.17700879591448 36.53469943204697 15.383553658956695 28-29 15.38367649575087 32.460274910169396 36.54605600866937 15.609992585410367 30-31 16.104944109128365 32.09330393249519 35.85001009560596 15.951741862770486 32-33 15.664638006855997 32.79447413601337 35.84020168719093 15.70068616993971 34-35 16.433016387271202 32.26415847683366 35.68911526987948 15.613709866015663 36-37 16.42129518725118 31.57843103703957 35.62403750727216 16.376236268437083 38-39 15.574815541287961 32.936058114472736 35.47046950016536 16.01865684407394 40-41 15.523625859176581 32.23741157338084 36.35895020510756 15.880012362335021 42-43 15.512280569706217 32.15459935475263 35.10132025960429 17.231799815936856 44-45 15.878579059402723 31.646449985805187 35.31121983032354 17.163751124468547 46-47 16.66545042405059 32.034234189036034 34.78697130503593 16.513344081877452 48-49 16.2173410351897 31.76069362316395 34.67659728762694 17.345368054019417 50-51 15.350628649353107 31.865173612575955 35.122383383179084 17.66181435489186 52-53 15.948139727654898 32.55850015221499 34.36261828016451 17.130741839965612 54-55 16.006471591582827 31.568733866627753 34.47707350888874 17.947721032900677 56-57 16.418490740719626 31.59773608827614 34.01648222596703 17.967290945037202 58-59 16.582865299849274 30.852242798213148 34.47485616949083 18.09003573244675 60-61 16.631338901399616 31.65325959921325 34.23984493030415 17.47555656908298 62-63 16.001349901550675 31.33778207982889 34.89427101325845 17.76659700536198 64-65 16.080645510692953 31.44413292850196 34.2819810893734 18.193240471431682 66-67 16.35124689615545 31.280710674945105 33.36818151761752 18.999860911281917 68-69 16.17608327585126 30.77421230553481 34.087118124241094 18.962586294372837 70-71 17.13456141211193 30.710560618380583 33.95984129934734 18.19503667016015 72-73 17.061483284962453 29.96972306008062 34.325497060685024 18.6432965942719 74-75 16.509798644860986 31.30583220633143 33.21538773806728 18.96898141074031 76-77 16.874959380925397 30.384207987904922 33.692567478972144 19.048265152197537 78-79 17.00251156883557 30.1287934322988 33.56051079586976 19.30818420299587 80-81 17.75564975294088 30.076796964604537 32.75499989739123 19.412553385063354 82-83 17.795541508754166 29.76941354604204 33.1911550757752 19.243889869428596 84-85 17.491366503744697 29.76854591287038 32.992439918960855 19.74764766442407 86-87 17.737904963416774 29.93246524650641 32.939722610462184 19.389907179614628 88-89 18.315477833253883 29.467733510937965 32.57007437954323 19.64671427626492 90-91 18.66289673012372 28.736679665267957 32.9484317847167 19.651991819891624 92-93 19.477834478433163 29.162699204607005 32.64304244946831 18.716423867491518 94-95 19.240595843693683 28.128407509637977 33.191368022446795 19.439628624221548 96-97 19.453989528222593 27.85787031281897 32.993625924206775 19.694514234751654 98-99 19.59777907269785 28.25741135847632 32.23286666795905 19.911942900866773 100-101 20.10916606716682 27.31515421419844 32.74022815251276 19.83545156612198 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7809.0 1 4516.0 2 1574.0 3 1760.0 4 1250.0 5 1234.0 6 2499.0 7 4543.0 8 6218.0 9 6207.0 10 4294.0 11 2654.5 12 2302.5 13 2184.0 14 1933.0 15 1657.5 16 1518.5 17 1480.0 18 1513.0 19 1606.0 20 1713.0 21 1841.0 22 1996.5 23 2128.5 24 2307.5 25 2740.0 26 3777.0 27 4849.5 28 5008.0 29 5494.5 30 7256.5 31 8813.5 32 10810.5 33 13707.5 34 16332.0 35 18663.5 36 21715.5 37 23704.0 38 22726.0 39 21079.5 40 19475.5 41 17706.5 42 15811.0 43 14996.0 44 14559.0 45 13626.5 46 12390.5 47 12107.0 48 14300.5 49 13357.0 50 9174.5 51 6094.0 52 4303.0 53 3444.0 54 2765.0 55 2221.5 56 1750.0 57 1394.0 58 1207.0 59 1032.0 60 825.5 61 694.0 62 587.0 63 426.0 64 336.5 65 333.0 66 311.5 67 278.5 68 232.0 69 195.0 70 185.5 71 179.0 72 161.5 73 134.0 74 102.0 75 93.5 76 87.5 77 60.5 78 43.0 79 42.0 80 30.0 81 19.5 82 15.0 83 8.5 84 5.0 85 3.5 86 3.5 87 2.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4878893995768585 2 0.049928868461370096 3 0.057680382286423 4 0.06588786751294959 5 0.07911103815568687 6 0.10646932224410886 7 0.07135952433063399 8 0.048104982855475306 9 0.0467370686510542 10-11 0.07500729554242358 12-13 0.1121689647625301 14-15 0.05551451812942292 16-17 0.06018822499452834 18-19 0.059162289341212516 20-21 0.07546326694389728 22-23 0.06440596045816006 24-25 0.09016834464142409 26-27 0.06736977456773911 28-29 0.0681677245203181 30-31 0.07170150288173927 32-33 0.07329740278689721 34-35 0.07443733129058146 36-37 0.07044758152768658 38-39 0.04080944043189611 40-41 0.044571204494054134 42-43 0.04274731888815934 44-45 0.019036806011526956 46-47 0.02621835558473773 48-49 0.02621835558473773 50-51 0.023824505727000803 52-53 0.022684577223316554 54-55 0.020974684467790182 56-57 0.02074669876705333 58-59 0.01983475596410593 60-61 0.02542040563215875 62-63 0.016756949004158462 64-65 0.019948748814474355 66-67 0.012539213540526737 68-69 0.01858083461005326 70-71 0.04297530458889619 72-73 0.039099547676369736 74-75 0.03305792660684322 76-77 0.022456591522579703 78-79 0.012197234989421464 80-81 0.014819070547895236 82-83 0.02051871306631648 84-85 0.016072991901947907 86-87 0.007979499525789743 88-89 0.044913183045159406 90-91 0.05551451812942292 92-93 0.03681969066900124 94-95 0.05790836798715984 96-97 0.047307032902896326 98-99 0.036135733566790694 100-101 0.173041146859269 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 438624.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.984129830400285 #Duplication Level Percentage of deduplicated Percentage of total 1 76.00082135846839 21.268168521124384 2 9.739496973545995 5.451026955810035 3 3.7690214875960857 3.164183599273719 4 2.0706156747893214 2.317775114886651 5 1.3703404766100067 1.9173892904653518 6 0.8994697718948743 1.5102527325136017 7 0.657405949007201 1.2877853399806511 8 0.5351948375963713 1.1981569455885481 9 0.433366144491242 1.0914637006388634 >10 3.610290431610698 21.38470761890626 >50 0.5791778715785655 11.352518814284068 >100 0.3136195225847366 15.261536557448727 >500 0.011404346275808603 2.257008670111575 >1k 0.006516769300462059 3.4268873182292 >5k 0.0032583846502310296 7.111138820738369 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9699 2.211233311446706 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8503 1.9385624133654338 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7552 1.7217480119646897 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5441 1.2404701977091996 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2888 0.6584227037280223 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2656 0.605530021157073 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2321 0.5291548114102284 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1752 0.39943094769096077 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1650 0.3761764062158021 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1371 0.31256839571022105 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1198 0.27312686948274606 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1197 0.27289888378200916 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 924 0.2106587874808492 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 870 0.1983475596410593 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 795 0.18124863208579559 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 762 0.17372510396147953 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 747 0.1703053184504268 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 746 0.17007733274968995 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 699 0.159362004815058 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 686 0.15639819070547895 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 661 0.1506985481870577 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 640 0.14591084847158386 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 622 0.14180710585832057 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 596 0.13587947763916247 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 590 0.13451156343474138 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 563 0.1283559495148464 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 487 0.11102903625884585 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 470 0.10715327934631941 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 468 0.1066973079448457 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 463 0.10555737944116146 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 458 0.10441745093747722 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.4197855110527464E-4 0.0 20-21 0.0 0.0 0.0 0.001253921354052674 0.0 22-23 0.0 0.0 0.0 0.005927628219158095 0.0 24-25 0.0 0.0 0.0 0.01766889180710586 0.0 26-27 0.0 0.0 0.0 0.03955551907784344 0.0 28-29 0.0 0.0 0.0 0.07227146713358137 0.0 30-31 0.0 0.0 0.0 0.1566261764062158 0.0 32-33 0.0 0.0 0.0 0.2812203618589042 0.0 34-35 0.0 0.0 0.0 0.43055099584154083 0.0 36-37 0.0 0.0 0.0 0.650557197052601 0.0 38-39 0.0 0.0 0.0 0.9457986795068214 0.0 40-41 0.0 0.0 0.0 1.2524394469978843 0.0 42-43 0.0 0.0 0.0 1.5207786167651565 0.0 44-45 0.0 0.0 0.0 1.8416684905522724 0.0 46-47 0.0 0.0 0.0 2.2115752899978114 0.0 48-49 0.0 0.0 0.0 2.5576575837163493 0.0 50-51 0.0 0.0 0.0 2.9072736557963084 0.0 52-53 0.0 0.0 0.0 3.23500310060553 0.0 54-55 0.0 0.0 0.0 3.584505179835121 0.0 56-57 0.0 0.0 0.0 4.006848690450135 0.0 58-59 0.0 0.0 0.0 4.412435252060991 0.0 60-61 0.0 0.0 0.0 4.821669584883637 0.0 62-63 0.0 0.0 0.0 5.222240461078281 0.0 64-65 0.0 0.0 0.0 5.629878893995768 0.0 66-67 0.0 0.0 0.0 6.085508316918363 0.0 68-69 0.0 0.0 0.0 6.552309039177063 0.0 70-71 0.0 0.0 0.0 7.05695538775808 0.0 72-73 0.0 2.2798570073684978E-4 0.0 7.524098088567886 0.0 74-75 0.0 2.2798570073684978E-4 0.0 8.035584008171007 0.0 76-77 0.0 2.2798570073684978E-4 0.0 8.540800321003868 0.0 78-79 0.0 2.2798570073684978E-4 0.0 9.129117421755307 0.0 80-81 0.0 2.2798570073684978E-4 0.0 9.752202341869118 0.0 82-83 0.0 2.2798570073684978E-4 0.0 10.315441015539506 0.0 84-85 0.0 2.2798570073684978E-4 0.0 10.868990296928576 0.0 86-87 0.0 2.2798570073684978E-4 0.0 11.458789304734808 0.0 88-89 0.0 2.2798570073684978E-4 0.0 12.07742850368425 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATAGG 55 0.0025204234 34.704597 1 GTTCGCA 95 3.4691933E-5 30.021202 9 CATATAG 150 3.3112883E-8 28.51363 3 TAGGACA 290 0.0 27.858147 4 GTATGGT 70 0.008200042 27.267897 1 CATTCCG 105 6.863153E-5 27.162039 9 CTATACC 70 0.0083664665 27.155838 4 GACAATG 195 5.293259E-10 26.813807 7 CTGTTCG 110 9.41816E-5 25.9274 7 GAGTACT 5015 0.0 25.495115 12-13 GTACATG 3375 0.0 25.025871 1 TGGGTAT 115 1.2758355E-4 24.794462 6 ATGGGGG 615 0.0 24.727268 5 GGACACC 135 1.3353054E-5 24.641409 6 ATAGCCG 155 1.3986773E-6 24.533455 8 GGACAAT 195 1.5561454E-8 24.370623 6 GTACTTT 5225 0.0 24.230562 14-15 TGGGGGA 510 0.0 24.227266 6 CATGGGG 1550 0.0 24.221256 4 GTATCAA 8460 0.0 24.197844 1 >>END_MODULE