##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938249_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 438624 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45272032538119 33.0 31.0 34.0 31.0 34.0 2 32.65370339972277 34.0 31.0 34.0 31.0 34.0 3 32.67279948201649 34.0 31.0 34.0 31.0 34.0 4 36.16145491354782 37.0 35.0 37.0 35.0 37.0 5 36.153411578025825 37.0 35.0 37.0 35.0 37.0 6 36.22845079156635 37.0 37.0 37.0 35.0 37.0 7 36.192244382432335 37.0 37.0 37.0 35.0 37.0 8 36.210469559349235 37.0 37.0 37.0 35.0 37.0 9 37.86537444371489 39.0 38.0 39.0 35.0 39.0 10-11 37.880786277084695 39.0 38.0 39.0 35.0 39.0 12-13 37.8476166374845 39.0 38.0 39.0 35.0 39.0 14-15 39.038041694024955 40.5 38.0 41.0 35.5 41.0 16-17 39.00733771977822 40.0 38.0 41.0 35.5 41.0 18-19 39.09543025461443 40.0 38.5 41.0 35.5 41.0 20-21 39.094534270810534 40.0 39.0 41.0 35.0 41.0 22-23 38.92528224629751 40.0 38.0 41.0 35.0 41.0 24-25 38.818590637995186 40.0 38.0 41.0 35.0 41.0 26-27 38.64301315021522 40.0 38.0 41.0 35.0 41.0 28-29 38.524172183920626 40.0 38.0 41.0 35.0 41.0 30-31 38.18306567812067 40.0 37.0 41.0 34.5 41.0 32-33 37.8096832366674 40.0 35.5 41.0 34.0 41.0 34-35 37.5051045998395 40.0 35.0 41.0 33.0 41.0 36-37 37.262052464069455 40.0 35.0 41.0 33.0 41.0 38-39 37.05279920843365 40.0 35.0 41.0 33.0 41.0 40-41 36.935566681257754 40.0 35.0 41.0 33.0 41.0 42-43 36.657143703946886 40.0 35.0 41.0 32.5 41.0 44-45 36.28896275625593 40.0 35.0 41.0 31.0 41.0 46-47 35.78084190559568 40.0 35.0 41.0 29.5 41.0 48-49 35.30812495440286 39.5 35.0 41.0 26.5 41.0 50-51 34.95784658386226 39.0 35.0 41.0 24.0 41.0 52-53 34.616492713577 39.0 35.0 41.0 22.5 41.0 54-55 34.388673442401696 39.0 35.0 41.0 19.5 41.0 56-57 34.12886435762749 38.5 35.0 40.5 15.5 41.0 58-59 33.79284193295396 38.0 35.0 40.0 7.0 41.0 60-61 33.39227789633034 38.0 34.0 40.0 2.0 41.0 62-63 33.02293422156562 38.0 34.0 40.0 2.0 41.0 64-65 32.62505813635369 37.0 33.0 40.0 2.0 41.0 66-67 32.12620490442839 36.5 33.0 39.5 2.0 41.0 68-69 31.59979048114102 36.0 32.5 39.0 2.0 41.0 70-71 31.07403037681477 35.5 31.5 39.0 2.0 40.0 72-73 30.647463431093602 35.0 31.0 38.0 2.0 40.0 74-75 30.192133809367476 35.0 31.0 37.0 2.0 39.5 76-77 28.841130444298535 33.5 29.0 36.0 2.0 38.5 78-79 29.457876222003357 35.0 30.5 36.0 2.0 39.0 80-81 29.221227064638505 35.0 30.0 36.0 2.0 38.0 82-83 28.827283732764283 35.0 29.5 35.5 2.0 37.0 84-85 28.430358347924418 34.5 29.0 35.0 2.0 37.0 86-87 28.068773026555775 34.0 29.0 35.0 2.0 36.0 88-89 27.63619067812067 34.0 28.5 35.0 2.0 36.0 90-91 27.336474976289487 34.0 27.0 35.0 2.0 35.5 92-93 27.004859515211205 34.0 26.5 35.0 2.0 35.0 94-95 26.678870741227108 34.0 25.5 35.0 2.0 35.0 96-97 26.211892190121837 34.0 24.5 35.0 2.0 35.0 98-99 25.716894196396 33.0 22.0 35.0 2.0 35.0 100-101 23.661096975997665 31.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0884648439979614 1101 2 0.1933881214800337 1101 3 0.09597210281098967 1101 4 0.05077355226519842 1101 5 0.10677918312704548 1101 6 0.13562512995096654 1101 7 0.16984092037051113 1101 8 0.1924119639391222 1101 9 0.2680483432700953 1101 10-11 0.3160714096583419 1101 12-13 0.28997914375729295 1101 14-15 0.24747653616298493 1101 16-17 0.3030153123886308 1101 18-19 0.3501829844469029 1101 20-21 0.3808605680764501 1101 22-23 0.5070981067037934 1101 24-25 0.2619209837212395 1101 26-27 0.4598105213982322 1101 28-29 0.3988572640014141 1101 30-31 0.2660389503983325 1101 32-33 0.21633497109865374 1101 34-35 -0.014981503665751461 1101 36-37 -0.007814555111700372 1101 38-39 0.24727944528861912 1101 40-41 -0.17825340773557485 1101 42-43 -0.11079909012297406 1101 44-45 -0.06459362872806906 1101 46-47 0.12643540201958103 1101 48-49 -0.22003912396977654 1101 50-51 -0.6099235684916096 1101 52-53 -0.9046258785920074 1101 54-55 -0.8541771165154657 1101 56-57 -0.8679424582697237 1101 58-59 -0.7294163495215003 1101 60-61 -0.6082747045701922 1101 62-63 -0.4684363843448267 1101 64-65 -0.4206755388814756 1101 66-67 -0.17952072014079334 1101 68-69 -0.08377824082019814 1101 70-71 -0.056555067874246134 1101 72-73 -0.2830378344464357 1101 74-75 -0.1846478953068491 1101 76-77 -0.21473308253768408 1101 78-79 -0.2486793452029623 1101 80-81 -0.05417446759424749 1101 82-83 0.056900228010700715 1101 84-85 -0.047173391791613284 1101 86-87 0.028346627570261518 1101 88-89 -0.08122454589620887 1101 90-91 -0.17364097161807734 1101 92-93 -0.05222337144049405 1101 94-95 -0.04635065395856941 1101 96-97 0.2215260243256587 1101 98-99 0.006339642898034015 1101 100-101 0.5051016049631265 1102 1 -0.023753121093562868 1102 2 -0.0563412466401374 1102 3 -0.035439440094805263 1102 4 -0.11905555434552184 1102 5 -0.07525572534464686 1102 6 -0.1400301640951298 1102 7 -0.20208634351077848 1102 8 -0.17941191248245048 1102 9 -0.14890111850694865 1102 10-11 -0.0846431801367018 1102 12-13 -0.13680428992269356 1102 14-15 -0.16408765528626645 1102 16-17 -0.23695914482720326 1102 18-19 -0.26102847331628 1102 20-21 -0.20927201113659777 1102 22-23 -0.04486017341541526 1102 24-25 -0.17855216594730905 1102 26-27 -0.24232412556400362 1102 28-29 -0.06791279012496432 1102 30-31 -0.05994171006508964 1102 32-33 -0.250167491955537 1102 34-35 -0.13966373926752595 1102 36-37 -0.155272439925902 1102 38-39 -0.2778072177290838 1102 40-41 0.01380400271264648 1102 42-43 -0.04393781176744227 1102 44-45 -0.45157593122760886 1102 46-47 -0.603337145569057 1102 48-49 -0.598793000999521 1102 50-51 -0.8707944557992846 1102 52-53 -0.6794358281145989 1102 54-55 -0.8466511176101577 1102 56-57 -0.5931659576311503 1102 58-59 -1.090944051279081 1102 60-61 -1.3643411158090188 1102 62-63 -1.3115094245443046 1102 64-65 -1.3558972137583254 1102 66-67 -1.5845289303214116 1102 68-69 -1.4663939435508446 1102 70-71 -1.2045207675640874 1102 72-73 -1.3474147729613009 1102 74-75 -1.6733573765450664 1102 76-77 -1.220492372205019 1102 78-79 -1.318417227584522 1102 80-81 -1.1067713173175342 1102 82-83 -1.1421023870838702 1102 84-85 -1.2807530305436643 1102 86-87 -0.8972965032452827 1102 88-89 -1.0563750658743167 1102 90-91 -1.596589946865901 1102 92-93 -1.5163631269595612 1102 94-95 -1.6397095300760682 1102 96-97 -1.3180622503200858 1102 98-99 -1.3584417230327581 1102 100-101 -1.1042215112565437 1103 1 0.11847147529769586 1103 2 0.09099374861152398 1103 3 -0.05735302034172207 1103 4 0.10079249883586527 1103 5 0.06168634493076297 1103 6 0.15342306477476342 1103 7 -0.05057874616984748 1103 8 0.026274222370346934 1103 9 -0.011650406256237034 1103 10-11 -0.0050966464235031594 1103 12-13 -0.010566754310161741 1103 14-15 0.03193561157033287 1103 16-17 0.12127625403319797 1103 18-19 0.12159260930479832 1103 20-21 0.3857570724341528 1103 22-23 0.31582655497965106 1103 24-25 0.2509023687155576 1103 26-27 0.16088100264111915 1103 28-29 0.11140226210675763 1103 30-31 0.08027374577536506 1103 32-33 0.022428661890614876 1103 34-35 0.03781429966884531 1103 36-37 -0.04578466689646632 1103 38-39 0.03838758284071986 1103 40-41 0.24642805304502957 1103 42-43 0.05557548357918307 1103 44-45 -0.08435727025894835 1103 46-47 0.007634483110905421 1103 48-49 0.15004068845917118 1103 50-51 0.09807425265275782 1103 52-53 0.10494213959670873 1103 54-55 0.09178371144967201 1103 56-57 0.1119978600326661 1103 58-59 0.054687477685774866 1103 60-61 0.29092953946163647 1103 62-63 0.31749105028950453 1103 64-65 0.05323740162629065 1103 66-67 0.2730832491657651 1103 68-69 0.5077929035526729 1103 70-71 0.4512840757407588 1103 72-73 0.48914149484496505 1103 74-75 0.29677854463252373 1103 76-77 0.30768307888709856 1103 78-79 0.43626878855982554 1103 80-81 0.6393765934137079 1103 82-83 0.6289208797727319 1103 84-85 0.48407068740505466 1103 86-87 0.48232422325044055 1103 88-89 0.586707199082948 1103 90-91 0.5786266960590751 1103 92-93 0.6659641522427187 1103 94-95 0.1774557120891771 1103 96-97 0.00838302508352129 1103 98-99 0.0372639987146286 1103 100-101 0.11269444315941257 1104 1 0.1111593107180937 1104 2 0.06866593108021846 1104 3 0.08276325872804335 1104 4 0.02458498541905385 1104 5 -0.04350149048963203 1104 6 0.03648433489998837 1104 7 0.0748013969428527 1104 8 0.060151682119901295 1104 9 0.0837431536722022 1104 10-11 0.1345500440952705 1104 12-13 0.09192094694207498 1104 14-15 0.28795081729484195 1104 16-17 0.08801932201172491 1104 18-19 0.36407919248905785 1104 20-21 0.2822295679618847 1104 22-23 0.22113178753778584 1104 24-25 0.17796520413594408 1104 26-27 0.30224951784684606 1104 28-29 -0.02903825667679172 1104 30-31 0.10797387994352192 1104 32-33 0.2637859964165514 1104 34-35 0.47318438016973374 1104 36-37 0.5103032132466438 1104 38-39 0.6457500604793651 1104 40-41 0.6861697793419879 1104 42-43 0.6115459665845506 1104 44-45 0.5411458603313903 1104 46-47 0.40873578006976174 1104 48-49 0.39388572423735013 1104 50-51 0.2172043957654637 1104 52-53 0.22363623619777684 1104 54-55 0.11407239660172763 1104 56-57 0.4929236203188836 1104 58-59 0.4520935599755802 1104 60-61 0.5369939401771973 1104 62-63 0.5470751290014917 1104 64-65 0.19759115833470275 1104 66-67 0.13485091463982002 1104 68-69 -0.05930235583909749 1104 70-71 -0.0897490190714052 1104 72-73 -0.07084285220333442 1104 74-75 0.2857152619849366 1104 76-77 -0.19384867424349395 1104 78-79 -0.11181372485699015 1104 80-81 -0.25245032966321546 1104 82-83 -0.0726220540376481 1104 84-85 0.1339756516268764 1104 86-87 0.12311693344722485 1104 88-89 -0.13479883848413365 1104 90-91 -0.1455850660160607 1104 92-93 -0.03143633970719506 1104 94-95 -0.4104244310235252 1104 96-97 -0.2068506511239896 1104 98-99 0.060234705333591876 1104 100-101 0.15305669718445714 1105 1 0.00720022466649084 1105 2 0.009931006648891127 1105 3 0.0441240037282995 1105 4 0.08320286746067751 1105 5 0.04754731109807153 1105 6 0.09549405450712811 1105 7 0.012636621613353327 1105 8 -0.013228807422613897 1105 9 0.21573134765723978 1105 10-11 0.11313891997945547 1105 12-13 -0.038879012347528885 1105 14-15 -0.07449638640980538 1105 16-17 0.0320577933363424 1105 18-19 -0.13105845953882067 1105 20-21 -0.01588248740425513 1105 22-23 -0.16314636624573353 1105 24-25 -0.1331054792898314 1105 26-27 -0.17346927508990007 1105 28-29 -0.20806731372223197 1105 30-31 -0.09691391202635202 1105 32-33 -0.010999001802368014 1105 34-35 -0.1576257567189714 1105 36-37 -0.011660045620587312 1105 38-39 -0.10137476777365606 1105 40-41 0.13477544826469057 1105 42-43 0.004037442845302053 1105 44-45 0.11291065601609063 1105 46-47 0.30904365244266785 1105 48-49 0.2934429986763121 1105 50-51 0.21346412809459991 1105 52-53 0.06955247455714897 1105 54-55 0.05512374258920971 1105 56-57 -0.0014405341770711289 1105 58-59 0.053635466239896346 1105 60-61 -0.1924065992355537 1105 62-63 0.0111558373623879 1105 64-65 0.2828336396333597 1105 66-67 0.08075188660053811 1105 68-69 0.0637651725309567 1105 70-71 0.34767146843558905 1105 72-73 0.20318900368186021 1105 74-75 0.07967231251671691 1105 76-77 0.23265888262384138 1105 78-79 0.2533687086069598 1105 80-81 0.23442056749214402 1105 82-83 0.30941448354314005 1105 84-85 0.3862783334242401 1105 86-87 0.3536395531612335 1105 88-89 0.5429198526766186 1105 90-91 0.3920287958739195 1105 92-93 0.1333491673916818 1105 94-95 0.19725456750072112 1105 96-97 0.427643776640501 1105 98-99 0.48239897178313385 1105 100-101 0.7109417920905692 1106 1 -0.11111482440711029 1106 2 -0.016104923032862928 1106 3 -0.08867356210244282 1106 4 -0.10297551644456604 1106 5 -0.13522284069174617 1106 6 -0.07951125541971749 1106 7 0.05320446272058632 1106 8 -0.033816505186521795 1106 9 0.008426864103199705 1106 10-11 -0.03108857210504823 1106 12-13 -4.277327786468277E-4 1106 14-15 -0.06676859054808659 1106 16-17 -0.25563651294023515 1106 18-19 -0.2491314747375668 1106 20-21 -0.07932746915292199 1106 22-23 -0.10329731635218309 1106 24-25 -0.033812316993774516 1106 26-27 -0.11583804580478585 1106 28-29 -0.09313085996304693 1106 30-31 0.029741426796377368 1106 32-33 -0.044298417463437545 1106 34-35 -0.03447743934834335 1106 36-37 -0.2015924243196352 1106 38-39 -0.14341210026397988 1106 40-41 -0.175951591858599 1106 42-43 -0.11399605661876677 1106 44-45 -0.04443605997938249 1106 46-47 -0.27166476589066235 1106 48-49 -0.36196497658684024 1106 50-51 -0.42674016825364447 1106 52-53 -0.2939552384203168 1106 54-55 -0.5771502397571382 1106 56-57 -0.32285411079561044 1106 58-59 -0.15381226185233743 1106 60-61 -0.011987633658698371 1106 62-63 -0.04417113064468481 1106 64-65 -0.4798249789953388 1106 66-67 -0.315981150035487 1106 68-69 -0.4765631044348275 1106 70-71 -0.5830426302845915 1106 72-73 0.07291797513668641 1106 74-75 0.15126988427482146 1106 76-77 -0.04362031579947967 1106 78-79 -0.16172185651744186 1106 80-81 -0.39691985562258125 1106 82-83 -0.4079887399529163 1106 84-85 0.19817849692059397 1106 86-87 -0.19002286391746637 1106 88-89 -0.14977862744943593 1106 90-91 0.11392199472203757 1106 92-93 0.19300063861188121 1106 94-95 0.15451336098655233 1106 96-97 -0.14251394053550825 1106 98-99 0.12992602771272033 1106 100-101 0.38705848205505333 1107 1 0.07322139276220696 1107 2 0.05481225306844095 1107 3 -0.002095509612111357 1107 4 -0.031099214600907032 1107 5 0.10344930300273347 1107 6 0.12795054909302195 1107 7 0.002099530489118706 1107 8 0.030008953304651698 1107 9 -0.004758692819464727 1107 10-11 0.04861332386371231 1107 12-13 0.013879226211464868 1107 14-15 0.1509311546538612 1107 16-17 0.048464476218732955 1107 18-19 0.038892048063473794 1107 20-21 0.1485036472113066 1107 22-23 0.17895691978651485 1107 24-25 0.15569252223156127 1107 26-27 0.13872022350609114 1107 28-29 -0.016352359880706047 1107 30-31 0.036060515336281185 1107 32-33 -0.1838584790800013 1107 34-35 -0.46395505490362154 1107 36-37 -0.4418776431262472 1107 38-39 -0.6634374831876713 1107 40-41 -0.6782428366875521 1107 42-43 -0.7861833477182714 1107 44-45 -0.6736709876574309 1107 46-47 -0.7470132966844005 1107 48-49 -0.8826588410885279 1107 50-51 -0.8574288011603812 1107 52-53 -0.7353516867486221 1107 54-55 -0.6210907933373875 1107 56-57 -0.6341848735427718 1107 58-59 -0.6046885540078506 1107 60-61 -0.4764854489833894 1107 62-63 -0.7631254898227482 1107 64-65 -0.8264980721274213 1107 66-67 -0.9645003180181249 1107 68-69 -0.5847190640934166 1107 70-71 -0.7699316720307827 1107 72-73 -0.9142403968684683 1107 74-75 -0.683273659065069 1107 76-77 -0.6257829990264057 1107 78-79 -0.5466824742331724 1107 80-81 -0.6301327330966906 1107 82-83 -0.39103207499782044 1107 84-85 -0.2414111135193444 1107 86-87 -0.21923260243460163 1107 88-89 -0.2514871307288722 1107 90-91 -0.30527166148466733 1107 92-93 -0.3910730299956455 1107 94-95 -0.7038169851706471 1107 96-97 -0.6572399115292207 1107 98-99 -0.1391814040585615 1107 100-101 -0.17826379388251823 1108 1 0.04106971039593077 1108 2 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2316 64-65 -0.2088837262824086 2316 66-67 0.04437280378262898 2316 68-69 -0.034823259186321565 2316 70-71 -0.05722896709309211 2316 72-73 0.12391898854587069 2316 74-75 -0.2929534658906725 2316 76-77 -0.21940766514728693 2316 78-79 -0.1339313930023458 2316 80-81 -0.058808711166104644 2316 82-83 -0.0609134910587521 2316 84-85 -0.11890331956100653 2316 86-87 -0.022601577105273662 2316 88-89 -0.19921385842409123 2316 90-91 -0.23190715231798364 2316 92-93 -0.2538497465271057 2316 94-95 -0.20325052772111007 2316 96-97 -0.3781707210258567 2316 98-99 -0.41423991043017594 2316 100-101 -0.7479380601754038 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 0.0 4 7.0 5 17.0 6 47.0 7 116.0 8 231.0 9 408.0 10 683.0 11 1182.0 12 1822.0 13 2623.0 14 3415.0 15 4469.0 16 5690.0 17 7798.0 18 7262.0 19 4803.0 20 3906.0 21 3819.0 22 3923.0 23 4372.0 24 4659.0 25 4989.0 26 4899.0 27 4631.0 28 5547.0 29 6330.0 30 7585.0 31 9064.0 32 10994.0 33 13842.0 34 20497.0 35 40389.0 36 44968.0 37 84887.0 38 105703.0 39 13045.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.83976253009411 24.759247100021888 16.22209455022981 18.17889581965419 2 14.279036465459056 25.563445155876614 39.95056458527153 20.206953793392803 3 17.3390332877337 32.02279981760146 32.49726402188782 18.14090287277702 4 10.202588093674764 22.483493835266653 40.103824688115566 27.210093382943022 5 8.816435033194718 43.01725395783176 35.612734369300355 12.553576639673159 6 23.007860946961404 43.17729991974903 18.264618443131248 15.550220690158314 7 21.557415918873566 39.41029218647407 23.327724885095208 15.704567009557161 8 23.63675328642886 35.72780207103155 21.561389989558208 19.07405465298138 9 24.688171846893574 19.014962735652258 23.560779986457618 32.73608543099655 10-11 21.72532738746626 30.868123951265776 27.92026884073831 19.486279820529656 12-13 23.887201794703437 30.903689720580722 28.14688662727074 17.0622218574451 14-15 21.064510833880497 31.955501750930182 28.557488874297803 18.422498540891517 16-17 16.065828666270733 35.035628505996605 30.042393989378134 18.85614883835453 18-19 15.555464361275263 32.731907054789524 34.92490151017728 16.78772707375793 20-21 16.995194061428467 31.222527723061212 35.24921116947545 16.533067046034873 22-23 16.991774275917415 31.548205296563797 35.1876550302765 16.272365397242286 24-25 16.8245202830877 32.9527214358675 34.85916295782869 15.363595323216108 26-27 15.892241841288518 32.63792446849509 36.20664098933 15.263192700886394 28-29 14.93101152695703 32.62452578974247 36.32108594148975 16.123376741810755 30-31 15.893225176916904 32.146667760998035 35.89817246662289 16.061934595462173 32-33 15.426187984615419 33.24658418087597 35.59667685769719 15.730550976811417 34-35 16.05771171280054 32.606273993813346 35.79435128481989 15.541663008566225 36-37 16.337058055043194 31.77876931425145 35.58541818190109 16.298754448804267 38-39 15.250339482982525 32.879739222341684 35.48808588605913 16.381835408616656 40-41 15.19179725267709 32.3415865516172 36.39153912107558 16.075077074630133 42-43 15.54776521823395 32.04610359628572 35.101549897110544 17.304581288369786 44-45 15.451321400845632 31.821347836842364 35.33611562984132 17.391215132470677 46-47 16.678901927167523 31.769423856022144 34.617313729558184 16.93436048725215 48-49 16.074245823301965 31.53464014736996 34.50495184942001 17.886162179908077 50-51 15.002143065586926 31.674509374772015 35.197230429707446 18.12611712993361 52-53 15.672535018603634 32.5312796381411 34.45867075217042 17.337514591084847 54-55 15.716422265995478 31.631078098781646 34.23752462245568 18.414975012767197 56-57 16.337113336251548 31.81734241628365 33.50512055883855 18.340423688626252 58-59 16.029902604508646 31.587076858539433 34.22692328737141 18.156097249580505 60-61 16.288894360545704 32.81022014299263 33.444932333844015 17.45595316261764 62-63 15.350277230612097 32.71230028452615 34.00235737214562 17.93506511271613 64-65 15.34925129495878 32.72221766250821 33.3031252279857 18.62540581454731 66-67 15.592512037644997 32.486708433647046 32.419224666228935 19.501554862479026 68-69 15.422469865921304 31.829156245796504 33.17598484347606 19.572389044806133 70-71 16.2842206536806 31.692862223681328 32.885683409936526 19.13723371270154 72-73 16.221980557379442 30.793458634274458 33.31167469176333 19.67288611658277 74-75 15.584874516670313 32.0688106441964 32.112583898737874 20.233730940395418 76-77 15.759967534836214 31.112524622455677 32.99044283942511 20.137065003282995 78-79 15.793937404246005 30.78171737068651 32.91577752243379 20.50856770263369 80-81 16.332211643685707 30.993972058072515 31.968952907273657 20.704863390968118 82-83 16.18071514554607 30.538114649449188 32.62418381119136 20.65698639381338 84-85 15.71771046718808 30.639503800042633 32.25846901657154 21.384316716197745 86-87 15.733005032801556 30.93366163386511 32.3698624671557 20.963470866177637 88-89 16.06136463121033 30.4181941708616 31.893147661778652 21.627293536149413 90-91 16.06314776732585 29.656971150557883 32.48377874342828 21.79610233868798 92-93 16.574142258841874 30.217407659881033 32.172845473219596 21.03560460805749 94-95 15.891059312759904 29.237456226745458 32.80611640037937 22.06536806011527 96-97 15.827109323703217 29.518448602903625 32.6442465528562 22.01019552053695 98-99 15.674358904209528 29.791689465236743 32.047038009776024 22.486913620777706 100-101 15.430756182972205 29.227310863062666 32.765078974246734 22.576853979718393 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7667.0 1 4437.0 2 1474.0 3 1655.0 4 1239.5 5 1241.0 6 2554.5 7 4567.0 8 5983.5 9 6022.0 10 4322.5 11 2666.5 12 2267.0 13 2069.0 14 1875.0 15 1677.0 16 1504.5 17 1432.0 18 1442.0 19 1517.0 20 1589.0 21 1738.0 22 1851.5 23 1944.0 24 2175.5 25 2636.5 26 3692.0 27 4762.0 28 4913.0 29 5459.5 30 7186.5 31 8738.0 32 10962.0 33 13990.0 34 16501.0 35 18695.5 36 21749.5 37 23863.5 38 22920.5 39 21363.5 40 19936.0 41 18132.0 42 16284.5 43 15764.5 44 15498.5 45 14723.0 46 13124.5 47 12655.5 48 15103.0 49 13869.0 50 9537.5 51 6221.5 52 3834.5 53 2714.0 54 2138.0 55 1768.5 56 1480.5 57 1117.0 58 880.5 59 751.5 60 592.0 61 453.5 62 333.0 63 199.5 64 125.0 65 114.5 66 106.5 67 96.0 68 81.0 69 63.0 70 52.0 71 47.5 72 50.5 73 49.0 74 42.0 75 46.5 76 33.0 77 14.5 78 20.5 79 33.5 80 21.0 81 3.5 82 1.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.016414970453053183 3 0.005471656817684395 4 0.0 5 0.0 6 0.0 7 0.0 8 4.5597140147369956E-4 9 2.2798570073684978E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.1399285036842489E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.007295542423579193 26-27 0.023938498577369228 28-29 0.0 30-31 0.0 32-33 6.839571022105494E-4 34-35 0.022684577223316554 36-37 0.0052436711169475455 38-39 0.020404720215948056 40-41 0.02006274166484278 42-43 0.010601335084263514 44-45 0.003989749762894871 46-47 2.2798570073684978E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.2798570073684978E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0023938498577369227 86-87 3.419785511052747E-4 88-89 0.0 90-91 4.5597140147369956E-4 92-93 2.2798570073684978E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 438624.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.411220165678543 #Duplication Level Percentage of deduplicated Percentage of total 1 70.04287670842868 16.397892076585016 2 12.075796326891949 5.654182529695194 3 4.781073112856872 3.357922658198948 4 2.603238531069817 2.437799615786124 5 1.67753478918141 1.9636568142555564 6 1.1406092735497313 1.6021832895652455 7 0.8382397788068775 1.3736951209294246 8 0.6456438887879027 1.2092248983230771 9 0.48997083327229723 1.0323733547248855 >10 4.52649221815097 22.110638266208316 >50 0.7444363303104117 12.218870941580702 >100 0.4051489947801972 16.6457644728417 >500 0.014968558920450637 2.515636157296872 >1k 0.009979039280300426 4.300971550504775 >5k 0.00399161571212017 7.179188253504168 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8978 2.046855621215437 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8556 1.9506456555044867 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7672 1.7491062960531114 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5524 1.2593930108703582 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2692 0.6137375063835996 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2555 0.5825034653826512 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2444 0.5571970526008609 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 2200 0.5015685416210695 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1755 0.4001149047931714 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1572 0.3583935215583279 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1410 0.3214598380389582 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 1349 0.3075527102940104 TruSeq Adapter, Index 11 (95% over 21bp) GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1246 0.2840701831181148 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1187 0.27061902677464067 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 978 0.2229700153206391 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 916 0.20883490187495443 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 805 0.18352848909316408 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 781 0.1780568322754797 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 779 0.17760086087400598 RNA PCR Primer, Index 11 (95% over 23bp) TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 765 0.17440906106369009 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 740 0.16870941854526886 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 676 0.15411833369811045 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 667 0.1520664623914788 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 664 0.15138250528926825 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 655 0.1493306339826366 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 626 0.14271904866126797 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 617 0.14066717735463632 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 573 0.1306358065222149 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 526 0.11992047858758298 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 467 0.10646932224410886 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 462 0.1053293937404246 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 460 0.10487342233895092 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 456 0.10396147953600351 No Hit GAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 449 0.10236557963084557 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 442 0.1007696797256876 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.4197855110527464E-4 0.0 20-21 0.0 0.0 0.0 0.001139928503684249 0.0 22-23 0.0 0.0 0.0 0.005927628219158095 0.0 24-25 0.0 0.0 0.0 0.01789687750784271 0.0 26-27 0.0 0.0 0.0 0.03932753337710659 0.0 28-29 0.0 0.0 0.0 0.07181549573210769 0.0 30-31 0.0 0.0 0.0 0.1570821478076895 0.0 32-33 0.0 0.0 0.0 0.2844121616692201 0.0 34-35 0.0 0.0 0.0 0.4363646312103305 0.0 36-37 0.0 0.0 0.0 0.656484825271759 0.0 38-39 0.0 0.0 0.0 0.953208214780769 0.0 40-41 0.0 0.0 0.0 1.262242832129569 0.0 42-43 0.0 0.0 0.0 1.5344577588093675 0.0 44-45 0.0 0.0 0.0 1.8587674181075362 0.0 46-47 0.0 0.0 0.0 2.2320940030641276 0.0 48-49 0.0 0.0 0.0 2.5830779893485083 0.0 50-51 0.0 0.0 0.0 2.939875611001678 0.0 52-53 0.0 0.0 0.0 3.2737606697307946 0.0 54-55 0.0 0.0 0.0 3.6278224629751223 0.0 56-57 0.0 0.0 0.0 4.053927737652295 0.0 58-59 0.0 0.0 0.0 4.463276063325308 0.0 60-61 0.0 0.0 0.0 4.877184103013059 0.0 62-63 0.0 0.0 0.0 5.281972714671335 0.0 64-65 0.0 0.0 0.0 5.692802947399139 0.0 66-67 0.0 0.0 0.0 6.150256255927628 0.0 68-69 0.0 0.0 0.0 6.619678813744802 0.0 70-71 0.0 0.0 0.0 7.1262630407820815 0.0 72-73 0.0 0.0 0.0 7.597737469905887 0.0 74-75 0.0 0.0 0.0 8.113783103523748 0.0 76-77 0.0 0.0 0.0 8.624015101772816 0.0 78-79 0.0 0.0 0.0 9.218715802144889 0.0 80-81 0.0 0.0 0.0 9.847386371926753 0.0 82-83 0.0 0.0 0.0 10.414386809659298 0.0 84-85 0.0 0.0 0.0 10.974547676369738 0.0 86-87 0.0 0.0 0.0 11.57084427664697 0.0 88-89 0.0 0.0 0.0 12.198602903625885 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGCGTC 25 0.0050170887 57.00609 2 GTCTAGA 25 0.0050170887 57.00609 1 AGCGTCA 25 0.0050193537 56.99959 3 CTGAACG 25 0.0050216196 56.99309 9 GCGTCAG 25 0.0050216196 56.99309 4 CGTCAGA 25 0.0050216196 56.99309 5 GGTGTGC 35 2.787706E-4 54.279137 8 GTATGGT 60 1.4890611E-6 47.505074 1 GGACTGA 50 0.0016156881 37.995396 6 AGGAGCC 55 0.0025794269 34.541267 7 ATCTAGA 60 0.003946247 31.670048 1 GGATATG 150 9.513315E-10 31.670048 1 CGGGGCC 30 0.0039527914 31.662827 10-11 CTTGCGT 30 0.0039527914 31.662827 58-59 ACCCTTG 170 1.07320375E-10 30.73157 7 AGGGTCT 110 2.7309488E-6 30.223608 5 TAGGGTC 110 2.7309488E-6 30.223608 4 CTTCGAT 80 4.48935E-4 29.687286 3 TTCGATA 80 4.4923488E-4 29.6839 4 ATGGGTC 85 6.4053515E-4 27.93779 5 >>END_MODULE