##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938248_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 445764 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.90184043574627 33.0 31.0 34.0 30.0 34.0 2 31.98249522168681 33.0 31.0 34.0 30.0 34.0 3 31.95009691226748 34.0 31.0 34.0 30.0 34.0 4 35.46920343500148 37.0 35.0 37.0 35.0 37.0 5 35.50039707109592 37.0 35.0 37.0 35.0 37.0 6 35.58662206907691 37.0 36.0 37.0 35.0 37.0 7 35.538363349216176 37.0 36.0 37.0 35.0 37.0 8 35.54799400579679 37.0 36.0 37.0 35.0 37.0 9 37.17203049147082 39.0 38.0 39.0 35.0 39.0 10-11 37.13307602228982 39.0 37.5 39.0 35.0 39.0 12-13 37.02052319164401 39.0 37.0 39.0 34.0 39.0 14-15 38.1545358081855 40.0 38.0 41.0 33.5 41.0 16-17 38.046339542897144 40.0 38.0 41.0 33.5 41.0 18-19 38.23248624832871 40.0 38.0 41.0 34.0 41.0 20-21 38.2703919562818 40.0 38.0 41.0 34.0 41.0 22-23 38.13515447635969 40.0 38.0 41.0 34.0 41.0 24-25 37.99332718658303 40.0 38.0 41.0 34.0 41.0 26-27 37.7742628386321 40.0 37.5 41.0 34.0 41.0 28-29 37.58372703941996 40.0 37.0 41.0 33.0 41.0 30-31 37.25301841333082 40.0 36.5 41.0 33.0 41.0 32-33 36.81310065415781 39.0 35.0 41.0 33.0 41.0 34-35 36.52133079387299 39.0 35.0 41.0 32.0 41.0 36-37 36.22212874974201 39.0 35.0 41.0 31.0 41.0 38-39 35.915971231413934 38.5 35.0 41.0 30.5 41.0 40-41 35.66495051193008 39.0 35.0 41.0 30.0 41.0 42-43 35.34303802011827 39.0 35.0 41.0 28.0 41.0 44-45 34.90305071741998 38.5 35.0 41.0 24.5 41.0 46-47 34.375025798404536 38.0 35.0 40.5 21.5 41.0 48-49 33.89284240091169 38.0 35.0 40.0 12.0 41.0 50-51 33.140608034744844 37.5 33.5 39.5 4.5 40.5 52-53 33.20954922335585 38.0 33.5 40.0 2.0 40.5 54-55 33.301555307292645 38.0 34.5 40.0 2.0 41.0 56-57 33.04276029468508 38.0 34.0 40.0 2.0 41.0 58-59 32.73368082662574 38.0 33.5 40.0 2.0 41.0 60-61 32.31953679525489 37.5 33.0 40.0 2.0 41.0 62-63 31.8880237076121 37.0 33.0 40.0 2.0 41.0 64-65 31.48818208738256 36.5 32.0 39.5 2.0 41.0 66-67 31.036140199746953 36.0 31.0 39.0 2.0 41.0 68-69 30.480689333369227 35.0 30.5 39.0 2.0 40.0 70-71 30.00851347349719 35.0 30.0 38.5 2.0 40.0 72-73 29.386924471244875 35.0 29.0 37.5 2.0 39.5 74-75 28.80870258701914 35.0 28.0 37.0 2.0 39.0 76-77 28.182242172988396 34.5 26.5 36.0 2.0 39.0 78-79 27.52641980958534 34.0 26.0 36.0 2.0 38.0 80-81 26.873570992722605 34.0 24.5 35.0 2.0 37.0 82-83 26.22964842382965 33.0 22.0 35.0 2.0 37.0 84-85 25.573962904137616 33.0 19.0 35.0 2.0 36.0 86-87 24.99080567295699 32.5 14.0 35.0 2.0 36.0 88-89 24.21193950161969 31.5 4.5 35.0 2.0 35.0 90-91 23.45228753331359 31.0 2.0 34.5 2.0 35.0 92-93 22.65370689423103 31.0 2.0 34.0 2.0 35.0 94-95 21.75357476153301 30.0 2.0 34.0 2.0 35.0 96-97 20.676839089742554 29.0 2.0 34.0 2.0 35.0 98-99 19.377982519898424 28.0 2.0 34.0 2.0 35.0 100-101 15.398802954029486 12.5 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2601608290247839 1101 2 0.32449535047485156 1101 3 0.1630598925385236 1101 4 0.23873510644854434 1101 5 0.27667415382449434 1101 6 0.2605195605247701 1101 7 0.4883144682327156 1101 8 0.31499775081018555 1101 9 0.18239235699603995 1101 10-11 0.3976747360062376 1101 12-13 0.4582671685593098 1101 14-15 0.31449406306376915 1101 16-17 0.4566464312644243 1101 18-19 0.5687262203543142 1101 20-21 0.5188415162973357 1101 22-23 0.5757153690403314 1101 24-25 0.41095659818243746 1101 26-27 0.37313093363699323 1101 28-29 0.49105528184961855 1101 30-31 0.4464564683718919 1101 32-33 0.2894833357754223 1101 34-35 0.4488353251688437 1101 36-37 0.3199773042703171 1101 38-39 0.28474923107471994 1101 40-41 0.2873647056720898 1101 42-43 0.48101616685040227 1101 44-45 0.2759442732050985 1101 46-47 0.5105466022888905 1101 48-49 0.28659592382338417 1101 50-51 -0.2045393079803759 1101 52-53 -0.1341470196363943 1101 54-55 0.012679349248514882 1101 56-57 0.07242220284610568 1101 58-59 -0.04635379455609012 1101 60-61 -0.19898801184497472 1101 62-63 -0.23115518604361185 1101 64-65 -0.6115587581461774 1101 66-67 -0.649018367072653 1101 68-69 -0.45211764197207316 1101 70-71 -0.4737706611269843 1101 72-73 -0.5828459983845562 1101 74-75 -0.7757352016591241 1101 76-77 -0.9954336119781821 1101 78-79 -1.2939939350997705 1101 80-81 -1.0643605097368685 1101 82-83 -1.1902182998728748 1101 84-85 -1.3124955597319001 1101 86-87 -0.8728667047163299 1101 88-89 -1.0592269362362359 1101 90-91 -1.3440492671762492 1101 92-93 -1.249706794496042 1101 94-95 -1.10262194629723 1101 96-97 -1.1608336641354917 1101 98-99 -1.1127221165705201 1101 100-101 -1.269121814089134 1102 1 -0.13183985614137228 1102 2 -0.11637848107215376 1102 3 -0.30425401144033515 1102 4 -0.18446510888938406 1102 5 -0.08560133190936625 1102 6 -0.0682806646012537 1102 7 -0.18110509815498688 1102 8 -0.1592167551360788 1102 9 -0.04316189348113397 1102 10-11 -0.1914610981835594 1102 12-13 -0.20546613051572393 1102 14-15 -0.3127571971525427 1102 16-17 -0.12986191308049655 1102 18-19 -0.12254219348604067 1102 20-21 -0.09971975713289538 1102 22-23 0.018632463935837507 1102 24-25 0.08198202274074617 1102 26-27 -0.06989321846989327 1102 28-29 0.20183563715721675 1102 30-31 0.03881931279023121 1102 32-33 0.04848225908575188 1102 34-35 -0.06981404105246725 1102 36-37 -0.016209868440490993 1102 38-39 -0.4380307082391397 1102 40-41 -0.26064892424030717 1102 42-43 -0.5918010895485608 1102 44-45 -0.3735657106445558 1102 46-47 -0.6418631026002615 1102 48-49 -0.7507670429460589 1102 50-51 -0.837941769684484 1102 52-53 -0.7647598762898511 1102 54-55 -0.8505107109482211 1102 56-57 -1.173628915442933 1102 58-59 -1.0749655989900972 1102 60-61 -1.004758701415767 1102 62-63 -1.3298466410428809 1102 64-65 -1.217536221073793 1102 66-67 -1.5423832669894537 1102 68-69 -1.9859416032112485 1102 70-71 -1.863122445006006 1102 72-73 -1.6778822143097756 1102 74-75 -1.9091804862477773 1102 76-77 -1.8569964025620393 1102 78-79 -1.4822035714723043 1102 80-81 -1.2472325794280543 1102 82-83 -1.2768245250967816 1102 84-85 -1.2106627402731789 1102 86-87 -0.861096529020152 1102 88-89 -0.8052229965308584 1102 90-91 -0.8869761501596578 1102 92-93 -1.0549544331198355 1102 94-95 -1.226138439225334 1102 96-97 -1.4128065756022394 1102 98-99 -1.2758497777069202 1102 100-101 -1.3560522697246267 1103 1 -0.025466560617207534 1103 2 0.22239867238755906 1103 3 0.18938319558236927 1103 4 0.22102072142446616 1103 5 0.10759301772041852 1103 6 -0.0437222091758116 1103 7 0.10419658750378602 1103 8 0.12636499843916482 1103 9 0.11941753202955141 1103 10-11 0.06067004361833739 1103 12-13 -0.06321788531625572 1103 14-15 0.07350022027117831 1103 16-17 0.09913736924546157 1103 18-19 0.07048586581834115 1103 20-21 0.04917134896032849 1103 22-23 0.20610292552277798 1103 24-25 0.11139790609246347 1103 26-27 0.0027581494868300638 1103 28-29 0.026093019572449805 1103 30-31 -0.027189194272729367 1103 32-33 0.11119121600780346 1103 34-35 -0.15780787955830533 1103 36-37 0.14653584739559733 1103 38-39 0.2714075805458904 1103 40-41 0.386789329536235 1103 42-43 0.5680301695317667 1103 44-45 0.4223610515952885 1103 46-47 0.33531136220745594 1103 48-49 0.15340596900934855 1103 50-51 0.49021030719416814 1103 52-53 0.3701015776127221 1103 54-55 0.3024063714442633 1103 56-57 0.17646473226663772 1103 58-59 0.11299498145066167 1103 60-61 0.05939435570059004 1103 62-63 -0.2668663683267454 1103 64-65 -0.21376405632384277 1103 66-67 -0.35555171033696453 1103 68-69 -0.2844764979232153 1103 70-71 -0.7445574429930133 1103 72-73 -0.7332568926692744 1103 74-75 -0.45581179433999 1103 76-77 -0.18007224845466752 1103 78-79 0.25635302850319164 1103 80-81 0.007620472437025683 1103 82-83 -0.45417753320124277 1103 84-85 -0.3237051530582775 1103 86-87 -0.5565401741040858 1103 88-89 -0.6023925601500331 1103 90-91 -0.8789504539283222 1103 92-93 -0.8338408130669741 1103 94-95 -0.8430230337395024 1103 96-97 -1.1683160366465408 1103 98-99 -2.3642024532307353 1103 100-101 -2.9169320330672353 1104 1 0.1560814997761497 1104 2 0.024263858329458543 1104 3 0.24916690870842118 1104 4 0.043141519126486116 1104 5 0.17080392763018182 1104 6 0.26037559774778885 1104 7 0.2685812024659171 1104 8 0.23516751171680284 1104 9 0.298702763777257 1104 10-11 0.35734500366183397 1104 12-13 0.31934020951383957 1104 14-15 0.09072408103032359 1104 16-17 0.324206434949879 1104 18-19 0.24175508201184925 1104 20-21 0.208011484510358 1104 22-23 0.2893964396627027 1104 24-25 0.2669563908760324 1104 26-27 0.27007949861602754 1104 28-29 0.21612668738701046 1104 30-31 0.11307322794253594 1104 32-33 0.2079600944246991 1104 34-35 -0.02079140814299052 1104 36-37 -0.25326807740379564 1104 38-39 -0.38237139821537625 1104 40-41 -0.07950715593936764 1104 42-43 0.1704423888064568 1104 44-45 0.03227575613686895 1104 46-47 -0.2186450179257875 1104 48-49 -0.09350323773740143 1104 50-51 -0.1463063646901901 1104 52-53 0.07567296930726286 1104 54-55 -0.0014030895960956968 1104 56-57 -0.3527488249329451 1104 58-59 -0.3863074961270101 1104 60-61 -0.1430354389422206 1104 62-63 -0.2730397163794507 1104 64-65 -0.5242741264605435 1104 66-67 -0.45264738075487543 1104 68-69 -0.4234344982857081 1104 70-71 -0.3300094320303799 1104 72-73 -0.3788852944641583 1104 74-75 -0.3364558217355942 1104 76-77 -0.6698675503390774 1104 78-79 -0.7279582092970358 1104 80-81 -0.8722738556621472 1104 82-83 -1.0927785338987661 1104 84-85 -0.867558442672884 1104 86-87 -1.5192941244426876 1104 88-89 -1.172960409309436 1104 90-91 -1.2367739642785658 1104 92-93 -1.4755037724294269 1104 94-95 -1.4812066194441407 1104 96-97 -1.8340352811254057 1104 98-99 -2.2411858609847677 1104 100-101 -2.4265710240214133 1105 1 -0.048653425153709406 1105 2 -0.25197331646212007 1105 3 -0.07977134591712698 1105 4 -0.15781622931754669 1105 5 -0.04237233894479431 1105 6 0.0011207678951379307 1105 7 0.11127027307121296 1105 8 0.0013911823549861424 1105 9 -0.0010445939432770501 1105 10-11 -0.09158338446049186 1105 12-13 -0.12220823352205201 1105 14-15 -0.33816733262033694 1105 16-17 -0.2848557987666567 1105 18-19 -0.20865079005249498 1105 20-21 -0.08573624195827989 1105 22-23 -0.1619462518994439 1105 24-25 -0.11236581025113423 1105 26-27 -0.008432453307193555 1105 28-29 -0.00703567638797864 1105 30-31 0.2657000598482142 1105 32-33 0.21483923972640895 1105 34-35 -0.20779901730986694 1105 36-37 0.03052095881822936 1105 38-39 -0.0693217019342498 1105 40-41 -0.020213458043720323 1105 42-43 -0.18704145354874413 1105 44-45 0.24397204722861687 1105 46-47 0.540891356084451 1105 48-49 0.41879002663007014 1105 50-51 0.4595791539739409 1105 52-53 0.4470097808321398 1105 54-55 0.4804086595156747 1105 56-57 0.022108534332346608 1105 58-59 -0.14121458655501584 1105 60-61 -0.023967175695943865 1105 62-63 0.05468017459071106 1105 64-65 -0.18950566538589086 1105 66-67 -0.007563440731257032 1105 68-69 -0.09811146996684883 1105 70-71 -0.06374340519149513 1105 72-73 -0.28261961644104616 1105 74-75 0.035615994448463084 1105 76-77 -0.20532965648869705 1105 78-79 -0.37465517310490526 1105 80-81 -0.46460040702028493 1105 82-83 -0.09061587612191957 1105 84-85 0.028327524468668486 1105 86-87 -0.04439889375088768 1105 88-89 -0.23277234777833655 1105 90-91 -0.11168115436821324 1105 92-93 -0.6052109166747144 1105 94-95 -0.5854377547398109 1105 96-97 -1.08811268832795 1105 98-99 -1.3555899590717608 1105 100-101 -1.7299385911310754 1106 1 0.2878557189912101 1106 2 0.25353968361280366 1106 3 0.2701960890536803 1106 4 0.42748397414183614 1106 5 0.36770495256696734 1106 6 0.28819446860811126 1106 7 0.3771219494497515 1106 8 0.2623321676056207 1106 9 0.5799385817522591 1106 10-11 0.5406018154542025 1106 12-13 0.5925221107450582 1106 14-15 0.7257811627600432 1106 16-17 0.19478385333508896 1106 18-19 0.5477685553039677 1106 20-21 0.6424927391885831 1106 22-23 0.6085855667277613 1106 24-25 0.7826162933921097 1106 26-27 0.8405637945097979 1106 28-29 0.7515191279624815 1106 30-31 0.7701496532176293 1106 32-33 0.8680187969572799 1106 34-35 0.6326986224459787 1106 36-37 0.4547251329056863 1106 38-39 0.5722785378365884 1106 40-41 0.7529725545557682 1106 42-43 0.6381736773741977 1106 44-45 0.5782535128278425 1106 46-47 0.6105459362261172 1106 48-49 0.5411884266208489 1106 50-51 0.7563128527272553 1106 52-53 0.9417475026633824 1106 54-55 1.0062618344619239 1106 56-57 0.7337725785055156 1106 58-59 1.0519104458659214 1106 60-61 1.4741250323283168 1106 62-63 1.2432979764224221 1106 64-65 1.1913899017355298 1106 66-67 0.9684018386075302 1106 68-69 1.201296595785692 1106 70-71 1.1172201738493044 1106 72-73 1.3609316432203897 1106 74-75 0.9277969292088919 1106 76-77 0.5374066128846131 1106 78-79 0.8640061448277621 1106 80-81 1.0440278847854287 1106 82-83 1.0968347720086165 1106 84-85 1.01359545108496 1106 86-87 1.3951569538332507 1106 88-89 0.7776109973706298 1106 90-91 0.9394603507701476 1106 92-93 0.8209558047473102 1106 94-95 0.6031281735940688 1106 96-97 0.5761015146357558 1106 98-99 0.2860996183897804 1106 100-101 0.02787557494480808 1107 1 -0.1408682266652903 1107 2 -0.20961914278007754 1107 3 -0.1318207715915385 1107 4 -0.1085495701339525 1107 5 -0.06447077872479667 1107 6 -0.12624107593320133 1107 7 -0.1847225431820192 1107 8 -0.13540868294975894 1107 9 -0.19680169350552035 1107 10-11 -0.21279741895744309 1107 12-13 -0.0073805311985637445 1107 14-15 -0.009031533615576848 1107 16-17 -0.17754156596836168 1107 18-19 -0.18218176996045088 1107 20-21 -0.19070967706136344 1107 22-23 -0.2835461321703221 1107 24-25 -0.11061898120186697 1107 26-27 -0.15985938113298914 1107 28-29 -0.33995042551806876 1107 30-31 -0.5102194223233667 1107 32-33 -0.4321095386624947 1107 34-35 -0.30812593391105736 1107 36-37 -0.35885355010533004 1107 38-39 -0.511601512612593 1107 40-41 -0.19879925143617783 1107 42-43 0.1846870419801192 1107 44-45 0.08249909852506221 1107 46-47 -0.09279134305874237 1107 48-49 -0.4853471754502223 1107 50-51 -0.27151440310316843 1107 52-53 -0.3269220109888735 1107 54-55 -0.2723647531381914 1107 56-57 -0.4011596027574882 1107 58-59 -0.6363529298034152 1107 60-61 -0.32297192744510994 1107 62-63 -0.2940659435669595 1107 64-65 -0.24040690757543715 1107 66-67 -0.5490313383628624 1107 68-69 -0.39858945103468457 1107 70-71 -0.4654041789408865 1107 72-73 -0.355761944423989 1107 74-75 -0.5320262774114575 1107 76-77 -0.4402840097644116 1107 78-79 -0.3413391443699183 1107 80-81 -0.6093276491350004 1107 82-83 -0.45370844169231717 1107 84-85 -0.7726953176011477 1107 86-87 -1.0978537334261205 1107 88-89 -1.2623141033064513 1107 90-91 -1.5285442516106968 1107 92-93 -1.1080170677578884 1107 94-95 -0.821944844948213 1107 96-97 -0.9375977596041913 1107 98-99 -1.0541999789018952 1107 100-101 -0.8263252039196427 1108 1 0.025456344320296864 1108 2 -0.13065230110137094 1108 3 -0.09934517343797467 1108 4 -0.16294718809088948 1108 5 -0.19294165507702843 1108 6 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0.32608339270635156 2316 80-81 0.26115368031415187 2316 82-83 0.23741642589243028 2316 84-85 0.03990399383953047 2316 86-87 -0.37880579910408585 2316 88-89 -0.13790997195052057 2316 90-91 0.27192251216048646 2316 92-93 0.12786415575905963 2316 94-95 0.11997451416195659 2316 96-97 0.2269612975991997 2316 98-99 0.45794912067437465 2316 100-101 0.09122974841086595 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5068.0 3 753.0 4 591.0 5 503.0 6 493.0 7 602.0 8 645.0 9 815.0 10 1017.0 11 1455.0 12 2269.0 13 3216.0 14 3916.0 15 5410.0 16 6375.0 17 8298.0 18 7262.0 19 4907.0 20 4691.0 21 4630.0 22 4619.0 23 4805.0 24 5370.0 25 5678.0 26 6340.0 27 7364.0 28 8506.0 29 9828.0 30 11604.0 31 14332.0 32 17476.0 33 22317.0 34 31918.0 35 51583.0 36 54008.0 37 73924.0 38 49542.0 39 3634.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.108739609076615 24.11592900471804 16.04381037968996 17.73152100651539 2 14.452228055566154 25.43415267000806 40.19657795444904 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35.85597182067128 15.89748601030217 22-23 17.148576187484476 31.172261252766198 35.7373176332018 15.941844926547526 24-25 16.859944871869814 32.385303362096494 35.4823261732714 15.272425592762298 26-27 15.86390587051129 32.17906980401673 36.58672759830057 15.37029672717142 28-29 15.410723892507248 32.48873779014721 36.46193086671002 15.638607450635526 30-31 15.982754361933846 32.12404284463207 35.873060427099006 16.020142366335076 32-33 15.708718455542952 32.87721386776878 35.76453543873047 15.649532237957795 34-35 16.345419761624616 32.23580211430163 35.6992733772241 15.719504746849655 36-37 16.42938132177779 31.63128529249975 35.669027919665005 16.270305466057454 38-39 15.58949485336697 32.876054352754224 35.5339884273703 16.000462366508504 40-41 15.525467252221365 32.26080154252436 36.31913105059781 15.894600154656475 42-43 15.456679992906638 32.221815675878304 35.01594883801323 17.305555493201823 44-45 15.838015501370705 31.678948590973206 35.23055118852445 17.252484719131637 46-47 16.646185373101833 32.07269568613456 34.765789393944495 16.515329546819117 48-49 16.212622273292943 31.724007141422383 34.618841729516944 17.44452885576773 50-51 15.294212709414449 31.943334650123 35.04459876820312 17.717853872259433 52-53 15.807366201908026 32.598141788223096 34.27201339187041 17.322478617998467 54-55 16.063901420779743 31.657351917940428 34.308464076493166 17.97028258478666 56-57 16.408234523243184 31.71186798194042 33.87787342135734 18.002024073459054 58-59 16.645418898991554 30.874989058147804 34.33676664339886 18.142825399461774 60-61 16.542132105006253 31.78947132189164 34.092247961983205 17.576148611118903 62-63 16.014329130061995 31.47807310908903 34.76598447677577 17.741613284073207 64-65 16.120989053531652 31.547822769327443 33.97467331721194 18.356514859928968 66-67 16.27835735229266 31.315650980563774 33.32009232795951 19.085899339184063 68-69 16.131371294519088 30.731274764005345 34.02565915749066 19.111694783984916 70-71 17.169875057376196 30.725246310808945 33.76525870069996 18.3396199311149 72-73 17.13438885995942 29.93975687274425 34.06070998904671 18.865144278249627 74-75 16.45356979343406 31.353855269182084 33.064577666460195 19.127997270923665 76-77 16.83444907795403 30.40255437642273 33.5388681895543 19.224128356068945 78-79 17.104609195054117 30.101410593418578 33.2965342685681 19.497445942959203 80-81 17.771676751311862 30.184036827560984 32.42831449731793 19.615971923809226 82-83 17.857403308160677 29.697714939005575 33.09720176234782 19.347679990485926 84-85 17.600116678914212 29.71497809465577 32.75020054188381 19.934704684546215 86-87 17.80487339691629 29.928679533595336 32.737855494435834 19.528591575052534 88-89 18.43189282258843 29.42058558770908 32.34302428687198 19.804497302830512 90-91 18.698030302860165 28.760093560894866 32.59449571778117 19.94738041846379 92-93 19.593599432417747 29.135851530541224 32.38766227059843 18.882886766442596 94-95 19.328419696156722 28.06419458182822 32.759240010147565 19.84814571186749 96-97 19.437285898362614 28.086992048775887 32.60361060103352 19.872111451827973 98-99 19.620170768277685 28.370586017112743 31.876632937986514 20.132610276623062 100-101 20.4996220600144 26.900454089557734 32.45005071022392 20.14987314020394 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7581.0 1 4484.0 2 1629.0 3 1763.5 4 1299.0 5 1273.0 6 2451.0 7 4539.5 8 6164.0 9 6168.5 10 4475.0 11 2786.0 12 2370.5 13 2202.5 14 1925.0 15 1722.5 16 1573.0 17 1540.0 18 1573.5 19 1567.0 20 1660.5 21 1804.5 22 1974.5 23 2143.5 24 2307.5 25 2763.0 26 3833.5 27 4852.0 28 5082.5 29 5643.0 30 7303.5 31 8837.0 32 10934.5 33 14043.0 34 16836.5 35 19040.5 36 21839.0 37 23920.0 38 23083.0 39 21362.0 40 19738.0 41 18051.0 42 16120.5 43 15309.5 44 14951.0 45 14025.5 46 12627.5 47 12153.5 48 14117.0 49 13349.0 50 9546.0 51 6365.5 52 4478.0 53 3576.5 54 2908.0 55 2353.0 56 1916.0 57 1567.0 58 1265.5 59 1064.5 60 888.0 61 761.0 62 657.5 63 500.0 64 390.5 65 346.0 66 334.0 67 296.0 68 254.0 69 222.0 70 185.0 71 144.0 72 135.0 73 137.0 74 100.0 75 86.0 76 81.0 77 56.5 78 41.0 79 36.0 80 28.0 81 19.0 82 9.5 83 4.5 84 2.5 85 2.0 86 1.5 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14895774445670804 2 0.05406448255130517 3 0.16062310998645024 4 0.13011369244712448 5 0.1992085498155975 6 0.20167622329304294 7 0.17139113970621225 8 0.06931919132096805 9 0.12630001525470877 10-11 0.10229628233773926 12-13 0.15579993000780684 14-15 0.1424520598343518 16-17 0.1408817221668865 18-19 0.13314220080580755 20-21 0.13796538078445095 22-23 0.19842338098186485 24-25 0.22029594135013147 26-27 0.15142541793415348 28-29 0.130225859423372 30-31 0.09736093538284833 32-33 0.1254026794447286 34-35 0.09332292423793756 36-37 0.085022567995621 38-39 0.05159680907385971 40-41 0.0584389946249585 42-43 0.062140504841126695 44-45 0.04273561795030554 46-47 0.05283064581258245 48-49 0.04363295376028571 50-51 0.052942812788829965 52-53 0.027032241275652586 54-55 0.052494144883839876 56-57 0.028714745919365403 58-59 0.050923807216374586 60-61 0.03847327285289974 62-63 0.053391480693820055 64-65 0.041838282140325374 66-67 0.040043610520365036 68-69 0.06931919132096805 70-71 0.053727981622562614 72-73 0.052942812788829965 74-75 0.043072118879048105 76-77 0.021872560368266614 78-79 0.022545562225751742 80-81 0.026807907323157545 82-83 0.024340233845712083 84-85 0.021648226415771573 86-87 0.0223212282732567 88-89 0.06662718389102754 90-91 0.1104844716038083 92-93 0.08210622661318545 94-95 0.07560054199082923 96-97 0.06685151784352258 98-99 0.055634820218770475 100-101 0.1317961970908373 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 445764.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.074263704505263 #Duplication Level Percentage of deduplicated Percentage of total 1 76.27192790665457 21.412782173024347 2 9.595609807146175 5.387793602627173 3 3.7599963787235238 3.166773895928072 4 2.0944200267591198 2.3519720055693005 5 1.289057088344296 1.8094664314169753 6 0.8482951970877126 1.4289157837383426 7 0.6518460448550824 1.2810068430600245 8 0.5153855914037027 1.157525680205596 9 0.44995897245200406 1.1369040163943205 >10 3.6028465842596544 21.23644447774397 >50 0.5887733318092406 11.526441309768181 >100 0.3111403785983792 15.310056199026754 >500 0.011169141795839253 2.3181444446709416 >1k 0.006382366740479574 3.4008410867520364 >5k 0.003191183370239787 7.074932050073982 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9593 2.15203560628494 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8645 1.9393670193196402 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7687 1.7244550928293894 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5663 1.270403172979424 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2915 0.653933471523048 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2663 0.5974013154942974 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2233 0.5009377159214292 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1720 0.3858543982914726 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1708 0.38316239086153214 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1424 0.3194515483529401 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1268 0.28445545176371356 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1253 0.2810904424762879 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 952 0.21356592277528022 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 938 0.2104252474403496 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 810 0.1817105015209842 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 808 0.1812618336159941 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 744 0.16690446065631143 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 725 0.1626421155589056 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 718 0.1610717778914403 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 709 0.15905277231898493 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 699 0.1568094327940345 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 688 0.15434175931658903 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 687 0.154117425364094 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 649 0.1455927351692824 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 626 0.14043305426189645 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 597 0.13392736963954022 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 492 0.11037230462756077 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 482 0.10812896510261034 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 469 0.1052126237201748 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 467 0.10476395581518472 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 461 0.10341795210021447 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.121669762475211E-4 0.0 14-15 0.0 0.0 0.0 5.608348812376055E-4 0.0 16-17 0.0 0.0 0.0 0.00100950278622769 0.0 18-19 0.0 0.0 0.0 0.0014581706912177744 0.0 20-21 0.0 0.0 0.0 0.0023555065011979432 0.0 22-23 0.0 0.0 0.0 0.005944849741118618 0.0 24-25 0.0 0.0 0.0 0.014918207840920308 0.0 26-27 0.0 0.0 0.0 0.0361177663517018 0.0 28-29 0.0 0.0 0.0 0.06864618946348292 0.0 30-31 0.0 0.0 0.0 0.14951857933794566 0.0 32-33 0.0 0.0 0.0 0.2695372439227932 0.0 34-35 0.0 0.0 0.0 0.4352078678403819 0.0 36-37 0.0 0.0 0.0 0.6629068296228497 0.0 38-39 0.0 0.0 0.0 0.9663185003723944 0.0 40-41 0.0 0.0 0.0 1.261317647903375 0.0 42-43 0.0 0.0 0.0 1.534444235066089 0.0 44-45 0.0 0.0 0.0 1.861859638732603 0.0 46-47 0.0 0.0 0.0 2.2248319738695814 0.0 48-49 0.0 0.0 0.0 2.5697454258307086 0.0 50-51 0.0 0.0 0.0 2.9100600317656875 0.0 52-53 0.0 0.0 0.0 3.231867086619826 0.0 54-55 0.0 0.0 0.0 3.581042883678359 0.0 56-57 0.0 0.0 0.0 3.9888820093143456 0.0 58-59 0.0 0.0 0.0 4.389093780565501 0.0 60-61 0.0 0.0 0.0 4.796259904344003 0.0 62-63 0.0 0.0 0.0 5.170448937105733 0.0 64-65 0.0 0.0 0.0 5.573016214858086 0.0 66-67 0.0 0.0 0.0 6.021347618919428 0.0 68-69 0.0 0.0 0.0 6.500749275401334 0.0 70-71 0.0 0.0 0.0 7.012006353137535 0.0 72-73 0.0 0.0 0.0 7.478508807350975 0.0 74-75 0.0 0.0 0.0 7.989205050205938 0.0 76-77 0.0 0.0 0.0 8.50865031720821 0.0 78-79 0.0 0.0 0.0 9.104481295035042 0.0 80-81 0.0 0.0 0.0 9.70861262910419 0.0 82-83 0.0 0.0 0.0 10.274943692177924 0.0 84-85 0.0 0.0 0.0 10.83757324503549 0.0 86-87 0.0 0.0 0.0 11.409624823897847 0.0 88-89 0.0 0.0 0.0 12.018915838874381 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTGCA 25 0.0050012446 57.0518 7 CCCTATA 100 0.0 52.315125 2 GCTACAC 55 4.9188013E-5 43.240505 3 GTAAGCG 80 0.0 41.555893 94-95 CGGATTA 80 1.070271E-5 35.653366 9 GTAAGGT 100 1.2831297E-6 33.295185 4 TACACAG 90 2.3873967E-5 31.709702 5 TGTAAGG 120 1.4844954E-7 31.7097 3 GTCGGGG 30 0.0039579035 31.654524 92-93 TGGGGGA 580 0.0 31.148972 6 CCCCTAT 190 1.0913936E-11 30.061052 1 TATATCG 80 4.4536963E-4 29.727842 4 ATCGGAT 80 4.4654452E-4 29.714478 7 ATGGGGG 725 0.0 29.522827 5 TAAGGTG 100 4.900792E-5 28.53873 5 ACTATAT 200 2.3646862E-11 28.5259 8 GGGGGAA 600 0.0 27.733513 7 TAGGTCG 105 6.833009E-5 27.179745 5 CATGGGG 1770 0.0 27.141354 4 GTCGGCG 35 0.008397086 27.138542 90-91 >>END_MODULE