##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938248_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 445764 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.462058398614516 33.0 31.0 34.0 31.0 34.0 2 32.66140379214113 34.0 31.0 34.0 31.0 34.0 3 32.68185856192963 34.0 31.0 34.0 31.0 34.0 4 36.17136197629239 37.0 35.0 37.0 35.0 37.0 5 36.172492619412964 37.0 35.0 37.0 35.0 37.0 6 36.24063630082286 37.0 37.0 37.0 35.0 37.0 7 36.19277016537899 37.0 37.0 37.0 35.0 37.0 8 36.21180490124819 37.0 37.0 37.0 35.0 37.0 9 37.85153130355973 39.0 38.0 39.0 35.0 39.0 10-11 37.85717554580451 39.0 38.0 39.0 35.0 39.0 12-13 37.79910333719188 39.0 38.0 39.0 35.0 39.0 14-15 38.963519934315016 40.0 38.0 41.0 35.5 41.0 16-17 38.877115469172026 40.0 38.0 41.0 35.5 41.0 18-19 39.139292316113455 40.5 38.5 41.0 35.5 41.0 20-21 39.15532434202852 41.0 39.0 41.0 35.0 41.0 22-23 39.022130544413635 40.5 38.5 41.0 35.0 41.0 24-25 38.89031527893684 40.0 38.0 41.0 35.0 41.0 26-27 38.63321623101013 40.0 38.0 41.0 35.0 41.0 28-29 38.46699823224846 40.0 38.0 41.0 35.0 41.0 30-31 38.08532093215244 40.0 37.0 41.0 34.0 41.0 32-33 37.68752523756966 40.0 35.0 41.0 33.5 41.0 34-35 37.399379492287395 40.0 35.0 41.0 33.0 41.0 36-37 37.22258639100511 40.0 35.0 41.0 33.0 41.0 38-39 36.98108864780467 40.0 35.0 41.0 33.0 41.0 40-41 36.77387810590358 40.0 35.0 41.0 33.0 41.0 42-43 36.43713153148303 39.5 35.0 41.0 31.5 41.0 44-45 36.017031433673424 39.0 35.0 41.0 30.5 41.0 46-47 35.46366014303533 39.0 35.0 41.0 28.0 41.0 48-49 34.96108815426997 39.0 35.0 41.0 24.5 41.0 50-51 34.55221933579203 39.0 35.0 40.5 23.0 41.0 52-53 34.13769730171122 38.0 35.0 40.0 20.0 41.0 54-55 33.845898278012584 38.0 34.5 40.0 16.0 41.0 56-57 33.503501852998454 38.0 34.0 40.0 8.5 41.0 58-59 33.079527507829255 38.0 33.0 40.0 2.0 41.0 60-61 32.5886130329053 37.0 33.0 40.0 2.0 41.0 62-63 32.15213655656356 37.0 33.0 40.0 2.0 41.0 64-65 31.691629427230552 36.0 32.0 39.0 2.0 41.0 66-67 31.112666119291823 36.0 31.0 39.0 2.0 40.0 68-69 30.562522994230132 35.0 31.0 38.5 2.0 40.0 70-71 30.054593910679195 35.0 29.5 38.0 2.0 40.0 72-73 29.522247198068932 35.0 29.0 37.0 2.0 39.5 74-75 28.983339839017958 34.5 28.5 37.0 2.0 39.0 76-77 27.59129382363762 32.5 26.5 35.0 2.0 37.5 78-79 28.246630504033526 34.0 28.0 36.0 2.0 38.0 80-81 27.92660690410172 34.0 27.5 35.0 2.0 37.0 82-83 27.420410800333812 34.0 26.0 35.0 2.0 37.0 84-85 26.89245542484364 33.5 25.5 35.0 2.0 36.0 86-87 26.40776060875261 33.0 24.5 35.0 2.0 36.0 88-89 25.780836945109968 33.0 22.0 35.0 2.0 35.0 90-91 25.35578131028975 32.5 20.0 35.0 2.0 35.0 92-93 24.68650900476485 32.0 14.5 34.5 2.0 35.0 94-95 24.038933157455517 31.5 3.5 34.0 2.0 35.0 96-97 23.196553557487817 31.0 2.0 34.0 2.0 35.0 98-99 22.04509000278174 31.0 2.0 34.0 2.0 35.0 100-101 17.979324261268296 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2049493798595634 1101 2 0.09427301714001146 1101 3 0.08870801359228153 1101 4 0.07598952068955356 1101 5 -7.198869012370324E-4 1101 6 0.14156945420630507 1101 7 0.13359277408497405 1101 8 0.17316494277937267 1101 9 0.08690538203207865 1101 10-11 0.06913122546215078 1101 12-13 0.24694423118123154 1101 14-15 0.06109253620312671 1101 16-17 0.2580471007810772 1101 18-19 0.17679294750946895 1101 20-21 0.19316261969924398 1101 22-23 0.17210695128770936 1101 24-25 -0.07293206639401717 1101 26-27 -0.1200352336843622 1101 28-29 -0.14583113372351875 1101 30-31 0.08210753486647349 1101 32-33 0.17119648961531198 1101 34-35 -0.006970119884321946 1101 36-37 0.1908324285935734 1101 38-39 0.25080484935500635 1101 40-41 -0.2105490157205523 1101 42-43 -0.5009457890605375 1101 44-45 -0.3699463653353874 1101 46-47 -0.35383846093282045 1101 48-49 -0.10096410190107719 1101 50-51 -0.08863943064569213 1101 52-53 -0.2842325340124958 1101 54-55 -0.1770755214697104 1101 56-57 -0.45780704917389414 1101 58-59 0.1052587261104847 1101 60-61 0.12541666343660296 1101 62-63 0.39468836123738527 1101 64-65 0.3113041292647942 1101 66-67 0.09619445074760336 1101 68-69 -0.022163142480113862 1101 70-71 -0.040334786116122956 1101 72-73 -0.2865868699817504 1101 74-75 -0.16772655299618222 1101 76-77 -0.04939220389847421 1101 78-79 -0.36376130935602546 1101 80-81 -0.4059249555284694 1101 82-83 -0.21514882590345152 1101 84-85 -0.06710716379802051 1101 86-87 -0.26363528195151176 1101 88-89 -0.7607346835643831 1101 90-91 -0.8339314244612162 1101 92-93 -0.7771212012408455 1101 94-95 -0.5557366813617399 1101 96-97 -0.4190259201042892 1101 98-99 -0.17080979872675428 1101 100-101 -0.37173429654589185 1102 1 0.00941397145160039 1102 2 0.09558829145117187 1102 3 -0.10289380699206419 1102 4 -0.047370974587693127 1102 5 -0.0793365853791883 1102 6 -0.09362606284943809 1102 7 -0.1677824522645608 1102 8 -0.013916004707539287 1102 9 0.2023681138874096 1102 10-11 0.08456275778731737 1102 12-13 0.008848993085997847 1102 14-15 -0.01623029940594023 1102 16-17 0.1344266094190658 1102 18-19 0.04374224521416181 1102 20-21 -0.014354020050184602 1102 22-23 0.049306212287071105 1102 24-25 0.15446968567371755 1102 26-27 -0.053601645860744895 1102 28-29 -0.28332012212463553 1102 30-31 -0.44877694122612155 1102 32-33 -0.5733374750496836 1102 34-35 -0.5032751167032004 1102 36-37 -0.5388756416848892 1102 38-39 -0.8589318510805626 1102 40-41 -0.7537603650084748 1102 42-43 -0.6405208636947108 1102 44-45 -1.1343899125384453 1102 46-47 -1.1495855367415615 1102 48-49 -0.7486051727178946 1102 50-51 -0.6440247142555435 1102 52-53 -0.5420568867751783 1102 54-55 -0.9582261112020092 1102 56-57 -1.1748793097328019 1102 58-59 -1.2384193926190221 1102 60-61 -1.2612984542651517 1102 62-63 -1.1605409247216016 1102 64-65 -0.85383008989049 1102 66-67 -1.304144461795829 1102 68-69 -1.2168879748591088 1102 70-71 -0.9428270779969061 1102 72-73 -0.7828490439160696 1102 74-75 -0.8827463738741734 1102 76-77 -0.8694045613594454 1102 78-79 -0.6129629684369284 1102 80-81 -0.7272923514076837 1102 82-83 -0.7903733401695909 1102 84-85 -0.733452658830565 1102 86-87 -0.9021061379198017 1102 88-89 -0.7738996617858867 1102 90-91 -0.9928930306864352 1102 92-93 -1.0568586357830085 1102 94-95 -0.621121108513897 1102 96-97 -0.8864071171874421 1102 98-99 -1.0096825780543135 1102 100-101 -1.3574620408810354 1103 1 0.10439200362439749 1103 2 0.07046970019279541 1103 3 0.01785047349595459 1103 4 0.14437052272540285 1103 5 0.06342803504126238 1103 6 0.08157392314716816 1103 7 0.08349098154314305 1103 8 0.21061094307729888 1103 9 0.05983104864908739 1103 10-11 0.1557262649377904 1103 12-13 0.1974134699716359 1103 14-15 0.24614082559187267 1103 16-17 0.12525438515468323 1103 18-19 0.1574585714232697 1103 20-21 0.12322059179319211 1103 22-23 0.19683198728750284 1103 24-25 0.2005671143001635 1103 26-27 0.13153199904814272 1103 28-29 0.13953867113272622 1103 30-31 0.07186931549113496 1103 32-33 0.11718747725124956 1103 34-35 0.08804452831071785 1103 36-37 0.027622984231889802 1103 38-39 -0.10597800977404859 1103 40-41 -0.08503694958023544 1103 42-43 -0.06759817994390005 1103 44-45 -0.309721180370893 1103 46-47 -0.3438826537924413 1103 48-49 -0.3984590122739817 1103 50-51 -0.5523199758564772 1103 52-53 -0.481579721062495 1103 54-55 -0.2691977718297167 1103 56-57 -0.25443610225498503 1103 58-59 -0.12661102909085997 1103 60-61 -0.1827043952058247 1103 62-63 -0.21276483902082077 1103 64-65 -0.9579278335933417 1103 66-67 -0.6407773452700738 1103 68-69 -0.538397190843984 1103 70-71 -0.5586264093347566 1103 72-73 -0.7924151136879907 1103 74-75 -0.5721160460196657 1103 76-77 -0.4641812947172852 1103 78-79 -0.8297880485188465 1103 80-81 -0.5759681552901235 1103 82-83 -0.2998693929623961 1103 84-85 -0.2449616997729933 1103 86-87 -0.503499475804233 1103 88-89 -0.8437328463331362 1103 90-91 -1.1422479992663206 1103 92-93 -1.3174968405476655 1103 94-95 -1.1070301914665102 1103 96-97 -1.058009980881888 1103 98-99 -1.5557625024359787 1103 100-101 -1.736008838479453 1104 1 0.14901696174559476 1104 2 0.07467680393689591 1104 3 0.058026666561374896 1104 4 0.11179018407003127 1104 5 -0.03854754618165401 1104 6 -0.02672879059952038 1104 7 0.053019687062352716 1104 8 0.10674051747877655 1104 9 -0.020675121952812958 1104 10-11 -0.0427710410878106 1104 12-13 -0.14164009645500641 1104 14-15 -0.028155747760571614 1104 16-17 -0.16692237911020413 1104 18-19 0.14450497588740774 1104 20-21 -0.022300710102200583 1104 22-23 -0.1521461698253006 1104 24-25 -0.23589792994034298 1104 26-27 -0.07630222129204611 1104 28-29 -0.18312037221802058 1104 30-31 -0.324106474439283 1104 32-33 -0.22456006357029423 1104 34-35 -0.31359931783890005 1104 36-37 -0.4807884771050439 1104 38-39 -0.5331473319689977 1104 40-41 -0.44111116157213104 1104 42-43 -0.2591257540487888 1104 44-45 -0.0690814369712669 1104 46-47 -0.07687711205918646 1104 48-49 -0.25449752429938144 1104 50-51 -0.42993774455364786 1104 52-53 -0.23327546947110278 1104 54-55 -0.2249517825836378 1104 56-57 -0.010039623919368523 1104 58-59 0.019750595092837386 1104 60-61 -0.8921698293998048 1104 62-63 -0.973533871222191 1104 64-65 -0.5109450299925875 1104 66-67 -0.7822261048335442 1104 68-69 -0.7106248138303677 1104 70-71 -0.9753960566740112 1104 72-73 -0.9494318047954025 1104 74-75 -0.8193135986496536 1104 76-77 -0.7671531796205926 1104 78-79 -0.3857271498683055 1104 80-81 -0.18592679825201586 1104 82-83 -0.1878621211538345 1104 84-85 -0.8482060028821827 1104 86-87 -0.876715164017206 1104 88-89 -1.058610944678307 1104 90-91 -0.8758850569300023 1104 92-93 -0.7674756951828954 1104 94-95 -0.5547462174560884 1104 96-97 -1.0535447389184966 1104 98-99 -1.3436140784521875 1104 100-101 -1.6738085122366826 1105 1 -0.07494767830144866 1105 2 -0.0060840130892998445 1105 3 -0.07586377538820699 1105 4 -0.07128826459674542 1105 5 -0.035313525681253566 1105 6 -0.10917388948878681 1105 7 -0.12946079171220504 1105 8 0.03314038991186408 1105 9 -0.004041592784339798 1105 10-11 -0.00481739372062151 1105 12-13 -0.08607374422084746 1105 14-15 -0.15421527566333992 1105 16-17 -0.13904203288563366 1105 18-19 -0.20885038267028477 1105 20-21 0.09986453887751168 1105 22-23 -0.017578028664537726 1105 24-25 -0.15607393241194956 1105 26-27 -0.14861173050825727 1105 28-29 0.08804900773703395 1105 30-31 0.07128118445876197 1105 32-33 0.27691742037171707 1105 34-35 0.2026978532651995 1105 36-37 0.5969177103884107 1105 38-39 0.49253796644290304 1105 40-41 0.4690388790399922 1105 42-43 0.5309419660415031 1105 44-45 0.5021816249335629 1105 46-47 0.7535859657915083 1105 48-49 1.0649479582872416 1105 50-51 1.3004700210820204 1105 52-53 1.120996776254664 1105 54-55 1.1054739221487893 1105 56-57 1.279175989074517 1105 58-59 1.33063265051468 1105 60-61 1.0139488975222903 1105 62-63 0.8897552621699418 1105 64-65 0.8640618217457607 1105 66-67 0.7469299104944156 1105 68-69 0.9814381324669519 1105 70-71 0.991082747784855 1105 72-73 0.8409697818180426 1105 74-75 0.7573984638979958 1105 76-77 0.6619848966106048 1105 78-79 0.3587603151878298 1105 80-81 0.30655871343580543 1105 82-83 0.5769936933559059 1105 84-85 0.3700380585293246 1105 86-87 0.13881212085066963 1105 88-89 -0.08138612294808212 1105 90-91 0.05414748259829949 1105 92-93 0.09707914114776273 1105 94-95 0.32866211126789224 1105 96-97 -0.06373015941590765 1105 98-99 -0.3217508042680457 1105 100-101 -1.068286758590311 1106 1 0.23710022975566147 1106 2 0.2666034375588282 1106 3 0.1282987846265442 1106 4 0.10554272578360013 1106 5 0.14737916995387934 1106 6 0.030936428149928474 1106 7 0.11041378969748905 1106 8 0.0938035637630108 1106 9 0.11193602091948662 1106 10-11 0.11216887132989228 1106 12-13 0.14548955009208697 1106 14-15 0.384715784458038 1106 16-17 0.3623758427217183 1106 18-19 0.25656728544307583 1106 20-21 0.3587824577127776 1106 22-23 0.24274623833411368 1106 24-25 0.15454169073285584 1106 26-27 0.20708484411176897 1106 28-29 0.15228857107745597 1106 30-31 0.25771165379864414 1106 32-33 0.06782687806311571 1106 34-35 0.30442754623172874 1106 36-37 0.2876046884113421 1106 38-39 0.3080729262985713 1106 40-41 0.36492588703428197 1106 42-43 0.31292630024515944 1106 44-45 0.44869140308790634 1106 46-47 0.9017165025669414 1106 48-49 0.8597248734279574 1106 50-51 0.5942181118491092 1106 52-53 0.8178682279633165 1106 54-55 0.661217759289066 1106 56-57 0.632028166896859 1106 58-59 0.5981385151018017 1106 60-61 0.5116664636177788 1106 62-63 0.8230200072116958 1106 64-65 0.8160298446162066 1106 66-67 0.8685674991836905 1106 68-69 1.1222026325505219 1106 70-71 0.7076603649469106 1106 72-73 0.9518436197075992 1106 74-75 0.9926880044717201 1106 76-77 0.7148382271773492 1106 78-79 0.7643591947202779 1106 80-81 0.5684449490913401 1106 82-83 0.65318721608514 1106 84-85 0.5312003707040347 1106 86-87 0.36426875172462303 1106 88-89 0.13173614288630375 1106 90-91 0.048844858375680644 1106 92-93 -0.22272385483492485 1106 94-95 -0.2873933126826742 1106 96-97 -0.7593307104277294 1106 98-99 -0.3564714654822332 1106 100-101 -0.6576417404634078 1107 1 0.15596114049602505 1107 2 0.09587280959937061 1107 3 0.13389134116059864 1107 4 -0.018527947314595394 1107 5 -0.0734023497168721 1107 6 0.03723704327583022 1107 7 -0.1264815946455684 1107 8 -0.06420966808192219 1107 9 -0.13412540316335964 1107 10-11 0.01953765922209527 1107 12-13 -0.19948434024733785 1107 14-15 -0.01831337870520855 1107 16-17 -0.1687732036118632 1107 18-19 -0.0819842921999765 1107 20-21 -0.11194374488048453 1107 22-23 -0.01489581592087319 1107 24-25 -0.15715897749743846 1107 26-27 0.001678706128849683 1107 28-29 -0.08495254499989358 1107 30-31 -0.36304552635801457 1107 32-33 -0.1599605494715064 1107 34-35 -0.06516797529767615 1107 36-37 -0.09014733619368798 1107 38-39 -0.24100161899504968 1107 40-41 -0.5602549998091106 1107 42-43 -0.5474478644669674 1107 44-45 -0.41692405471622607 1107 46-47 -0.3384674820327902 1107 48-49 -0.4550154682103127 1107 50-51 -0.6380168087155695 1107 52-53 -0.7790223991110707 1107 54-55 -0.23929447684847815 1107 56-57 -0.09352998241499222 1107 58-59 -0.3983516163315741 1107 60-61 -0.579313533727003 1107 62-63 -0.5118751116093812 1107 64-65 -0.6458041377708348 1107 66-67 -0.6381535257311199 1107 68-69 -0.3601733465617478 1107 70-71 -0.4286519773994186 1107 72-73 -0.8397907786638648 1107 74-75 -0.23578258896989013 1107 76-77 -0.6250796822796545 1107 78-79 -0.505148372655924 1107 80-81 -0.5428585425225876 1107 82-83 -0.34646152047280765 1107 84-85 -0.36810595314426564 1107 86-87 -0.8023545832313452 1107 88-89 -0.6290977067397527 1107 90-91 -0.8581228807047303 1107 92-93 -0.5664890670312346 1107 94-95 -0.9645268923613948 1107 96-97 -1.310283270502076 1107 98-99 -1.8044789443511071 1107 100-101 -2.0449442584967734 1108 1 -0.10572884153294382 1108 2 -0.14324766680924483 1108 3 -0.17082781737211405 1108 4 -0.002869361740756915 1108 5 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-0.3692043729226171 2316 48-49 0.06228127437443476 2316 50-51 0.14288560124206384 2316 52-53 0.39240569940587733 2316 54-55 0.3355201417955911 2316 56-57 -0.008767667796348633 2316 58-59 0.408371768202322 2316 60-61 0.3657506876713015 2316 62-63 0.6010419964414666 2316 64-65 0.6733497287545909 2316 66-67 0.5406898270231153 2316 68-69 0.2716625909382522 2316 70-71 0.44027347281244644 2316 72-73 0.5319319437937615 2316 74-75 0.665899084758923 2316 76-77 0.7282704236525461 2316 78-79 0.8157268921745846 2316 80-81 0.6898778187062256 2316 82-83 0.4470203386373619 2316 84-85 0.7739921665591254 2316 86-87 1.197959902997468 2316 88-89 1.1190484797009255 2316 90-91 0.8997061712020518 2316 92-93 0.9111162030958866 2316 94-95 1.3570104487403007 2316 96-97 1.9784150875487718 2316 98-99 2.571596929589571 2316 100-101 2.815506615443905 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 3.0 5 14.0 6 61.0 7 115.0 8 231.0 9 427.0 10 742.0 11 1252.0 12 1896.0 13 2866.0 14 3557.0 15 4854.0 16 6151.0 17 8195.0 18 7741.0 19 5452.0 20 4566.0 21 4616.0 22 4952.0 23 5024.0 24 5597.0 25 5938.0 26 5853.0 27 6089.0 28 7097.0 29 8668.0 30 10137.0 31 12252.0 32 15543.0 33 19763.0 34 29587.0 35 50409.0 36 56692.0 37 87003.0 38 59213.0 39 3207.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.92474474056757 24.63884520206496 16.213881715774146 18.22252834159332 2 14.10359388111339 25.490125689361946 40.17033802854822 20.235942400976437 3 17.26506848546717 32.15216001256408 32.573281132562286 18.009490369406457 4 10.182293769797472 22.452912303371292 40.039348175267634 27.32544575156361 5 8.784587152670282 42.96479255560192 35.60474517563342 12.645875116094382 6 22.966773376525776 43.30476021688947 18.24626422019172 15.482202186393035 7 21.553108819913678 39.405604759469135 23.35024811335146 15.691038307265728 8 23.62842221444531 35.65788174908696 21.51048536893962 19.203210667528108 9 24.852388259258262 18.97551170574564 23.67912168770919 32.49297834728691 10-11 21.79224881327339 30.761905402858915 27.964573182221987 19.481272601645713 12-13 23.794338452288947 30.996620390051632 28.143525181066558 17.065515976592867 14-15 21.14022218034655 31.927768953975644 28.533708419701902 18.3983004459759 16-17 16.143114972737173 35.044368692562145 29.976433163172022 18.83608317152866 18-19 15.516506492224586 32.771713283261995 34.90569000637108 16.806090218142337 20-21 16.931941565492053 31.287071185649808 35.22727272727273 16.553714521585412 22-23 16.85578018861999 31.679655602516128 35.175115083317635 16.289449125546255 24-25 16.78605400360742 32.98175202132146 34.93128404389924 15.300909931171871 26-27 15.86335334043866 32.81101699669041 36.093790318056875 15.231839344814047 28-29 14.96886627473283 32.67521203575639 36.24692504686043 16.108996642650343 30-31 15.88564480433605 32.112623288985006 35.93437150519965 16.0673604014793 32-33 15.409947864789439 33.28913954469181 35.580374368499925 15.720538222018826 34-35 16.014618912151914 32.69399897694537 35.893288223205396 15.39809388769732 36-37 16.222446058659106 31.89134560595116 35.52097307531822 16.36523526007152 38-39 15.293101514301737 32.85586090858104 35.457767807066745 16.393269770050477 40-41 15.143969220760283 32.345683166762015 36.40452715056477 16.105820461912934 42-43 15.500280426247897 32.04879416713404 35.14649467190129 17.304430734716767 44-45 15.455729327322674 31.8299950534308 35.28204978648992 17.432225832756608 46-47 16.721217328490702 31.848987017765047 34.56040541722844 16.869390236515816 48-49 16.025385772436607 31.517302413959037 34.56927078718104 17.888041026423323 50-51 15.047426325424459 31.764869521519078 35.18084371298324 18.006860440073226 52-53 15.628274189459326 32.61333883752392 34.44744582373278 17.31094114928397 54-55 15.763053335098231 31.83986233787795 34.048940051017546 18.348144276006273 56-57 16.293270568384774 31.926768741978623 33.41178951903142 18.368171170605184 58-59 16.093643413635874 31.62664255196927 34.15139302768104 18.12832100671382 60-61 16.348264633201083 32.89799132456817 33.276686151053916 17.477057891176838 62-63 15.365948337761184 32.84391380053135 33.824585337832985 17.96555252387449 64-65 15.429622199933132 32.83037030554751 33.032318431805486 18.707689062713868 66-67 15.654819464980044 32.66884001229518 32.138441966960066 19.537898555764713 68-69 15.57132133732627 31.927615538333626 32.857554802797445 19.643508321542658 70-71 16.37051239046368 31.64002418745954 32.72632831896034 19.263135103116436 72-73 16.231865850197593 30.830830943116005 33.11669009210516 19.820613114581242 74-75 15.566505005222304 32.16569120083826 31.925386680488106 20.34241711345133 76-77 15.701822881606937 31.09949558864446 32.924284444923124 20.274397084825477 78-79 15.781478078132661 30.737849764215248 32.761121938248294 20.7195502194038 80-81 16.273377211236543 30.918104178422347 31.921455038237045 20.887063572104065 82-83 16.128681152834115 30.562559260319013 32.47751614405377 20.831243442793106 84-85 15.645121298578612 30.529567054446645 32.31468631109386 21.51062533588089 86-87 15.641087209224045 30.834818162641547 32.32118092177605 21.202913706358363 88-89 15.944544422354562 30.446826461784255 31.64753373693493 21.961095378926252 90-91 16.029315569857296 29.694356461082933 32.237926457503754 22.03840151155602 92-93 16.575039381033378 30.217257643959556 31.99714571655529 21.210557258451775 94-95 15.940770557146719 29.33257495952909 32.51662308261002 22.210031400714172 96-97 15.887028467255645 29.491179357733838 32.435843500210346 22.18594867480017 98-99 15.795112265330962 29.746077864967496 31.712614214479228 22.746195655222316 100-101 15.73533222574591 28.86035536280593 32.440906546469876 22.963405864978288 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7570.0 1 4386.5 2 1449.0 3 1639.0 4 1285.0 5 1315.0 6 2587.5 7 4608.5 8 6026.5 9 6059.0 10 4383.0 11 2699.5 12 2368.5 13 2204.0 14 1892.0 15 1636.0 16 1469.5 17 1422.0 18 1480.5 19 1507.5 20 1559.0 21 1753.5 22 1933.0 23 2032.0 24 2219.0 25 2644.0 26 3678.0 27 4790.0 28 5021.5 29 5619.5 30 7345.5 31 8866.5 32 11096.5 33 14125.5 34 16775.5 35 19127.0 36 22084.0 37 24219.5 38 23366.0 39 21540.0 40 20094.5 41 18516.5 42 16641.0 43 15984.5 44 15690.0 45 14933.5 46 13443.0 47 13135.5 48 15407.5 49 14095.0 50 9813.0 51 6416.0 52 3975.5 53 2783.5 54 2133.0 55 1785.0 56 1481.0 57 1109.0 58 878.0 59 744.5 60 599.5 61 466.0 62 356.0 63 218.0 64 140.0 65 119.0 66 105.0 67 109.0 68 84.5 69 66.5 70 61.5 71 56.0 72 57.5 73 53.0 74 41.5 75 47.0 76 37.0 77 16.5 78 20.5 79 26.0 80 15.5 81 6.5 82 2.0 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009197692052296732 2 0.013908705054692619 3 0.01099236367225707 4 0.0 5 0.0013460037149702533 6 0.0015703376674652955 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 5.608348812376055E-4 14-15 0.0 16-17 0.0023555065011979432 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0035893432399206754 26-27 0.019965721772058756 28-29 0.005720515788623577 30-31 0.0028041744061880276 32-33 0.0 34-35 0.008973358099801688 36-37 4.486679049900844E-4 38-39 0.003140675334930591 40-41 0.00201900557245538 42-43 0.003140675334930591 44-45 1.121669762475211E-4 46-47 2.243339524950422E-4 48-49 5.608348812376055E-4 50-51 0.004486679049900844 52-53 0.009646359957286814 54-55 0.007851688337326478 56-57 0.017946716199603377 58-59 0.0260227384894249 60-61 0.005047513931138449 62-63 0.02243339524950422 64-65 0.02355506501197943 66-67 0.013684371102197574 68-69 0.03219192218303856 70-71 0.01761021527086081 72-73 0.005271847883633492 74-75 0.017834549223355856 76-77 0.021536059439524052 78-79 0.027929577085632755 80-81 0.012899202268464928 82-83 0.03735160309042453 84-85 0.024115899893217038 86-87 0.03275275706427617 88-89 0.024676734774454645 90-91 0.02927558080060301 92-93 0.026471406394414983 94-95 0.0164885455083856 96-97 0.01716154736587073 98-99 0.012674868315969886 100-101 0.011328864600999631 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 445764.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.134695020647488 #Duplication Level Percentage of deduplicated Percentage of total 1 69.79787503394047 16.147525519994783 2 12.254794680039332 5.670218749267266 3 4.857031381194811 3.3709781912896855 4 2.529893530083964 2.3411326101280707 5 1.677243107402382 1.9401253882618603 6 1.1187565642763986 1.5529255150129135 7 0.8445436102764772 1.3676781198768009 8 0.7059457383607686 1.306547148648175 9 0.5095172275487271 1.0608773100395086 >10 4.513863808945112 21.99399963892532 >50 0.7368556113495808 11.857189279849885 >100 0.4237667588952258 17.15004185418854 >500 0.01495647384336091 2.4726121919518875 >1k 0.010968080818464667 4.623201189579226 >5k 0.003988393024896243 7.144947292986077 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9071 2.034933283082528 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8691 1.949686381134412 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7629 1.7114437235846773 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5583 1.2524564567798209 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2850 0.6393517646108704 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2627 0.5893252932044759 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2443 0.5480478459453881 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 2402 0.5388501538930914 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1812 0.4064931219210165 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1569 0.35197997146472126 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1464 0.3284249064527418 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 1405 0.31518920325553434 TruSeq Adapter, Index 11 (95% over 21bp) GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1229 0.2757064276164069 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1220 0.2736874220439515 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1021 0.2290449654974381 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 973 0.21827693577767607 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 844 0.18933785590581564 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 784 0.1758778187561131 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 777 0.1743074810886478 RNA PCR Primer, Index 11 (95% over 23bp) TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 736 0.16510978903635107 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 728 0.16331511741639074 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 720 0.16152044579643038 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 715 0.1603987760339552 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 695 0.15591209698405434 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 673 0.15097675002916341 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 657 0.14738740678924273 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 655 0.14693873888425266 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 639 0.143349395644332 No Hit TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG 609 0.1366193770694807 TruSeq Adapter, Index 11 (96% over 28bp) GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 514 0.11530765158245171 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 477 0.10700729534013514 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 466 0.10453962186268968 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 458 0.10274495024272932 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 452 0.10139894652775909 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 452 0.10139894652775909 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 450 0.100950278622769 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.121669762475211E-4 0.0 14-15 0.0 0.0 0.0 4.486679049900844E-4 0.0 16-17 0.0 0.0 0.0 8.973358099801688E-4 0.0 18-19 0.0 0.0 0.0 0.0016825046437128166 0.0 20-21 0.0 0.0 0.0 0.0028041744061880276 0.0 22-23 0.0 0.0 0.0 0.006617851598603746 0.0 24-25 0.0 0.0 0.0 0.015703376674652957 0.0 26-27 0.0 0.0 0.0 0.036566434256691885 0.0 28-29 0.0 0.0 0.0 0.06954352527346308 0.0 30-31 0.0 0.0 0.0 0.1503037481716783 0.0 32-33 0.0 0.0 0.0 0.26976157787528826 0.0 34-35 0.0 0.0 0.0 0.435880869697867 0.0 36-37 0.0 0.0 0.0 0.6640284993853249 0.0 38-39 0.0 0.0 0.0 0.9708051794222952 0.0 40-41 0.0 0.0 0.0 1.2689450022882063 0.0 42-43 0.0 0.0 0.0 1.5448757638571082 0.0 44-45 0.0 0.0 0.0 1.8740858391435826 0.0 46-47 0.0 0.0 0.0 2.239638014734254 0.0 48-49 0.0 0.0 0.0 2.588140809935302 0.0 50-51 0.0 0.0 0.0 2.93484893351639 0.0 52-53 0.0 0.0 0.0 3.2623765041591515 0.0 54-55 0.0 0.0 0.0 3.6164876481725754 0.0 56-57 0.0 0.0 0.0 4.031168959359661 0.0 58-59 0.0 0.0 0.0 4.436989079423192 0.0 60-61 0.0 0.0 0.0 4.852006891539021 0.0 62-63 0.0 0.0 0.0 5.23214077404187 0.0 64-65 0.0 0.0 0.0 5.638746062939134 0.0 66-67 0.0 0.0 0.0 6.090554643264149 0.0 68-69 0.0 0.0 0.0 6.570629301603539 0.0 70-71 0.0 0.0 0.0 7.083120216078463 0.0 72-73 0.0 0.0 0.0 7.552202510745596 0.0 74-75 0.0 0.0 0.0 8.06469342522052 0.0 76-77 0.0 0.0 0.0 8.588513204296444 0.0 78-79 0.0 0.0 0.0 9.190289031864395 0.0 80-81 0.0 0.0 0.0 9.802945056128355 0.0 82-83 0.0 0.0 0.0 10.378361644278138 0.0 84-85 0.0 0.0 0.0 10.952656562665446 0.0 86-87 0.0 0.0 0.0 11.533569332651357 0.0 88-89 0.0 0.0 0.0 12.152394540608933 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGCAC 25 0.005021119 56.994682 9 GTAAGCG 25 0.0016163622 37.996456 94-95 GGTCCTA 65 1.3276088E-4 36.535053 8 GAGATGT 55 0.0025734478 34.557735 1 GTAATAC 70 2.0510945E-4 33.929214 3 GTGGTAC 105 1.8929586E-6 31.677927 1 TATAGGG 60 0.003943679 31.674372 2 CTATACA 135 1.1947122E-8 31.663713 4 ATGGGGA 960 0.0 31.168966 5 TGGGGAA 815 0.0 29.721153 6 CATGGGG 1540 0.0 29.299215 4 ATGGGCG 65 0.005842948 29.228043 5 GGTACTC 100 4.9457627E-5 28.500538 3 GTACATG 3495 0.0 28.279007 1 GTATAGG 220 1.8189894E-12 28.078161 1 GGCCTCC 85 6.404473E-4 27.93857 8 GGGTCCT 70 0.008389971 27.140327 7 TACATGG 3595 0.0 26.43202 2 GGGAACT 90 8.943791E-4 26.386427 7 ACATGGG 3385 0.0 26.38159 3 >>END_MODULE