FastQCFastQC Report
Thu 26 May 2016
SRR938247_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938247_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891077
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65980.740452284146039No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52580.5900724628735788No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34320.38515190045304726No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13040.14633976637260304No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12060.13534183914521417No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10940.12277277945676973No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9800.10997927227388879No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30800.045.201931
GTATCAA51850.039.540721
TATCAAC64950.031.2415282
ATCAACG65550.030.9555633
TCAACGC66700.030.5643374
CAACGCA67650.029.9243935
AACGCAG69450.029.3507926
TAACGCA700.00837269327.154734
GTACATG55900.026.440811
ATAACGC908.923144E-426.4004333
TACATGG54750.026.2123762
ACGCAGA78350.025.707757
CGCAGAG78750.025.5771738
GCAGAGT82250.024.0831329
ACATGGG56950.023.9481373
GTAGGAC2402.2737368E-1023.7603913
GTGTAAG3650.023.512811
GAGTACT43050.023.50146512-13
TACCTGG7950.022.1165892
GTACTTT46050.022.0735714-15
Produced by FastQC (version 0.11.5)