FastQCFastQC Report
Thu 26 May 2016
SRR938247_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938247_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891077
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61680.6921960728421899No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54570.6124049885700114No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34850.3910997590556148No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14030.15744991734721017No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12190.13680074785905144No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10830.12153831823736892No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9270.10403141367132133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33250.046.642791
GTATCAA53750.037.7927061
TATTCCG600.00392421531.7098435
TATCAAC67200.030.0869562
ATCAACG67250.029.919743
TCAACGC68450.029.4630554
TAGACCG650.005804594629.2706245
CAACGCA69450.029.038825
AACGCAG70800.028.4435546
ACACCGT1703.6434358E-927.9384546
GTACGAC856.4096914E-427.9384546
TACCTGG7650.027.3621252
GGACCGA2700.026.3863186
TACATGG52050.025.5915072
ACGCAGA79400.025.2431327
GTACATG53600.025.1177221
CGCAGAG79950.025.010078
ACATGGG53400.024.140033
GTACCTG8900.024.0536561
GAGTACT47250.023.82307612-13
Produced by FastQC (version 0.11.5)