##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938247_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 891077 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.35178665816759 33.0 31.0 34.0 31.0 34.0 2 32.58003853763479 34.0 31.0 34.0 31.0 34.0 3 32.63995591851209 34.0 31.0 34.0 31.0 34.0 4 36.14303702149197 37.0 35.0 37.0 35.0 37.0 5 36.06969206926001 37.0 35.0 37.0 35.0 37.0 6 36.134155634137116 37.0 36.0 37.0 35.0 37.0 7 36.10273298491601 37.0 36.0 37.0 35.0 37.0 8 36.10264769486812 37.0 36.0 37.0 35.0 37.0 9 37.75707262110906 39.0 38.0 39.0 35.0 39.0 10-11 37.72698431224238 39.0 37.5 39.0 35.0 39.0 12-13 37.62409365296153 39.0 37.0 39.0 35.0 39.0 14-15 38.78422403451105 40.0 38.0 41.0 35.0 41.0 16-17 38.684071634662324 40.0 38.0 41.0 34.5 41.0 18-19 38.971981097032014 40.0 38.5 41.0 35.0 41.0 20-21 38.95564300279325 40.0 38.0 41.0 35.0 41.0 22-23 38.828730850420335 40.0 38.0 41.0 35.0 41.0 24-25 38.69673103446728 40.0 38.0 41.0 34.0 41.0 26-27 38.50771818821494 40.0 38.0 41.0 34.0 41.0 28-29 38.35875014168248 40.0 38.0 41.0 34.0 41.0 30-31 38.08144694566238 40.0 37.5 41.0 33.5 41.0 32-33 37.8115802562517 40.0 37.0 41.0 33.0 41.0 34-35 37.68368390161569 40.0 37.0 41.0 33.0 41.0 36-37 37.60339847173701 40.0 37.0 41.0 33.0 41.0 38-39 37.38977215212602 40.0 36.0 41.0 32.5 41.0 40-41 37.130418022236015 39.5 36.0 41.0 31.5 41.0 42-43 36.82690833676551 39.0 35.5 41.0 31.0 41.0 44-45 36.479981527971205 39.0 35.0 41.0 30.5 41.0 46-47 36.001911731533866 39.0 35.0 40.0 29.5 41.0 48-49 35.49210225378951 39.0 35.0 40.0 27.5 41.0 50-51 35.06005709944259 38.0 34.5 40.0 26.5 41.0 52-53 34.47590163364109 38.0 34.0 40.0 24.0 41.0 54-55 34.04500901717809 38.0 33.0 40.0 23.0 41.0 56-57 33.62992928781688 37.0 33.0 40.0 22.5 41.0 58-59 33.077569615196 36.5 32.0 39.5 20.0 41.0 60-61 32.55482915617843 36.0 31.5 39.0 18.0 40.0 62-63 31.95483274733833 35.5 31.0 39.0 13.5 40.0 64-65 31.324117332172193 35.0 30.0 38.0 8.0 40.0 66-67 30.718635426568074 35.0 29.0 38.0 6.5 40.0 68-69 30.047543029390276 34.0 28.5 37.5 2.0 39.5 70-71 29.392533978545064 34.0 28.0 37.0 2.0 39.0 72-73 28.668919184312916 34.0 26.0 36.0 2.0 39.0 74-75 27.980630742348865 33.0 26.0 35.5 2.0 38.0 76-77 26.732377785533686 31.5 25.0 34.5 2.0 36.0 78-79 27.37484863822094 33.0 26.0 35.0 2.0 37.0 80-81 27.050413151725387 33.0 25.5 35.0 2.0 36.5 82-83 26.439441821526085 32.5 24.5 35.0 2.0 36.0 84-85 25.725986643129605 32.0 21.0 35.0 2.0 35.5 86-87 24.932907032725566 31.5 18.5 34.0 2.0 35.0 88-89 24.049413238137667 31.0 10.0 34.0 2.0 35.0 90-91 23.323363188590886 30.5 4.5 34.0 2.0 35.0 92-93 22.307866211337515 29.5 2.0 34.0 2.0 35.0 94-95 21.319965053525117 29.0 2.0 34.0 2.0 35.0 96-97 20.1944770205044 28.0 2.0 34.0 2.0 35.0 98-99 18.697552512296916 26.0 2.0 33.0 2.0 35.0 100-101 15.137365233307559 10.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14023502896291262 1101 2 0.16433215100997245 1101 3 0.1545990130147672 1101 4 0.005484480518269663 1101 5 -0.04394138358892263 1101 6 -0.039392829622343584 1101 7 -0.007992283878117235 1101 8 0.04985744504294587 1101 9 0.019791752485311065 1101 10-11 0.06925795774785115 1101 12-13 0.24680183155570745 1101 14-15 0.4152947776444549 1101 16-17 0.35085970217662066 1101 18-19 0.30344675624619555 1101 20-21 0.15963045075452698 1101 22-23 0.23632194014719943 1101 24-25 0.332706613170231 1101 26-27 0.2303099516767304 1101 28-29 0.2525482681664144 1101 30-31 0.3578734051655488 1101 32-33 0.39293655279542605 1101 34-35 0.38302751255529444 1101 36-37 0.30183399865879323 1101 38-39 0.3053453570261482 1101 40-41 0.5878210885551383 1101 42-43 0.8039126406916779 1101 44-45 0.66795203462366 1101 46-47 0.751153914088242 1101 48-49 1.0358569022152864 1101 50-51 0.9128870348456388 1101 52-53 1.031322835440342 1101 54-55 1.0051884340849497 1101 56-57 1.1957136362868042 1101 58-59 1.4266911166844949 1101 60-61 1.2900051311896945 1101 62-63 1.5808585395144945 1101 64-65 1.9301693087480238 1101 66-67 1.9928242464960597 1101 68-69 2.4352427206307787 1101 70-71 2.1576113412661826 1101 72-73 2.529438212577684 1101 74-75 2.3005531279245837 1101 76-77 2.395606614740913 1101 78-79 2.2834406086331818 1101 80-81 2.7000437777563526 1101 82-83 2.9299640553826336 1101 84-85 3.058472713267509 1101 86-87 3.41096783774746 1101 88-89 3.916357317755807 1101 90-91 4.1517110308718514 1101 92-93 4.4259817039052365 1101 94-95 4.8063312167733905 1101 96-97 4.846070232831973 1101 98-99 5.0565922326185735 1101 100-101 4.7267710670749015 1102 1 0.24000570815492495 1102 2 0.15179707322060665 1102 3 0.24052289325185683 1102 4 0.09045759330631853 1102 5 0.04293940799025364 1102 6 0.028070187981441563 1102 7 0.1771892604815548 1102 8 0.16619820378301853 1102 9 0.3143852802011793 1102 10-11 0.3084166744729089 1102 12-13 0.4018798359578071 1102 14-15 0.4865462732984085 1102 16-17 0.5853290925334704 1102 18-19 0.552665329849269 1102 20-21 0.4899000294216975 1102 22-23 0.4004391471948594 1102 24-25 0.3793564250903074 1102 26-27 0.2937725269202218 1102 28-29 0.5130823567890062 1102 30-31 0.41460534557592865 1102 32-33 0.27385331379645095 1102 34-35 0.3116354753478845 1102 36-37 0.33553862792859945 1102 38-39 0.5379423959768914 1102 40-41 0.6812474301893872 1102 42-43 0.6548671712752068 1102 44-45 0.5551532302252511 1102 46-47 0.3184641023524861 1102 48-49 0.45011426682308553 1102 50-51 0.5280842614923174 1102 52-53 0.5751218809865648 1102 54-55 0.8665975666747698 1102 56-57 1.021122627567081 1102 58-59 1.0189385988403217 1102 60-61 0.9104054672606452 1102 62-63 1.116324162128933 1102 64-65 1.126217863934773 1102 66-67 1.2583579280857258 1102 68-69 1.5143430396524273 1102 70-71 1.5165775083831434 1102 72-73 1.715105892474714 1102 74-75 1.8071908363753515 1102 76-77 2.0313213722184145 1102 78-79 2.130281802821674 1102 80-81 2.490173767173495 1102 82-83 2.048042767868406 1102 84-85 2.41578002926175 1102 86-87 2.5340691827992465 1102 88-89 2.7592894599930737 1102 90-91 3.1793247581720863 1102 92-93 3.5610436727464823 1102 94-95 3.996141416557432 1102 96-97 3.98064760210913 1102 98-99 4.236591403193014 1102 100-101 3.703035672260656 1103 1 0.1429786702582092 1103 2 0.24001352577501933 1103 3 0.1462134340097947 1103 4 0.11595284759813751 1103 5 0.011108403462870342 1103 6 0.060686877246723725 1103 7 0.14905255420986663 1103 8 0.011168385108135226 1103 9 0.24327434783613455 1103 10-11 0.308064049969893 1103 12-13 0.23208431507899263 1103 14-15 0.39903219704533655 1103 16-17 0.5496242457060845 1103 18-19 0.2704264012532178 1103 20-21 0.31404094707848884 1103 22-23 0.3531393459860226 1103 24-25 0.24914749327682273 1103 26-27 0.4985525639206614 1103 28-29 0.3361869462220497 1103 30-31 0.5183868304195371 1103 32-33 0.6295982923086214 1103 34-35 0.6888855213111569 1103 36-37 0.6464866492095993 1103 38-39 0.7654160820017566 1103 40-41 0.8545059965948312 1103 42-43 1.0196029973501766 1103 44-45 0.9544484121249113 1103 46-47 0.8269579720006348 1103 48-49 0.8917064346809624 1103 50-51 0.9065372525524609 1103 52-53 0.9468908063651824 1103 54-55 1.2634401769291799 1103 56-57 1.409339941932906 1103 58-59 1.4697097764194424 1103 60-61 1.5447927480732702 1103 62-63 1.5457949524674603 1103 64-65 1.667979808640954 1103 66-67 1.6646854911128663 1103 68-69 1.6438122500033536 1103 70-71 1.9783288815359974 1103 72-73 2.232913788965419 1103 74-75 2.1681081197159635 1103 76-77 2.1697685029609524 1103 78-79 2.2801393166681088 1103 80-81 2.403666573920905 1103 82-83 2.691476195809482 1103 84-85 3.151052390395204 1103 86-87 3.474653031304289 1103 88-89 3.445926930404216 1103 90-91 3.7558593570275995 1103 92-93 3.8133310638709723 1103 94-95 3.553104278230954 1103 96-97 4.138516371212617 1103 98-99 4.100597562271908 1103 100-101 3.5008288605032494 1104 1 0.24310793631124739 1104 2 0.2417038228956372 1104 3 0.19615030407691592 1104 4 0.1542762696231108 1104 5 -0.01691609423055951 1104 6 0.1477078003101937 1104 7 0.18851422805494877 1104 8 0.10523335154815783 1104 9 0.17118597686169323 1104 10-11 0.25624899607714724 1104 12-13 0.2871235048295375 1104 14-15 0.5016142386291094 1104 16-17 0.36273504830342773 1104 18-19 0.32230089566717623 1104 20-21 0.4109378785811444 1104 22-23 0.23499003686251996 1104 24-25 0.4329369016591684 1104 26-27 0.5252194947674198 1104 28-29 0.5781522274918629 1104 30-31 0.41719241755524905 1104 32-33 0.5236800660684295 1104 34-35 0.40964146809617574 1104 36-37 0.6661778591179939 1104 38-39 0.8249344907995066 1104 40-41 0.8972157191510703 1104 42-43 1.1073126105905189 1104 44-45 1.0288795595046096 1104 46-47 1.0424681219820613 1104 48-49 1.1795570654337197 1104 50-51 1.3615455605609483 1104 52-53 1.5691531262672385 1104 54-55 1.6651446851169425 1104 56-57 1.4403856578427394 1104 58-59 1.7440004528662385 1104 60-61 1.8990685028995742 1104 62-63 2.088150764793916 1104 64-65 2.2011886129436284 1104 66-67 2.205174051203816 1104 68-69 2.316590814248311 1104 70-71 2.422707584612148 1104 72-73 2.427274728207639 1104 74-75 2.4081706210824123 1104 76-77 2.1479873096685758 1104 78-79 2.074160830037446 1104 80-81 2.2811466784269037 1104 82-83 2.8698326039076036 1104 84-85 2.8885691977149968 1104 86-87 2.854670631164364 1104 88-89 3.0865406572019154 1104 90-91 3.2735849303895 1104 92-93 3.409138935709013 1104 94-95 3.886094177441695 1104 96-97 4.153393582126718 1104 98-99 4.31840949519567 1104 100-101 3.7346309768590924 1105 1 0.23866257928329304 1105 2 0.25494442146904106 1105 3 0.30066262264644905 1105 4 0.18034549634808883 1105 5 0.06866781825907253 1105 6 0.11221164405962014 1105 7 0.16711923081449243 1105 8 0.16961582750347048 1105 9 0.2629834495866348 1105 10-11 0.3144806882423907 1105 12-13 0.2840749237079834 1105 14-15 0.43681063735370174 1105 16-17 0.4953470192483991 1105 18-19 0.43582686549786587 1105 20-21 0.27725674630332975 1105 22-23 0.3796563931345389 1105 24-25 0.3699668957215607 1105 26-27 0.421540925861116 1105 28-29 0.4450288900867818 1105 30-31 0.5927232054781015 1105 32-33 0.7306154246396304 1105 34-35 0.6576568169370418 1105 36-37 0.6480106174365261 1105 38-39 0.6912886702721295 1105 40-41 0.7226115377166806 1105 42-43 0.7775675614287181 1105 44-45 1.1044028927246003 1105 46-47 0.8995268101112899 1105 48-49 1.2264523778642342 1105 50-51 1.3407390924945375 1105 52-53 1.3874453977021233 1105 54-55 1.5368786751616383 1105 56-57 1.3514477829823548 1105 58-59 1.7039150769007279 1105 60-61 1.5674126956860377 1105 62-63 1.6757435235309543 1105 64-65 1.7925885015154357 1105 66-67 2.096008428631496 1105 68-69 2.440847632685756 1105 70-71 2.5184684743636296 1105 72-73 2.373874860561312 1105 74-75 2.158635559129909 1105 76-77 2.097022644960184 1105 78-79 2.1458384520657 1105 80-81 2.4332062689675418 1105 82-83 2.703027741871921 1105 84-85 3.0694967981638506 1105 86-87 3.5191449176708325 1105 88-89 3.3901922845504764 1105 90-91 3.6260092782337843 1105 92-93 3.9754772902860687 1105 94-95 4.199272130354128 1105 96-97 4.398326999859496 1105 98-99 4.522751401873254 1105 100-101 4.31612657335303 1106 1 0.21717397702389007 1106 2 0.14877987869587628 1106 3 0.1981191159751674 1106 4 0.09786748858946481 1106 5 0.020199655999462607 1106 6 0.06065449522522215 1106 7 0.16754538698911148 1106 8 0.16888232233019096 1106 9 0.06321951427973005 1106 10-11 0.15103688145384808 1106 12-13 0.13650608011504772 1106 14-15 0.09794416112288218 1106 16-17 0.07300946223386973 1106 18-19 -0.10081998275438053 1106 20-21 -0.013291368020169614 1106 22-23 -0.15559705356494646 1106 24-25 -0.03739966574049447 1106 26-27 -0.07277828319301705 1106 28-29 0.059277486354169184 1106 30-31 0.16120908550352198 1106 32-33 0.25949202530805593 1106 34-35 0.12151877332964034 1106 36-37 0.13167592212527524 1106 38-39 0.21385041126215043 1106 40-41 0.24896219451374435 1106 42-43 0.4312756367008106 1106 44-45 0.32260905933357975 1106 46-47 0.5979050921595288 1106 48-49 0.5632771786039399 1106 50-51 0.6756048662133551 1106 52-53 0.5803249737154701 1106 54-55 0.8087831025368786 1106 56-57 0.9479824875915526 1106 58-59 1.1437779837507378 1106 60-61 1.0647576135799497 1106 62-63 1.060774335913763 1106 64-65 1.1139477072636375 1106 66-67 1.155530823784165 1106 68-69 1.4338431748338891 1106 70-71 1.3959085413088914 1106 72-73 1.8639260509575628 1106 74-75 1.987293657905159 1106 76-77 1.9101402485677887 1106 78-79 2.19400313947369 1106 80-81 2.1890898069419293 1106 82-83 2.4219080100662076 1106 84-85 2.3004347033350605 1106 86-87 2.72883570908159 1106 88-89 2.504553231082081 1106 90-91 2.5927187327022274 1106 92-93 3.1459578394851526 1106 94-95 3.9365236038213887 1106 96-97 4.147579289480394 1106 98-99 4.170564316729834 1106 100-101 3.75842244454539 1107 1 0.287374841503933 1107 2 0.21328534526084297 1107 3 0.19468662169599327 1107 4 0.14071010237029213 1107 5 0.033853355465517154 1107 6 0.12343100170882337 1107 7 0.21473582685689507 1107 8 0.10374120867648884 1107 9 0.15543919391359395 1107 10-11 0.19656192594914756 1107 12-13 0.1980875700718272 1107 14-15 0.3371762994396903 1107 16-17 0.3838154627423833 1107 18-19 0.36838927226722973 1107 20-21 0.18299645298161948 1107 22-23 0.18310114119731935 1107 24-25 0.276965958464956 1107 26-27 0.43688355255576994 1107 28-29 0.4093029122377132 1107 30-31 0.5694343588317636 1107 32-33 0.6509743543189899 1107 34-35 0.7195355544127793 1107 36-37 0.7478086452373987 1107 38-39 0.8575447921418871 1107 40-41 0.8842533208803829 1107 42-43 0.8494551434386679 1107 44-45 0.9577730562824698 1107 46-47 1.2066770219840848 1107 48-49 1.058560677205513 1107 50-51 0.9431614388042604 1107 52-53 1.1056110149053993 1107 54-55 1.149693349167947 1107 56-57 1.1469654522495816 1107 58-59 1.2493971040151592 1107 60-61 1.333890590950908 1107 62-63 1.629449647380838 1107 64-65 1.5885345366559598 1107 66-67 1.9081624027315378 1107 68-69 1.7258594473993618 1107 70-71 1.992870148224732 1107 72-73 2.1467254407363576 1107 74-75 1.9949087100282235 1107 76-77 2.043524433668221 1107 78-79 2.223662432634125 1107 80-81 2.2412382941018585 1107 82-83 2.513148793183422 1107 84-85 2.4682328218196794 1107 86-87 2.50149017132415 1107 88-89 2.750523228539489 1107 90-91 2.9078344204723763 1107 92-93 3.326646880929342 1107 94-95 3.276035426857238 1107 96-97 3.434217987675158 1107 98-99 3.6449096002284342 1107 100-101 3.807799510398427 1108 1 0.31573951818364066 1108 2 0.2504514018892223 1108 3 0.2854350755644717 1108 4 0.14912442354493294 1108 5 0.04960496470444298 1108 6 0.17904125035201446 1108 7 0.273371023962369 1108 8 0.1918643943384879 1108 9 0.22192915983559658 1108 10-11 0.2992734409476654 1108 12-13 0.3677371135948988 1108 14-15 0.5642283420161576 1108 16-17 0.4573376975860839 1108 18-19 0.44186943550506186 1108 20-21 0.4268539985241304 1108 22-23 0.5087332670755629 1108 24-25 0.48467558024121615 1108 26-27 0.5992168438175014 1108 28-29 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2.61760359100807 2316 84-85 2.8550641186089933 2316 86-87 2.742126050906375 2316 88-89 3.3016621003623676 2316 90-91 3.5312154248640297 2316 92-93 3.574212698846381 2316 94-95 4.111901356272217 2316 96-97 4.308816367541812 2316 98-99 4.627990925461102 2316 100-101 4.411096094920692 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 9.0 5 19.0 6 50.0 7 108.0 8 280.0 9 544.0 10 913.0 11 1492.0 12 2414.0 13 3655.0 14 4615.0 15 6696.0 16 8933.0 17 12527.0 18 12781.0 19 9868.0 20 9366.0 21 10238.0 22 11358.0 23 12646.0 24 13902.0 25 15373.0 26 17362.0 27 20251.0 28 24308.0 29 28972.0 30 33836.0 31 39709.0 32 47542.0 33 57903.0 34 73915.0 35 97489.0 36 120343.0 37 132564.0 38 57390.0 39 1704.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.4815655661632 18.977933444584476 14.567315731412661 21.97318525783967 2 16.06692691555679 20.48719220779804 39.813927281461645 23.631953595183525 3 19.87120783616595 25.08428277713434 29.571583124590372 25.472926262109336 4 13.020711171463361 16.946350715155457 35.972459921554545 34.06047819182663 5 13.451532260329765 37.81388163253841 33.40546613859161 15.329119968540208 6 31.89331561694444 35.667063564652665 17.256870057245333 15.182750761157566 7 28.791563467579124 33.44166665731469 19.6537448503328 18.113025024773393 8 26.962579903756374 34.55904797816562 19.08914206708324 19.389230050994758 9 27.074203463898183 16.614837999409705 19.945302145605822 36.36565639108629 10-11 25.51625729314077 26.895374922705894 26.075748784897378 21.512618999255956 12-13 26.426111323712764 24.345819721527995 27.172511466461373 22.055557488297868 14-15 23.13082932226957 25.30168548845947 26.25592401105628 25.311561178214674 16-17 21.882901253202586 28.882520814699514 26.794373550209468 22.440204381888435 18-19 21.82976330889474 26.61554500901718 30.12012429902242 21.434567383065662 20-21 22.813516677009954 25.574220858579 30.28571043804295 21.32655202636809 22-23 22.46359164737345 25.714672086349825 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22.28112722020656 48-49 22.14988154783481 26.58378568855441 28.934592633408784 22.33174013020199 50-51 22.240951118702423 26.912937939145547 28.704926734726627 22.141184207425397 52-53 22.536492357001695 26.795608011428868 28.514931930686128 22.152967700883313 54-55 22.04063172991784 26.84998041695611 28.741062781330907 22.36832507179514 56-57 22.165087865582887 27.22997002503712 28.424255142933774 22.180686966446224 58-59 22.267604258666758 26.704257881193204 28.42919298781138 22.598944872328655 60-61 22.106282622040517 26.834886322955253 28.735732153338038 22.323098901666185 62-63 22.089280724336955 26.865579517819448 28.631364068425064 22.413775689418536 64-65 21.94417541918375 26.82063390705854 28.53524442893263 22.699946244825085 66-67 21.913370000572343 27.035317935487058 28.371846652982853 22.67946541095775 68-69 22.071816027611792 26.772633080397295 28.505272041375047 22.650278850615862 70-71 21.968920755445374 26.962484723542413 28.334588368906388 22.734006152105824 72-73 22.056523766477962 26.90661239523206 28.143599343041814 22.893264495248165 74-75 21.975036949668773 27.19355341906479 27.9700856379415 22.86132399332493 76-77 22.021048686028255 26.93094985057408 28.299630671647908 22.748370791749757 78-79 22.04859961597034 27.072071212701033 28.25311392842594 22.62621524290269 80-81 21.871691151096453 27.33715904302268 28.1468538335373 22.644295972343564 82-83 22.130639765403266 27.275349274628784 27.9217696656009 22.67224129436705 84-85 22.042899429036375 27.045494718235762 27.983558492207116 22.928047360520743 86-87 22.076114781566446 27.016752165144673 28.00687728733212 22.900255765956754 88-89 22.05858752947276 27.153994548170363 27.7038909095398 23.083527012817072 90-91 21.891372769291284 27.19114893387793 27.99278160251342 22.924696694317365 92-93 22.070427134804287 26.869956243960956 27.90146081651754 23.15815580471721 94-95 21.80019235150273 26.923767530752112 27.90219027087446 23.373849846870698 96-97 21.95834025380551 26.84097652725469 27.766430697269367 23.43425252167043 98-99 21.81433254365223 26.82321505324456 27.519395069112996 23.843057333990217 100-101 22.261151393201708 26.355129803597222 27.634985528747798 23.748733274453272 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1216.0 1 816.0 2 692.5 3 887.0 4 630.5 5 510.5 6 901.5 7 1899.5 8 2855.5 9 3084.0 10 2439.5 11 1711.0 12 1566.0 13 1597.5 14 1694.0 15 1760.0 16 1785.0 17 1843.0 18 1911.5 19 1968.5 20 2097.0 21 2375.5 22 2685.5 23 3198.5 24 3832.0 25 4514.0 26 5504.5 27 6686.5 28 7523.0 29 8389.0 30 9664.5 31 11053.0 32 12612.0 33 13911.5 34 15106.0 35 16277.5 36 17738.5 37 19465.0 38 20817.0 39 21601.5 40 22599.5 41 24698.0 42 26499.5 43 27886.5 44 29454.0 45 30875.5 46 31914.5 47 33092.0 48 34796.5 49 35002.0 50 34167.5 51 33121.5 52 31681.5 53 30959.5 54 30639.0 55 28776.0 56 25918.0 57 23556.5 58 21495.0 59 19344.0 60 16567.5 61 13549.0 62 10810.0 63 8310.0 64 6371.0 65 5134.5 66 4059.5 67 3232.0 68 2480.5 69 1902.0 70 1519.5 71 1088.5 72 824.0 73 707.5 74 556.0 75 445.5 76 330.0 77 197.5 78 126.5 79 72.0 80 42.0 81 23.5 82 14.5 83 12.5 84 5.0 85 2.0 86 2.5 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015150205874464272 3 0.0032544886693293626 4 0.0013466849666190464 5 0.11805938207360307 6 0.0 7 0.0 8 5.611187360912693E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.488949888730154E-4 24-25 5.611187360912693E-4 26-27 0.005611187360912693 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010661255985734117 36-37 6.172306097003963E-4 38-39 0.00712620794835912 40-41 0.010044025376033721 42-43 0.005947858602567455 44-45 0.003983943026248012 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 9.539018513551579E-4 70-71 0.0 72-73 5.6111873609126925E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.6111873609126925E-5 82-83 0.0017955799554920616 84-85 0.004432838015121027 86-87 0.003142264922111108 88-89 0.0 90-91 0.0023005868179742043 92-93 0.0 94-95 0.0 96-97 1.1222374721825385E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 891077.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.90628481824112 #Duplication Level Percentage of deduplicated Percentage of total 1 83.5770884534493 53.411012189854624 2 10.152590214329912 12.976286437597102 3 2.664946461173895 5.10920482719428 4 1.1115957182091427 2.841518102824431 5 0.5909468292737985 1.888260819200394 6 0.36201365451557377 1.388096862813876 7 0.2576416570469986 1.1525444781402363 8 0.188226558749617 0.9623088059048336 9 0.13589972186232832 0.7816361698848332 >10 0.8562478505814313 10.29601276343614 >50 0.06912302517084905 3.0200091432671923 >100 0.03175028062284917 3.707654703500078 >500 8.770795519833038E-4 0.3562594927978009 >1k 7.01663641586643E-4 0.8060118795519787 >5k 3.508318207933215E-4 1.3031833240322326 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6168 0.6921960728421899 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5457 0.6124049885700114 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3485 0.3910997590556148 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1403 0.15744991734721017 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1219 0.13680074785905144 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1083 0.12153831823736892 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 927 0.10403141367132133 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 5.611187360912693E-5 0.0 0.0 0.0 0.0 20-21 1.1222374721825386E-4 0.0 0.0 0.0 0.0 22-23 1.1222374721825386E-4 0.0 0.0 1.1222374721825386E-4 0.0 24-25 1.1222374721825386E-4 0.0 0.0 8.41678104136904E-4 0.0 26-27 1.1222374721825386E-4 0.0 0.0 0.0012905730930099194 0.0 28-29 1.1222374721825386E-4 0.0 0.0 0.0036472717845932503 0.0 30-31 1.1222374721825386E-4 0.0 0.0 0.01408408027589086 0.0 32-33 1.1222374721825386E-4 0.0 0.0 0.035518815994577346 0.0 34-35 1.1222374721825386E-4 0.0 0.0 0.06346252905192257 0.0 36-37 1.1222374721825386E-4 0.0 0.0 0.11149429286133522 0.0 38-39 1.1222374721825386E-4 0.0 0.0 0.19033147528215855 0.0 40-41 1.1222374721825386E-4 0.0 0.0 0.2688319864613271 0.0 42-43 1.1222374721825386E-4 0.0 0.0 0.34677137890440446 0.0 44-45 1.1222374721825386E-4 0.0 0.0 0.43969264160111865 5.611187360912693E-5 46-47 1.1222374721825386E-4 0.0 0.0 0.5495596901277893 1.1222374721825386E-4 48-49 1.1222374721825386E-4 0.0 0.0 0.6649818141417633 2.8055936804563465E-4 50-51 1.1222374721825386E-4 0.0 0.0 0.784219545561158 3.366712416547616E-4 52-53 1.1222374721825386E-4 0.0 0.0 0.8950404959391838 3.366712416547616E-4 54-55 1.1222374721825386E-4 0.0 0.0 1.0174766041542986 3.366712416547616E-4 56-57 1.1222374721825386E-4 5.611187360912693E-5 0.0 1.1577001763035069 3.366712416547616E-4 58-59 1.1222374721825386E-4 2.2444749443650773E-4 0.0 1.3101000250258956 3.366712416547616E-4 60-61 1.1222374721825386E-4 3.366712416547616E-4 0.0 1.47821119835884 3.366712416547616E-4 62-63 1.1222374721825386E-4 3.9278311526388856E-4 0.0 1.6437973373793735 3.366712416547616E-4 64-65 1.1222374721825386E-4 5.050068624821424E-4 0.0 1.809327364526298 3.366712416547616E-4 66-67 1.1222374721825386E-4 5.611187360912693E-4 0.0 1.9939354287003255 3.366712416547616E-4 68-69 1.1222374721825386E-4 5.611187360912693E-4 0.0 2.199641557351385 3.366712416547616E-4 70-71 1.1222374721825386E-4 5.611187360912693E-4 0.0 2.4233034855573647 3.366712416547616E-4 72-73 1.1222374721825386E-4 5.611187360912693E-4 0.0 2.641578673896869 3.366712416547616E-4 74-75 1.1222374721825386E-4 5.611187360912693E-4 0.0 2.8608638759613365 3.366712416547616E-4 76-77 1.1222374721825386E-4 5.611187360912693E-4 0.0 3.088285299699128 3.366712416547616E-4 78-79 1.1222374721825386E-4 5.611187360912693E-4 0.0 3.345053233334493 3.366712416547616E-4 80-81 1.1222374721825386E-4 5.611187360912693E-4 0.0 3.6285304188078022 3.366712416547616E-4 82-83 1.1222374721825386E-4 5.611187360912693E-4 0.0 3.9141398554782585 3.366712416547616E-4 84-85 1.1222374721825386E-4 5.611187360912693E-4 0.0 4.176294528980099 3.366712416547616E-4 86-87 1.1222374721825386E-4 5.611187360912693E-4 0.0 4.457471127635435 3.366712416547616E-4 88-89 1.1222374721825386E-4 5.611187360912693E-4 0.0 4.749028422908458 3.366712416547616E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3325 0.0 46.64279 1 GTATCAA 5375 0.0 37.792706 1 TATTCCG 60 0.003924215 31.709843 5 TATCAAC 6720 0.0 30.086956 2 ATCAACG 6725 0.0 29.91974 3 TCAACGC 6845 0.0 29.463055 4 TAGACCG 65 0.0058045946 29.270624 5 CAACGCA 6945 0.0 29.03882 5 AACGCAG 7080 0.0 28.443554 6 ACACCGT 170 3.6434358E-9 27.938454 6 GTACGAC 85 6.4096914E-4 27.938454 6 TACCTGG 765 0.0 27.362125 2 GGACCGA 270 0.0 26.386318 6 TACATGG 5205 0.0 25.591507 2 ACGCAGA 7940 0.0 25.243132 7 GTACATG 5360 0.0 25.117722 1 CGCAGAG 7995 0.0 25.01007 8 ACATGGG 5340 0.0 24.14003 3 GTACCTG 890 0.0 24.053656 1 GAGTACT 4725 0.0 23.823076 12-13 >>END_MODULE