FastQCFastQC Report
Thu 26 May 2016
SRR938246_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938246_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883786
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65280.738640349586891No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52900.5985611901523672No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34220.38719780580366736No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13480.1525256114036656No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11730.13272443781639445No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10850.12276727624108098No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10220.11563885374966337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30700.048.9607161
GTATCAA52650.040.2402041
ATCAACG65250.032.2680973
TATCAAC65500.032.144942
TCAACGC66550.031.6377664
CAACGCA66950.031.3777525
AACGCAG69550.030.4063176
TAGCGCG1955.311449E-1026.8107054
ACGCAGA78550.026.669897
CGCAGAG78600.026.5924858
GCAGAGT84950.024.3237259
GAGTACT44550.023.9378512-13
GTACATG55800.023.773121
CGTACTG1000.001647667723.7640345
TACATGG55450.023.7426052
ACATGGG56300.023.1308883
GTACTTT47600.022.35918214-15
TGTAGCG2354.612957E-922.2471792
TACCTGG7800.021.9360312
GTGGTAT16050.021.7031821
Produced by FastQC (version 0.11.5)