FastQCFastQC Report
Thu 26 May 2016
SRR938246_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938246_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883786
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64720.7323039740389642No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53460.604897565700294No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35260.3989653603926742No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14070.15920143564165987No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12420.14053175768794707No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11250.1272932587753144No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10460.11835444327020343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32300.049.2615661
GTATCAA52300.037.961251
ATAACGC902.413388E-531.6646483
ATCAACG65200.030.3776653
TCAACGC65900.030.1991394
TATCAAC65600.030.1924342
TATAACG953.4914596E-529.9980892
CAACGCA67400.029.5975195
AACGCAG69600.028.730216
TACCTGG7700.028.3748172
GTACCTG8650.025.8076041
ACGCAGA78750.025.3317187
GTACATG55400.025.291711
TACATGG54050.025.2204112
CGCAGAG79400.025.0047058
GAGTACT48000.024.29272312-13
GACGGAC1000.001653957923.7484867
GCAGAGT85100.023.1624499
ACATGGG56050.023.1341173
TGGTATC15650.023.0656242
Produced by FastQC (version 0.11.5)