FastQCFastQC Report
Thu 26 May 2016
SRR938245_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938245_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891792
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68140.764079516299765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53370.5984579363797836No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36340.40749412419039416No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13600.152501928700863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12360.13859734108401958No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10940.12267434558731183No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10030.11247017241688645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29900.049.7786031
GTATCAA51600.039.2914961
TATAACG851.6271611E-533.543342
ATCAACG64250.031.5812053
TATCAAC64500.031.45882
TCAACGC65250.031.0972044
CAACGCA66400.030.5586225
ACCTCGT804.4672092E-429.7165138
AACGCAG68900.029.380856
GGACCGA2900.026.2177836
ACGCAGA77900.026.062037
CGCAGAG78250.025.7024088
TAACGCA1501.0558342E-625.3438574
GGACCGT950.001223372825.0103846
ACCGAGT3250.024.8704368
GTACATG53550.024.4981081
GAGTACT44250.024.43859312-13
TACATGG52850.024.186962
AGGACCG4000.023.7598655
GCAGAGT83800.023.4327819
Produced by FastQC (version 0.11.5)