##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938245_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 891792 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.007052092864704 33.0 31.0 34.0 30.0 34.0 2 32.175219109388735 34.0 31.0 34.0 30.0 34.0 3 32.234374158996715 34.0 31.0 34.0 30.0 34.0 4 35.77251197588675 37.0 35.0 37.0 35.0 37.0 5 35.800140615748965 37.0 35.0 37.0 35.0 37.0 6 35.84108962628057 37.0 36.0 37.0 35.0 37.0 7 35.78850449432154 37.0 36.0 37.0 35.0 37.0 8 35.78061700486212 37.0 36.0 37.0 35.0 37.0 9 37.45171295548738 39.0 38.0 39.0 35.0 39.0 10-11 37.37223422053573 39.0 37.0 39.0 35.0 39.0 12-13 37.27391869404525 39.0 37.0 39.0 34.0 39.0 14-15 38.51652627518524 40.0 38.0 41.0 34.0 41.0 16-17 38.43962269228699 40.0 38.0 41.0 34.0 41.0 18-19 38.40663405816603 40.0 38.0 41.0 34.0 41.0 20-21 38.32227414015824 40.0 38.0 41.0 34.0 41.0 22-23 38.159235561655635 40.0 38.0 41.0 34.0 41.0 24-25 38.10188306241814 40.0 38.0 41.0 33.5 41.0 26-27 38.11836728743923 40.0 38.0 41.0 34.0 41.0 28-29 38.020813149254536 40.0 38.0 41.0 33.0 41.0 30-31 37.808710439205555 40.0 37.5 41.0 33.0 41.0 32-33 37.56448869243052 40.0 37.0 41.0 33.0 41.0 34-35 37.339675619426956 40.0 36.5 41.0 32.0 41.0 36-37 37.118339814665305 40.0 36.0 41.0 31.5 41.0 38-39 36.87569522938084 39.5 36.0 41.0 31.0 41.0 40-41 36.63998219315715 39.0 35.0 41.0 30.5 41.0 42-43 36.32536791090299 39.0 35.0 41.0 30.0 41.0 44-45 36.04100675942372 39.0 35.0 40.5 29.5 41.0 46-47 35.80611678507993 39.0 35.0 41.0 28.5 41.0 48-49 35.50570424493604 39.0 35.0 41.0 27.0 41.0 50-51 34.85109812602042 38.5 34.5 40.0 25.5 40.5 52-53 34.9270900613596 38.5 35.0 40.0 26.0 41.0 54-55 35.06404015734611 39.0 35.0 40.0 26.0 41.0 56-57 34.861913428243355 38.5 35.0 40.0 25.5 41.0 58-59 34.604956649084095 38.0 34.5 40.0 25.0 41.0 60-61 34.24186918025728 38.0 34.0 40.0 23.5 41.0 62-63 33.8272130721065 37.0 34.0 40.0 22.5 41.0 64-65 33.39600153398999 36.5 33.0 39.5 21.5 41.0 66-67 32.96927030069792 36.0 33.0 39.0 19.5 41.0 68-69 32.494304725765645 35.5 32.5 39.0 18.0 40.5 70-71 32.01700564705671 35.0 32.0 38.5 14.0 40.0 72-73 31.529497909826507 35.0 31.0 37.5 9.5 39.5 74-75 31.006315373988556 35.0 31.0 37.0 7.0 39.0 76-77 30.46458703374778 35.0 30.5 36.0 3.5 39.0 78-79 29.89813039363439 34.0 30.0 36.0 2.0 38.0 80-81 29.370795544252474 34.0 29.0 35.0 2.0 37.0 82-83 28.837922407915748 34.0 29.0 35.0 2.0 37.0 84-85 28.342332629133253 34.0 28.0 35.0 2.0 36.0 86-87 27.83324755099844 33.5 27.0 35.0 2.0 36.0 88-89 27.285437635681863 33.0 26.0 35.0 2.0 35.0 90-91 26.6676708245869 33.0 25.0 35.0 2.0 35.0 92-93 26.03184935500655 32.0 23.5 35.0 2.0 35.0 94-95 25.42354607352387 32.0 21.5 34.5 2.0 35.0 96-97 24.568603441161166 32.0 12.5 34.0 2.0 35.0 98-99 23.483978887453578 31.0 2.0 34.0 2.0 35.0 100-101 20.595037856361124 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25656421695575915 1101 2 0.1780325600468089 1101 3 0.17696613358945257 1101 4 0.104743302668318 1101 5 0.12137491128646616 1101 6 0.07898006750679798 1101 7 0.05088294195054743 1101 8 0.07389455051737315 1101 9 0.07953946837570669 1101 10-11 0.15769832054856892 1101 12-13 0.020588755909081158 1101 14-15 0.182067891447538 1101 16-17 0.22833848003335078 1101 18-19 0.0035809754521807236 1101 20-21 0.1296396403852853 1101 22-23 0.26230876231724665 1101 24-25 0.18543350242037349 1101 26-27 0.3438696556936165 1101 28-29 0.3637099290838748 1101 30-31 0.2926594389587507 1101 32-33 0.36197777243007323 1101 34-35 0.3135781281292438 1101 36-37 0.2530426666129699 1101 38-39 0.23912992165141844 1101 40-41 0.3479552049351611 1101 42-43 0.31735690031431574 1101 44-45 0.4579337323854489 1101 46-47 0.4268327097365585 1101 48-49 0.29345431415195833 1101 50-51 0.5002759803482846 1101 52-53 0.5988822489988408 1101 54-55 0.5116073037183 1101 56-57 0.5584992985995925 1101 58-59 0.45139961596103717 1101 60-61 0.3982578787211679 1101 62-63 0.4794199336739169 1101 64-65 0.47407485416898254 1101 66-67 0.30910403819191146 1101 68-69 0.5013008852038325 1101 70-71 0.46489747265493264 1101 72-73 0.5783911127727954 1101 74-75 0.5991730286195178 1101 76-77 0.6476043442756279 1101 78-79 0.8891762859628507 1101 80-81 1.0908688394511863 1101 82-83 0.7225009539483231 1101 84-85 0.9594253654231686 1101 86-87 1.1388123402621062 1101 88-89 0.9372056821868391 1101 90-91 1.0747877255356144 1101 92-93 1.0109286035738947 1101 94-95 1.1119544461384763 1101 96-97 1.2985361258839951 1101 98-99 1.535784309861615 1101 100-101 1.5165934524185012 1102 1 0.31052899813450097 1102 2 0.21188610146337084 1102 3 0.1638645615997092 1102 4 0.014123386062621535 1102 5 -0.03606273832959772 1102 6 0.15699423175237825 1102 7 0.09573685984906177 1102 8 0.10079241025298558 1102 9 0.3216059991300213 1102 10-11 0.328542279433897 1102 12-13 0.19747702601001293 1102 14-15 0.24671055324598967 1102 16-17 0.27740544409707724 1102 18-19 0.11543376390484639 1102 20-21 0.3747769852640701 1102 22-23 0.4486495708299998 1102 24-25 0.28512237205106317 1102 26-27 0.24854868706947997 1102 28-29 0.2991596119155773 1102 30-31 0.3320046444965854 1102 32-33 0.4317931542040867 1102 34-35 0.4060790338169653 1102 36-37 0.6620526611433277 1102 38-39 0.7365229617076707 1102 40-41 0.7612372041253721 1102 42-43 0.564534351176313 1102 44-45 0.5826202934003888 1102 46-47 0.6656735715250619 1102 48-49 0.7047388279574847 1102 50-51 0.8514682915923046 1102 52-53 0.7222781162214673 1102 54-55 0.6584878310771671 1102 56-57 0.6009593595470477 1102 58-59 0.5516198223868045 1102 60-61 0.691771733967407 1102 62-63 0.817961030586055 1102 64-65 0.8154587450034185 1102 66-67 0.7854005923165133 1102 68-69 0.8934295709976752 1102 70-71 0.713975802211614 1102 72-73 0.8526049403156399 1102 74-75 0.7399012725798251 1102 76-77 0.5078336430957471 1102 78-79 0.6293952137706569 1102 80-81 0.9590831074068866 1102 82-83 1.0051145911191703 1102 84-85 1.007553255632505 1102 86-87 0.9933354512816592 1102 88-89 0.9935508237937754 1102 90-91 1.18084056087093 1102 92-93 1.222616592378884 1102 94-95 1.5138048904582178 1102 96-97 1.4438780496357424 1102 98-99 1.341884027855322 1102 100-101 0.9029825642762646 1103 1 0.46229356751950945 1103 2 0.42088264125575847 1103 3 0.2584340148596027 1103 4 0.18783536302186832 1103 5 0.2802384567046303 1103 6 0.342662010872381 1103 7 0.4181121865895747 1103 8 0.37366151612715015 1103 9 0.41138985558941954 1103 10-11 0.454828937501226 1103 12-13 0.3833338539482938 1103 14-15 0.47649154326121135 1103 16-17 0.4102583067875827 1103 18-19 0.40136361792093567 1103 20-21 0.5187011473410124 1103 22-23 0.516881179519423 1103 24-25 0.6084497677500309 1103 26-27 0.4635763619055169 1103 28-29 0.5270576211018536 1103 30-31 0.6103629909677863 1103 32-33 0.571685184806789 1103 34-35 0.6876218632703583 1103 36-37 0.8615448910321533 1103 38-39 0.8067098948171534 1103 40-41 0.798511898200708 1103 42-43 0.7030170467258259 1103 44-45 0.6944047913972824 1103 46-47 0.7565999107346073 1103 48-49 0.786169047103968 1103 50-51 0.6957541716247917 1103 52-53 0.7180828704238778 1103 54-55 0.7855286369574799 1103 56-57 0.7891680366830585 1103 58-59 0.6788435347050026 1103 60-61 0.5619115754940012 1103 62-63 0.6661063729555039 1103 64-65 0.8756472478323261 1103 66-67 0.8794615929785294 1103 68-69 0.9171848858629161 1103 70-71 1.0911008952004195 1103 72-73 1.2552618610721105 1103 74-75 1.205874532750581 1103 76-77 0.9918361546468368 1103 78-79 0.9872625793037173 1103 80-81 0.7146477627852228 1103 82-83 0.9734130835060277 1103 84-85 1.0737769884253119 1103 86-87 1.1355875209226056 1103 88-89 1.4837066355093391 1103 90-91 1.4045549265336597 1103 92-93 1.2246729248318076 1103 94-95 1.062758297090621 1103 96-97 1.1956596811884665 1103 98-99 1.293016614548197 1103 100-101 1.8374276684578597 1104 1 0.5643620027367362 1104 2 0.45855276813004053 1104 3 0.4599526615339613 1104 4 0.28359084168392457 1104 5 0.3684737508222753 1104 6 0.32358857757749604 1104 7 0.3760129939713508 1104 8 0.5001743957888181 1104 9 0.37283545343640156 1104 10-11 0.4674048698349438 1104 12-13 0.3564711088502506 1104 14-15 0.5262108819770859 1104 16-17 0.7245816439655854 1104 18-19 0.6565481623269349 1104 20-21 0.562505453377149 1104 22-23 0.5838073617570245 1104 24-25 0.8618580722483031 1104 26-27 0.7100181150773679 1104 28-29 0.6716250951502971 1104 30-31 0.6087732178036163 1104 32-33 0.6731442390167146 1104 34-35 0.5932946814172197 1104 36-37 0.7374964481255688 1104 38-39 0.6761120478352254 1104 40-41 0.7443404256901331 1104 42-43 0.7499912874024872 1104 44-45 0.6851679594095899 1104 46-47 0.7735433940102681 1104 48-49 0.9486277168463744 1104 50-51 1.0606234526705478 1104 52-53 0.8174523437033869 1104 54-55 0.7349999941277972 1104 56-57 0.7311730347607224 1104 58-59 0.7785724851086897 1104 60-61 0.6504140745328257 1104 62-63 0.7811332856813848 1104 64-65 0.9381905004274884 1104 66-67 0.8860975022441906 1104 68-69 0.9214736209647967 1104 70-71 1.0936076233818923 1104 72-73 0.9615036911374766 1104 74-75 1.0721202074910643 1104 76-77 0.8993063584146164 1104 78-79 0.6862591190961034 1104 80-81 0.8748457635541591 1104 82-83 1.0132112380619738 1104 84-85 0.9250976343307329 1104 86-87 0.9370369634447115 1104 88-89 0.854842737008763 1104 90-91 0.9010082137308935 1104 92-93 0.941180037480791 1104 94-95 0.9817010114312623 1104 96-97 1.1008077011807806 1104 98-99 1.331972785251775 1104 100-101 1.0620489679580523 1105 1 0.1858322365280145 1105 2 0.15245009278658017 1105 3 0.24029732289814376 1105 4 0.15743032107654642 1105 5 0.13890035964367797 1105 6 0.16665678678453588 1105 7 0.11281704313982743 1105 8 0.22771356185293 1105 9 0.18272217312789252 1105 10-11 0.256380526619715 1105 12-13 0.2503322802168526 1105 14-15 0.36034440812796475 1105 16-17 0.3355855003165189 1105 18-19 0.34418012183525093 1105 20-21 0.2823017898437641 1105 22-23 0.14094763652202857 1105 24-25 0.1890128083292879 1105 26-27 0.09697762339262539 1105 28-29 0.30545734388493173 1105 30-31 0.1269378357838562 1105 32-33 0.22626426793933518 1105 34-35 0.41160150233793047 1105 36-37 0.15756152404748036 1105 38-39 0.07218700565638159 1105 40-41 0.10734355897525916 1105 42-43 -0.04340953207208287 1105 44-45 0.1115899888142664 1105 46-47 0.2453004439018116 1105 48-49 0.2379275481310188 1105 50-51 -0.034935718214008205 1105 52-53 0.009175650524305468 1105 54-55 -0.16092070965625282 1105 56-57 0.009711236737103945 1105 58-59 -0.06328217866570895 1105 60-61 0.1296126766056105 1105 62-63 0.12457387377489937 1105 64-65 0.2803633131534511 1105 66-67 0.30294734557994474 1105 68-69 0.20344638548637306 1105 70-71 0.33885226064388263 1105 72-73 0.3966531247359306 1105 74-75 0.3223262812437788 1105 76-77 0.09075731045124869 1105 78-79 0.006779726471371816 1105 80-81 0.17140517546064515 1105 82-83 0.029845366767684567 1105 84-85 0.19107377315936702 1105 86-87 0.46802515336999306 1105 88-89 0.3602838071461498 1105 90-91 0.4960371363628546 1105 92-93 0.774124120392333 1105 94-95 0.7969384180352606 1105 96-97 0.5751485061940329 1105 98-99 0.7499228937116413 1105 100-101 1.1956329641017014 1106 1 -0.11071035702884302 1106 2 -0.10953478120616467 1106 3 -0.02385908347261534 1106 4 0.03521624534376855 1106 5 0.09201160514592033 1106 6 0.1988678503917498 1106 7 0.1781299656254589 1106 8 -0.011326620835987455 1106 9 0.039033276663289485 1106 10-11 -0.44412604751943974 1106 12-13 -0.28806064572455625 1106 14-15 -0.28888539837215177 1106 16-17 0.006802072144033389 1106 18-19 0.03493172888595808 1106 20-21 -0.05913490064748572 1106 22-23 -0.2364384222401057 1106 24-25 -0.09078096614423004 1106 26-27 -0.10055418488675372 1106 28-29 -0.1587444778672804 1106 30-31 -0.08950031407056258 1106 32-33 0.05622102409027008 1106 34-35 -0.1182774989439821 1106 36-37 -0.26804714713814803 1106 38-39 -0.06616937783156374 1106 40-41 -0.131284499558042 1106 42-43 -0.27207305896757106 1106 44-45 -0.21603703999239343 1106 46-47 -0.04080536809952662 1106 48-49 -0.16802758754305103 1106 50-51 -0.14120946205257923 1106 52-53 -0.2297979852046339 1106 54-55 -0.4502738965551387 1106 56-57 -0.3612746389625201 1106 58-59 -0.17293989379334818 1106 60-61 -0.6134174049552783 1106 62-63 -0.4139731609159085 1106 64-65 -0.4126257894899865 1106 66-67 -0.4322687218916741 1106 68-69 -0.9709381718829455 1106 70-71 -0.5116692573739989 1106 72-73 -0.08502603018773058 1106 74-75 -0.055484744069747904 1106 76-77 -0.20745479455751692 1106 78-79 -0.2137516451665462 1106 80-81 -0.05161937219515167 1106 82-83 -0.03910487245112648 1106 84-85 -0.3416031191676545 1106 86-87 -0.17165458062896377 1106 88-89 -0.3702290247422191 1106 90-91 -0.4414478321966264 1106 92-93 0.0012523972463682753 1106 94-95 -0.19266449508710082 1106 96-97 0.14838693811415737 1106 98-99 -0.11358695640592487 1106 100-101 -0.2772812556994033 1107 1 0.3481188958494883 1107 2 0.3683802936492242 1107 3 0.3203949940459907 1107 4 0.21303117959310924 1107 5 0.20391901657377076 1107 6 0.20860911025378215 1107 7 0.11958919953277558 1107 8 0.2559669570935199 1107 9 0.33897883018167363 1107 10-11 0.3221121325358709 1107 12-13 0.24442253440463446 1107 14-15 0.37205624159224016 1107 16-17 0.5568865835408587 1107 18-19 0.45991493459021626 1107 20-21 0.5230821282568883 1107 22-23 0.5364384775613189 1107 24-25 0.5022259004777965 1107 26-27 0.4043880552392878 1107 28-29 0.4611991887792968 1107 30-31 0.4960318121952696 1107 32-33 0.3930685614383904 1107 34-35 0.5195454147270908 1107 36-37 0.5400835653378238 1107 38-39 0.41555297239230526 1107 40-41 0.3623387830760265 1107 42-43 0.2127813847860338 1107 44-45 0.3786532345474498 1107 46-47 0.3788337847177701 1107 48-49 0.044865543902574245 1107 50-51 0.18633329662220177 1107 52-53 0.1678477443838986 1107 54-55 0.38022761351064105 1107 56-57 0.2985712513511274 1107 58-59 0.33974988743182166 1107 60-61 0.5146068470307696 1107 62-63 0.5239169299381032 1107 64-65 0.6466822286921783 1107 66-67 0.4764436245016199 1107 68-69 0.38943501953338 1107 70-71 0.2988217498631798 1107 72-73 0.3194169845787478 1107 74-75 0.4671202074910674 1107 76-77 0.44669694858239595 1107 78-79 0.5767237442280972 1107 80-81 0.49328704009997537 1107 82-83 0.6669898371467973 1107 84-85 0.4468558180688511 1107 86-87 0.5666732417504718 1107 88-89 0.5756012727356214 1107 90-91 0.7187572457618678 1107 92-93 0.8027188149065765 1107 94-95 0.7171333709290906 1107 96-97 0.6715374911608336 1107 98-99 0.7831079489265349 1107 100-101 0.7990523705158381 1108 1 0.3459486164360257 1108 2 0.27992510471364085 1108 3 0.3123602695753718 1108 4 0.24959300853115707 1108 5 0.2386434871891936 1108 6 0.3031564119340615 1108 7 0.27110147356687264 1108 8 0.2992089449642137 1108 9 0.40521296637729165 1108 10-11 0.4377347121366242 1108 12-13 0.30935301567581774 1108 14-15 0.45852820471694855 1108 16-17 0.47179664336368177 1108 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-0.6054276627890722 2316 70-71 -0.9284397427092976 2316 72-73 -0.9538526612308296 2316 74-75 -0.8008080793615235 2316 76-77 -0.5684205118819747 2316 78-79 -0.5596173749278037 2316 80-81 -0.28798070833605394 2316 82-83 -0.6259587486578191 2316 84-85 -0.6980497760278332 2316 86-87 -0.8088705177416529 2316 88-89 -1.0953232656472771 2316 90-91 -1.1505522112359081 2316 92-93 -1.0637845486192532 2316 94-95 -1.08310863885648 2316 96-97 -1.1570431177656566 2316 98-99 -0.9696208402462361 2316 100-101 -0.9931347416479674 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2162.0 3 555.0 4 527.0 5 616.0 6 731.0 7 899.0 8 1108.0 9 1412.0 10 1786.0 11 2213.0 12 3057.0 13 3986.0 14 4806.0 15 5991.0 16 7744.0 17 10936.0 18 9351.0 19 6633.0 20 6373.0 21 6550.0 22 6961.0 23 7523.0 24 8219.0 25 9367.0 26 11057.0 27 12700.0 28 14833.0 29 17800.0 30 21553.0 31 26432.0 32 33341.0 33 44463.0 34 61142.0 35 91084.0 36 130524.0 37 176837.0 38 127346.0 39 13173.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.277176852352625 18.33051649540292 14.188299981972238 22.204006670272218 2 15.65172516309256 20.012564998625702 40.637000555328065 23.698709282953672 3 19.672517401860688 25.150905884095305 29.202139834216702 25.974436879827305 4 12.71291039249568 16.502771481789008 36.433652745674465 34.35066538004084 5 13.506655863466705 37.752919445086604 33.33284703970625 15.407577651740445 6 31.770330523961977 35.56489019204605 17.474080293838988 15.190698990152981 7 28.671888938635497 33.410646298235044 19.802217950746368 18.11524681238309 8 26.932022038901287 34.13673677514464 19.37558690186496 19.555654284089112 9 27.361867931280916 16.664030183802122 19.909035731635214 36.06506615328175 10-11 25.496589158247573 26.750223592768275 26.132916783092007 21.620270465892144 12-13 26.470487536700098 24.365272825342053 27.133741302406563 22.03049833555129 14-15 23.253323490110592 25.234010279286483 26.325721664925776 25.186944565677145 16-17 21.97317391848278 28.752624796706233 26.774076461457074 22.500124823353918 18-19 21.861113526522622 26.565649482338355 30.128328476018705 21.44490851512032 20-21 22.91363097980049 25.547910502948206 30.224977009669125 21.31348150758218 22-23 22.519391456650993 25.62477349050708 30.127473651966973 21.728361400874956 24-25 22.152831750396317 25.791950169049255 30.360909639314826 21.694308441239603 26-27 21.725504716862044 26.261041777119505 30.253782045348075 21.759671460670376 28-29 21.68275148589619 26.33803591285136 30.37893298235102 21.600279618901432 30-31 21.66107860760829 26.03794269658635 30.35707472962995 21.94390396617541 32-33 21.5768011993034 26.062911811702183 30.181141043824844 22.179145945169573 34-35 22.187953586415034 26.276213682929527 29.888577996244226 21.647254734411213 36-37 22.215938609099794 25.908010148035192 29.886771535681383 21.989279707183627 38-39 21.763543847132386 26.111294335668884 30.027382723195554 22.097779094003172 40-41 22.01219973917799 26.072160756103376 30.021174259952605 21.89446524476603 42-43 21.941206824989063 26.040238717566154 29.804527556846867 22.214026900597915 44-45 21.863524714274497 26.45571394923676 29.596731681602527 22.084029654886216 46-47 22.01574255565557 26.60884488226733 29.038817990370514 22.336594571706584 48-49 22.157327795309993 26.642969669675903 28.908952944561257 22.290749590452855 50-51 22.240199470814606 26.898500267508936 28.750761306879298 22.11053895479716 52-53 22.42081774046019 26.891656427198647 28.577172874934178 22.110352957406988 54-55 22.002696373131382 26.867159880121317 28.822815533980584 22.307328212766723 56-57 22.048264595550854 27.286416181891493 28.564674793579155 22.100644428978498 58-59 22.11692569276443 26.670659448255908 28.648880843508756 22.563534015470907 60-61 22.092609811933638 26.726297099311648 28.936668509904695 22.244424578850023 62-63 21.956779454763446 26.784154111705416 28.88646755935591 22.37259887417523 64-65 21.82988605271555 26.79031377983202 28.8728701828686 22.506929984583834 66-67 21.85044205010761 26.97453347255229 28.669554226420722 22.50547025091937 68-69 21.982310968948866 26.80888623705455 28.74899341203599 22.459809381960596 70-71 21.92799723592667 26.886390304205115 28.57708556287706 22.608526896991155 72-73 22.1101167507224 26.884243139436798 28.374082416497657 22.631557693343147 74-75 21.914603772293926 27.099474889417195 28.31609604425668 22.669825294032204 76-77 22.12959203587267 26.940448498089946 28.27625230204644 22.653707163990948 78-79 22.262060906082702 27.072404409121724 28.18915237623668 22.476382308558893 80-81 22.04202277724474 27.264224001848287 28.178947563213892 22.51480565769308 82-83 22.299773887607785 27.29213586483504 27.89027941031324 22.51781083724394 84-85 22.242784057549212 26.97709056385599 28.09825267392649 22.6818727046683 86-87 22.34212856244908 26.99636486790335 27.991638579388358 22.669867990259217 88-89 22.357981219340648 27.049612484842093 27.873495847771434 22.718910448045822 90-91 22.26501014756318 27.146442406829213 27.943157939612746 22.64538950599486 92-93 22.429236671918055 26.816734070995235 27.958672388042544 22.795356869044166 94-95 22.323642840113873 26.856605943315525 27.959162494565753 22.860588722004852 96-97 22.529765760975344 26.594130590738246 27.914498072454187 22.961605575832227 98-99 22.464643110923916 26.641514767253327 27.620408595914935 23.273433525907823 100-101 22.966150860086366 26.152982993714957 27.809749311599553 23.07111683459912 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1284.0 1 871.5 2 751.0 3 953.0 4 646.5 5 520.5 6 965.0 7 2011.0 8 3080.5 9 3366.0 10 2593.0 11 1757.5 12 1650.0 13 1725.5 14 1748.5 15 1758.5 16 1756.5 17 1778.0 18 1857.5 19 1957.0 20 2108.5 21 2313.5 22 2634.5 23 3104.0 24 3658.0 25 4363.5 26 5341.5 27 6425.0 28 7375.0 29 8440.5 30 9563.0 31 10780.0 32 12589.0 33 14071.0 34 15103.0 35 16299.0 36 17811.0 37 19528.0 38 20887.5 39 21742.0 40 22718.5 41 24647.0 42 26607.5 43 27925.0 44 29399.0 45 31284.0 46 32102.0 47 33030.0 48 34953.0 49 35241.5 50 34145.5 51 32683.0 52 31263.5 53 31044.0 54 30945.5 55 28863.5 56 26069.0 57 23834.5 58 21947.5 59 19490.0 60 16359.0 61 13391.0 62 10643.0 63 8114.5 64 6200.5 65 5057.0 66 3962.5 67 3104.5 68 2405.0 69 1843.0 70 1469.5 71 1064.0 72 855.0 73 756.0 74 567.5 75 423.5 76 305.5 77 189.0 78 123.0 79 84.5 80 47.0 81 28.5 82 20.0 83 14.0 84 11.0 85 8.0 86 6.5 87 6.5 88 5.0 89 2.5 90 1.5 91 0.0 92 1.0 93 1.0 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47903547015447545 2 0.04788112026122683 3 0.05696395572061647 4 0.06414051707124531 5 0.07826937223029586 6 0.108096955343847 7 0.06828946660207762 8 0.05146940093654125 9 0.0507965983099198 10-11 0.0743446902416707 12-13 0.10725595206057019 14-15 0.054384878985234225 16-17 0.05971123311265407 18-19 0.05881416294382547 20-21 0.07361582072949746 22-23 0.06156144033586307 24-25 0.08740827457523727 26-27 0.06442085149900426 28-29 0.06492545346897034 30-31 0.06795306528876689 32-33 0.06817733283097403 34-35 0.0707003426808045 36-37 0.06559825609559179 38-39 0.0396953549706658 40-41 0.04255476613380699 42-43 0.04059242513949441 44-45 0.022651021762922295 46-47 0.0290987136013779 48-49 0.028986579830274323 50-51 0.026127168667133143 52-53 0.025286165383856328 54-55 0.025117964727200962 56-57 0.02551043292606348 58-59 0.020520480111954356 60-61 0.027472773920376056 62-63 0.020071945027540054 64-65 0.021922152250749054 66-67 0.016932199436639934 68-69 0.01861420600319357 70-71 0.0396953549706658 72-73 0.03588280675314423 74-75 0.029771516227999353 76-77 0.02175395159409369 78-79 0.01575479484005239 80-81 0.017548935177709602 82-83 0.022426754220715144 84-85 0.01934307551536681 86-87 0.008690367260527118 88-89 0.03846188348852647 90-91 0.04989952814109119 92-93 0.03156565656565657 94-95 0.05096479896657517 96-97 0.04188196350718553 98-99 0.03089285393903511 100-101 0.1685931248542261 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 891792.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.85956335418254 #Duplication Level Percentage of deduplicated Percentage of total 1 84.31748210829988 54.6879507266843 2 9.679576011648711 12.556261471383106 3 2.5357849993378005 4.934097234514075 4 1.03566632182326 2.686914616563558 5 0.5884633675364784 1.9083738534173915 6 0.360113895848338 1.4014098025498063 7 0.2549621386665849 1.157571309003427 8 0.17926346619595118 0.9301560114261327 9 0.13839260052993874 0.8078475277639475 >10 0.8144416307242448 9.985406824518178 >50 0.06280963174983935 2.7509244135344364 >100 0.030967775701010697 3.5788210806715965 >500 8.65021640823723E-4 0.316431782164803 >1k 8.65021640823723E-4 0.9343714362008207 >5k 3.460086563294892E-4 1.36346190960444 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6814 0.764079516299765 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5337 0.5984579363797836 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3634 0.40749412419039416 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1360 0.152501928700863 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1236 0.13859734108401958 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1094 0.12267434558731183 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1003 0.11247017241688645 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.1213377110357572E-4 0.0 0.0 0.0 3 0.0 1.1213377110357572E-4 0.0 0.0 0.0 4 0.0 1.1213377110357572E-4 0.0 1.1213377110357572E-4 0.0 5 0.0 1.1213377110357572E-4 0.0 1.1213377110357572E-4 0.0 6 0.0 1.1213377110357572E-4 0.0 1.1213377110357572E-4 0.0 7 0.0 1.1213377110357572E-4 0.0 1.1213377110357572E-4 0.0 8 0.0 1.1213377110357572E-4 0.0 1.1213377110357572E-4 0.0 9 0.0 1.1213377110357572E-4 0.0 1.1213377110357572E-4 0.0 10-11 0.0 1.1213377110357572E-4 0.0 1.1213377110357572E-4 0.0 12-13 0.0 1.1213377110357572E-4 0.0 1.1213377110357572E-4 5.606688555178786E-5 14-15 0.0 1.1213377110357572E-4 0.0 1.1213377110357572E-4 1.6820065665536357E-4 16-17 0.0 1.1213377110357572E-4 0.0 1.1213377110357572E-4 2.2426754220715144E-4 18-19 0.0 1.1213377110357572E-4 0.0 2.2426754220715144E-4 2.2426754220715144E-4 20-21 0.0 1.1213377110357572E-4 0.0 2.2426754220715144E-4 4.485350844143029E-4 22-23 0.0 1.1213377110357572E-4 0.0 5.046019699660907E-4 5.606688555178786E-4 24-25 0.0 1.1213377110357572E-4 0.0 8.970701688286057E-4 5.606688555178786E-4 26-27 0.0 1.1213377110357572E-4 0.0 0.0016820065665536358 5.606688555178786E-4 28-29 0.0 1.1213377110357572E-4 0.0 0.005102086585212696 5.606688555178786E-4 30-31 0.0 1.1213377110357572E-4 0.0 0.014689524014568418 5.606688555178786E-4 32-33 0.0 1.6820065665536357E-4 0.0 0.034929669698763835 5.606688555178786E-4 34-35 5.606688555178786E-5 3.3640131331072714E-4 0.0 0.06313131313131312 5.606688555178786E-4 36-37 1.1213377110357572E-4 3.3640131331072714E-4 0.0 0.11168523601916142 5.606688555178786E-4 38-39 1.1213377110357572E-4 3.92468198862515E-4 0.0 0.18816046791180008 5.606688555178786E-4 40-41 1.1213377110357572E-4 4.485350844143029E-4 0.0 0.27226079623948185 5.606688555178786E-4 42-43 1.1213377110357572E-4 4.485350844143029E-4 0.0 0.350193767156467 5.606688555178786E-4 44-45 1.1213377110357572E-4 4.485350844143029E-4 0.0 0.44500287062454025 5.606688555178786E-4 46-47 1.1213377110357572E-4 4.485350844143029E-4 0.0 0.5547257656493891 5.606688555178786E-4 48-49 1.1213377110357572E-4 4.485350844143029E-4 0.0 0.6717373557959703 5.606688555178786E-4 50-51 1.1213377110357572E-4 4.485350844143029E-4 0.0 0.800803326336186 5.606688555178786E-4 52-53 1.1213377110357572E-4 6.728026266214543E-4 0.0 0.9206743276459084 5.606688555178786E-4 54-55 1.1213377110357572E-4 6.728026266214543E-4 0.0 1.0425637368354952 5.606688555178786E-4 56-57 1.1213377110357572E-4 6.728026266214543E-4 0.0 1.184356890395967 5.606688555178786E-4 58-59 1.1213377110357572E-4 7.8493639772503E-4 0.0 1.3462219889839782 5.606688555178786E-4 60-61 1.1213377110357572E-4 9.531370543803936E-4 0.0 1.5227766115865582 5.606688555178786E-4 62-63 1.1213377110357572E-4 0.0010092039399321815 0.0 1.6939488131761666 5.606688555178786E-4 64-65 1.1213377110357572E-4 0.0010092039399321815 0.0 1.8557578448786263 5.606688555178786E-4 66-67 2.2426754220715144E-4 0.0010092039399321815 0.0 2.0417877711394588 5.606688555178786E-4 68-69 2.2426754220715144E-4 0.0010092039399321815 0.0 2.2484503112833485 5.606688555178786E-4 70-71 2.2426754220715144E-4 0.0011213377110357572 0.0 2.473502789888225 5.606688555178786E-4 72-73 2.2426754220715144E-4 0.0011213377110357572 0.0 2.6983310009508945 6.728026266214543E-4 74-75 2.2426754220715144E-4 0.0011213377110357572 0.0 2.9229910113569084 7.849363977250301E-4 76-77 2.2426754220715144E-4 0.0011213377110357572 0.0 3.1521363726070657 7.849363977250301E-4 78-79 2.2426754220715144E-4 0.0011213377110357572 0.0 3.4042130900479037 7.849363977250301E-4 80-81 2.2426754220715144E-4 0.0011213377110357572 0.0 3.6872947951988806 8.410032832768179E-4 82-83 2.2426754220715144E-4 0.0011213377110357572 0.0 3.9713857042897898 8.970701688286057E-4 84-85 2.2426754220715144E-4 0.0011213377110357572 0.0 4.2521126002475915 8.970701688286057E-4 86-87 2.2426754220715144E-4 0.0011213377110357572 0.0 4.546519816280029 8.970701688286057E-4 88-89 2.2426754220715144E-4 0.0011213377110357572 0.0 4.855840823869244 8.970701688286057E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2990 0.0 49.778603 1 GTATCAA 5160 0.0 39.291496 1 TATAACG 85 1.6271611E-5 33.54334 2 ATCAACG 6425 0.0 31.581205 3 TATCAAC 6450 0.0 31.4588 2 TCAACGC 6525 0.0 31.097204 4 CAACGCA 6640 0.0 30.558622 5 ACCTCGT 80 4.4672092E-4 29.716513 8 AACGCAG 6890 0.0 29.38085 6 GGACCGA 290 0.0 26.217783 6 ACGCAGA 7790 0.0 26.06203 7 CGCAGAG 7825 0.0 25.702408 8 TAACGCA 150 1.0558342E-6 25.343857 4 GGACCGT 95 0.0012233728 25.010384 6 ACCGAGT 325 0.0 24.870436 8 GTACATG 5355 0.0 24.498108 1 GAGTACT 4425 0.0 24.438593 12-13 TACATGG 5285 0.0 24.18696 2 AGGACCG 400 0.0 23.759865 5 GCAGAGT 8380 0.0 23.432781 9 >>END_MODULE