FastQCFastQC Report
Thu 26 May 2016
SRR938245_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938245_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891792
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63970.7173197337495739No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54520.6113533200566948No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36640.4108581373235014No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14990.16808852288426002No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12650.1418492204460233No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10950.12278647935841541No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9950.11157310224805785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28550.047.9240841
GTATCAA49500.040.8857351
ATCAACG63550.031.7681623
TATCAAC63450.031.7469252
ACTGCGT600.003951531431.664958
TCAACGC64350.031.2958934
CAACGCA65800.030.6062415
AACGCAG68850.029.3883826
TACATGG57500.027.348132
ATAACGC700.00838821127.1444283
GTACATG59150.027.0671541
ACGCAGA77450.026.0637877
ACATGGG58000.026.0446113
CGCAGAG77800.025.8854838
TACCTGG7500.025.3376432
CATGGGA33400.024.4597514
GCAGAGT83000.023.9776129
TAGCCCT5700.023.3320644
TGGTATC14100.022.9116952
GTACCTG8700.022.3888631
Produced by FastQC (version 0.11.5)