FastQCFastQC Report
Thu 26 May 2016
SRR938244_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938244_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902377
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65590.7268580648664582No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54360.6024089709733293No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33350.3695794551501202No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13270.1470560530687285No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12810.14195840541148544No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10770.11935144623588588No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10330.1144754354333056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31100.045.6309781
GTATCAA51050.038.246531
TATCAAC64800.029.9439812
ATCAACG66450.029.0573123
TCAACGC67800.028.4085924
CAACGCA68350.028.2495755
AACGCAG70400.027.4269686
GTGTACG1501.0363256E-625.398181
GTACATG55250.024.995951
TACATGG54850.024.8845422
TGTACGA1151.2708624E-424.8129162
ACGCAGA80100.024.042227
CGCAGAG80950.023.7257568
ACATGGG56400.023.61043
GTGGTAT16350.023.5923481
TGGTATC16700.023.0670972
TATAGGG1652.4032506E-623.0584682
CATGGGA30650.022.8092634
ACTGCGT1050.002185245722.6379058
GAGTACT46350.022.4126612-13
Produced by FastQC (version 0.11.5)