##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938244_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 902377 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10358198402663 33.0 31.0 34.0 31.0 34.0 2 32.20534432947648 34.0 31.0 34.0 30.0 34.0 3 32.22667687673777 34.0 31.0 34.0 30.0 34.0 4 35.761022277828445 37.0 35.0 37.0 35.0 37.0 5 35.77379520976266 37.0 35.0 37.0 35.0 37.0 6 35.82072127281613 37.0 36.0 37.0 35.0 37.0 7 35.76457733297724 37.0 36.0 37.0 35.0 37.0 8 35.76418281937594 37.0 36.0 37.0 35.0 37.0 9 37.413009196821285 39.0 37.0 39.0 35.0 39.0 10-11 37.32547427516437 39.0 37.0 39.0 35.0 39.0 12-13 37.16783561637763 39.0 37.0 39.0 34.0 39.0 14-15 38.33955098589614 40.0 38.0 41.0 34.0 41.0 16-17 38.17414395535347 40.0 38.0 41.0 33.0 41.0 18-19 38.37572045830069 40.0 38.0 41.0 34.0 41.0 20-21 38.389832630929206 40.0 38.0 41.0 34.0 41.0 22-23 38.27103583092211 40.0 38.0 41.0 34.0 41.0 24-25 38.1490131064954 40.0 38.0 41.0 33.5 41.0 26-27 37.96783661374348 40.0 38.0 41.0 33.0 41.0 28-29 37.776673718412596 40.0 38.0 41.0 33.0 41.0 30-31 37.532048689184236 40.0 37.0 41.0 32.5 41.0 32-33 37.219958509580806 40.0 36.5 41.0 31.5 41.0 34-35 36.96769753661718 39.0 36.0 41.0 31.0 41.0 36-37 36.69642122970776 39.0 35.5 41.0 30.5 41.0 38-39 36.39465267842598 39.0 35.0 40.5 30.0 41.0 40-41 36.2629366661606 39.0 35.0 40.5 30.0 41.0 42-43 36.0070968120863 39.0 35.0 40.5 29.5 41.0 44-45 35.62708103154225 39.0 35.0 40.0 27.5 41.0 46-47 35.162203823900654 38.0 35.0 40.0 25.5 41.0 48-49 34.70407157983858 38.0 34.0 40.0 24.0 41.0 50-51 33.95951636621945 37.5 33.5 39.5 23.0 40.5 52-53 34.03271304565608 38.0 33.5 39.5 23.0 40.5 54-55 34.1068389376059 38.0 34.0 40.0 22.5 41.0 56-57 33.793187880453516 38.0 33.5 40.0 21.0 41.0 58-59 33.38341125715748 37.0 33.0 40.0 19.0 41.0 60-61 32.88123256687615 37.0 32.5 40.0 15.0 41.0 62-63 32.3381053595116 36.0 31.5 39.0 10.0 41.0 64-65 31.788425458538946 35.5 31.0 39.0 7.5 40.0 66-67 31.218196496586238 35.0 30.5 38.5 2.0 40.0 68-69 30.529535881344493 35.0 29.5 38.0 2.0 40.0 70-71 29.934460873891958 34.5 29.0 37.0 2.0 39.5 72-73 29.22709133765599 34.0 28.0 36.5 2.0 39.0 74-75 28.545927589023215 34.0 26.5 36.0 2.0 39.0 76-77 27.78165112807618 33.5 26.0 35.5 2.0 38.0 78-79 27.00392352641967 33.0 24.5 35.0 2.0 37.0 80-81 26.263572209841342 32.0 22.5 35.0 2.0 36.5 82-83 25.507577209968783 32.0 19.5 35.0 2.0 36.0 84-85 24.77092501249478 31.0 17.5 34.5 2.0 35.5 86-87 23.991405476868316 31.0 8.5 34.0 2.0 35.0 88-89 23.08074008978509 30.0 2.0 34.0 2.0 35.0 90-91 22.14756470965018 29.5 2.0 34.0 2.0 35.0 92-93 21.163114197281182 29.0 2.0 34.0 2.0 35.0 94-95 20.05068003727932 27.5 2.0 34.0 2.0 35.0 96-97 18.746257938755086 25.5 2.0 33.0 2.0 35.0 98-99 17.160168089390574 21.0 2.0 32.5 2.0 34.0 100-101 13.28239084107862 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15313227378398153 1101 2 0.23972090527909984 1101 3 0.14658609272807865 1101 4 -0.03624779220980656 1101 5 0.04037901963771162 1101 6 0.1381858863883494 1101 7 0.08243349078227169 1101 8 0.11938488255042756 1101 9 -0.008506121176345971 1101 10-11 0.3053625099586057 1101 12-13 0.15344976212072936 1101 14-15 0.154695469695028 1101 16-17 0.36754331988017697 1101 18-19 0.38175948363304 1101 20-21 0.4594458963220447 1101 22-23 0.34458117173107894 1101 24-25 0.3505763859773481 1101 26-27 0.40468506541501625 1101 28-29 0.441477925237173 1101 30-31 0.3742089976903884 1101 32-33 0.4216695945455271 1101 34-35 0.3819713423532036 1101 36-37 0.5002370237865037 1101 38-39 0.1950559611082312 1101 40-41 -0.0397612859003047 1101 42-43 0.17283164123647055 1101 44-45 -0.029589283003780054 1101 46-47 0.1494782733636555 1101 48-49 0.3094801098225659 1101 50-51 0.013058957588391706 1101 52-53 -0.05464405043968412 1101 54-55 -0.06605765960075871 1101 56-57 -0.15825450125804963 1101 58-59 0.17441112816086957 1101 60-61 0.19380212736138702 1101 62-63 0.011751563154469125 1101 64-65 -0.34018163729443174 1101 66-67 -0.37166715826051444 1101 68-69 -0.2306499826731674 1101 70-71 -0.1888884442791543 1101 72-73 -0.46829657438511063 1101 74-75 -0.45057826231327525 1101 76-77 -0.8448743793520563 1101 78-79 -0.9642950229962644 1101 80-81 -0.7711767851970066 1101 82-83 -0.9425957644733387 1101 84-85 -0.7816734962634762 1101 86-87 -0.5607732170465916 1101 88-89 -0.8290020532028812 1101 90-91 -1.210108868944289 1101 92-93 -1.2811840042070095 1101 94-95 -1.4448656804490234 1101 96-97 -1.4553345277868992 1101 98-99 -1.598637615365618 1101 100-101 -1.6571409918178013 1102 1 0.21680438925912426 1102 2 0.06972532323192127 1102 3 0.18241206884216155 1102 4 0.11207664486332902 1102 5 0.13236659142944518 1102 6 0.09847645731822041 1102 7 0.0966057040317807 1102 8 0.2730470757380843 1102 9 0.4176568505849687 1102 10-11 0.19447696390688662 1102 12-13 0.2022254097853704 1102 14-15 0.2736579026109496 1102 16-17 0.3236897062120434 1102 18-19 0.12358590878495335 1102 20-21 0.28582469594253723 1102 22-23 0.2907639880673685 1102 24-25 0.14411531103873187 1102 26-27 0.1382086362797068 1102 28-29 0.060118789128004835 1102 30-31 0.36278967602721224 1102 32-33 0.24078267241013407 1102 34-35 0.1576450801585665 1102 36-37 0.28163309687827365 1102 38-39 0.06269916434130351 1102 40-41 -0.031977722133312625 1102 42-43 0.1763609337121892 1102 44-45 0.20224677473380126 1102 46-47 0.2922962030095704 1102 48-49 0.16149752090220915 1102 50-51 0.2841459912589812 1102 52-53 0.1280506110799422 1102 54-55 -0.009184845944211872 1102 56-57 0.10902729894908703 1102 58-59 0.23590323740742747 1102 60-61 0.048468137370583975 1102 62-63 -2.3340132084825882E-4 1102 64-65 -0.4093790908153956 1102 66-67 -0.7764704145090207 1102 68-69 -0.47103151997589876 1102 70-71 -0.7890810959923762 1102 72-73 -0.8873799578127155 1102 74-75 -0.49156744919267936 1102 76-77 -0.9134229007863723 1102 78-79 -0.7381472605807318 1102 80-81 -0.9767622363713819 1102 82-83 -0.7361701780082051 1102 84-85 -0.8530038794665664 1102 86-87 -0.9783088684773524 1102 88-89 -0.9392060757127147 1102 90-91 -0.37971914756501945 1102 92-93 -0.5114643631475708 1102 94-95 -0.19195816051391645 1102 96-97 -0.4351951812093624 1102 98-99 -0.5645911327078927 1102 100-101 -1.029402766386326 1103 1 0.3680943300875299 1103 2 0.3937828042124778 1103 3 0.3280485861709863 1103 4 0.29311468374752536 1103 5 0.2915753826382428 1103 6 0.24095490195050928 1103 7 0.33070206920506706 1103 8 0.22897606982093066 1103 9 0.34734070519189686 1103 10-11 0.17656656661186787 1103 12-13 0.10228627200007168 1103 14-15 0.1266688916219394 1103 16-17 0.2769610502526163 1103 18-19 0.4395926712532514 1103 20-21 0.4123775277261359 1103 22-23 0.41174116473684563 1103 24-25 0.41973323157973397 1103 26-27 0.2685214004386225 1103 28-29 0.4157468533714521 1103 30-31 0.4414473260694791 1103 32-33 0.3050464086738742 1103 34-35 0.16269664397936623 1103 36-37 0.3523685153059901 1103 38-39 0.2962883443920248 1103 40-41 0.35242126349644565 1103 42-43 0.5249120748787135 1103 44-45 0.46532031657656603 1103 46-47 0.7348050787492113 1103 48-49 0.37443412276189036 1103 50-51 0.2555069380045367 1103 52-53 0.12681984184917638 1103 54-55 0.16199689539137552 1103 56-57 0.1833569692787549 1103 58-59 0.5941551393516278 1103 60-61 0.1991156437104067 1103 62-63 0.37234817095303896 1103 64-65 0.07482378323363648 1103 66-67 0.1445202870970661 1103 68-69 0.3135500522979804 1103 70-71 0.12444045937533232 1103 72-73 0.22622274717460655 1103 74-75 0.10485520507613089 1103 76-77 -0.11999940120902508 1103 78-79 -0.16190719295604694 1103 80-81 -0.03573772242379292 1103 82-83 -0.26410931579350105 1103 84-85 0.16282029635760864 1103 86-87 0.1894544451321103 1103 88-89 0.10548369605398378 1103 90-91 -0.043847634462736096 1103 92-93 -0.3708591222346378 1103 94-95 -0.37033516812169154 1103 96-97 -0.13370067571485933 1103 98-99 -0.3650205494449992 1103 100-101 -0.9438981171893701 1104 1 0.3008833836793201 1104 2 0.20540033149705295 1104 3 0.23326050993707526 1104 4 0.19717984093924912 1104 5 0.2834059739397574 1104 6 0.1860247830386328 1104 7 0.19248933097409093 1104 8 0.32359864678785755 1104 9 0.24065753203666418 1104 10-11 0.3253210868920462 1104 12-13 0.2521191438032986 1104 14-15 0.2925597533257971 1104 16-17 0.0945600077375488 1104 18-19 0.29398459176147895 1104 20-21 0.25140733714135166 1104 22-23 0.3223246473887471 1104 24-25 0.37901845718650407 1104 26-27 0.22801317434316815 1104 28-29 0.1877351250769479 1104 30-31 0.3257092579802148 1104 32-33 0.36192994019366154 1104 34-35 0.20737688506097385 1104 36-37 0.22791176339200803 1104 38-39 0.14527221798707046 1104 40-41 0.1970291800952424 1104 42-43 0.34064949139606426 1104 44-45 0.5448563973714826 1104 46-47 0.27314943450747364 1104 48-49 0.41182913823671896 1104 50-51 0.45222301554159827 1104 52-53 0.5694241748024282 1104 54-55 0.5177284477614705 1104 56-57 0.5077817941687996 1104 58-59 0.47672624213710435 1104 60-61 0.31856050443690265 1104 62-63 0.18712060946094056 1104 64-65 0.1993886960858866 1104 66-67 0.35689103151798207 1104 68-69 0.2546349195402051 1104 70-71 0.13625541295083465 1104 72-73 0.26221623145543305 1104 74-75 0.04175958689727466 1104 76-77 -0.09016718097771559 1104 78-79 -0.34629162038722683 1104 80-81 -0.4012135328163673 1104 82-83 -0.35423539495948475 1104 84-85 -0.503552076993099 1104 86-87 -0.6257291352555718 1104 88-89 -0.5700569457443585 1104 90-91 -0.6410113419069461 1104 92-93 -1.149778815995834 1104 94-95 -1.1620667204917865 1104 96-97 -1.5136882958484286 1104 98-99 -1.4346794515463195 1104 100-101 -1.4566878223530768 1105 1 0.10539665566892609 1105 2 0.27422577209731 1105 3 0.31607959392292884 1105 4 0.10719220312736866 1105 5 0.16942255871797585 1105 6 0.24047649663489779 1105 7 0.25210184772112854 1105 8 0.1545308870966764 1105 9 0.12158655236797955 1105 10-11 0.11389547026634972 1105 12-13 0.2530592509590974 1105 14-15 0.44760798353777176 1105 16-17 0.37826780551285566 1105 18-19 0.3069503678202494 1105 20-21 0.3521427547471774 1105 22-23 0.45398258223408305 1105 24-25 0.22937221275359576 1105 26-27 0.22105739157926507 1105 28-29 0.3658819585763311 1105 30-31 0.24872085873835914 1105 32-33 0.21248383946014116 1105 34-35 0.21483839370250024 1105 36-37 0.33843717532814566 1105 38-39 0.138286129552597 1105 40-41 0.260058029830887 1105 42-43 0.2559464048897908 1105 44-45 0.11985409287778737 1105 46-47 -0.06221374738589702 1105 48-49 -0.019656685654496187 1105 50-51 0.104100514970213 1105 52-53 0.10167698470631592 1105 54-55 0.1422538167645797 1105 56-57 0.09840458832637466 1105 58-59 0.256622470470127 1105 60-61 0.13714886630176437 1105 62-63 0.10078227948016405 1105 64-65 -0.11021719129571395 1105 66-67 -0.217065537930619 1105 68-69 -0.1658076442690195 1105 70-71 -0.09918744760141252 1105 72-73 -0.5025391575873002 1105 74-75 -0.5714327240490071 1105 76-77 -0.5820370534792332 1105 78-79 -0.5266602383602574 1105 80-81 -0.528331299389464 1105 82-83 -0.7115821840105561 1105 84-85 -0.9243557833766083 1105 86-87 -0.9615134396962368 1105 88-89 -1.438578319449892 1105 90-91 -1.1977523963674983 1105 92-93 -1.4141326549035185 1105 94-95 -1.359681790491571 1105 96-97 -1.6093241530127536 1105 98-99 -1.493228744350871 1105 100-101 -1.6975326686843868 1106 1 0.3613899599075765 1106 2 0.3330556650277572 1106 3 0.36501957405199903 1106 4 0.23082750049375989 1106 5 0.395664989689287 1106 6 0.2954859339005651 1106 7 0.4557788227856818 1106 8 0.33537195671038944 1106 9 0.5924762174945073 1106 10-11 0.4992540436592421 1106 12-13 0.5192961875344082 1106 14-15 0.5581150906671084 1106 16-17 0.09230442153429408 1106 18-19 0.4542030274780018 1106 20-21 0.44777157840552917 1106 22-23 0.5573540916556894 1106 24-25 0.4422686704339327 1106 26-27 0.49216811362261126 1106 28-29 0.46730836640855244 1106 30-31 0.4242376308803486 1106 32-33 0.6199402757322616 1106 34-35 0.4439283736892392 1106 36-37 0.7009619783981762 1106 38-39 0.6785446793167367 1106 40-41 0.618519684355789 1106 42-43 0.39736304807004785 1106 44-45 0.8136141101865562 1106 46-47 0.7917356700088476 1106 48-49 0.7515828557769524 1106 50-51 0.6999542387757529 1106 52-53 0.8315704845227003 1106 54-55 0.8797170569778672 1106 56-57 0.4976272593999411 1106 58-59 0.7796042763329041 1106 60-61 1.1026793601995664 1106 62-63 0.985538035073489 1106 64-65 0.7479519506959953 1106 66-67 0.5190917156684947 1106 68-69 0.3777219215598251 1106 70-71 0.14205852768234806 1106 72-73 0.18931419043571296 1106 74-75 0.20413614521568135 1106 76-77 -0.35155283492184 1106 78-79 0.2573722782154526 1106 80-81 0.19155129704003215 1106 82-83 0.42318851747862496 1106 84-85 0.4681746848996582 1106 86-87 0.04893516492645844 1106 88-89 -0.15507744974109627 1106 90-91 -0.3493621405222278 1106 92-93 -0.3416692581141838 1106 94-95 -0.404518054643912 1106 96-97 -0.2711255747233068 1106 98-99 -0.05376531624632008 1106 100-101 -0.13059000114089336 1107 1 0.039250024967358854 1107 2 0.020043440128283407 1107 3 0.20234863095379296 1107 4 0.1880685574600207 1107 5 0.051504924360884274 1107 6 0.17186160444342136 1107 7 0.15022617728089926 1107 8 0.14260616386481217 1107 9 0.1592457656486843 1107 10-11 0.20244565304268036 1107 12-13 0.0270935566694277 1107 14-15 0.2086309755150566 1107 16-17 0.41009136821052294 1107 18-19 0.28637457900067176 1107 20-21 0.3350471799130261 1107 22-23 0.4000904706034021 1107 24-25 0.29122629024520563 1107 26-27 0.2616726170381156 1107 28-29 0.272990621097982 1107 30-31 0.07457824113142664 1107 32-33 0.08128584739606737 1107 34-35 0.26255811584083233 1107 36-37 0.2974140444894573 1107 38-39 0.24029371832843083 1107 40-41 0.1628359522559606 1107 42-43 0.20042961481004795 1107 44-45 0.21911222581675105 1107 46-47 0.2491708049770125 1107 48-49 0.18228424964650003 1107 50-51 0.08917464960328658 1107 52-53 0.10929006128490926 1107 54-55 -0.061577369424263395 1107 56-57 -0.12610653273647188 1107 58-59 -0.24219894334727599 1107 60-61 -0.2701854460044828 1107 62-63 0.014221286343392592 1107 64-65 -0.02550999792773112 1107 66-67 0.07473434889733355 1107 68-69 -0.2072560381632833 1107 70-71 -0.1913063685094265 1107 72-73 -0.22025822013394603 1107 74-75 -0.23289729529706094 1107 76-77 -0.6987066709507772 1107 78-79 -0.792953020691531 1107 80-81 -0.8250937457108485 1107 82-83 -1.09970328504264 1107 84-85 -1.0171707470817104 1107 86-87 -0.6575215214300627 1107 88-89 -0.8432256635519302 1107 90-91 -0.822024675812191 1107 92-93 -1.2466328198014374 1107 94-95 -1.2980169116655063 1107 96-97 -1.4207590426352965 1107 98-99 -1.857941283882976 1107 100-101 -1.660595534714373 1108 1 0.08340550833526095 1108 2 0.07809872997855649 1108 3 -0.11567692095028548 1108 4 -0.10462607297812809 1108 5 -0.0958462833091076 1108 6 -0.15103819256826512 1108 7 -0.032381228162215336 1108 8 -0.10530791809448203 1108 9 -0.03141803455778103 1108 10-11 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13 5106.0 14 5935.0 15 7758.0 16 9687.0 17 12616.0 18 12116.0 19 9443.0 20 9456.0 21 10285.0 22 11268.0 23 12516.0 24 14166.0 25 15938.0 26 18242.0 27 20918.0 28 24009.0 29 28171.0 30 33488.0 31 39362.0 32 47970.0 33 59438.0 34 76104.0 35 99670.0 36 118169.0 37 121510.0 38 57418.0 39 3148.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.212793110719005 18.35303126144422 14.177958295879526 22.256217331957252 2 15.72822165205648 19.990087395025068 40.60996893163407 23.67172202128438 3 19.671698113207547 25.11287458379578 29.269145394006657 25.94628190899001 4 12.734600664255696 16.5331534168776 36.374158167426614 34.358087751440095 5 13.583328521747628 37.757327304786564 33.23291924086502 15.426424932600789 6 31.726467482369557 35.516054778457715 17.550825974129804 15.206651765042922 7 28.808560471092314 33.352203666394715 19.759236750528647 18.07999911198433 8 27.038290586158254 34.217331902020106 19.217708952509383 19.52666855931226 9 27.296007243016057 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30.147393743297325 22.18124098796583 34-35 22.25612918218964 26.30693859530897 29.83034133067994 21.606590891821455 36-37 22.185378125254772 25.955843653100537 29.801235987272978 22.057542234371716 38-39 21.82221783631729 26.11708444259152 29.915044035840726 22.145653685250462 40-41 22.037618684593525 26.07825906161203 29.97064245616467 21.913479797629773 42-43 21.914709349325285 26.060200468625073 29.766286273809666 22.258803908239972 44-45 21.892369050489595 26.432857455648033 29.575922861807 22.098850632055363 46-47 22.164517523921457 26.567950238937364 28.98094044860352 22.28659178853766 48-49 22.183437823934725 26.622486820880383 28.851545740275725 22.342529614909175 50-51 22.286352944176706 26.973008364097417 28.615336340719853 22.125302351006027 52-53 22.43660623675688 26.945820370804537 28.429936388693516 22.18763700374507 54-55 22.00281070952398 26.91207246862899 28.688640464792677 22.396476357054354 56-57 22.11639132466228 27.303336971058744 28.394599169401424 22.185672534877547 58-59 22.29046745489633 26.73915851576875 28.417015515672844 22.553358513662076 60-61 22.136710634552152 26.780278086343788 28.75905465720348 22.323956621900575 62-63 22.12398456803983 26.823481016903862 28.69371084977234 22.358823565283966 64-65 21.951000488908647 26.74646261844394 28.693837866362752 22.608699026284658 66-67 21.892895806529754 26.969893332609026 28.534067545851787 22.603143315009433 68-69 22.11481257031143 26.79793499938733 28.55199899311537 22.53525343718587 70-71 21.96722147624217 26.863653449329227 28.408719207070632 22.760405867357967 72-73 22.210296503189454 26.900005433101338 28.158489109958207 22.731208953751004 74-75 21.982738788499994 27.149072940232127 28.142571021365033 22.725617249902854 76-77 22.23113902414963 26.912312243742015 28.105716569105276 22.75083216300308 78-79 22.348461126207436 26.945324575925035 28.10946280050478 22.596751497362746 80-81 22.128119705347846 27.204612101001107 28.014345753498453 22.652922440152594 82-83 22.379697756338494 27.211073991394237 27.73097786930855 22.678250382958726 84-85 22.392442875431588 26.95891686387089 27.791829925902938 22.856810334794584 86-87 22.46121342550589 26.863094047155204 27.803183178822554 22.87250934851635 88-89 22.526694456180504 26.818045219505 27.729521312871558 22.925739011442936 90-91 22.37696524439425 27.012340364630425 27.708794908975527 22.9018994819998 92-93 22.58633731053926 26.67112521876851 27.69731311235773 23.045224358334497 94-95 22.45613315159225 26.68067063790899 27.636419493616692 23.22677671688207 96-97 22.640739424338925 26.463896838128104 27.61629470511357 23.279069032419404 98-99 22.626785801409568 26.479869954182355 27.20012286047574 23.693221383932332 100-101 23.632391069269698 25.430976369328896 27.28199289709442 23.654639664306984 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1237.0 1 836.0 2 726.0 3 919.0 4 636.5 5 518.0 6 923.5 7 1884.0 8 2916.5 9 3187.5 10 2477.5 11 1703.0 12 1545.0 13 1644.0 14 1726.0 15 1770.5 16 1809.5 17 1822.5 18 1871.0 19 2002.0 20 2153.0 21 2347.5 22 2728.0 23 3289.5 24 3836.0 25 4493.0 26 5526.5 27 6650.0 28 7525.0 29 8550.5 30 9706.0 31 11013.5 32 12789.0 33 14173.0 34 15257.5 35 16431.5 36 18111.5 37 19829.0 38 21031.0 39 21913.0 40 22819.5 41 24685.0 42 26504.0 43 27822.0 44 29287.5 45 30992.0 46 32045.0 47 33169.5 48 34926.0 49 35325.0 50 34310.0 51 32969.5 52 31800.0 53 31346.0 54 30965.5 55 29111.5 56 26538.5 57 24247.5 58 22082.0 59 19852.0 60 17067.5 61 14084.0 62 11321.0 63 8732.0 64 6789.5 65 5525.0 66 4361.5 67 3432.0 68 2712.0 69 2063.5 70 1563.5 71 1193.5 72 959.0 73 791.0 74 608.0 75 481.5 76 362.0 77 226.5 78 141.5 79 81.5 80 55.5 81 38.0 82 24.5 83 17.5 84 12.0 85 12.5 86 8.0 87 4.0 88 5.5 89 4.0 90 2.5 91 3.0 92 2.0 93 1.0 94 0.5 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14040694742884627 2 0.05485512152902833 3 0.152596974435297 4 0.13497684448960912 5 0.19648107165851966 6 0.19991644290579214 7 0.1653410935784046 8 0.07103461191940841 9 0.12134617792785056 10-11 0.10677355473377534 12-13 0.1503251966750039 14-15 0.13846762495054726 16-17 0.1379689420275561 18-19 0.13625125640391988 20-21 0.13807976045488748 22-23 0.19398765704356383 24-25 0.21110910406626054 26-27 0.14932783082902157 28-29 0.12882642177271805 30-31 0.10200836235852644 32-33 0.127607419072073 34-35 0.09868380953858531 36-37 0.0920347038987031 38-39 0.055187576811022446 40-41 0.06255700222855858 42-43 0.06538287212550851 44-45 0.039839224625627646 46-47 0.051752205563749966 48-49 0.04233263924058348 50-51 0.05391316489671169 52-53 0.02853574503782787 54-55 0.05202925163207839 56-57 0.028424926610496497 58-59 0.0534698911873862 60-61 0.03934054170263648 62-63 0.0534698911873862 64-65 0.0407811812579443 66-67 0.04072577204427861 68-69 0.06488418920251735 70-71 0.0560741242296734 72-73 0.055187576811022446 74-75 0.04571260127419028 76-77 0.023271869739587777 78-79 0.02299482367125935 80-81 0.025322010645218128 82-83 0.02116631962029174 84-85 0.020168953774309408 86-87 0.025322010645218128 88-89 0.06626941954415948 90-91 0.11369970644198599 92-93 0.0818948177978827 94-95 0.08067581509723763 96-97 0.07186575012439368 98-99 0.05995276918627137 100-101 0.13259424830198463 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 902377.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.27468362583353 #Duplication Level Percentage of deduplicated Percentage of total 1 84.65131376092646 55.25587724255643 2 9.469538754115172 12.362422925148755 3 2.4881978951930654 4.872489912215768 4 1.0549871593184372 2.754558122153113 5 0.5625532303404902 1.8360242066583075 6 0.3339559335961989 1.3079320746277054 7 0.23389884925882082 1.0687371370071241 8 0.16796129816673844 0.8770896479374516 9 0.12934488861908436 0.7598652014906466 >10 0.8124026255893004 9.988343168990037 >50 0.06248926179022191 2.7599156024773928 >100 0.03132448470472994 3.635911218784084 >500 8.466076588664836E-4 0.30504649992419963 >1k 8.466076588664836E-4 0.8900505303947752 >5k 3.3864306354659346E-4 1.3257365096343385 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6559 0.7268580648664582 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5436 0.6024089709733293 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3335 0.3695794551501202 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1327 0.1470560530687285 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1281 0.14195840541148544 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1077 0.11935144623588588 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1033 0.1144754354333056 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.1081842733137037E-4 14-15 0.0 0.0 0.0 0.0 2.2163685466274074E-4 16-17 0.0 0.0 0.0 0.0 3.324552819941111E-4 18-19 1.1081842733137037E-4 0.0 0.0 5.5409213665685186E-5 3.878644956597963E-4 20-21 1.1081842733137037E-4 2.2163685466274074E-4 0.0 1.6622764099705556E-4 4.432737093254815E-4 22-23 1.1081842733137037E-4 2.2163685466274074E-4 0.0 8.311382049852778E-4 4.432737093254815E-4 24-25 1.1081842733137037E-4 2.2163685466274074E-4 0.0 0.0014406395553078148 4.432737093254815E-4 26-27 1.1081842733137037E-4 2.2163685466274074E-4 0.0 0.0023271869739587775 4.432737093254815E-4 28-29 1.1081842733137037E-4 2.2163685466274074E-4 0.0 0.005430102939237148 4.432737093254815E-4 30-31 1.1081842733137037E-4 2.2163685466274074E-4 0.0 0.01656735488603987 4.432737093254815E-4 32-33 1.1081842733137037E-4 3.324552819941111E-4 0.0 0.03557271517336989 4.432737093254815E-4 34-35 1.1081842733137037E-4 4.432737093254815E-4 0.0 0.06571532740750263 4.432737093254815E-4 36-37 1.1081842733137037E-4 4.432737093254815E-4 0.0 0.11275774980966935 4.432737093254815E-4 38-39 1.1081842733137037E-4 4.432737093254815E-4 0.0 0.1862303671303679 6.649105639882222E-4 40-41 1.1081842733137037E-4 4.432737093254815E-4 0.0 0.26657372694561143 6.649105639882222E-4 42-43 1.1081842733137037E-4 4.432737093254815E-4 0.0 0.3444236721458991 6.649105639882222E-4 44-45 1.1081842733137037E-4 4.432737093254815E-4 0.0 0.43590428390794533 6.649105639882222E-4 46-47 2.2163685466274074E-4 4.986829229911666E-4 0.0 0.5447833887610167 6.649105639882222E-4 48-49 2.2163685466274074E-4 5.540921366568518E-4 0.0 0.6604778268949674 6.649105639882222E-4 50-51 2.2163685466274074E-4 6.649105639882222E-4 0.0 0.7826551430278033 7.757289913195925E-4 52-53 2.2163685466274074E-4 6.649105639882222E-4 0.0 0.9056081881519586 7.757289913195925E-4 54-55 2.2163685466274074E-4 6.649105639882222E-4 0.0 1.0305559649680789 7.757289913195925E-4 56-57 2.2163685466274074E-4 7.203197776539074E-4 0.0 1.1754510587038456 7.757289913195925E-4 58-59 2.2163685466274074E-4 7.757289913195925E-4 0.0 1.3354174585566787 7.757289913195925E-4 60-61 2.2163685466274074E-4 7.757289913195925E-4 0.0 1.505689972151329 8.86547418650963E-4 62-63 2.2163685466274074E-4 7.757289913195925E-4 0.0 1.6765719870963023 8.86547418650963E-4 64-65 2.2163685466274074E-4 9.419566323166481E-4 0.0 1.8418022622473758 8.86547418650963E-4 66-67 2.2163685466274074E-4 0.0012190027006450741 0.0 2.0326315941119955 8.86547418650963E-4 68-69 2.2163685466274074E-4 0.0013298211279764445 0.0 2.2408594190676405 8.86547418650963E-4 70-71 2.2163685466274074E-4 0.0013298211279764445 0.0 2.476902669283459 8.86547418650963E-4 72-73 2.2163685466274074E-4 0.0013298211279764445 0.0 2.70341553474878 8.86547418650963E-4 74-75 2.2163685466274074E-4 0.0013298211279764445 0.0 2.929762172573104 9.973658459823333E-4 76-77 2.2163685466274074E-4 0.0013298211279764445 0.0 3.163755281883293 0.0011081842733137036 78-79 2.2163685466274074E-4 0.0013298211279764445 0.0 3.420299941155415 0.0011081842733137036 80-81 3.324552819941111E-4 0.0013298211279764445 0.0 3.709314399635629 0.0011081842733137036 82-83 3.324552819941111E-4 0.0013298211279764445 0.0 3.9906823866299783 0.0012190027006450741 84-85 3.324552819941111E-4 0.0013298211279764445 0.0 4.270720552496352 0.0012190027006450741 86-87 3.324552819941111E-4 0.0013298211279764445 0.0 4.569597851009057 0.0012190027006450741 88-89 3.324552819941111E-4 0.0013298211279764445 0.0 4.8720767484100325 0.0012190027006450741 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3110 0.0 45.630978 1 GTATCAA 5105 0.0 38.24653 1 TATCAAC 6480 0.0 29.943981 2 ATCAACG 6645 0.0 29.057312 3 TCAACGC 6780 0.0 28.408592 4 CAACGCA 6835 0.0 28.249575 5 AACGCAG 7040 0.0 27.426968 6 GTGTACG 150 1.0363256E-6 25.39818 1 GTACATG 5525 0.0 24.99595 1 TACATGG 5485 0.0 24.884542 2 TGTACGA 115 1.2708624E-4 24.812916 2 ACGCAGA 8010 0.0 24.04222 7 CGCAGAG 8095 0.0 23.725756 8 ACATGGG 5640 0.0 23.6104 3 GTGGTAT 1635 0.0 23.592348 1 TGGTATC 1670 0.0 23.067097 2 TATAGGG 165 2.4032506E-6 23.058468 2 CATGGGA 3065 0.0 22.809263 4 ACTGCGT 105 0.0021852457 22.637905 8 GAGTACT 4635 0.0 22.41266 12-13 >>END_MODULE