FastQCFastQC Report
Thu 26 May 2016
SRR938244_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938244_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902377
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64240.7118975771767232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52790.5850104778823042No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35090.3888618615057786No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13990.15503497983658715No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12400.13741484989089925No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11240.1245599123204603No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9750.10804796664808611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30450.045.3997271
GTATCAA50300.039.6669581
TATCAAC64900.030.743422
ATCAACG66500.030.1432613
TCAACGC67200.029.7536374
CAACGCA67950.029.3553375
AACGCAG69850.028.5568396
GTATACG700.00839688727.1387084
TGCACCG1754.965841E-927.1387085
GCACCGT1955.366019E-1026.790786
ACGCAGA80300.024.7813937
GTACATG55150.024.722021
TACATGG54250.024.6943132
CGCAGAG81000.024.5672328
GAGTACT45800.023.79953812-13
ACATGGG55150.023.599593
GCAGAGT86500.022.8953449
AGTACTT46900.022.02610612-13
GTACTTT48850.022.02190814-15
GTATAGG2257.138078E-821.1137351
Produced by FastQC (version 0.11.5)