FastQCFastQC Report
Thu 26 May 2016
SRR938243_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938243_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2401748
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121290.5050071864325484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99000.4121997811593889No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60500.25189986626407096No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28050.11678993799516019No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28030.11670666531209768No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC25400.10575630748937857No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24030.10005212869959713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48200.059.973061
GTATCAA91650.044.396341
TGGTATC18150.036.9197922
TATCAAC115900.034.9359782
ATCAACG117400.034.2467233
TCAACGC118400.033.917344
GTGGTAT20500.033.5072861
CAACGCA120150.033.423335
AACGCAG124600.032.299196
ACGCAGA140550.028.458837
CGCAGAG142300.027.9085458
GCAGAGT154150.025.6079719
GAGTACT107200.022.70856312-13
GTGTAGC16400.022.6872231
GTACATG82050.022.5571251
CAGAGTA149550.022.4560110-11
TACATGG81400.022.0690752
GTACTTT112250.021.58338714-15
TAAGGTG16050.020.7271585
AGAGTAC145850.020.6807810-11
Produced by FastQC (version 0.11.5)