FastQCFastQC Report
Thu 26 May 2016
SRR938243_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938243_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2401748
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114030.47477920248085975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98940.4119499631102014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60580.25223295699632103No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27490.11445830286941011No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC27270.11354230335572257No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26230.10921212383647244No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25820.10750503383369113No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25360.10558976212325355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50350.061.3857881
GTATCAA93150.043.2368971
TGGTATC17850.041.0238572
GTGGTAT21650.035.141161
ATCAACG117650.033.9458733
TATCAAC119050.033.67072
TCAACGC119900.033.3081674
CAACGCA121550.032.8560185
AACGCAG126400.031.6758176
ACGCAGA143750.027.7205267
CGCAGAG144700.027.4400628
GCAGAGT160050.024.3632289
GAGTACT111550.022.8852812-13
TAAGGTG15300.022.6841245
GTAAGGT14900.022.3358444
CAGAGTA154800.021.98328610-11
GTACTTT115900.021.96487214-15
GTACATG80650.021.1072961
AGAGTAC150750.020.5417610-11
TACATGG80800.020.1853712
Produced by FastQC (version 0.11.5)