##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938243_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2401748 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49016008340592 33.0 31.0 34.0 31.0 34.0 2 32.714603280610625 34.0 31.0 34.0 31.0 34.0 3 32.77836725584866 34.0 31.0 34.0 31.0 34.0 4 36.23434973194523 37.0 35.0 37.0 35.0 37.0 5 36.16205113942012 37.0 35.0 37.0 35.0 37.0 6 36.220296633951605 37.0 37.0 37.0 35.0 37.0 7 36.193873378889045 37.0 37.0 37.0 35.0 37.0 8 36.18479082734741 37.0 37.0 37.0 35.0 37.0 9 37.88045290346864 39.0 38.0 39.0 35.0 39.0 10-11 37.85198072403932 39.0 38.0 39.0 35.0 39.0 12-13 37.77281213516156 39.0 38.0 39.0 35.0 39.0 14-15 39.014461966867465 40.0 38.0 41.0 36.0 41.0 16-17 38.927623131152814 40.0 38.0 41.0 35.5 41.0 18-19 39.184993804512374 40.0 39.0 41.0 36.0 41.0 20-21 39.16009485591327 40.0 39.0 41.0 36.0 41.0 22-23 39.05796278377249 40.0 39.0 41.0 35.5 41.0 24-25 38.95346077107173 40.0 38.0 41.0 35.0 41.0 26-27 38.79036185311698 40.0 38.0 41.0 35.0 41.0 28-29 38.66281620719576 40.0 38.0 41.0 34.5 41.0 30-31 38.41114534081011 40.0 38.0 41.0 34.0 41.0 32-33 38.1677959136429 40.0 38.0 41.0 34.0 41.0 34-35 38.0595237302165 40.0 38.0 41.0 34.0 41.0 36-37 37.99762423035223 40.0 38.0 41.0 33.0 41.0 38-39 37.79760803381537 40.0 37.0 41.0 33.0 41.0 40-41 37.57134782666624 40.0 37.0 41.0 33.0 41.0 42-43 37.30470099277693 40.0 37.0 41.0 32.0 41.0 44-45 37.01863351192548 40.0 36.0 41.0 31.0 41.0 46-47 36.63111929311485 39.5 36.0 41.0 30.5 41.0 48-49 36.195148283666725 39.0 35.5 41.0 30.0 41.0 50-51 35.80506073076776 39.0 35.0 40.0 28.5 41.0 52-53 35.270512351837084 38.0 35.0 40.0 27.0 41.0 54-55 34.8984903911651 38.0 34.0 40.0 26.0 41.0 56-57 34.518973680835785 38.0 34.0 40.0 25.0 41.0 58-59 34.02188718383444 37.5 33.5 40.0 23.5 41.0 60-61 33.55077489395224 37.0 33.0 40.0 22.5 41.0 62-63 33.00107109488589 36.0 32.0 39.0 20.5 40.5 64-65 32.43976116561771 36.0 31.5 39.0 18.5 40.0 66-67 31.883692210839772 35.0 31.0 38.5 14.5 40.0 68-69 31.26599480878094 35.0 30.5 38.0 8.0 40.0 70-71 30.650334672913228 35.0 29.5 37.5 7.0 39.5 72-73 29.986848328800523 34.0 29.0 37.0 3.5 39.0 74-75 29.332356683548817 34.0 28.0 36.0 2.0 39.0 76-77 28.040008776940795 32.5 26.5 35.0 2.0 37.0 78-79 28.694657391200074 34.0 28.0 35.0 2.0 37.0 80-81 28.416012420953408 34.0 27.5 35.0 2.0 37.0 82-83 27.852775978162573 33.5 26.5 35.0 2.0 36.5 84-85 27.19495363377007 33.0 26.0 35.0 2.0 36.0 86-87 26.48738772760506 32.5 24.5 35.0 2.0 35.5 88-89 25.683954769609468 32.0 21.5 34.5 2.0 35.0 90-91 25.026730531263063 31.5 19.0 34.0 2.0 35.0 92-93 24.09366886117944 31.0 11.0 34.0 2.0 35.0 94-95 23.200092599223566 31.0 2.0 34.0 2.0 35.0 96-97 22.150956719855706 30.0 2.0 34.0 2.0 35.0 98-99 20.74453377290207 29.0 2.0 34.0 2.0 35.0 100-101 16.887765077768357 21.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2020505652499054 1101 2 0.14591641977383318 1101 3 0.19493150212110777 1101 4 0.11178116641938374 1101 5 0.012920203912834438 1101 6 0.09937622675483482 1101 7 0.13202337397439834 1101 8 0.10528450104439457 1101 9 0.02869024708214596 1101 10-11 0.1679628032059597 1101 12-13 0.2860201920819634 1101 14-15 0.3847472854902705 1101 16-17 0.33034241058359726 1101 18-19 0.30590276733194344 1101 20-21 0.23673248237339095 1101 22-23 0.3241193305268908 1101 24-25 0.3802912947799584 1101 26-27 0.35350635554561904 1101 28-29 0.29058903878986797 1101 30-31 0.388333659126765 1101 32-33 0.36617847841088036 1101 34-35 0.2548643051622079 1101 36-37 0.37987110088078424 1101 38-39 0.48499840570593733 1101 40-41 0.4775468513715566 1101 42-43 0.5878652837197009 1101 44-45 0.6500553422599893 1101 46-47 0.661351063542682 1101 48-49 0.7416647938049508 1101 50-51 0.7057093071672611 1101 52-53 0.669429649642538 1101 54-55 0.9162824498027717 1101 56-57 0.8496800413625252 1101 58-59 1.00446665129882 1101 60-61 0.9958557850296046 1101 62-63 1.019692272100059 1101 64-65 1.1884910078971345 1101 66-67 1.3128430643373932 1101 68-69 1.5656789201760688 1101 70-71 1.7507590591516902 1101 72-73 1.896436658649307 1101 74-75 2.0089048593786245 1101 76-77 1.9179513958863836 1101 78-79 2.1035766076643263 1101 80-81 2.296145179061437 1101 82-83 2.236309200588831 1101 84-85 2.349136056881843 1101 86-87 2.6234641282372415 1101 88-89 2.9144990574465126 1101 90-91 3.0496964920746876 1101 92-93 3.2541273804248405 1101 94-95 3.7679476293417657 1101 96-97 3.9921464411201413 1101 98-99 4.23395686364422 1101 100-101 4.098608551045007 1102 1 0.1616808820230773 1102 2 0.1385050858318877 1102 3 0.1705379062989394 1102 4 0.11447084257844153 1102 5 0.0067359120142569395 1102 6 0.03307178289936985 1102 7 0.06568457294171992 1102 8 0.11663709402964173 1102 9 0.20501128054603157 1102 10-11 0.23971040556580903 1102 12-13 0.28501892577457255 1102 14-15 0.39201873663927955 1102 16-17 0.40070345543443864 1102 18-19 0.4164666176917109 1102 20-21 0.2596757145002968 1102 22-23 0.2758033230272048 1102 24-25 0.38270365943722595 1102 26-27 0.3755612092331262 1102 28-29 0.3449838125723943 1102 30-31 0.3639400633046037 1102 32-33 0.27686454215815814 1102 34-35 0.36204888173453753 1102 36-37 0.45786680132545854 1102 38-39 0.5723736155301324 1102 40-41 0.6509229839313662 1102 42-43 0.6880238175517022 1102 44-45 0.600765063377871 1102 46-47 0.6170523916929938 1102 48-49 0.5904220456234199 1102 50-51 0.8025744601548652 1102 52-53 0.9514579010585464 1102 54-55 0.9122111243028783 1102 56-57 1.102362355269328 1102 58-59 1.1124267184425563 1102 60-61 0.9918703524727448 1102 62-63 1.2673756167221768 1102 64-65 1.168082844631833 1102 66-67 1.2664191556685864 1102 68-69 1.706086887114651 1102 70-71 1.7658369517805994 1102 72-73 1.785821272523716 1102 74-75 1.8467635237679048 1102 76-77 1.8888852239630687 1102 78-79 1.893187978908351 1102 80-81 2.0523122923714077 1102 82-83 2.0945637578229785 1102 84-85 2.4208100367190895 1102 86-87 2.3924538309004113 1102 88-89 2.6526311068030495 1102 90-91 2.699486422476866 1102 92-93 2.9189682085309734 1102 94-95 3.215273168504037 1102 96-97 3.3401232941990315 1102 98-99 3.5042668635460537 1102 100-101 3.340203313047933 1103 1 0.2242836953196914 1103 2 0.17359253581295775 1103 3 0.19558597297512392 1103 4 0.0948116721329626 1103 5 0.02952425533914038 1103 6 0.054090080050876566 1103 7 0.16381690280393002 1103 8 0.12578166515385902 1103 9 0.26078197368953226 1103 10-11 0.30619414966286485 1103 12-13 0.3177811322185562 1103 14-15 0.39562134569980145 1103 16-17 0.41714879958561113 1103 18-19 0.37612375912498663 1103 20-21 0.34145680900109454 1103 22-23 0.44352609944563426 1103 24-25 0.40639348932501207 1103 26-27 0.4258624686000658 1103 28-29 0.5090930784392924 1103 30-31 0.427301420144218 1103 32-33 0.39214424994481334 1103 34-35 0.4586222983819539 1103 36-37 0.47939135037082536 1103 38-39 0.5076938457162257 1103 40-41 0.5875462760125743 1103 42-43 0.5978401207537516 1103 44-45 0.7017744969497457 1103 46-47 0.6337513623697575 1103 48-49 0.7038668544891422 1103 50-51 0.9302433787580995 1103 52-53 0.9326784141939584 1103 54-55 0.9787293527082497 1103 56-57 1.0761008858996277 1103 58-59 1.1759721769926443 1103 60-61 1.1934194543456158 1103 62-63 1.3763968675103087 1103 64-65 1.3439010905240778 1103 66-67 1.5531938894830972 1103 68-69 1.6442536300917538 1103 70-71 1.7362570907946377 1103 72-73 1.972087151435293 1103 74-75 2.0886469187502783 1103 76-77 1.9153287927285625 1103 78-79 1.9358221529023787 1103 80-81 2.201830579956301 1103 82-83 2.410307424167943 1103 84-85 2.526343255342038 1103 86-87 2.5872846108375747 1103 88-89 2.7463778113268162 1103 90-91 2.973003385864317 1103 92-93 3.148861631995164 1103 94-95 3.3752433162170163 1103 96-97 3.6114399362372325 1103 98-99 3.9089788216807726 1103 100-101 3.9177168075364506 1104 1 0.27453001406782107 1104 2 0.25928237205707205 1104 3 0.24281482363983997 1104 4 0.1977683426949568 1104 5 0.033542895402590034 1104 6 0.12296589244612477 1104 7 0.2047710941123313 1104 8 0.21423952207082664 1104 9 0.18258782073683477 1104 10-11 0.25151865960440745 1104 12-13 0.3073679712040587 1104 14-15 0.4626778831667977 1104 16-17 0.4722959143669385 1104 18-19 0.4339408067838164 1104 20-21 0.41110062346633214 1104 22-23 0.43938294707301395 1104 24-25 0.4582922914700944 1104 26-27 0.48029675569627983 1104 28-29 0.5591594990972624 1104 30-31 0.5338708502372143 1104 32-33 0.5445022890006683 1104 34-35 0.6283359581221646 1104 36-37 0.7079462719259553 1104 38-39 0.7488052734517368 1104 40-41 0.698707018232767 1104 42-43 0.6501171105407835 1104 44-45 0.7343314005548152 1104 46-47 0.8725767525647043 1104 48-49 0.9233837467295487 1104 50-51 1.0764489454096022 1104 52-53 1.1852796433354982 1104 54-55 1.2967156102698851 1104 56-57 1.223721326719712 1104 58-59 1.439335793523476 1104 60-61 1.4789640191824986 1104 62-63 1.651773702217156 1104 64-65 1.6125635619644711 1104 66-67 1.755506732050499 1104 68-69 1.873310617409679 1104 70-71 2.0560302796795966 1104 72-73 2.199713832668561 1104 74-75 2.0975716397496953 1104 76-77 2.157228093768463 1104 78-79 2.3507857420362797 1104 80-81 2.369038138804747 1104 82-83 2.5346890905442905 1104 84-85 2.7407162460220036 1104 86-87 2.8414028184691453 1104 88-89 3.1413739808292895 1104 90-91 3.310887936529202 1104 92-93 3.6790105225038197 1104 94-95 4.008505944597875 1104 96-97 4.384235413036929 1104 98-99 4.68722324462858 1104 100-101 4.1093558376849195 1105 1 0.17544685094002688 1105 2 0.22848464117556233 1105 3 0.23517992831218493 1105 4 0.15599194893081147 1105 5 0.0779145853243648 1105 6 -0.010860306325795932 1105 7 0.0929858355893387 1105 8 0.14209402312777542 1105 9 0.24161964619525378 1105 10-11 0.19361562967528556 1105 12-13 0.2251359678010303 1105 14-15 0.3594103427964228 1105 16-17 0.3495092628104075 1105 18-19 0.29645667729988645 1105 20-21 0.31386184972550524 1105 22-23 0.3005613917462 1105 24-25 0.2570034107351944 1105 26-27 0.35386150902543534 1105 28-29 0.39734761382607076 1105 30-31 0.4511546134409983 1105 32-33 0.42326778203387505 1105 34-35 0.40531948850425437 1105 36-37 0.42245111182451467 1105 38-39 0.42743462911690244 1105 40-41 0.4936088077450762 1105 42-43 0.3855909205362096 1105 44-45 0.5245150601740534 1105 46-47 0.5459233776243693 1105 48-49 0.6356926916933645 1105 50-51 0.6800862566275825 1105 52-53 0.7254819741501137 1105 54-55 0.8317634734733446 1105 56-57 0.8281403927702797 1105 58-59 1.1096134423166717 1105 60-61 1.1455549186795082 1105 62-63 1.1217207896730343 1105 64-65 1.085447303736963 1105 66-67 1.1984057517315563 1105 68-69 1.5359208992310798 1105 70-71 1.4887973345988925 1105 72-73 1.6938429594086344 1105 74-75 1.7384190197750868 1105 76-77 1.802844340630891 1105 78-79 1.7271978526606766 1105 80-81 1.9653145341924798 1105 82-83 2.0292314189560052 1105 84-85 2.3132733230544886 1105 86-87 2.506193571194327 1105 88-89 2.6309700660478725 1105 90-91 2.8723445820135325 1105 92-93 3.165699989676611 1105 94-95 3.488101575213438 1105 96-97 3.6575679129313983 1105 98-99 3.719811786684229 1105 100-101 3.4388870120868695 1106 1 0.09607203717639123 1106 2 0.0704760690512316 1106 3 0.08983348393879709 1106 4 0.04241052347476426 1106 5 -0.04153732154149736 1106 6 0.04709411623839088 1106 7 0.0631992984459302 1106 8 0.0905891166018975 1106 9 0.15345447603259288 1106 10-11 0.16359792603676482 1106 12-13 0.11718704966411764 1106 14-15 0.03228505036157259 1106 16-17 0.07704448919991336 1106 18-19 0.010687722425295476 1106 20-21 -0.09388365745592608 1106 22-23 -0.04930370115997107 1106 24-25 0.08914230280096547 1106 26-27 -0.14959713852706358 1106 28-29 0.055059666537871976 1106 30-31 0.1835860570636001 1106 32-33 0.3052138635429671 1106 34-35 0.16364692840485873 1106 36-37 0.23163129059586396 1106 38-39 0.11158495171913074 1106 40-41 0.17591825925515536 1106 42-43 0.28038886720978695 1106 44-45 0.3120860828460721 1106 46-47 0.4146662247864512 1106 48-49 0.49261745570319704 1106 50-51 0.4693473135243025 1106 52-53 0.4626688150289908 1106 54-55 0.6737868970481671 1106 56-57 0.7563498239742756 1106 58-59 0.9132441664347724 1106 60-61 0.799721089364219 1106 62-63 0.778313479171203 1106 64-65 0.7805288742920524 1106 66-67 1.145791693385405 1106 68-69 1.0783885281416588 1106 70-71 1.0511482968816992 1106 72-73 1.1810431237245425 1106 74-75 1.4601352107269747 1106 76-77 1.4046454164643905 1106 78-79 1.5621619941372948 1106 80-81 1.3078376352817713 1106 82-83 1.8456791922498468 1106 84-85 1.7547593161348587 1106 86-87 2.021982330336929 1106 88-89 2.128549479567681 1106 90-91 2.1584503183658583 1106 92-93 2.128466040236429 1106 94-95 2.3923600374437335 1106 96-97 2.6833604337568033 1106 98-99 2.771350439433835 1106 100-101 2.8282351867565474 1107 1 0.252495516644359 1107 2 0.2162513297412758 1107 3 0.1761675010523973 1107 4 0.1672980645899571 1107 5 0.08388635472361017 1107 6 0.18852358185569784 1107 7 0.2009372580433464 1107 8 0.21969616989838414 1107 9 0.24637732191207817 1107 10-11 0.2957920099857816 1107 12-13 0.28839101524938116 1107 14-15 0.5055704529100282 1107 16-17 0.4319655803521556 1107 18-19 0.34638659141833017 1107 20-21 0.30749384691056747 1107 22-23 0.4332241722909558 1107 24-25 0.4535277106238098 1107 26-27 0.38372150591305854 1107 28-29 0.4344113929365321 1107 30-31 0.4078375331839439 1107 32-33 0.4979874385076428 1107 34-35 0.5548872059927987 1107 36-37 0.46513412279218613 1107 38-39 0.5789652112455954 1107 40-41 0.5970422110173672 1107 42-43 0.6115302093360313 1107 44-45 0.7326972466695736 1107 46-47 0.6918377942729563 1107 48-49 0.837112079322516 1107 50-51 0.8580123086440707 1107 52-53 1.0709487380720475 1107 54-55 1.0421379279456957 1107 56-57 1.123393411043594 1107 58-59 1.1838095149008865 1107 60-61 1.263504838608739 1107 62-63 1.3528883472500155 1107 64-65 1.5388928000339277 1107 66-67 1.7445699631245333 1107 68-69 1.9090254948870964 1107 70-71 1.9532963537415142 1107 72-73 2.0168700715483503 1107 74-75 1.9078549443759414 1107 76-77 1.846543376485272 1107 78-79 1.926879083596436 1107 80-81 2.0657672272764955 1107 82-83 2.418803130400722 1107 84-85 2.394939186074822 1107 86-87 2.4524808018035174 1107 88-89 2.6494769933770357 1107 90-91 2.894580798840124 1107 92-93 3.255501430260569 1107 94-95 3.358671618549014 1107 96-97 3.6395242960169547 1107 98-99 3.936166059795706 1107 100-101 3.699413309720164 1108 1 0.16583287817507397 1108 2 0.17289277221957633 1108 3 0.15031472207575547 1108 4 0.12523370934130895 1108 5 0.04924673452507733 1108 6 0.08577596760355277 1108 7 0.12791064316778744 1108 8 0.15617533356788016 1108 9 0.21329795683271158 1108 10-11 0.1881182939736945 1108 12-13 0.24470435146000113 1108 14-15 0.34331936193990487 1108 16-17 0.22976839049937325 1108 18-19 0.2804541116792052 1108 20-21 0.33408071690529795 1108 22-23 0.30778696901084857 1108 24-25 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0.037763776632523616 2316 52-53 0.1455386442190445 2316 54-55 0.44882839251523876 2316 56-57 0.43047403000677065 2316 58-59 0.5645066766574871 2316 60-61 0.7768262509255734 2316 62-63 0.8446329991942818 2316 64-65 0.9721776932052109 2316 66-67 1.1210050378618348 2316 68-69 1.2871066966620646 2316 70-71 1.486288620085034 2316 72-73 1.5619804689283114 2316 74-75 1.8684181359133127 2316 76-77 2.019122743771142 2316 78-79 1.9742697578428938 2316 80-81 2.0701425080543814 2316 82-83 2.1127030827107696 2316 84-85 2.3720925079402093 2316 86-87 2.4603538359169796 2316 88-89 2.7793762090082765 2316 90-91 2.9846304422834606 2316 92-93 2.980590124447371 2316 94-95 3.526883280727649 2316 96-97 4.139488550619504 2316 98-99 4.637187510270383 2316 100-101 4.841545983102261 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 29.0 5 37.0 6 90.0 7 218.0 8 581.0 9 1162.0 10 2160.0 11 3453.0 12 5362.0 13 8376.0 14 10997.0 15 15441.0 16 19975.0 17 27509.0 18 28583.0 19 23026.0 20 21674.0 21 22312.0 22 24455.0 23 26909.0 24 30131.0 25 33946.0 26 38303.0 27 44776.0 28 53436.0 29 63961.0 30 76395.0 31 92200.0 32 112105.0 33 140369.0 34 183342.0 35 250590.0 36 345656.0 37 436442.0 38 246438.0 39 11299.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.042317512078704 19.219251978142587 15.250600812408296 22.487829697370415 2 15.850268778136964 20.168749945344906 40.23222897423736 23.748752302280774 3 19.675651233264798 24.130210844602555 29.951983491514667 26.242154430617976 4 13.226270503663024 17.35521223464164 37.147901178537744 32.27061608315759 5 13.744228945421197 37.38884662282576 34.24018008816817 14.62674434358488 6 31.803045115474234 36.63444291407758 17.169952884315922 14.392559086132268 7 28.28727243657536 32.94671214465465 20.366707914402344 18.399307504367652 8 26.2245991976667 35.04526916887513 19.442780145269378 19.287351488188794 9 25.977621299153782 17.10537491860095 20.51872219733294 36.398281584912326 10-11 24.948912208941152 26.821902214553734 27.024317288907913 21.2048682875972 12-13 25.837286010022698 24.202497514310412 27.609661796325007 22.35055467934188 14-15 22.498238782753226 25.69411944966749 26.336172654250156 25.471469113329125 16-17 21.693433635300963 28.8174339725554 27.228486758079274 22.260645634064364 18-19 21.561629280007725 26.962633048929362 30.05513067982153 21.420606991241378 20-21 22.086809273912166 26.062725981243663 30.587034942883268 21.263429801960907 22-23 21.836950778622676 26.14542950301469 30.35866481856287 21.65895489979977 24-25 21.70918655191093 26.291074925293717 30.423684671814883 21.57605385098048 26-27 21.30311079206548 26.662584677676236 30.222971535309483 21.8113329949488 28-29 21.434430256629756 26.960322231974377 30.1016592914827 21.503588219913166 30-31 21.55494664719196 26.526450735047973 30.06212558519878 21.856477032561283 32-33 21.265761437086656 26.54160636336535 30.05991885909762 22.132713340450373 34-35 21.96757975817965 26.778127860849928 29.799872454370586 21.454419926599844 36-37 22.078774817506698 26.273747445593433 29.7836311461895 21.86384659071037 38-39 21.53337290447982 26.75105966630739 29.656415990879342 22.059151438333448 40-41 22.13887899929836 26.52375687847047 29.683533103062025 21.65383101916915 42-43 21.796684145197414 26.497566427230367 29.59794105045234 22.107808377119877 44-45 21.8460839589736 26.822469170449097 29.642794835100368 21.688652035476935 46-47 22.14783194319969 27.08738116725426 28.84597569061694 21.918811198929113 48-49 21.885789016999286 27.17370848232204 28.790197805931346 22.150304694747327 50-51 22.09942508539614 27.37693546533608 28.703302761155626 21.820336688112157 52-53 22.46245234720712 27.11883178418385 28.557679656650077 21.861036211958957 54-55 22.015049039283056 27.333592033802045 28.72986674705256 21.921492179862334 56-57 22.063254438596612 27.48254445882982 28.451943993209937 22.002257109363633 58-59 22.029705031502054 27.20156319480645 28.41422164190415 22.354510131787347 60-61 21.771663804862126 27.399814635007502 28.6764265026972 22.15209505743317 62-63 21.865241482453612 27.369066196786672 28.79382016764457 21.97187215311515 64-65 21.65104332344609 27.344542391624767 28.654796423271716 22.349617861657425 66-67 21.55017354056053 27.457089514952465 28.500289789419686 22.492447155067318 68-69 22.096962532916496 27.108680169619998 28.64653541220329 22.147821885260218 70-71 21.880422484045468 27.311967640228623 28.208502350892424 22.59910752483348 72-73 21.927436494597988 27.284271911520978 28.243470527360426 22.54482106652061 74-75 21.942955713921695 27.72212155480092 27.994194228536884 22.340728502740504 76-77 21.90469191605447 27.325098220129675 28.3937781982123 22.37643166560355 78-79 22.04167547969229 27.52022693471588 28.258897269821816 22.179200315770014 80-81 22.140883129644415 27.653901282089983 27.958804909352274 22.246410678913332 82-83 22.0825454350895 27.692790364140667 28.081557383107057 22.143106817662773 84-85 22.08251444846022 27.712771272963476 28.03265685115171 22.172057427424594 86-87 22.23184512984992 27.670608427071674 27.784279023423846 22.31326741965456 88-89 22.065803739609652 27.938630530763426 27.46541269108999 22.53015303853693 90-91 22.04982004293654 27.750528379253893 27.801305250815677 22.39834632699389 92-93 22.119056620428122 27.448529154599065 27.877258563346363 22.55515566162645 94-95 21.88514820979308 27.39588570405507 27.979544061147145 22.73942202500471 96-97 22.16161121151263 27.143134024816533 27.90818177862725 22.787072985043586 98-99 21.87144529734177 27.39943990793372 27.57597799602623 23.15313679869828 100-101 22.3221378762468 26.822360214310574 27.83239540534644 23.023106504096184 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 896.0 1 626.5 2 488.5 3 688.5 4 578.5 5 481.5 6 969.5 7 2301.0 8 3709.5 9 4274.0 10 3567.5 11 2574.5 12 2384.5 13 2524.5 14 2768.5 15 2957.0 16 3190.5 17 3507.0 18 3811.5 19 4211.0 20 4791.5 21 5634.5 22 6852.5 23 8403.0 24 10411.0 25 13256.5 26 17067.0 27 21780.5 28 25435.5 29 28382.0 30 32225.0 31 37555.5 32 44665.0 33 48307.5 34 49749.5 35 52897.0 36 57210.5 37 61748.0 38 65002.0 39 67433.5 40 70689.0 41 75244.0 42 77164.5 43 78316.5 44 81190.5 45 84432.0 46 85660.0 47 86201.0 48 86466.5 49 84766.0 50 83106.5 51 81176.0 52 79361.5 53 78770.0 54 78629.0 55 74066.5 56 67498.5 57 61383.5 58 56443.5 59 50125.5 60 39948.5 61 32858.0 62 27683.5 63 20411.5 64 14137.0 65 10603.0 66 8071.5 67 6133.5 68 4511.0 69 3341.5 70 2545.5 71 1745.0 72 1207.0 73 921.5 74 684.0 75 510.0 76 361.5 77 215.5 78 133.0 79 81.5 80 41.5 81 19.0 82 8.5 83 6.0 84 2.5 85 1.0 86 2.0 87 1.0 88 1.5 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.013531810997656707 3 0.0030810892733126043 4 0.0017903626858438103 5 0.12170302629584785 6 0.0 7 0.0 8 1.249090245937542E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.0818170765625703E-5 18-19 0.0 20-21 0.0 22-23 2.9145439071875983E-4 24-25 3.7472707378126267E-4 26-27 0.005870724155906448 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010596448919703483 36-37 6.661814645000225E-4 38-39 0.006932450864953359 40-41 0.009701267576781577 42-43 0.005620906106718939 44-45 0.0031227256148438553 46-47 2.0818170765625703E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.4572719535937991E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.6654536612500562E-4 68-69 0.0011033630505781623 70-71 2.0818170765625703E-5 72-73 1.4572719535937991E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.2899987842188275E-4 82-83 0.0021859079303906985 84-85 0.004496724885375151 86-87 0.0029769984194844756 88-89 0.0 90-91 0.00266472585800009 92-93 0.0 94-95 2.0818170765625703E-5 96-97 1.0409085382812852E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2401748.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.223244229975315 #Duplication Level Percentage of deduplicated Percentage of total 1 77.5811235090252 45.170247017018546 2 13.417045875887478 15.623678777531596 3 4.09254893700689 7.14844428847434 4 1.7783320489344459 4.141610448284107 5 0.878846492573287 2.558464698887584 6 0.5097939567769747 1.780911483143477 7 0.34319784820090377 1.398746449450225 8 0.21966603954616887 1.0231735575622405 9 0.1737013386711653 0.9102109918072423 >10 0.9019086835569855 9.60017212271738 >50 0.06496584308813035 2.599475534806819 >100 0.03350708550102838 3.6127735138904535 >500 0.0029314132064586383 1.1925700332815545 >1k 0.002216434375613488 2.1007765543675454 >5k 1.4299576616861215E-4 0.6640560308188067 >10k+ 7.149788308430608E-5 0.4746884979580525 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11403 0.47477920248085975 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9894 0.4119499631102014 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6058 0.25223295699632103 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2749 0.11445830286941011 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2727 0.11354230335572257 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2623 0.10921212383647244 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2582 0.10750503383369113 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2536 0.10558976212325355 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 4.1636341531251406E-5 5 0.0 4.1636341531251406E-5 0.0 0.0 4.1636341531251406E-5 6 0.0 4.1636341531251406E-5 0.0 0.0 4.1636341531251406E-5 7 0.0 4.1636341531251406E-5 0.0 4.1636341531251406E-5 4.1636341531251406E-5 8 0.0 4.1636341531251406E-5 0.0 4.1636341531251406E-5 4.1636341531251406E-5 9 0.0 4.1636341531251406E-5 0.0 8.327268306250281E-5 4.1636341531251406E-5 10-11 4.1636341531251406E-5 4.1636341531251406E-5 0.0 1.6654536612500562E-4 4.1636341531251406E-5 12-13 8.327268306250281E-5 4.1636341531251406E-5 0.0 1.6654536612500562E-4 1.4572719535937991E-4 14-15 8.327268306250281E-5 4.1636341531251406E-5 0.0 1.873635368906313E-4 1.6654536612500562E-4 16-17 8.327268306250281E-5 4.1636341531251406E-5 0.0 2.498180491875084E-4 2.498180491875084E-4 18-19 8.327268306250281E-5 4.1636341531251406E-5 0.0 2.9145439071875983E-4 2.498180491875084E-4 20-21 8.327268306250281E-5 4.1636341531251406E-5 0.0 2.9145439071875983E-4 2.498180491875084E-4 22-23 8.327268306250281E-5 4.1636341531251406E-5 0.0 3.9554524454688835E-4 2.498180491875084E-4 24-25 8.327268306250281E-5 8.327268306250281E-5 0.0 5.829087814375198E-4 2.498180491875084E-4 26-27 1.249090245937542E-4 1.249090245937542E-4 0.0 0.001457271953593799 2.498180491875084E-4 28-29 1.249090245937542E-4 1.249090245937542E-4 0.0 0.004434270373078275 2.498180491875084E-4 30-31 1.249090245937542E-4 1.249090245937542E-4 0.0 0.011241812213437879 2.498180491875084E-4 32-33 1.249090245937542E-4 1.249090245937542E-4 0.0 0.02606434979856338 2.498180491875084E-4 34-35 1.6654536612500562E-4 1.249090245937542E-4 0.0 0.048131610810126624 2.498180491875084E-4 36-37 2.498180491875084E-4 1.6654536612500562E-4 0.0 0.08608313611586227 2.498180491875084E-4 38-39 2.9145439071875983E-4 1.6654536612500562E-4 0.0 0.14239628803687981 2.498180491875084E-4 40-41 2.9145439071875983E-4 1.873635368906313E-4 0.0 0.2033935283801631 2.498180491875084E-4 42-43 2.9145439071875983E-4 2.0818170765625702E-4 0.0 0.26468222311416517 2.498180491875084E-4 44-45 3.3309073225001125E-4 2.0818170765625702E-4 0.0 0.34046036470104274 2.498180491875084E-4 46-47 3.3309073225001125E-4 2.0818170765625702E-4 0.0 0.4285836815519364 2.7063621995313415E-4 48-49 3.3309073225001125E-4 2.0818170765625702E-4 0.0 0.5230773586571114 2.9145439071875983E-4 50-51 3.3309073225001125E-4 2.7063621995313415E-4 0.0 0.6210684884509117 2.9145439071875983E-4 52-53 3.3309073225001125E-4 3.3309073225001125E-4 0.0 0.7152290748238366 3.3309073225001125E-4 54-55 3.3309073225001125E-4 3.3309073225001125E-4 0.0 0.8158641123048713 3.7472707378126267E-4 56-57 3.3309073225001125E-4 3.3309073225001125E-4 0.0 0.9335492316429534 3.7472707378126267E-4 58-59 3.3309073225001125E-4 3.3309073225001125E-4 0.0 1.0608731640455202 3.7472707378126267E-4 60-61 3.3309073225001125E-4 3.3309073225001125E-4 0.0 1.197711000487978 3.7472707378126267E-4 62-63 3.3309073225001125E-4 3.3309073225001125E-4 0.0 1.3311346569248732 3.7472707378126267E-4 64-65 3.3309073225001125E-4 3.3309073225001125E-4 0.0 1.4698044923947058 3.7472707378126267E-4 66-67 3.7472707378126267E-4 3.3309073225001125E-4 0.0 1.6257742277707736 3.9554524454688835E-4 68-69 3.7472707378126267E-4 3.3309073225001125E-4 0.0 1.7960668646335918 4.1636341531251403E-4 70-71 3.9554524454688835E-4 3.3309073225001125E-4 0.0 1.973937315655098 4.1636341531251403E-4 72-73 4.5799975684376545E-4 3.3309073225001125E-4 0.0 2.1607387619350575 4.371815860781397E-4 74-75 5.204542691406426E-4 3.3309073225001125E-4 0.0 2.348352116874876 4.5799975684376545E-4 76-77 5.412724399062683E-4 3.3309073225001125E-4 0.0 2.546208011831383 4.5799975684376545E-4 78-79 5.412724399062683E-4 3.3309073225001125E-4 0.0 2.769399620609656 4.5799975684376545E-4 80-81 5.829087814375197E-4 3.3309073225001125E-4 0.0 3.0055817679456798 4.7881792760939113E-4 82-83 5.829087814375197E-4 3.3309073225001125E-4 0.0 3.2481967300482815 5.412724399062683E-4 84-85 6.037269522031454E-4 3.3309073225001125E-4 0.0 3.4875224211699143 5.62090610671894E-4 86-87 6.245451229687711E-4 3.3309073225001125E-4 0.0 3.7368200160882825 5.829087814375197E-4 88-89 6.245451229687711E-4 3.3309073225001125E-4 0.0 4.0030636020098695 5.829087814375197E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5035 0.0 61.385788 1 GTATCAA 9315 0.0 43.236897 1 TGGTATC 1785 0.0 41.023857 2 GTGGTAT 2165 0.0 35.14116 1 ATCAACG 11765 0.0 33.945873 3 TATCAAC 11905 0.0 33.6707 2 TCAACGC 11990 0.0 33.308167 4 CAACGCA 12155 0.0 32.856018 5 AACGCAG 12640 0.0 31.675817 6 ACGCAGA 14375 0.0 27.720526 7 CGCAGAG 14470 0.0 27.440062 8 GCAGAGT 16005 0.0 24.363228 9 GAGTACT 11155 0.0 22.88528 12-13 TAAGGTG 1530 0.0 22.684124 5 GTAAGGT 1490 0.0 22.335844 4 CAGAGTA 15480 0.0 21.983286 10-11 GTACTTT 11590 0.0 21.964872 14-15 GTACATG 8065 0.0 21.107296 1 AGAGTAC 15075 0.0 20.54176 10-11 TACATGG 8080 0.0 20.185371 2 >>END_MODULE