FastQCFastQC Report
Thu 26 May 2016
SRR938242_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938242_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2400563
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118880.49521716364036267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99360.4139029052768038No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60470.251899241969488No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27540.11472308787563584No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26960.11230698798573502No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25020.10422555042296329No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24780.10322578495128018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48850.059.043511
GTATCAA89850.043.740281
TGGTATC19050.038.17042
TATCAAC114750.034.2103542
TCAACGC114650.033.9914744
ATCAACG114950.033.944113
CAACGCA117200.033.292455
GTGGTAT21900.032.925521
AACGCAG121750.032.12036
ACGCAGA137150.028.2300057
CGCAGAG138150.028.0600478
GCAGAGT150550.024.8958689
GAGTACT101300.023.11728712-13
CAGAGTA143150.022.48108910-11
GTACTTT106900.021.84167514-15
GTACATG81550.021.548831
TACATGG80300.021.3659342
TAAGGTG15150.021.331755
TAGCCCT18800.020.7293764
AGAGTAC139650.020.69755410-11
Produced by FastQC (version 0.11.5)