FastQCFastQC Report
Thu 26 May 2016
SRR938242_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938242_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2400563
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116350.48467796929303664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102400.42656660125145646No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64030.26672909646612064No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28080.11697256018692283No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27790.1157645102419724No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC26710.11126556561939845No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25410.10585016931444831No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24340.10139288158652782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48000.061.1585961
GTATCAA85750.042.377721
TGGTATC17900.035.2953832
GTGGTAT20000.033.2512741
ATCAACG110400.032.311943
TATCAAC111400.032.1518142
TCAACGC112600.031.847374
CAACGCA114750.031.2092785
AACGCAG119500.029.968746
ACGCAGA135550.026.2800877
CGCAGAG136850.026.0304418
TAAGGTG17550.024.8986475
GCAGAGT151400.023.2773769
GTAAGGT17050.022.5644974
GGTAAGG16700.022.4699843
GAGTACT105150.021.97503512-13
GTACATG82000.021.897181
TACATGG81800.021.718892
CAGAGTA146900.021.43619510-11
GTGTACG1805.170903E-621.1119211
Produced by FastQC (version 0.11.5)