##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938242_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2400563 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.502537529737815 33.0 31.0 34.0 31.0 34.0 2 32.73255607122162 34.0 31.0 34.0 31.0 34.0 3 32.69304284036703 34.0 31.0 34.0 31.0 34.0 4 36.213579064577765 37.0 35.0 37.0 35.0 37.0 5 36.182151853544354 37.0 35.0 37.0 35.0 37.0 6 36.24395443902118 37.0 37.0 37.0 35.0 37.0 7 36.207489659717325 37.0 37.0 37.0 35.0 37.0 8 36.201102824629054 37.0 37.0 37.0 35.0 37.0 9 37.911884837015315 39.0 38.0 39.0 35.0 39.0 10-11 37.87456005112134 39.0 38.0 39.0 35.0 39.0 12-13 37.818896858778544 39.0 38.0 39.0 35.0 39.0 14-15 39.09350285745469 40.0 38.5 41.0 36.0 41.0 16-17 39.03178150292244 40.0 38.0 41.0 36.0 41.0 18-19 39.03678137170323 40.0 38.5 41.0 36.0 41.0 20-21 39.04432814302312 40.0 38.5 41.0 35.5 41.0 22-23 39.071904798999235 40.0 39.0 41.0 35.5 41.0 24-25 39.02306688056093 40.0 38.5 41.0 35.0 41.0 26-27 38.855556175780436 40.0 38.0 41.0 35.0 41.0 28-29 38.775382899761425 40.0 38.0 41.0 35.0 41.0 30-31 38.542244881721494 40.0 38.0 41.0 34.5 41.0 32-33 38.326900189663846 40.0 38.0 41.0 34.0 41.0 34-35 38.11808563241206 40.0 38.0 41.0 34.0 41.0 36-37 38.027030742371686 40.0 38.0 41.0 33.5 41.0 38-39 37.966250417089654 40.0 38.0 41.0 33.0 41.0 40-41 37.834124536619115 40.0 37.5 41.0 33.0 41.0 42-43 37.635594858372805 40.0 37.0 41.0 33.0 41.0 44-45 37.37175008529249 40.0 37.0 41.0 32.5 41.0 46-47 36.99598739962251 40.0 37.0 41.0 31.5 41.0 48-49 36.6100656387689 40.0 36.0 41.0 30.5 41.0 50-51 36.30886671168389 39.0 36.0 41.0 30.0 41.0 52-53 35.844540634842744 39.0 35.0 40.5 28.5 41.0 54-55 35.52880990834234 39.0 35.0 40.0 28.0 41.0 56-57 35.263100780941805 38.0 35.0 40.0 27.0 41.0 58-59 34.89028282115487 38.0 34.0 40.0 26.5 41.0 60-61 34.48563607787007 38.0 34.0 40.0 26.0 41.0 62-63 34.06271612117658 37.0 33.5 40.0 25.0 41.0 64-65 33.63364989796143 36.5 33.0 39.0 23.5 41.0 66-67 33.12425085282078 36.0 32.5 39.0 22.5 40.0 68-69 32.601044005093804 35.0 32.0 39.0 21.5 40.0 70-71 32.017628989532874 35.0 31.0 38.0 19.0 40.0 72-73 31.54705937732107 35.0 31.0 37.0 18.0 39.5 74-75 30.990359761439294 34.5 30.5 37.0 14.5 39.0 76-77 29.789325670686416 33.5 29.0 35.5 13.0 37.5 78-79 30.478673752782157 34.0 30.0 36.0 12.0 38.0 80-81 30.283755102448886 34.0 30.0 35.5 7.5 37.0 82-83 29.813578314753663 34.0 30.0 35.0 4.5 37.0 84-85 29.297587899171987 34.0 29.0 35.0 2.0 36.5 86-87 28.757349421781473 34.0 29.0 35.0 2.0 36.0 88-89 28.13829547485319 33.5 27.5 35.0 2.0 35.5 90-91 27.60756268425365 33.0 26.5 35.0 2.0 35.0 92-93 26.980469164941724 33.0 25.5 35.0 2.0 35.0 94-95 26.245374939128865 32.0 24.5 34.5 2.0 35.0 96-97 25.43203511009709 32.0 21.5 34.0 2.0 35.0 98-99 24.42894146081565 32.0 10.0 34.0 2.0 35.0 100-101 20.944641111272645 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.01447284519321812 1101 2 0.02275453390814164 1101 3 0.16893919486704334 1101 4 0.047459071378035844 1101 5 0.010950059276972013 1101 6 0.05389808001319096 1101 7 0.038907316779933865 1101 8 0.10968707369578823 1101 9 0.0683804827773784 1101 10-11 0.15566633349281744 1101 12-13 0.201468132909973 1101 14-15 0.22885469577084905 1101 16-17 0.2818062669466599 1101 18-19 0.26838497716511966 1101 20-21 0.22404552340356787 1101 22-23 0.1127510138664789 1101 24-25 0.07856338396454277 1101 26-27 0.27065167197097395 1101 28-29 0.16910362974595472 1101 30-31 0.15823985640503935 1101 32-33 0.14401951281352154 1101 34-35 0.13091161160806308 1101 36-37 0.17736076737443085 1101 38-39 0.241726694597709 1101 40-41 0.28053052319315697 1101 42-43 0.3838761232958703 1101 44-45 0.3219283247494502 1101 46-47 0.457447225436006 1101 48-49 0.45280886977757717 1101 50-51 0.402807276843987 1101 52-53 0.37286146461764247 1101 54-55 0.3640074930729895 1101 56-57 0.3863171363979845 1101 58-59 0.5089324045835397 1101 60-61 0.49230174986961117 1101 62-63 0.5092500664941184 1101 64-65 0.5447562343294479 1101 66-67 0.6345181023734625 1101 68-69 0.6173695323519297 1101 70-71 0.6609839220531413 1101 72-73 0.7078165861401935 1101 74-75 0.6754871154151374 1101 76-77 0.7645146699219616 1101 78-79 0.8088944904648194 1101 80-81 0.9944521465615672 1101 82-83 1.023894725718339 1101 84-85 1.0479070083106095 1101 86-87 1.1938644217788728 1101 88-89 1.3586434004112178 1101 90-91 1.2335655269925674 1101 92-93 1.730564414285734 1101 94-95 1.7784809041513654 1101 96-97 1.9654551753715133 1101 98-99 2.0450416494090895 1101 100-101 2.408445304391009 1102 1 -0.025296974238763426 1102 2 0.018298607625098384 1102 3 0.1282010791290773 1102 4 0.0238595637189718 1102 5 -0.036650853841713626 1102 6 0.006479515388278401 1102 7 0.07592771658760711 1102 8 0.013595653450458656 1102 9 0.01986245134978759 1102 10-11 0.07629764634176439 1102 12-13 -0.02086306356279266 1102 14-15 0.05404861307236786 1102 16-17 0.07583858993744741 1102 18-19 0.12021779456116377 1102 20-21 0.08868584960094239 1102 22-23 0.1256200222777366 1102 24-25 0.01847826506386241 1102 26-27 0.1244565625231715 1102 28-29 0.044727012155696855 1102 30-31 0.1381492670495419 1102 32-33 0.0474052383669914 1102 34-35 -0.10300957341630834 1102 36-37 0.1213713934748668 1102 38-39 0.19014507945453119 1102 40-41 0.24337658751917246 1102 42-43 0.24615452041820873 1102 44-45 0.19222640002669067 1102 46-47 0.2288884408403291 1102 48-49 0.1980342636976289 1102 50-51 0.2678673486308085 1102 52-53 0.15721931837587988 1102 54-55 0.2897448862980525 1102 56-57 0.3890230117180593 1102 58-59 0.35517123036245124 1102 60-61 0.394366093944015 1102 62-63 0.3142877016749921 1102 64-65 0.37531792789968677 1102 66-67 0.45536350267570924 1102 68-69 0.5214663740244063 1102 70-71 0.7414000378754011 1102 72-73 0.5893093106933343 1102 74-75 0.6337178060428563 1102 76-77 0.6595756921572935 1102 78-79 0.6952171922501975 1102 80-81 0.8322729444394419 1102 82-83 0.9487005933832222 1102 84-85 1.0513973262076846 1102 86-87 1.2035460946370726 1102 88-89 1.231692185514376 1102 90-91 1.3009915704861221 1102 92-93 1.4749354797084173 1102 94-95 1.524389352305473 1102 96-97 1.609110415439325 1102 98-99 1.8833711092704881 1102 100-101 2.372170641112124 1103 1 0.20265319163684836 1103 2 0.24549083718044074 1103 3 0.34567924663879523 1103 4 0.17682333873992917 1103 5 0.0662435925201379 1103 6 0.09198544857316193 1103 7 0.1471639200335133 1103 8 0.2108248717069401 1103 9 0.17771897825564764 1103 10-11 0.2586493770930005 1103 12-13 0.22813876474161532 1103 14-15 0.30769604011376117 1103 16-17 0.3002887402166152 1103 18-19 0.32950783488046653 1103 20-21 0.32116111919282986 1103 22-23 0.2962297773936129 1103 24-25 0.30915854121128206 1103 26-27 0.42700295687946976 1103 28-29 0.41011659092119856 1103 30-31 0.38812637551116325 1103 32-33 0.41104424333470746 1103 34-35 0.2895796809433975 1103 36-37 0.36307056948010796 1103 38-39 0.44197187452846975 1103 40-41 0.5788120852013421 1103 42-43 0.61055800214573 1103 44-45 0.31699597531066814 1103 46-47 0.21771133278119947 1103 48-49 0.22069713825099768 1103 50-51 0.3760833230003371 1103 52-53 0.27803934862289736 1103 54-55 0.21836190029559788 1103 56-57 0.3193085924638197 1103 58-59 0.40205976718563363 1103 60-61 0.4092867231946684 1103 62-63 0.5233981314623719 1103 64-65 0.4223945023595732 1103 66-67 0.41136269568609407 1103 68-69 0.4564638383463162 1103 70-71 0.581437992549148 1103 72-73 0.3932029098167149 1103 74-75 0.39295403866918477 1103 76-77 0.1850532197101522 1103 78-79 0.5707081025485756 1103 80-81 0.5916652671708889 1103 82-83 0.6616073709891594 1103 84-85 0.7622219800423444 1103 86-87 0.8174443274103069 1103 88-89 0.8311673655525524 1103 90-91 1.1117216898139262 1103 92-93 1.517154016924021 1103 94-95 1.4037752201262066 1103 96-97 1.5543529751862408 1103 98-99 2.066971435084998 1103 100-101 2.2161024213090847 1104 1 0.15938630055191538 1104 2 0.12962542492925877 1104 3 0.1630533331313515 1104 4 0.0803350135201697 1104 5 -0.00576315697470875 1104 6 0.10601025397440367 1104 7 0.0880888930499637 1104 8 0.08565852180453248 1104 9 0.11386053993284406 1104 10-11 0.0898445612074994 1104 12-13 0.06958105704155315 1104 14-15 0.11200088130528485 1104 16-17 0.21691202785177666 1104 18-19 0.25638097886670863 1104 20-21 0.2607898740745469 1104 22-23 0.25057083711835304 1104 24-25 0.196246261972 1104 26-27 0.299693953820082 1104 28-29 0.17869756871396447 1104 30-31 0.19638216286176657 1104 32-33 0.32550289248762 1104 34-35 0.2594674573086664 1104 36-37 0.1993854717060941 1104 38-39 0.3281533876639031 1104 40-41 0.35772994459098584 1104 42-43 0.43703172315515815 1104 44-45 0.5323366253297124 1104 46-47 0.49697672061950726 1104 48-49 0.561832781066407 1104 50-51 0.6562982020730246 1104 52-53 0.6542775403848538 1104 54-55 0.765604013002914 1104 56-57 0.8174083504191216 1104 58-59 0.7216225386099282 1104 60-61 0.7775024468702725 1104 62-63 0.7137691773386408 1104 64-65 0.8366366189601209 1104 66-67 0.9154199042505695 1104 68-69 1.0244694876588483 1104 70-71 1.0560664691790933 1104 72-73 0.9292570111218872 1104 74-75 0.8980325342958189 1104 76-77 0.8151512673903696 1104 78-79 1.0527040677833632 1104 80-81 1.3346591050964491 1104 82-83 1.4157981518552383 1104 84-85 1.5088267931338741 1104 86-87 1.5212760031798922 1104 88-89 1.5589038457798452 1104 90-91 1.504256045892376 1104 92-93 1.7799748395003867 1104 94-95 1.933894199668174 1104 96-97 2.1215860464285434 1104 98-99 2.4678448708348313 1104 100-101 2.6981906791071744 1105 1 0.09970160126305672 1105 2 0.10683439823678498 1105 3 0.17629175774481354 1105 4 0.12882114785211485 1105 5 0.10673362467419167 1105 6 0.08293461178691786 1105 7 0.041122667710226324 1105 8 0.012044090035217891 1105 9 0.04911708552726424 1105 10-11 0.07191266108436878 1105 12-13 0.05768357602701002 1105 14-15 0.0845986858927148 1105 16-17 0.1247082791233396 1105 18-19 0.16842623169293347 1105 20-21 0.13992970241647384 1105 22-23 0.09007622864535136 1105 24-25 0.147891455385178 1105 26-27 0.15215583462359206 1105 28-29 0.12290869531235415 1105 30-31 0.1441367177903956 1105 32-33 0.1415996105735573 1105 34-35 0.17340109763249956 1105 36-37 0.20353625843623746 1105 38-39 0.1243456092918791 1105 40-41 0.05202203789990989 1105 42-43 0.09884004136288382 1105 44-45 0.23270541507927334 1105 46-47 0.19278785835352608 1105 48-49 0.2474284531125832 1105 50-51 0.28528867644543965 1105 52-53 0.38064369043808455 1105 54-55 0.3699941810764926 1105 56-57 0.528924782122381 1105 58-59 0.5280054993061896 1105 60-61 0.4936607394263035 1105 62-63 0.5105052981401101 1105 64-65 0.5380233500335763 1105 66-67 0.5491059290937343 1105 68-69 0.6032366529905389 1105 70-71 0.6495252235350009 1105 72-73 0.6493502944555694 1105 74-75 0.5654743718398585 1105 76-77 0.6457044987013241 1105 78-79 0.798568713576266 1105 80-81 0.8646413417004553 1105 82-83 0.8488842017016829 1105 84-85 0.9818997638089648 1105 86-87 1.1250501671823976 1105 88-89 1.1945751760944532 1105 90-91 1.2085738324068949 1105 92-93 1.4413492314719463 1105 94-95 1.4866033985412663 1105 96-97 1.7059007931250747 1105 98-99 1.9327568466629188 1105 100-101 2.222651703643418 1106 1 -0.03692086442391229 1106 2 -0.038666332831247985 1106 3 0.06268867041418957 1106 4 -0.02301022810551956 1106 5 -0.03919850391292812 1106 6 -0.08073602353478293 1106 7 -0.011266610857013859 1106 8 -0.13452258539078343 1106 9 -0.12213779106710376 1106 10-11 -0.02983550113861355 1106 12-13 -0.08485127604119924 1106 14-15 -0.0407924523498977 1106 16-17 0.011661746811483908 1106 18-19 -0.025096741361984698 1106 20-21 -0.06846090352774326 1106 22-23 -0.03525654206880091 1106 24-25 0.008985749685542999 1106 26-27 -0.0018502160592959171 1106 28-29 -0.07996580018332367 1106 30-31 0.0017090834187456494 1106 32-33 -0.036071398704613955 1106 34-35 -0.12035298725225374 1106 36-37 -0.10543574873295114 1106 38-39 -0.014125245687132804 1106 40-41 -0.013150487417952661 1106 42-43 0.02118217109545384 1106 44-45 0.23389786452776917 1106 46-47 -0.08229190217958404 1106 48-49 0.02410165559484767 1106 50-51 0.10678510869868063 1106 52-53 0.0027785438538927565 1106 54-55 -0.03827244651004946 1106 56-57 0.08918474924116282 1106 58-59 0.05207170307540565 1106 60-61 0.139475711834379 1106 62-63 0.11198284727664287 1106 64-65 0.11459590432464495 1106 66-67 0.18185550291813257 1106 68-69 -0.14401080848521275 1106 70-71 0.02081884336614337 1106 72-73 0.08657681773857817 1106 74-75 0.24751890297931212 1106 76-77 0.207012133477253 1106 78-79 0.49415889098660415 1106 80-81 0.487016740081998 1106 82-83 0.5716529281609546 1106 84-85 0.5813615697154582 1106 86-87 0.7848693878706108 1106 88-89 0.9854814646271279 1106 90-91 0.9171119153241563 1106 92-93 0.9885104471336454 1106 94-95 1.1943538988343647 1106 96-97 1.3030276452202436 1106 98-99 1.3945957691292818 1106 100-101 1.8828025159037978 1107 1 0.04072164859061189 1107 2 0.08523834592840984 1107 3 0.16437085749991098 1107 4 0.03534169739646842 1107 5 0.04747252787159795 1107 6 0.08319678598440561 1107 7 0.02113711404355456 1107 8 -0.0035949170104032646 1107 9 0.057405973281198897 1107 10-11 0.052155962630905606 1107 12-13 0.123528941891351 1107 14-15 0.13245180024492953 1107 16-17 0.13637235566459083 1107 18-19 0.15847990687418445 1107 20-21 0.17051555717002742 1107 22-23 0.0641917555352336 1107 24-25 0.12448474732514825 1107 26-27 0.09357449374120108 1107 28-29 0.05667006815178155 1107 30-31 0.005794106214359829 1107 32-33 -0.11648778244697411 1107 34-35 0.03378336609595323 1107 36-37 -0.03731768037843608 1107 38-39 -0.017137985364435337 1107 40-41 1.4048810505329357E-4 1107 42-43 -0.13919832167784563 1107 44-45 -0.1544302953325527 1107 46-47 0.07200167624703369 1107 48-49 -0.05139224940725029 1107 50-51 -0.16689323061648054 1107 52-53 -0.22350269907300202 1107 54-55 -0.23876311809151218 1107 56-57 -0.19767422511780097 1107 58-59 -0.16480392339984462 1107 60-61 -0.11860016038083643 1107 62-63 0.08780777371632098 1107 64-65 -0.03524363717630763 1107 66-67 0.05593680728001971 1107 68-69 0.2821350349525602 1107 70-71 0.2112640853876755 1107 72-73 0.03266599547970728 1107 74-75 0.1594300162460378 1107 76-77 0.12788224697031936 1107 78-79 0.22699831931783265 1107 80-81 0.25400674881223395 1107 82-83 0.5206610763809572 1107 84-85 0.5490766730232473 1107 86-87 0.779561727897125 1107 88-89 0.9031836342523789 1107 90-91 1.0307185535367225 1107 92-93 1.170962269166175 1107 94-95 1.0191728272787657 1107 96-97 1.1246345744406625 1107 98-99 1.5190048615081082 1107 100-101 1.9606248879411758 1108 1 0.056449201542747574 1108 2 0.09118722246097377 1108 3 0.19940202913718963 1108 4 0.0741987043246013 1108 5 0.00443881889277975 1108 6 0.04550140573007866 1108 7 0.03754459523650411 1108 8 0.052361393139953805 1108 9 -0.0308836008220581 1108 10-11 0.030420064203859454 1108 12-13 0.09004891850023 1108 14-15 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-1.8796083143783946 2316 90-91 -2.1030311876556453 2316 92-93 -2.541436455320838 2316 94-95 -2.417456625574655 2316 96-97 -2.7401998704033446 2316 98-99 -3.4050900609585746 2316 100-101 -3.9665957496518587 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 15.0 5 50.0 6 74.0 7 208.0 8 555.0 9 1037.0 10 1862.0 11 3038.0 12 4761.0 13 7081.0 14 9968.0 15 13450.0 16 17335.0 17 24092.0 18 23932.0 19 17850.0 20 15530.0 21 15469.0 22 15973.0 23 17329.0 24 19720.0 25 22866.0 26 27059.0 27 32342.0 28 39193.0 29 47187.0 30 57730.0 31 71020.0 32 88799.0 33 116879.0 34 161308.0 35 239816.0 36 368853.0 37 527308.0 38 364446.0 39 26423.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.0182419707377 19.22932245477415 15.294953725438573 22.457481849049575 2 15.862521205576607 20.1541716243555 40.29821956478991 23.685087605277978 3 19.901017115790395 24.14819937385827 29.82167666840942 26.129106841941912 4 13.196820912427626 17.378298340847543 37.16028281698918 32.26459792973565 5 13.73676453557881 37.372435908651234 34.292776929363924 14.598022626406026 6 31.817604301661152 36.59607066848124 17.186732601254455 14.399592428603148 7 28.14539755882266 33.01812949712213 20.43133214999981 18.405140794055395 8 26.24132442374928 34.97024237251209 19.470698724173225 19.317734479565402 9 25.962617935875876 17.09361512278578 20.54509712929842 36.39866981203993 10-11 24.96453956842624 26.842599006982947 26.985003101355808 21.20785832323501 12-13 25.85341438654182 24.23271124315421 27.57923870358745 22.334635666716515 14-15 22.516572154115515 25.69834659619431 26.32961517777288 25.4554660719173 16-17 21.663980491243095 28.797223817912716 27.32367365488846 22.21512203595573 18-19 21.590664356653004 26.928266410837793 30.114998023380345 21.366071209128858 20-21 22.087776909000098 26.02420765462102 30.599280252174175 21.288735184204704 22-23 21.85722682554051 26.11297849712755 30.398827275101713 21.630967402230226 24-25 21.657498886181845 26.276548082264455 30.496059926853547 21.569893104700157 26-27 21.24835394500088 26.67680948344341 30.25839183369225 21.816444737863463 28-29 21.430621547156953 26.985621676293597 30.122996403336224 21.460760373213226 30-31 21.55043726228218 26.493239185915463 30.16646313789503 21.78986041390733 32-33 21.230693348130448 26.530264098500794 30.094006668699603 22.145035884669156 34-35 21.927764955814744 26.74780229887069 29.886985488787605 21.437447256526962 36-37 22.035054902822576 26.285323870932803 29.860469863594375 21.81915136265025 38-39 21.505222897877612 26.727224963245654 29.751487100407843 22.016065038468884 40-41 22.11632010633267 26.47618869048735 29.753272375380984 21.654218827798992 42-43 21.74039305649359 26.49521085048912 29.693310273307965 22.07108581970932 44-45 21.798445549661004 26.81714294760646 29.711323507478543 21.673087995253994 46-47 22.137309975869787 27.05122957862211 28.923055132903635 21.88840531260447 48-49 21.860246271857154 27.167011343401963 28.86509535176918 22.107647032971702 50-51 22.11139636826861 27.343898077242713 28.75660834562559 21.78809720886309 52-53 22.4075560608074 27.07521110672788 28.663234416259854 21.853998416204863 54-55 22.018084924244853 27.298991944806282 28.798890093698855 21.88403303725001 56-57 22.051972808045445 27.437834374686272 28.56771515681946 21.94247766044882 58-59 21.986529826544857 27.16139922176589 28.528203592240654 22.323867359448595 60-61 21.733380877735765 27.36054000665677 28.786018113250933 22.120061002356532 62-63 21.844875556275756 27.315342275957764 28.960977070795472 21.878805096971004 64-65 21.621907027643097 27.345397725450237 28.778853127370535 22.253842119536124 66-67 21.514456222261657 27.503209670418293 28.560359716365944 22.4219743909541 68-69 22.006057337883107 27.160522369889183 28.703308541196332 22.130111751031375 70-71 21.855706348885658 27.29580519236529 28.31744053374146 22.53104792500759 72-73 21.85404007309952 27.27866338021539 28.416250687859474 22.451045858825616 74-75 21.888427839635952 27.711228574296946 28.105406939955337 22.294936646111765 76-77 21.863121276133974 27.31938299473915 28.377155692227195 22.440340036899677 78-79 21.99886026736228 27.590194466881314 28.224045775928396 22.18689948982801 80-81 22.093629461644028 27.664145656104612 28.036309050581355 22.205915831670005 82-83 22.04987178902374 27.704193501824903 28.133097802552804 22.11283690659855 84-85 22.04301097665986 27.780522625483396 28.084922995912866 22.09154340194388 86-87 22.107408064665286 27.72910829452966 27.898923828162424 22.264559812642624 88-89 22.05167875342472 27.889501662319766 27.61112923187538 22.447690352380132 90-91 21.972156569255414 27.87638278270589 27.8451179222636 22.306342725775096 92-93 22.05842380408953 27.47502598888461 28.03117041522437 22.435379791801488 94-95 21.796684940180626 27.48573249575099 28.05624520945113 22.661337354617256 96-97 22.12236576761143 27.126162803049457 28.04347074449805 22.70800068484106 98-99 21.838501332085848 27.43936463118895 27.693659621292294 23.02847441543291 100-101 22.17099454343641 27.035890595452162 27.91503949067352 22.878075370437912 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 988.0 1 671.0 2 527.0 3 735.5 4 574.0 5 470.5 6 983.5 7 2384.5 8 3941.0 9 4544.0 10 3777.0 11 2689.5 12 2470.5 13 2579.0 14 2785.0 15 3023.5 16 3257.5 17 3543.0 18 3825.5 19 4206.0 20 4788.0 21 5659.0 22 6855.0 23 8388.5 24 10282.0 25 12961.0 26 16743.5 27 21391.0 28 25243.0 29 28462.5 30 32150.0 31 37566.5 32 44889.5 33 48607.0 34 50072.0 35 53135.5 36 57244.5 37 61574.0 38 64948.0 39 67918.5 40 71309.5 41 75375.5 42 77481.5 43 79162.0 44 81960.5 45 85176.5 46 85875.5 47 86239.0 48 85980.5 49 83969.5 50 83634.0 51 82103.0 52 79709.5 53 78951.0 54 79021.0 55 73884.0 56 66899.5 57 61102.0 58 56057.0 59 49339.5 60 39340.5 61 32345.5 62 27092.5 63 19718.0 64 13518.0 65 10393.5 66 7757.5 67 5737.5 68 4246.5 69 3086.5 70 2292.5 71 1556.5 72 1100.5 73 890.0 74 674.0 75 449.0 76 305.5 77 183.5 78 110.0 79 74.0 80 44.5 81 24.5 82 15.0 83 9.0 84 5.0 85 2.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010455880558019099 3 0.006165220408712456 4 0.0 5 2.0828447326731272E-4 6 8.331378930692508E-5 7 0.0 8 8.331378930692508E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0015204766548513827 26-27 0.02109921714197878 28-29 2.0828447326731272E-4 30-31 6.248534198019382E-5 32-33 0.003957404992078942 34-35 0.016017075994256346 36-37 0.007727353958217302 38-39 0.02109921714197878 40-41 0.022827978270097474 42-43 0.018745602594058143 44-45 0.009539428875642922 46-47 0.0018953887067325458 48-49 1.8745602594058143E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.0828447326731272E-4 68-69 1.457991312871189E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.8745602594058143E-4 82-83 0.00114556460297022 84-85 0.004415630833267029 86-87 7.081672091088631E-4 88-89 8.331378930692508E-5 90-91 0.004353145491286835 92-93 7.289956564355944E-4 94-95 1.2497068396038764E-4 96-97 2.499413679207753E-4 98-99 1.8745602594058143E-4 100-101 0.002478585231881021 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2400563.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.1790484908864 #Duplication Level Percentage of deduplicated Percentage of total 1 77.50128433170215 45.08950979240075 2 13.489983527872685 15.696688116187271 3 4.10376487174031 7.162594064045271 4 1.7122912567134632 3.984779042194135 5 0.9066555292725905 2.6374178001040156 6 0.5164697045162603 1.8028629589875167 7 0.3523250651778644 1.434855593607638 8 0.23726547991824357 1.104310388910154 9 0.1693983510168096 0.8869891390274847 >10 0.9095720093369173 9.6469252737461 >50 0.06238129522631116 2.5015875827887393 >100 0.03323310008818447 3.5746673858667277 >500 0.0028669212897608266 1.1664791032628057 >1k 0.0022935370318067526 2.1311782544013056 >5k 7.167303224396102E-5 0.2669967004428097 >10k+ 1.4334606448792203E-4 0.9121588040272469 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11635 0.48467796929303664 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10240 0.42656660125145646 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6403 0.26672909646612064 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2808 0.11697256018692283 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2779 0.1157645102419724 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2671 0.11126556561939845 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2541 0.10585016931444831 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2434 0.10139288158652782 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.165689465346254E-5 2 0.0 0.0 0.0 0.0 4.165689465346254E-5 3 0.0 0.0 0.0 0.0 4.165689465346254E-5 4 0.0 0.0 0.0 0.0 4.165689465346254E-5 5 0.0 0.0 0.0 0.0 4.165689465346254E-5 6 0.0 0.0 0.0 0.0 4.165689465346254E-5 7 4.165689465346254E-5 0.0 0.0 0.0 4.165689465346254E-5 8 4.165689465346254E-5 0.0 0.0 0.0 4.165689465346254E-5 9 4.165689465346254E-5 0.0 0.0 8.331378930692508E-5 4.165689465346254E-5 10-11 4.165689465346254E-5 0.0 0.0 8.331378930692508E-5 8.331378930692508E-5 12-13 4.165689465346254E-5 0.0 0.0 1.2497068396038764E-4 1.2497068396038764E-4 14-15 4.165689465346254E-5 0.0 0.0 1.2497068396038764E-4 1.6662757861385017E-4 16-17 4.165689465346254E-5 0.0 0.0 1.2497068396038764E-4 2.0828447326731272E-4 18-19 4.165689465346254E-5 0.0 0.0 1.2497068396038764E-4 2.0828447326731272E-4 20-21 4.165689465346254E-5 0.0 0.0 1.2497068396038764E-4 2.915982625742378E-4 22-23 4.165689465346254E-5 0.0 0.0 2.29112920594044E-4 2.915982625742378E-4 24-25 4.165689465346254E-5 0.0 0.0 5.623680778217443E-4 2.915982625742378E-4 26-27 8.331378930692508E-5 0.0 0.0 0.0012913637342573388 3.3325515722770033E-4 28-29 8.331378930692508E-5 0.0 0.0 0.004082375676039329 3.3325515722770033E-4 30-31 8.331378930692508E-5 4.165689465346254E-5 0.0 0.011393160687722005 3.3325515722770033E-4 32-33 8.331378930692508E-5 8.331378930692508E-5 0.0 0.02618135828970121 3.3325515722770033E-4 34-35 8.331378930692508E-5 1.2497068396038764E-4 0.0 0.049842474452867935 3.3325515722770033E-4 36-37 8.331378930692508E-5 1.6662757861385017E-4 0.0 0.08750030721959806 3.3325515722770033E-4 38-39 1.2497068396038764E-4 1.6662757861385017E-4 0.0 0.14357048742315864 3.3325515722770033E-4 40-41 1.2497068396038764E-4 1.6662757861385017E-4 0.0 0.20347310193483778 3.3325515722770033E-4 42-43 1.457991312871189E-4 1.6662757861385017E-4 0.0 0.2630841181839427 3.3325515722770033E-4 44-45 2.0828447326731272E-4 1.6662757861385017E-4 0.0 0.3351297174871062 3.540836045544316E-4 46-47 2.707698152475065E-4 1.6662757861385017E-4 0.0 0.4237755893096744 3.7491205188116286E-4 48-49 3.3325515722770033E-4 1.6662757861385017E-4 0.0 0.5160039540724406 4.1656894653462545E-4 50-51 3.3325515722770033E-4 1.8745602594058146E-4 0.0 0.6138559996134241 4.1656894653462545E-4 52-53 3.3325515722770033E-4 2.0828447326731272E-4 0.0 0.7066259040066851 4.1656894653462545E-4 54-55 3.3325515722770033E-4 2.0828447326731272E-4 0.0 0.8054152296773716 4.1656894653462545E-4 56-57 3.7491205188116286E-4 2.0828447326731272E-4 0.0 0.9247622328595417 4.1656894653462545E-4 58-59 3.7491205188116286E-4 2.0828447326731272E-4 0.0 1.049691259925276 4.1656894653462545E-4 60-61 3.7491205188116286E-4 2.499413679207753E-4 0.0 1.1844304856819003 4.1656894653462545E-4 62-63 3.7491205188116286E-4 2.499413679207753E-4 0.0 1.3163578710494164 4.1656894653462545E-4 64-65 4.1656894653462545E-4 2.499413679207753E-4 0.0 1.454804560430199 4.1656894653462545E-4 66-67 4.998827358415506E-4 2.499413679207753E-4 0.0 1.6101847774876146 4.1656894653462545E-4 68-69 4.998827358415506E-4 2.499413679207753E-4 0.0 1.7836232583773057 4.1656894653462545E-4 70-71 5.623680778217443E-4 2.499413679207753E-4 0.0 1.9629770183077886 4.373973938613567E-4 72-73 5.831965251484756E-4 2.499413679207753E-4 0.0 2.1484126848576772 4.998827358415506E-4 74-75 5.831965251484756E-4 2.499413679207753E-4 0.0 2.3320571049374665 5.41539630495013E-4 76-77 5.831965251484756E-4 2.499413679207753E-4 0.0 2.5325309104572553 5.41539630495013E-4 78-79 5.831965251484756E-4 2.499413679207753E-4 0.0 2.7581029950057547 6.248534198019381E-4 80-81 6.248534198019381E-4 2.499413679207753E-4 0.0 2.9942142739015805 6.456818671286694E-4 82-83 6.248534198019381E-4 2.499413679207753E-4 0.0 3.2392817851479005 7.081672091088633E-4 84-85 6.248534198019381E-4 2.499413679207753E-4 0.0 3.4870153376520427 7.914809984157883E-4 86-87 6.248534198019381E-4 2.499413679207753E-4 0.0 3.7388937511742038 8.123094457425196E-4 88-89 6.248534198019381E-4 2.499413679207753E-4 0.0 4.012683691284086 8.331378930692509E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4800 0.0 61.158596 1 GTATCAA 8575 0.0 42.37772 1 TGGTATC 1790 0.0 35.295383 2 GTGGTAT 2000 0.0 33.251274 1 ATCAACG 11040 0.0 32.31194 3 TATCAAC 11140 0.0 32.151814 2 TCAACGC 11260 0.0 31.84737 4 CAACGCA 11475 0.0 31.209278 5 AACGCAG 11950 0.0 29.96874 6 ACGCAGA 13555 0.0 26.280087 7 CGCAGAG 13685 0.0 26.030441 8 TAAGGTG 1755 0.0 24.898647 5 GCAGAGT 15140 0.0 23.277376 9 GTAAGGT 1705 0.0 22.564497 4 GGTAAGG 1670 0.0 22.469984 3 GAGTACT 10515 0.0 21.975035 12-13 GTACATG 8200 0.0 21.89718 1 TACATGG 8180 0.0 21.71889 2 CAGAGTA 14690 0.0 21.436195 10-11 GTGTACG 180 5.170903E-6 21.111921 1 >>END_MODULE