FastQCFastQC Report
Thu 26 May 2016
SRR938241_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938241_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2400331
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123760.5155955574460356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101980.42485807165761724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61020.2542149395229241No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27450.11435922795647767No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26610.11085971059824665No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC25510.10627700929580128No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24930.10386067588178463No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24490.10202759536080648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49650.058.8516271
GTATCAA93500.043.9150241
TGGTATC20600.038.290242
GTGGTAT22250.035.6208571
ATCAACG119300.034.134753
TATCAAC119850.034.0566862
TCAACGC121600.033.5281834
CAACGCA123900.032.905795
AACGCAG127600.031.9888656
ACGCAGA143000.028.4590597
CGCAGAG143700.028.254268
GCAGAGT155050.025.4183549
GAGTACT107050.023.60105712-13
TAAGGTG15500.022.379355
CAGAGTA150450.022.30861910-11
GTACTTT115200.021.75269914-15
AGAGTAC144300.021.67802610-11
GTGTAAG12450.021.092091
GTAAGGT16300.020.9894584
GTACATG80950.020.9381261
Produced by FastQC (version 0.11.5)