##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938241_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2400331 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.878753805204365 33.0 31.0 34.0 30.0 34.0 2 32.074715528816654 34.0 31.0 34.0 30.0 34.0 3 32.140218578187756 34.0 31.0 34.0 30.0 34.0 4 35.65673567520479 37.0 35.0 37.0 35.0 37.0 5 35.67787276004851 37.0 35.0 37.0 35.0 37.0 6 35.70808900939079 37.0 36.0 37.0 35.0 37.0 7 35.653631103376995 37.0 35.0 37.0 33.0 37.0 8 35.63628807860249 37.0 35.0 37.0 33.0 37.0 9 37.29737981969986 39.0 37.0 39.0 34.0 39.0 10-11 37.2173714791835 39.0 37.0 39.0 34.0 39.0 12-13 37.12328966296731 39.0 37.0 39.0 34.0 39.0 14-15 38.36911700927914 40.0 38.0 41.0 33.5 41.0 16-17 38.2859384809845 40.0 38.0 41.0 33.0 41.0 18-19 38.23734789077007 40.0 38.0 41.0 33.5 41.0 20-21 38.13661886631469 40.0 38.0 41.0 33.0 41.0 22-23 37.98455692152457 40.0 38.0 41.0 33.0 41.0 24-25 37.9341978252166 40.0 38.0 41.0 33.0 41.0 26-27 37.938944045633704 40.0 38.0 41.0 33.0 41.0 28-29 37.84907269039145 40.0 38.0 41.0 33.0 41.0 30-31 37.6437318436499 40.0 37.5 41.0 32.5 41.0 32-33 37.39519841221898 40.0 37.0 41.0 32.0 41.0 34-35 37.17641004511461 40.0 36.5 41.0 31.0 41.0 36-37 36.93834037888941 40.0 36.0 41.0 30.5 41.0 38-39 36.68299726162767 39.5 36.0 41.0 30.0 41.0 40-41 36.43813249089396 39.0 35.5 41.0 30.0 41.0 42-43 36.139758433316075 39.0 35.0 41.0 29.5 41.0 44-45 35.883891013364405 39.0 35.0 41.0 28.5 41.0 46-47 35.69405969426717 39.0 35.0 41.0 27.0 41.0 48-49 35.414101638482364 39.0 35.0 41.0 26.0 41.0 50-51 34.76513010080693 38.5 34.5 40.5 24.5 40.5 52-53 34.841138784609285 38.5 34.5 40.0 25.5 41.0 54-55 34.974365410437144 39.0 35.0 40.0 25.0 41.0 56-57 34.76111461294296 38.5 35.0 40.0 24.0 41.0 58-59 34.49481800634996 38.0 34.0 40.0 23.5 41.0 60-61 34.12377063829947 38.0 34.0 40.0 22.5 41.0 62-63 33.70025925591096 37.0 34.0 40.0 21.0 41.0 64-65 33.274992074009795 37.0 33.0 40.0 19.5 41.0 66-67 32.848983327716056 36.0 33.0 39.0 18.0 41.0 68-69 32.357901681059815 35.5 32.0 39.0 13.5 40.5 70-71 31.874338580804064 35.0 31.5 38.5 8.5 40.0 72-73 31.384120981647946 35.0 31.0 37.5 7.0 40.0 74-75 30.85869657143119 35.0 31.0 37.0 3.5 39.0 76-77 30.31063299186654 35.0 30.0 36.5 2.0 39.0 78-79 29.73534774995615 34.0 29.5 36.0 2.0 38.5 80-81 29.192145166645766 34.0 29.0 35.5 2.0 37.0 82-83 28.635529433232335 34.0 28.5 35.0 2.0 37.0 84-85 28.124591358441815 34.0 27.0 35.0 2.0 36.0 86-87 27.611252989691838 33.5 26.5 35.0 2.0 36.0 88-89 27.03934894812424 33.0 25.5 35.0 2.0 35.5 90-91 26.421620601492044 33.0 24.5 35.0 2.0 35.0 92-93 25.802463910185722 32.0 23.0 35.0 2.0 35.0 94-95 25.179293605756875 32.0 19.5 34.0 2.0 35.0 96-97 24.323890746734513 31.5 9.0 34.0 2.0 35.0 98-99 23.23248751942961 31.0 2.0 34.0 2.0 35.0 100-101 20.347998255240633 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4276640950017132 1101 2 0.42372017886297186 1101 3 0.3881887521042202 1101 4 0.29638492316257015 1101 5 0.2740681171624786 1101 6 0.21258044585704283 1101 7 0.36513967122468927 1101 8 0.3108895628428243 1101 9 0.30845909885631784 1101 10-11 0.3771247741353534 1101 12-13 0.2550954720289269 1101 14-15 0.34247551867832016 1101 16-17 0.487366885641066 1101 18-19 0.3119025419559094 1101 20-21 0.5460543104934104 1101 22-23 0.5128234638612668 1101 24-25 0.3966721540611786 1101 26-27 0.4074749078804629 1101 28-29 0.5199263926182596 1101 30-31 0.4922909016261201 1101 32-33 0.5638420879970312 1101 34-35 0.5628933442095843 1101 36-37 0.5503442186350327 1101 38-39 0.5504401887001364 1101 40-41 0.6975071002999016 1101 42-43 0.6814859936363504 1101 44-45 0.6631713729743396 1101 46-47 0.7028729692384488 1101 48-49 0.9675836838615552 1101 50-51 0.9448472047154937 1101 52-53 0.992502110966619 1101 54-55 1.0124724526694706 1101 56-57 1.0468561098665745 1101 58-59 0.9341159007225528 1101 60-61 0.8661468719979908 1101 62-63 1.03007412189055 1101 64-65 1.0117228959209115 1101 66-67 0.8580350222585906 1101 68-69 0.9099672067786813 1101 70-71 1.0314649962668092 1101 72-73 1.1896066702813108 1101 74-75 1.1239111338592096 1101 76-77 1.0516671775450597 1101 78-79 1.1721745612763605 1101 80-81 1.1493907995045092 1101 82-83 1.1497431483678113 1101 84-85 1.2190577904820685 1101 86-87 1.1543210734310883 1101 88-89 1.0922738067533544 1101 90-91 1.283026158711614 1101 92-93 1.392222485919202 1101 94-95 1.539172316567555 1101 96-97 1.5466029499820557 1101 98-99 1.863966927590834 1101 100-101 1.7996920873363322 1102 1 0.39915350029290053 1102 2 0.1818046618136293 1102 3 0.19914381241161294 1102 4 0.22027368675933445 1102 5 0.0925066309239142 1102 6 0.23861515468489358 1102 7 0.18638756796751466 1102 8 0.23764861629883427 1102 9 0.3197498710596989 1102 10-11 0.38120170165169753 1102 12-13 0.26999284713414795 1102 14-15 0.25282464073548994 1102 16-17 0.3006398146847715 1102 18-19 0.35654589917709245 1102 20-21 0.43307364927897396 1102 22-23 0.40778965947924917 1102 24-25 0.4765974174887475 1102 26-27 0.38177997850582557 1102 28-29 0.3741623914367551 1102 30-31 0.3274494181645693 1102 32-33 0.41793934265145083 1102 34-35 0.338628789435667 1102 36-37 0.3754238181744398 1102 38-39 0.28737230926252977 1102 40-41 0.258598083036631 1102 42-43 0.215649854007971 1102 44-45 0.26374310967006664 1102 46-47 0.37708207958701223 1102 48-49 0.42662029464509743 1102 50-51 0.5403397124241565 1102 52-53 0.5196691340237081 1102 54-55 0.5153060067369992 1102 56-57 0.4222701328872489 1102 58-59 0.603474289356555 1102 60-61 0.5175540231895184 1102 62-63 0.5383668275678559 1102 64-65 0.3818823164792917 1102 66-67 0.37183011065129534 1102 68-69 0.50371982220733 1102 70-71 0.4813368256219128 1102 72-73 0.5457735874967256 1102 74-75 0.5391940324643798 1102 76-77 0.4973445248503445 1102 78-79 0.39607452662758647 1102 80-81 0.6630375604831329 1102 82-83 0.7780082415074183 1102 84-85 0.7139919780450086 1102 86-87 0.5506513914480458 1102 88-89 0.6621297364318934 1102 90-91 0.7743452249282505 1102 92-93 0.7519028355502364 1102 94-95 0.9019426254442244 1102 96-97 0.8305936197298429 1102 98-99 0.9444903987802817 1102 100-101 0.8887890534284502 1103 1 0.4516923887688158 1103 2 0.30764275477572767 1103 3 0.27161242404986297 1103 4 0.2120060523782783 1103 5 0.13566510357507156 1103 6 0.1337382681630075 1103 7 0.2786562762215681 1103 8 0.24785901062769966 1103 9 0.2627397882441045 1103 10-11 0.36124863342410407 1103 12-13 0.20917639238538754 1103 14-15 0.4342343304529592 1103 16-17 0.32709683856984384 1103 18-19 0.43246937569144706 1103 20-21 0.4432608009332242 1103 22-23 0.5354775416906961 1103 24-25 0.551528626339227 1103 26-27 0.36778092582400035 1103 28-29 0.3676203464622745 1103 30-31 0.3369176851559388 1103 32-33 0.4429750821033309 1103 34-35 0.5618181594854619 1103 36-37 0.4312840541357019 1103 38-39 0.5036305499169771 1103 40-41 0.5574189115038592 1103 42-43 0.47862085349827765 1103 44-45 0.46614206086229615 1103 46-47 0.4982967233321034 1103 48-49 0.5373160410718114 1103 50-51 0.5311763647499887 1103 52-53 0.5335856598520508 1103 54-55 0.6524660467840206 1103 56-57 0.5263575875973032 1103 58-59 0.7276122881807012 1103 60-61 0.7107495919684794 1103 62-63 0.7103751113799603 1103 64-65 0.5931259756831082 1103 66-67 0.6761376437966788 1103 68-69 0.7906186944563771 1103 70-71 0.8620121082559145 1103 72-73 1.0264248288091302 1103 74-75 0.9133731411293127 1103 76-77 1.0825788939468843 1103 78-79 0.9618290770210827 1103 80-81 0.9631416508047828 1103 82-83 0.9360503400321925 1103 84-85 1.1565430489589232 1103 86-87 1.019233541196808 1103 88-89 1.0711323103277266 1103 90-91 1.2013530899626481 1103 92-93 1.3823137967546266 1103 94-95 1.2479609300231935 1103 96-97 1.1364548284021687 1103 98-99 1.1435315514203523 1103 100-101 1.1477996854674224 1104 1 0.41667529117173885 1104 2 0.32436951180162765 1104 3 0.2832195441788983 1104 4 0.21772755709024239 1104 5 0.26755052151933256 1104 6 0.25850965095865064 1104 7 0.31635005605944144 1104 8 0.31924176770297663 1104 9 0.2911247692026322 1104 10-11 0.34707542739902664 1104 12-13 0.26261400061938645 1104 14-15 0.36557328481899987 1104 16-17 0.4813060371002109 1104 18-19 0.495228896233634 1104 20-21 0.42152519619973816 1104 22-23 0.43990937924462514 1104 24-25 0.4518191273570622 1104 26-27 0.4878455518868634 1104 28-29 0.5879232512410368 1104 30-31 0.5500160142736874 1104 32-33 0.6146476073871696 1104 34-35 0.7209826913630479 1104 36-37 0.7244247280579543 1104 38-39 0.6544461666222503 1104 40-41 0.6670131395895851 1104 42-43 0.7049925760088556 1104 44-45 0.7812868755425484 1104 46-47 0.915714712333795 1104 48-49 1.0116543143109311 1104 50-51 0.9076737061951334 1104 52-53 0.9590077579852405 1104 54-55 0.8770301409816383 1104 56-57 0.8629153780925378 1104 58-59 0.8845294097676444 1104 60-61 0.9058889838602155 1104 62-63 0.8879892734202812 1104 64-65 0.8069021533654421 1104 66-67 0.9145316807348891 1104 68-69 1.0384034892042138 1104 70-71 0.8921358720259782 1104 72-73 0.9501684080501143 1104 74-75 0.8763370576013969 1104 76-77 0.7488919801460803 1104 78-79 0.7457097417330765 1104 80-81 0.8480471106965908 1104 82-83 1.0019533230287045 1104 84-85 0.9575167789040542 1104 86-87 1.052854830000026 1104 88-89 0.9841747530972498 1104 90-91 0.970123709865284 1104 92-93 0.875474757922678 1104 94-95 0.9486210754588349 1104 96-97 0.9581890446549508 1104 98-99 1.1227233360885975 1104 100-101 0.9228761290438108 1105 1 0.36161466955388377 1105 2 0.34545477609635356 1105 3 0.33026906681332235 1105 4 0.1872697060478643 1105 5 0.2719952200218785 1105 6 0.19003440220940604 1105 7 0.1729527144658647 1105 8 0.21772482514359126 1105 9 0.21707198647551706 1105 10-11 0.36957233196473993 1105 12-13 0.2894835206940485 1105 14-15 0.3434923404706609 1105 16-17 0.3670997912975622 1105 18-19 0.3287772202411148 1105 20-21 0.3790834126543601 1105 22-23 0.3112637054465921 1105 24-25 0.4097629249120658 1105 26-27 0.3942624123327505 1105 28-29 0.4020943723131438 1105 30-31 0.6250180994675176 1105 32-33 0.6889646345047566 1105 34-35 0.5981671116576024 1105 36-37 0.6371863300045177 1105 38-39 0.6684964183554811 1105 40-41 0.7458911686931344 1105 42-43 0.7775418620816339 1105 44-45 0.8625497552096064 1105 46-47 0.8383414381912075 1105 48-49 0.8013465181307211 1105 50-51 0.8897669244257713 1105 52-53 0.949376858864035 1105 54-55 0.8943464174294533 1105 56-57 0.9921504786635893 1105 58-59 0.9317412539562113 1105 60-61 0.945500835814336 1105 62-63 0.9139731253916494 1105 64-65 1.1382410707040478 1105 66-67 1.1880008968026772 1105 68-69 1.0740958957911246 1105 70-71 1.1490373243275265 1105 72-73 1.1881898682897791 1105 74-75 1.0911150779327556 1105 76-77 1.0743499318529892 1105 78-79 1.1429672085245706 1105 80-81 1.1858504884229824 1105 82-83 1.405888263543158 1105 84-85 1.344911458639853 1105 86-87 1.4746970915581095 1105 88-89 1.3559319171761217 1105 90-91 1.3348379520267528 1105 92-93 1.5204600824723151 1105 94-95 1.8202968091234943 1105 96-97 1.802811006203978 1105 98-99 1.8422038280217734 1105 100-101 1.9088540993305152 1106 1 0.15334324223272588 1106 2 0.13223542246949194 1106 3 0.07790355088645384 1106 4 0.17261428283904934 1106 5 0.1902301627212637 1106 6 0.18024249980983598 1106 7 0.27382835230678637 1106 8 0.25498743036074956 1106 9 0.3007256167593795 1106 10-11 -0.36329733038214584 1106 12-13 -0.03836766411351533 1106 14-15 0.025182868409743264 1106 16-17 0.18933818355273502 1106 18-19 0.22908248102123707 1106 20-21 0.1529842645618018 1106 22-23 0.08168516561325845 1106 24-25 0.1804800637594539 1106 26-27 0.1861958585987935 1106 28-29 0.14706351068869594 1106 30-31 0.1665757856084511 1106 32-33 0.11311315081871953 1106 34-35 0.04995886046683751 1106 36-37 -0.11836540693260389 1106 38-39 -0.043998137662960346 1106 40-41 0.2765595080184511 1106 42-43 -0.006568336212474435 1106 44-45 7.00710980787278E-4 1106 46-47 0.312617133524725 1106 48-49 0.2850260754308067 1106 50-51 0.38415171040421825 1106 52-53 0.3391966392921333 1106 54-55 -0.0668688245427731 1106 56-57 0.13836907782985008 1106 58-59 0.2186453087754998 1106 60-61 -0.04915039825283429 1106 62-63 0.15251378203182497 1106 64-65 0.07992716389532006 1106 66-67 -0.18568982347024132 1106 68-69 -0.4967110622659128 1106 70-71 -0.08668301258877165 1106 72-73 0.06535911549307016 1106 74-75 0.2313051701803559 1106 76-77 0.4672654741219837 1106 78-79 0.639973234869462 1106 80-81 0.591671510026984 1106 82-83 0.5537808762288172 1106 84-85 0.7372290709882385 1106 86-87 0.6239586126489307 1106 88-89 0.4006655330833766 1106 90-91 0.45794734673902227 1106 92-93 0.5025637822350468 1106 94-95 0.301818750920372 1106 96-97 0.4848996928579119 1106 98-99 0.5414390242835765 1106 100-101 0.3667198440862194 1107 1 0.21835978078972929 1107 2 0.24950139060555898 1107 3 0.07273973156819835 1107 4 0.1384588774784845 1107 5 0.15814008598820095 1107 6 0.1376343122949848 1107 7 0.07889371677224943 1107 8 0.07878642716320883 1107 9 0.05134813547807937 1107 10-11 0.13712326795258178 1107 12-13 0.08756213196846829 1107 14-15 0.1141048226141379 1107 16-17 0.1215755609570337 1107 18-19 0.16983519110139156 1107 20-21 0.1648257253703349 1107 22-23 0.17446293638567312 1107 24-25 0.18355267147576626 1107 26-27 0.22710970678652131 1107 28-29 0.14860819333024722 1107 30-31 0.2394345335892396 1107 32-33 0.3178702921968295 1107 34-35 0.42430125040777966 1107 36-37 0.38179262853635976 1107 38-39 0.3484973427715019 1107 40-41 0.32312812245636735 1107 42-43 0.41931253109282096 1107 44-45 0.31143104556136336 1107 46-47 0.4583352931523308 1107 48-49 0.313655050956136 1107 50-51 0.30900704780538746 1107 52-53 0.3324610099841081 1107 54-55 0.3922458649079843 1107 56-57 0.33793427704247136 1107 58-59 0.43756889891661643 1107 60-61 0.3275852542834983 1107 62-63 0.40339571120273376 1107 64-65 0.4189215406532085 1107 66-67 0.37304055358496413 1107 68-69 0.4412604724351965 1107 70-71 0.48301154176613537 1107 72-73 0.47040476907799444 1107 74-75 0.40769896835421093 1107 76-77 0.4773264849384127 1107 78-79 0.4758296328089102 1107 80-81 0.6081142502508499 1107 82-83 0.5060645343824213 1107 84-85 0.4278161134403007 1107 86-87 0.6251198622523937 1107 88-89 0.7031946149279946 1107 90-91 0.7524960936712297 1107 92-93 0.7998696466533879 1107 94-95 0.9925314751233394 1107 96-97 1.096446933931933 1107 98-99 1.149434656697558 1107 100-101 1.3616856055566906 1108 1 0.23101447361533545 1108 2 0.14390137688050686 1108 3 0.1509766845039593 1108 4 0.18298429552083917 1108 5 0.20448915738645468 1108 6 0.2519484661325393 1108 7 0.1869111831776351 1108 8 0.10494854549861543 1108 9 0.257142015627835 1108 10-11 0.2957509275000163 1108 12-13 0.14115943118233076 1108 14-15 0.10573253807846328 1108 16-17 0.3026459120388978 1108 18-19 0.35645370569442747 1108 20-21 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-1.5204447513677906 2316 86-87 -1.4863888471003115 2316 88-89 -1.4642711384966631 2316 90-91 -1.721361077320939 2316 92-93 -1.7717024133930046 2316 94-95 -1.6947321906826005 2316 96-97 -1.9429486632495632 2316 98-99 -1.8266233764767605 2316 100-101 -1.9230352793835692 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6685.0 3 1961.0 4 1973.0 5 2211.0 6 2500.0 7 3072.0 8 3776.0 9 4699.0 10 6137.0 11 7512.0 12 9667.0 13 11997.0 14 14703.0 15 17468.0 16 21225.0 17 27691.0 18 24693.0 19 19640.0 20 18308.0 21 18523.0 22 19917.0 23 21596.0 24 24070.0 25 26762.0 26 30559.0 27 35042.0 28 41237.0 29 48397.0 30 57873.0 31 70093.0 32 87266.0 33 114141.0 34 156923.0 35 228840.0 36 343276.0 37 470009.0 38 358215.0 39 41670.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.71468264203146 18.8179340999454 14.89421121915436 22.573172038868773 2 15.645804260512483 19.834707988002407 40.88501418003391 23.6344735714512 3 19.630610621726667 24.250166324014184 29.631352618581936 26.48787043567721 4 12.9406188288351 17.049621950034954 37.5073423443892 32.502416876740746 5 13.787263352849106 37.269898874333826 34.24242474781125 14.700413025005815 6 31.71494824292876 36.49532476415291 17.32510614161669 14.464620851301643 7 28.137117528535928 33.01829409378553 20.498423483611358 18.346164894067186 8 26.152994544989973 34.84457994088565 19.624280817763818 19.37814469636056 9 26.186746651483332 17.10104779998683 20.590657748959828 36.12154779957001 10-11 24.922936900920003 26.7385970899156 27.08485206407364 21.25361394509076 12-13 25.919914819149177 24.287073313753638 27.54770986794966 22.245301999147525 14-15 22.62715425248106 25.64427489062 26.363665163803223 25.364905693095718 16-17 21.659504949421933 28.741226062647936 27.25479455709489 22.344474430835238 18-19 21.566593000175708 26.90244946119524 30.114101425480992 21.41685611314806 20-21 22.163994585865172 26.07310749700911 30.54819053201817 21.21470738510754 22-23 21.885952168245314 26.14950998068604 30.334319527860586 21.630218323208062 24-25 21.64579252116812 26.254575340820672 30.472082602571447 21.627549535439762 26-27 21.254972161316164 26.63373396886395 30.26194480788901 21.849349061930884 28-29 21.409446601502598 26.99900300765264 30.08448900442299 21.507061386421768 30-31 21.574036205029813 26.52943341934123 30.08266847103977 21.813861904589185 32-33 21.200894430778025 26.56885488971682 30.04414412367442 22.186106555830737 34-35 21.970388822588294 26.77008864592182 29.793618118997117 21.46590441249277 36-37 22.054790066048742 26.238148183815866 29.8151110421844 21.89195070795099 38-39 21.508043265568947 26.733128626606806 29.67855070138276 22.08027740644149 40-41 22.131910575840422 26.514425446419548 29.691143338774538 21.66252063896549 42-43 21.76747995265325 26.55919177100178 29.57521297701015 22.09811529933481 44-45 21.841497543153167 26.825342191760733 29.643215502470184 21.689944762615927 46-47 22.137976385791802 27.091934388956425 28.825119590008068 21.9449696352437 48-49 21.889808766299996 27.170775138347985 28.808072658762896 22.131343436589127 50-51 22.071651320769497 27.41815342091166 28.681968322534495 21.828226935784343 52-53 22.390820279577927 27.095964557598172 28.6771460640586 21.836069098765297 54-55 21.9867511309502 27.299883487960425 28.783849331097542 21.929516049991832 56-57 22.005505157934195 27.477534634344124 28.524185545199614 21.992774662522066 58-59 22.001483444591678 27.13726446150128 28.531681542782376 22.329570551124668 60-61 21.743174491849146 27.323014773156075 28.8332309228425 22.100579812152283 62-63 21.858756878520516 27.276839251590303 28.997167337121883 21.8672365327673 64-65 21.57312290022658 27.333427090658123 28.85187905305102 22.241570956064276 66-67 21.552255037583958 27.374477074617907 28.67724878747979 22.396019100318338 68-69 22.038170035080732 27.03880964693009 28.82101410354545 22.10200621444373 70-71 21.824710948990898 27.215182487349526 28.42856946372655 22.531537099933022 72-73 21.835302972713453 27.245205515990506 28.460455509322237 22.459036001973807 74-75 21.775714157416875 27.64535642702606 28.321230759741844 22.257698655815226 76-77 21.900509959588266 27.263211320286125 28.348240143545418 22.4880385765802 78-79 22.009601774686633 27.45162133073997 28.284026528631777 22.254750365941618 80-81 22.110315947373355 27.559465399960416 28.0201464629103 22.31007218975593 82-83 22.079795051551717 27.540781132608526 28.13557499865615 22.2438488171836 84-85 22.055437043352132 27.63316372164015 28.099996937364097 22.211402297643627 86-87 22.199325199844342 27.57656081909306 27.85625540077613 22.36785858028647 88-89 22.071005892497965 27.70026282424785 27.74525470111973 22.483476582134454 90-91 22.06307767850667 27.652621338017426 27.838257937960236 22.44604304551567 92-93 22.14023624214664 27.288537190311686 28.030161746292347 22.541064821249332 94-95 21.956056332861753 27.164857738098714 28.15669775984087 22.722388169198666 96-97 22.274242793989572 26.815113548339504 28.126953072493677 22.78369058517725 98-99 21.97281076117241 27.102221260917048 27.768939187931895 23.156028789978645 100-101 22.439460030735557 26.46316793236091 28.087394979024495 23.009977057879038 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1028.0 1 703.5 2 550.5 3 777.5 4 638.5 5 557.5 6 1071.5 7 2280.5 8 3829.0 9 4511.0 10 3707.5 11 2726.0 12 2533.5 13 2685.0 14 2926.0 15 3127.5 16 3324.0 17 3535.0 18 3930.0 19 4485.0 20 5001.5 21 5801.0 22 6979.5 23 8559.0 24 10568.0 25 13222.0 26 17023.0 27 21591.0 28 25373.5 29 28572.5 30 32043.5 31 37355.5 32 44710.5 33 48535.0 34 50021.0 35 52835.5 36 57145.0 37 61437.5 38 64464.5 39 67317.5 40 70739.5 41 74991.0 42 76964.0 43 78515.5 44 81337.0 45 84714.0 46 85292.5 47 85285.0 48 85951.0 49 83904.5 50 82482.5 51 81166.5 52 79192.5 53 78724.0 54 78537.0 55 73647.5 56 66874.0 57 60970.5 58 56159.0 59 49605.5 60 39790.5 61 32915.0 62 27728.0 63 20479.0 64 14433.0 65 11131.0 66 8268.0 67 6159.5 68 4695.5 69 3501.5 70 2670.5 71 1865.0 72 1294.0 73 1025.0 74 754.0 75 519.5 76 369.5 77 256.0 78 165.0 79 94.5 80 58.0 81 36.0 82 22.5 83 18.5 84 11.5 85 6.5 86 6.5 87 7.5 88 6.0 89 4.5 90 3.0 91 1.5 92 1.5 93 3.0 94 2.0 95 1.5 96 1.5 97 1.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4993894592037515 2 0.0492848694617534 3 0.05828362838291885 4 0.06557428954590012 5 0.08036391647651928 6 0.10794344613305415 7 0.07032363453207079 8 0.05215947300601459 9 0.05036805340596776 10-11 0.07538543642522635 12-13 0.10869334270981793 14-15 0.05645054786194071 16-17 0.06103324916438608 18-19 0.06067913133646984 20-21 0.07476052261125653 22-23 0.06449110560168576 24-25 0.08952931908141003 26-27 0.06667830395058015 28-29 0.0673448786854813 30-31 0.07140681847628515 32-33 0.07126100525302552 34-35 0.07263581564375912 36-37 0.06865719769481793 38-39 0.04014029731732832 40-41 0.043598153754628007 42-43 0.04211919106156609 44-45 0.021122086912180028 46-47 0.028079460707710727 48-49 0.028141952089107712 50-51 0.025517314070434453 52-53 0.025267348544846524 54-55 0.02412167321923518 56-57 0.024225825521563483 58-59 0.021288730595905317 60-61 0.028100291168176392 62-63 0.01953897191678981 64-65 0.021601187502890227 66-67 0.015456201665520298 68-69 0.018247483367918843 70-71 0.042244173824360055 72-73 0.03778645528470865 74-75 0.0321830614194459 76-77 0.02220527085639439 78-79 0.014914609693413117 80-81 0.01691433389811655 82-83 0.02116374783311135 84-85 0.018226652907453178 86-87 0.008873776158371491 88-89 0.04203586921970345 90-91 0.053700927080473485 92-93 0.033391228126454224 94-95 0.05436750181537463 96-97 0.044431372173254435 98-99 0.03397448101949273 100-101 0.17012237062305158 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2400331.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.72112707160009 #Duplication Level Percentage of deduplicated Percentage of total 1 78.72318191510159 47.01437150632473 2 12.872215877320187 15.374864802050144 3 3.8298659914928748 6.86171740635437 4 1.6423608853283695 3.923345725204848 5 0.8131423209479401 2.4280887938313884 6 0.4878243079280922 1.7480050489433379 7 0.3113900555640709 1.3017595554022143 8 0.21083584172767797 1.0073083276053136 9 0.16614609026725338 0.8930188592370161 >10 0.8463066085981255 9.206058476557331 >50 0.06125609155591688 2.5094905258801594 >100 0.030373326337940588 3.331939809207611 >500 0.002794897441011949 1.185426247960383 >1k 0.002096173080758255 2.0179954009909027 >5k 6.987243602527516E-5 0.2546279556833434 >10k+ 1.397448720505503E-4 0.9419815587669279 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12376 0.5155955574460356 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10198 0.42485807165761724 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6102 0.2542149395229241 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2745 0.11435922795647767 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2661 0.11085971059824665 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2551 0.10627700929580128 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2493 0.10386067588178463 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2449 0.10202759536080648 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.1660920931321556E-5 0.0 5 0.0 0.0 0.0 4.1660920931321556E-5 0.0 6 4.1660920931321556E-5 0.0 0.0 4.1660920931321556E-5 0.0 7 4.1660920931321556E-5 0.0 0.0 4.1660920931321556E-5 0.0 8 4.1660920931321556E-5 0.0 0.0 4.1660920931321556E-5 0.0 9 4.1660920931321556E-5 0.0 0.0 4.1660920931321556E-5 0.0 10-11 4.1660920931321556E-5 0.0 0.0 4.1660920931321556E-5 0.0 12-13 4.1660920931321556E-5 0.0 0.0 6.249138139698234E-5 1.2498276279396467E-4 14-15 4.1660920931321556E-5 0.0 0.0 8.332184186264311E-5 1.4581322325962545E-4 16-17 4.1660920931321556E-5 0.0 0.0 1.4581322325962545E-4 1.6664368372528622E-4 18-19 4.1660920931321556E-5 0.0 0.0 2.0830460465660777E-4 1.6664368372528622E-4 20-21 4.1660920931321556E-5 0.0 0.0 3.1245690698491167E-4 1.6664368372528622E-4 22-23 8.332184186264311E-5 0.0 0.0 3.3328736745057245E-4 1.6664368372528622E-4 24-25 8.332184186264311E-5 0.0 0.0 6.665747349011449E-4 1.6664368372528622E-4 26-27 8.332184186264311E-5 4.1660920931321556E-5 0.0 0.0012914885488709681 1.6664368372528622E-4 28-29 8.332184186264311E-5 4.1660920931321556E-5 0.0 0.003374534595437046 1.6664368372528622E-4 30-31 8.332184186264311E-5 6.249138139698234E-5 0.0 0.010290247470036425 1.6664368372528622E-4 32-33 8.332184186264311E-5 8.332184186264311E-5 0.0 0.02516319624251822 1.6664368372528622E-4 34-35 8.332184186264311E-5 1.6664368372528622E-4 0.0 0.04907656485709679 1.6664368372528622E-4 36-37 8.332184186264311E-5 2.0830460465660777E-4 0.0 0.08552987067200316 1.6664368372528622E-4 38-39 8.332184186264311E-5 2.0830460465660777E-4 0.0 0.14312609385955521 1.6664368372528622E-4 40-41 8.332184186264311E-5 2.4996552558792935E-4 0.0 0.20297200677739863 1.6664368372528622E-4 42-43 1.2498276279396467E-4 2.916264465192509E-4 0.0 0.26323452890455523 1.6664368372528622E-4 44-45 1.6664368372528622E-4 2.916264465192509E-4 0.0 0.3395365055902707 1.6664368372528622E-4 46-47 1.6664368372528622E-4 2.916264465192509E-4 0.0 0.426586999876267 1.6664368372528622E-4 48-49 1.6664368372528622E-4 2.916264465192509E-4 0.0 0.5191992271065948 1.6664368372528622E-4 50-51 1.6664368372528622E-4 2.916264465192509E-4 0.0 0.6203727735883093 1.6664368372528622E-4 52-53 1.6664368372528622E-4 2.916264465192509E-4 0.0 0.7112560725999872 2.2913506512226857E-4 54-55 1.6664368372528622E-4 2.916264465192509E-4 0.0 0.8132420070398625 2.4996552558792935E-4 56-57 2.0830460465660777E-4 2.916264465192509E-4 0.0 0.9336628989918474 2.916264465192509E-4 58-59 2.0830460465660777E-4 2.916264465192509E-4 0.0 1.0585415094834838 2.916264465192509E-4 60-61 2.0830460465660777E-4 3.1245690698491167E-4 0.0 1.193377080077706 2.916264465192509E-4 62-63 2.0830460465660777E-4 3.9577874884755477E-4 0.0 1.3274419236346988 2.916264465192509E-4 64-65 2.0830460465660777E-4 4.1660920931321555E-4 0.0 1.4662144512569308 2.916264465192509E-4 66-67 2.916264465192509E-4 4.1660920931321555E-4 0.0 1.6235052582331355 2.916264465192509E-4 68-69 2.916264465192509E-4 4.1660920931321555E-4 0.0 1.794106729446897 2.916264465192509E-4 70-71 3.1245690698491167E-4 4.1660920931321555E-4 0.0 1.9761441234563066 2.916264465192509E-4 72-73 3.3328736745057245E-4 4.1660920931321555E-4 0.0 2.1609103077867178 3.1245690698491167E-4 74-75 3.3328736745057245E-4 4.1660920931321555E-4 0.0 2.3467596760613434 3.3328736745057245E-4 76-77 3.3328736745057245E-4 4.1660920931321555E-4 0.0 2.5418161078617905 3.3328736745057245E-4 78-79 3.3328736745057245E-4 4.1660920931321555E-4 0.0 2.763098089388505 3.3328736745057245E-4 80-81 3.74948288381894E-4 4.1660920931321555E-4 0.0 2.9973366173248603 3.3328736745057245E-4 82-83 3.74948288381894E-4 4.1660920931321555E-4 0.0 3.240740547866107 3.3328736745057245E-4 84-85 4.1660920931321555E-4 4.1660920931321555E-4 0.0 3.489622889509822 3.3328736745057245E-4 86-87 4.1660920931321555E-4 4.1660920931321555E-4 0.0 3.7404841248977743 3.74948288381894E-4 88-89 4.1660920931321555E-4 4.1660920931321555E-4 0.0 4.006780731490782 4.1660920931321555E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4965 0.0 58.851627 1 GTATCAA 9350 0.0 43.915024 1 TGGTATC 2060 0.0 38.29024 2 GTGGTAT 2225 0.0 35.620857 1 ATCAACG 11930 0.0 34.13475 3 TATCAAC 11985 0.0 34.056686 2 TCAACGC 12160 0.0 33.528183 4 CAACGCA 12390 0.0 32.90579 5 AACGCAG 12760 0.0 31.988865 6 ACGCAGA 14300 0.0 28.459059 7 CGCAGAG 14370 0.0 28.25426 8 GCAGAGT 15505 0.0 25.418354 9 GAGTACT 10705 0.0 23.601057 12-13 TAAGGTG 1550 0.0 22.37935 5 CAGAGTA 15045 0.0 22.308619 10-11 GTACTTT 11520 0.0 21.752699 14-15 AGAGTAC 14430 0.0 21.678026 10-11 GTGTAAG 1245 0.0 21.09209 1 GTAAGGT 1630 0.0 20.989458 4 GTACATG 8095 0.0 20.938126 1 >>END_MODULE