FastQCFastQC Report
Thu 26 May 2016
SRR938241_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938241_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2400331
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116020.4833500046451927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102910.4287325373042301No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65210.2716708653931479No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28980.12073334885896987No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27500.11456753256113429No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC27130.11302607848667537No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24720.10298579654222689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47250.061.1299631
GTATCAA89350.043.8643571
TGGTATC18650.034.8974842
TATCAAC116050.033.4448552
ATCAACG115900.033.442973
TCAACGC116450.033.2815484
CAACGCA120250.032.1903235
AACGCAG124750.031.0672256
GTGGTAT21450.031.0065291
ACGCAGA143300.026.946187
CGCAGAG143700.026.8381218
GCAGAGT160400.023.9550469
GTGTAGC16650.022.825911
GAGTACT112550.022.26023712-13
GTACATG79600.021.3659551
TAAGGTG17400.021.2911055
CAGAGTA155800.021.1413110-11
GTACTTT117050.021.12040314-15
AGAGTAC150800.020.85016410-11
TACATGG79550.020.7224772
Produced by FastQC (version 0.11.5)