##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938241_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2400331 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51733115141204 34.0 31.0 34.0 31.0 34.0 2 32.7322273469784 34.0 31.0 34.0 31.0 34.0 3 32.79002020971274 34.0 31.0 34.0 31.0 34.0 4 36.2427352727603 37.0 35.0 37.0 35.0 37.0 5 36.20944403084408 37.0 37.0 37.0 35.0 37.0 6 36.24946559453675 37.0 37.0 37.0 35.0 37.0 7 36.219238096745826 37.0 37.0 37.0 35.0 37.0 8 36.210708023185134 37.0 37.0 37.0 35.0 37.0 9 37.922907715644214 39.0 38.0 39.0 35.0 39.0 10-11 37.90916023665069 39.0 38.0 39.0 35.0 39.0 12-13 37.86108540863739 39.0 38.0 39.0 35.0 39.0 14-15 39.18040928521941 40.5 39.0 41.0 36.0 41.0 16-17 39.13826718065133 40.0 38.5 41.0 36.0 41.0 18-19 39.17619299171656 40.0 39.0 41.0 36.0 41.0 20-21 39.108862277744194 40.0 39.0 41.0 36.0 41.0 22-23 38.97180576345512 40.0 38.5 41.0 35.0 41.0 24-25 38.93515081878291 40.0 38.0 41.0 35.0 41.0 26-27 38.900058991864036 40.0 38.0 41.0 35.0 41.0 28-29 38.83829688488796 40.0 38.0 41.0 35.0 41.0 30-31 38.63076050761333 40.0 38.0 41.0 35.0 41.0 32-33 38.442411484082825 40.0 38.0 41.0 34.0 41.0 34-35 38.23748141402165 40.0 38.0 41.0 34.0 41.0 36-37 38.09773527067725 40.0 38.0 41.0 34.0 41.0 38-39 38.07056985057477 40.0 38.0 41.0 34.0 41.0 40-41 37.9982598233327 40.0 38.0 41.0 33.0 41.0 42-43 37.83523501550411 40.0 38.0 41.0 33.0 41.0 44-45 37.62235000089571 40.0 37.0 41.0 33.0 41.0 46-47 37.291046943109095 40.0 37.0 41.0 32.5 41.0 48-49 36.94007493133239 40.0 37.0 41.0 31.0 41.0 50-51 36.64167587720193 40.0 36.0 41.0 31.0 41.0 52-53 36.2334130167881 39.0 35.5 41.0 30.0 41.0 54-55 36.01433948067996 39.0 35.0 41.0 29.0 41.0 56-57 35.77564719199144 39.0 35.0 40.5 29.0 41.0 58-59 35.45022478149889 38.5 35.0 40.0 28.0 41.0 60-61 35.10850274399656 38.0 35.0 40.0 27.5 41.0 62-63 34.75469154045838 38.0 34.0 40.0 26.5 41.0 64-65 34.38195503036873 37.0 34.0 40.0 26.0 41.0 66-67 33.97900164602298 36.5 34.0 39.5 26.0 41.0 68-69 33.534226737895736 36.0 33.0 39.0 25.0 40.5 70-71 33.03203787311 35.5 33.0 39.0 23.5 40.0 72-73 32.62325050170164 35.0 32.0 38.0 22.5 40.0 74-75 32.17418118584479 35.0 32.0 37.0 21.5 39.0 76-77 30.874582297191512 34.0 30.5 36.0 20.5 38.5 78-79 31.587629789391546 35.0 32.0 36.0 21.0 39.0 80-81 31.418444581184843 35.0 32.0 36.0 20.0 37.5 82-83 31.045759522332546 35.0 31.0 35.5 19.5 37.0 84-85 30.65984170516483 34.0 31.0 35.0 17.5 37.0 86-87 30.230034941014388 34.0 31.0 35.0 12.0 36.0 88-89 29.775073521110215 34.0 30.0 35.0 7.0 36.0 90-91 29.436611034061553 34.0 30.0 35.0 2.0 35.5 92-93 29.044006222475147 34.0 29.0 35.0 2.0 35.0 94-95 28.63981967486984 34.0 29.0 35.0 2.0 35.0 96-97 28.07622615380962 34.0 29.0 35.0 2.0 35.0 98-99 27.41171238466695 34.0 27.5 35.0 2.0 35.0 100-101 25.0064595257904 31.0 22.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14356099889854335 1101 2 0.18983160458711268 1101 3 0.15674383992362806 1101 4 0.016179690263300017 1101 5 0.1043641353977165 1101 6 0.052104632723569466 1101 7 0.05167020945297196 1101 8 0.07180783690985493 1101 9 0.1668431019787704 1101 10-11 0.07375082253172849 1101 12-13 0.1412992887507869 1101 14-15 0.12740499774339042 1101 16-17 0.15883689084288477 1101 18-19 0.1830827300041875 1101 20-21 0.15938463101055333 1101 22-23 0.1955729595754292 1101 24-25 0.14441789397851323 1101 26-27 0.18525025719651467 1101 28-29 0.16418546539344447 1101 30-31 0.18439905320789052 1101 32-33 0.1986119180663124 1101 34-35 0.22926314455052932 1101 36-37 0.28145121812662666 1101 38-39 0.29181497704189496 1101 40-41 0.37913059507473434 1101 42-43 0.33641860831878745 1101 44-45 0.33520398776348515 1101 46-47 0.3182551365629678 1101 48-49 0.3662718900390942 1101 50-51 0.34968895337780737 1101 52-53 0.3988512506658495 1101 54-55 0.3838867351921422 1101 56-57 0.36602070539918685 1101 58-59 0.24069429037070478 1101 60-61 0.38411908544140516 1101 62-63 0.5406686820179516 1101 64-65 0.5232613062313263 1101 66-67 0.2739220951534591 1101 68-69 0.2844313231375679 1101 70-71 0.2708846196820005 1101 72-73 0.2231598971626525 1101 74-75 0.4601116919419681 1101 76-77 0.38154179262130583 1101 78-79 0.3603323525946074 1101 80-81 0.41096787020696723 1101 82-83 0.4885415466589045 1101 84-85 0.44594778438938576 1101 86-87 0.4952542808506415 1101 88-89 0.4579701561541434 1101 90-91 0.44273797899340295 1101 92-93 0.4336977048763906 1101 94-95 0.5367247956743206 1101 96-97 0.6999376467813221 1101 98-99 0.5690167593720297 1101 100-101 0.8684712938496482 1102 1 0.12890054302972942 1102 2 0.12232527485048195 1102 3 0.10541573989664954 1102 4 0.08563546601354233 1102 5 -0.0039025251356648027 1102 6 0.05307552243036895 1102 7 0.049396423666721034 1102 8 0.07174309673199986 1102 9 0.08101125564640199 1102 10-11 0.037389097010070316 1102 12-13 0.12661785621250488 1102 14-15 0.19063421651897983 1102 16-17 0.22534720146156673 1102 18-19 0.12249071515142873 1102 20-21 0.18958720174931898 1102 22-23 0.1268860799178384 1102 24-25 0.07477684206136104 1102 26-27 -0.023791304932863966 1102 28-29 0.05171122570187947 1102 30-31 0.059971412814874725 1102 32-33 0.11856409551914027 1102 34-35 0.16445024047013135 1102 36-37 0.12170546813829475 1102 38-39 0.10136460049470486 1102 40-41 0.17521064119359409 1102 42-43 0.2826271871164252 1102 44-45 0.3629349318435189 1102 46-47 0.3456260698842044 1102 48-49 0.47298457334270694 1102 50-51 0.3542707052086911 1102 52-53 0.4572574326713621 1102 54-55 0.32124571500619226 1102 56-57 0.49767709775702684 1102 58-59 0.3788671862563646 1102 60-61 0.37683038492371423 1102 62-63 0.35939863306032294 1102 64-65 0.3544491628480202 1102 66-67 0.27706422987138524 1102 68-69 0.5050196367262103 1102 70-71 0.6259733147904782 1102 72-73 0.4465585520576809 1102 74-75 0.42317529086123074 1102 76-77 0.4296976239331052 1102 78-79 0.33830354875244595 1102 80-81 0.43180260109759416 1102 82-83 0.6805111877747123 1102 84-85 0.666251049530441 1102 86-87 0.5773457847032155 1102 88-89 0.384768500935202 1102 90-91 0.42807635183847736 1102 92-93 0.5532334155797969 1102 94-95 0.3519135228760355 1102 96-97 0.31730030230415807 1102 98-99 0.16735389512600918 1102 100-101 0.27775113816656116 1103 1 0.1358438569108884 1103 2 0.1788590364224163 1103 3 0.10682668287550712 1103 4 0.07107572220310487 1103 5 0.06638250377637434 1103 6 0.055998365556241936 1103 7 0.0675496692758486 1103 8 0.06957241522606239 1103 9 0.03203904262227297 1103 10-11 0.029578943995453244 1103 12-13 0.040764330933591 1103 14-15 0.1423933798154522 1103 16-17 0.15103801573129516 1103 18-19 0.08919512300691679 1103 20-21 0.14376821078447222 1103 22-23 0.14541786371223253 1103 24-25 0.16948818135342236 1103 26-27 0.14484494802852765 1103 28-29 0.21823792436767064 1103 30-31 0.22513160603829618 1103 32-33 0.2582008626863157 1103 34-35 0.19439909225128815 1103 36-37 0.09943642609075454 1103 38-39 0.07346151364225761 1103 40-41 0.2676737892674552 1103 42-43 0.22796072359277986 1103 44-45 0.23514947135493713 1103 46-47 0.23532360589447876 1103 48-49 0.2863521420094486 1103 50-51 0.32219550706744826 1103 52-53 0.3684169677277609 1103 54-55 0.30832166162299046 1103 56-57 0.46297506782169506 1103 58-59 0.3745157889620572 1103 60-61 0.2859867495564359 1103 62-63 0.41955118691830506 1103 64-65 0.37368211772507465 1103 66-67 0.3913909530699655 1103 68-69 0.4443698739346047 1103 70-71 0.31943253945990335 1103 72-73 0.2421255084693783 1103 74-75 0.2761007654264773 1103 76-77 0.23996705670331409 1103 78-79 0.27721401817460745 1103 80-81 0.43379194878527727 1103 82-83 0.3824351488015587 1103 84-85 0.5487424348941907 1103 86-87 0.5037812860763786 1103 88-89 0.42917634413991124 1103 90-91 0.4700935784488216 1103 92-93 0.47773578172502695 1103 94-95 0.4897600921446319 1103 96-97 0.652710629710505 1103 98-99 0.697369710383807 1103 100-101 0.713746341369994 1104 1 0.15156685809649417 1104 2 0.09539996844780063 1104 3 0.10714641912588263 1104 4 0.049530551454694205 1104 5 0.09005185715319897 1104 6 0.08504557467146157 1104 7 0.06577492095726001 1104 8 0.09193158278559821 1104 9 0.056038160165648776 1104 10-11 0.14634824252728862 1104 12-13 0.1423665026716776 1104 14-15 0.15592055662536097 1104 16-17 0.10959273459867092 1104 18-19 0.2046694704402796 1104 20-21 0.17889339774725244 1104 22-23 0.13948962965140765 1104 24-25 0.15529559445188568 1104 26-27 0.15524312572474486 1104 28-29 0.12394629636632715 1104 30-31 0.028705748207919157 1104 32-33 0.015672720882328406 1104 34-35 0.17478680234577126 1104 36-37 0.142679114775639 1104 38-39 0.09544530930109829 1104 40-41 0.05875594412778895 1104 42-43 0.010872537326555687 1104 44-45 0.09504906877267416 1104 46-47 0.037104583091768006 1104 48-49 0.07399886777911036 1104 50-51 0.032351896277099 1104 52-53 0.15145846941592112 1104 54-55 0.11633691716900074 1104 56-57 0.0958233585633792 1104 58-59 0.07520543961259563 1104 60-61 -0.03656705599919263 1104 62-63 -0.06944336792320627 1104 64-65 0.04899496942531556 1104 66-67 0.021540191604010772 1104 68-69 0.22479659647989791 1104 70-71 0.1926493059814689 1104 72-73 0.018379364395990194 1104 74-75 0.009534937170741387 1104 76-77 -0.003565805163688651 1104 78-79 0.07693416217548688 1104 80-81 0.06530276620700803 1104 82-83 0.19037172377450062 1104 84-85 0.22190034526159153 1104 86-87 0.12525483436195017 1104 88-89 0.18221904532112632 1104 90-91 0.10559955624392714 1104 92-93 0.3699224729832302 1104 94-95 0.14127756655994617 1104 96-97 0.26402252413043215 1104 98-99 0.3276237599263432 1104 100-101 0.5409471218504223 1105 1 0.1266013356979201 1105 2 0.06607171723514682 1105 3 0.0669719243539646 1105 4 0.07161383265817989 1105 5 0.05682537202782356 1105 6 0.08499775901985629 1105 7 0.1054673045351393 1105 8 0.07164192779170264 1105 9 0.010119315909491888 1105 10-11 0.04699594687269126 1105 12-13 0.0744274723011884 1105 14-15 0.1353571300622889 1105 16-17 0.13593466468284277 1105 18-19 0.09910293765743461 1105 20-21 0.1715483024826625 1105 22-23 0.20548709290525835 1105 24-25 0.13081469986058636 1105 26-27 0.16965325315165813 1105 28-29 -3.317951492363136E-4 1105 30-31 0.1754958439686476 1105 32-33 0.15603592569581082 1105 34-35 0.21792766509831552 1105 36-37 0.23298023114527666 1105 38-39 0.21263697744177534 1105 40-41 0.35580025377763747 1105 42-43 0.2284661086020705 1105 44-45 0.24268906077703178 1105 46-47 0.1873940295662777 1105 48-49 0.30992524749434125 1105 50-51 0.20667877413795566 1105 52-53 0.15193033439890513 1105 54-55 0.35439648612348407 1105 56-57 0.295997292490064 1105 58-59 0.32431383949237613 1105 60-61 0.31645572006969047 1105 62-63 0.2862134034201915 1105 64-65 0.17821191442940432 1105 66-67 0.3311645115791606 1105 68-69 0.3566579627276596 1105 70-71 0.2833766287795427 1105 72-73 0.2167869953425665 1105 74-75 0.21613324543141488 1105 76-77 0.29686442784593936 1105 78-79 0.3495279563510323 1105 80-81 0.3882962345170924 1105 82-83 0.40147512925554096 1105 84-85 0.4906036351222305 1105 86-87 0.49262032857445703 1105 88-89 0.40213796723277184 1105 90-91 0.4060754478080959 1105 92-93 0.5208741482567163 1105 94-95 0.38518035194748634 1105 96-97 0.5880222782213664 1105 98-99 0.7360925589553595 1105 100-101 0.866608200197625 1106 1 -0.13941590447065266 1106 2 -0.03781916859707479 1106 3 -0.0640471993716929 1106 4 -0.06615911448079714 1106 5 -0.021027801843693794 1106 6 -0.09977607749015505 1106 7 -0.003668656461222497 1106 8 -0.12214824913812805 1106 9 -0.15223534837629416 1106 10-11 -0.10125141104501978 1106 12-13 -0.13174557903015227 1106 14-15 -0.2352968949210421 1106 16-17 -0.2161559453094597 1106 18-19 -0.1680271405525673 1106 20-21 -0.019773792005146618 1106 22-23 -0.016364625317372372 1106 24-25 -0.01332636524683295 1106 26-27 0.009726630585710438 1106 28-29 0.0263315206653445 1106 30-31 -0.01338238276873227 1106 32-33 0.12857196839946283 1106 34-35 0.030591654773168386 1106 36-37 -0.10220644856042327 1106 38-39 -0.07425358633504686 1106 40-41 -0.013078920791954829 1106 42-43 -0.03240222332046727 1106 44-45 0.027503560672734295 1106 46-47 0.0990994806738712 1106 48-49 0.15709110810005456 1106 50-51 0.23996688365200214 1106 52-53 0.05263643480616054 1106 54-55 -0.2822580881874259 1106 56-57 -0.10783207072927325 1106 58-59 0.004614876538433066 1106 60-61 -0.013304360765872048 1106 62-63 0.14524619253409554 1106 64-65 -0.05026736488603234 1106 66-67 -0.09951756093553854 1106 68-69 -0.22378592370267114 1106 70-71 -0.27673089482890845 1106 72-73 0.01682786628276034 1106 74-75 -0.14763662920912424 1106 76-77 -0.03986187384074924 1106 78-79 -0.025987081491340547 1106 80-81 -0.11884251601173901 1106 82-83 -0.16931366778279155 1106 84-85 -0.013734080954979078 1106 86-87 0.11753985796538657 1106 88-89 0.008287478424627182 1106 90-91 -0.020748878409460048 1106 92-93 0.0040531532888721244 1106 94-95 -0.07424084081979387 1106 96-97 -0.05740908402453826 1106 98-99 0.1283113234733655 1106 100-101 0.2992593595451716 1107 1 0.08890511343372509 1107 2 0.049724517218123765 1107 3 0.007464804355457488 1107 4 0.08278097771567872 1107 5 0.04308382810675937 1107 6 0.03838724009658989 1107 7 0.028597789514378746 1107 8 0.024660977412224838 1107 9 -0.02169722142760122 1107 10-11 0.030398356472780108 1107 12-13 0.07339947513163025 1107 14-15 0.1345781931806158 1107 16-17 0.17537763419972663 1107 18-19 0.12037498065674868 1107 20-21 0.12188754321122985 1107 22-23 0.08454438142680232 1107 24-25 0.1539257654528967 1107 26-27 0.027662453418734856 1107 28-29 0.0882638373508371 1107 30-31 0.09352707725933129 1107 32-33 0.03009455096283631 1107 34-35 0.18405280575586147 1107 36-37 0.15047044829658773 1107 38-39 0.186666540133146 1107 40-41 0.13671066389913022 1107 42-43 0.2646619837114699 1107 44-45 0.18445800066471918 1107 46-47 0.15464260170929833 1107 48-49 0.16376543126026633 1107 50-51 0.21376319260711796 1107 52-53 0.25324483403046827 1107 54-55 0.2835808984742201 1107 56-57 0.2109124457303082 1107 58-59 0.2921995271381874 1107 60-61 0.2609272467261192 1107 62-63 0.3649734822150137 1107 64-65 0.31632199575141584 1107 66-67 0.27597454741191996 1107 68-69 0.47110681791490094 1107 70-71 0.42385278283646954 1107 72-73 0.46029613176508377 1107 74-75 0.4273076062542671 1107 76-77 0.3454250322943082 1107 78-79 0.3362532757733483 1107 80-81 0.422907800225115 1107 82-83 0.5315757110390926 1107 84-85 0.5498312689098306 1107 86-87 0.5070367294438078 1107 88-89 0.5527522716063906 1107 90-91 0.5655924105095771 1107 92-93 0.607552282559066 1107 94-95 0.6856328502649127 1107 96-97 0.7801200840966622 1107 98-99 0.8105891845043587 1107 100-101 0.9109306232050791 1108 1 0.00329253111515726 1108 2 -0.009530555334052337 1108 3 0.03804118048300609 1108 4 -0.010437255767861586 1108 5 -0.0021221239927982083 1108 6 0.02405345180304863 1108 7 0.045060867841243635 1108 8 0.028863005162257593 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-0.4399421201933116 2316 96-97 -0.17743002750559356 2316 98-99 -0.22068461303601694 2316 100-101 -0.2868017720857452 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 10.0 4 21.0 5 43.0 6 69.0 7 168.0 8 520.0 9 1001.0 10 1651.0 11 2753.0 12 4416.0 13 6430.0 14 9086.0 15 12394.0 16 15703.0 17 21739.0 18 21677.0 19 15227.0 20 12603.0 21 11788.0 22 12045.0 23 12869.0 24 14931.0 25 17130.0 26 20530.0 27 24861.0 28 30552.0 29 37140.0 30 45726.0 31 56345.0 32 70993.0 33 92503.0 34 130229.0 35 199763.0 36 332654.0 37 561702.0 38 535707.0 39 67341.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.08085009942379 19.244720832251886 15.22331711751421 22.45111195081012 2 15.876843306595465 20.116943274636693 40.276220464698056 23.729992954069782 3 19.696313810451322 24.1411147103857 29.964856729196832 26.19771474996615 4 13.269503247677092 17.379561402156618 37.176872689641556 32.17406266052474 5 13.778313815183745 37.34644819670629 34.3206559098124 14.554582078297567 6 31.806113406859303 36.63873857397167 17.159341774113653 14.39580624505537 7 28.164948917461803 33.03748524682638 20.429057492487495 18.36850834322433 8 26.22642869313838 35.029660591512055 19.470962866247586 19.272947849101975 9 25.953965362161675 17.08703370338075 20.571773048877173 36.3872278855804 10-11 24.944538899010176 26.816322415533524 27.060892851860846 21.17824583359545 12-13 25.890845887504682 24.188184879502035 27.57121830280907 22.34975093018421 14-15 22.510707898202373 25.67158446064314 26.344762451511894 25.472945189642594 16-17 21.708780390663602 28.793234752279485 27.275774207336838 22.22221064972008 18-19 21.586876976550318 26.92084966615021 30.098598901568156 21.39367445573131 20-21 22.14161296921133 26.023452598829078 30.611944769283905 21.22298966267569 22-23 21.84394568915704 26.091297408565733 30.406243972185504 21.658512930091725 24-25 21.667858235028945 26.256021378236294 30.499067160292608 21.577053226442153 26-27 21.235742213299364 26.651419746990307 30.289303946239503 21.823534093470826 28-29 21.422637720646744 26.979061212416628 30.13648123383035 21.461819833106283 30-31 21.56100554465197 26.521967178693274 30.09072498751214 21.82630228914262 32-33 21.26860168644181 26.56962405012665 30.058075589921344 22.1036986735102 34-35 21.986667158361296 26.74100006104514 29.817508370788588 21.454824409804974 36-37 22.028455005956683 26.23098345565068 29.873819157248704 21.86674238114393 38-39 21.50109838092984 26.718595305796583 29.761081313523587 22.01922499974999 40-41 22.0902986447474 26.483457943126933 29.769702267768377 21.656541144357288 42-43 21.743202671184193 26.51754027066822 29.63490788430979 22.104349173837797 44-45 21.823634620648452 26.81959965287195 29.674115421326043 21.682650305153555 46-47 22.115804028694374 27.072287113735566 28.928781072277115 21.883127785292945 48-49 21.842133439096525 27.181542878877956 28.83596054044213 22.140363141583393 50-51 22.096035921712463 27.373537232989953 28.762012405789033 21.76841443950855 52-53 22.444946134512282 27.105095088968977 28.607575371896626 21.84238340462211 54-55 22.008610479138085 27.309525227978977 28.776656219496395 21.905208073386547 56-57 22.058666075637067 27.45579672136885 28.51452570499652 21.971011497997566 58-59 21.992071093528352 27.19102073838983 28.474822847348968 22.34208532073285 60-61 21.750958513638327 27.39157641175321 28.754180152653948 22.103284921954515 62-63 21.828635300714776 27.320315406500185 28.90940874404405 21.94164054874099 64-65 21.592563692257443 27.39186803819973 28.717247746248333 22.298320523294496 66-67 21.50842946243664 27.488292239695276 28.587973908598443 22.415304389269647 68-69 22.044287798827035 27.116689528177986 28.697122990764782 22.1418996822302 70-71 21.830489211696218 27.324981429644495 28.326489138373002 22.518040220286288 72-73 21.86294306910172 27.290611169876154 28.382043976434918 22.46440178458721 74-75 21.88114889154871 27.70655380445447 28.140598109177446 22.27169919481938 76-77 21.8813363656929 27.35316504265453 28.374628332509143 22.390870259143426 78-79 21.9392658762479 27.609629671907747 28.225107287286626 22.22599716455772 80-81 22.094015367047295 27.68130728637009 27.96510147975425 22.259575866828367 82-83 22.06324173410359 27.70602952636294 28.074312297540814 22.15641644199265 84-85 22.074111631561603 27.7650940247997 28.03643838549897 22.124355958139727 86-87 22.11549337494446 27.741369430194606 27.801632241041247 22.341504953819687 88-89 22.006045837883057 27.94816877186419 27.626816990504217 22.418968399748536 90-91 21.958051921729748 27.873168815299763 27.794950224438402 22.37382903853208 92-93 22.028753171703933 27.545356850411807 27.9950873349938 22.430802642890455 94-95 21.823656820663484 27.467878388438926 28.067837310770887 22.6406274801267 96-97 22.1401756674392 27.149089021472456 28.066004230249913 22.644731080838433 98-99 21.822157027509956 27.45356786209902 27.731008765041153 22.99326634534987 100-101 21.967720285243995 27.21980843475337 27.999409248141195 22.81306203186144 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1031.0 1 675.0 2 509.0 3 729.0 4 558.0 5 478.0 6 1033.0 7 2412.5 8 3856.0 9 4480.0 10 3710.5 11 2618.5 12 2469.5 13 2610.5 14 2775.0 15 3000.5 16 3273.5 17 3501.0 18 3772.5 19 4163.5 20 4792.5 21 5738.0 22 6852.0 23 8321.5 24 10268.0 25 12882.0 26 16570.5 27 21208.0 28 24828.5 29 28187.5 30 32379.0 31 37488.5 32 44762.0 33 48635.5 34 50003.0 35 52746.5 36 56820.0 37 61686.0 38 65095.0 39 67779.5 40 71173.5 41 75558.5 42 78000.5 43 79355.0 44 81452.5 45 84864.0 46 86017.0 47 86395.5 48 86737.5 49 84703.5 50 83817.0 51 82239.5 52 79958.0 53 79373.5 54 79314.5 55 74108.5 56 67244.0 57 61304.5 58 56454.5 59 49797.5 60 38955.0 61 31954.0 62 27028.5 63 19475.5 64 13213.0 65 10100.5 66 7472.5 67 5529.5 68 4126.0 69 3028.0 70 2208.0 71 1457.0 72 1053.5 73 867.0 74 635.0 75 436.5 76 303.0 77 186.5 78 110.0 79 61.5 80 30.5 81 17.0 82 12.5 83 6.5 84 2.5 85 2.0 86 1.0 87 1.5 88 1.5 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015164575219001044 3 0.006499103665286163 4 0.0 5 4.1660920931321556E-5 6 0.0 7 0.0 8 3.3328736745057245E-4 9 3.3328736745057245E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.0830460465660777E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005540902483865767 26-27 0.020913782307523422 28-29 4.1660920931321556E-5 30-31 0.0 32-33 4.582701302445371E-4 34-35 0.019476480535392825 36-37 0.003562008739627993 38-39 0.018122500605124877 40-41 0.01551869304691728 42-43 0.008207201423470346 44-45 0.0026662989396045796 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.541178279162332E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.249138139698234E-5 84-85 0.0024996552558792934 86-87 4.791005907101979E-4 88-89 8.332184186264311E-5 90-91 2.707959860535901E-4 92-93 1.87474144190947E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2400331.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.9123664790088 #Duplication Level Percentage of deduplicated Percentage of total 1 77.28349318160068 44.756699799108354 2 13.635532122506241 15.793318668297562 3 4.175262918112853 7.253980687799018 4 1.7170812912194935 3.9776096404541175 5 0.8946501278084166 2.5905653036066543 6 0.5361509482887986 1.8629862123221401 7 0.3317580258807102 1.3449024664009321 8 0.23208106263744388 1.0752290841837957 9 0.1643531226920923 0.8566270445982552 >10 0.924551349738729 9.760088819327391 >50 0.0662054718333084 2.629807258895366 >100 0.033558707274226926 3.6233418592327578 >500 0.002948493063377283 1.2315543338925476 >1k 0.0021574339488114685 2.0599204933426383 >5k 7.191446496038228E-5 0.271582489983772 >10k+ 1.4382892992076456E-4 0.9117858385546266 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11602 0.4833500046451927 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10291 0.4287325373042301 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6521 0.2716708653931479 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2898 0.12073334885896987 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2750 0.11456753256113429 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2713 0.11302607848667537 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2472 0.10298579654222689 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.1660920931321556E-5 2 4.1660920931321556E-5 0.0 0.0 0.0 4.1660920931321556E-5 3 4.1660920931321556E-5 0.0 0.0 0.0 4.1660920931321556E-5 4 4.1660920931321556E-5 0.0 0.0 0.0 4.1660920931321556E-5 5 4.1660920931321556E-5 0.0 0.0 0.0 4.1660920931321556E-5 6 4.1660920931321556E-5 0.0 0.0 0.0 4.1660920931321556E-5 7 4.1660920931321556E-5 0.0 0.0 4.1660920931321556E-5 4.1660920931321556E-5 8 4.1660920931321556E-5 0.0 0.0 4.1660920931321556E-5 4.1660920931321556E-5 9 4.1660920931321556E-5 0.0 0.0 4.1660920931321556E-5 4.1660920931321556E-5 10-11 8.332184186264311E-5 0.0 0.0 8.332184186264311E-5 4.1660920931321556E-5 12-13 1.2498276279396467E-4 0.0 0.0 8.332184186264311E-5 2.2913506512226857E-4 14-15 1.2498276279396467E-4 0.0 0.0 8.332184186264311E-5 2.916264465192509E-4 16-17 1.2498276279396467E-4 0.0 0.0 1.041523023283039E-4 4.1660920931321555E-4 18-19 1.87474144190947E-4 0.0 0.0 2.0830460465660777E-4 4.1660920931321555E-4 20-21 2.0830460465660777E-4 0.0 0.0 3.541178279162332E-4 4.1660920931321555E-4 22-23 2.0830460465660777E-4 0.0 0.0 4.1660920931321555E-4 4.1660920931321555E-4 24-25 2.0830460465660777E-4 0.0 0.0 7.082356558324664E-4 4.1660920931321555E-4 26-27 2.0830460465660777E-4 0.0 0.0 0.0013331494698022898 4.1660920931321555E-4 28-29 2.0830460465660777E-4 0.0 0.0 0.0034370259768340284 4.1660920931321555E-4 30-31 2.0830460465660777E-4 4.1660920931321556E-5 0.0 0.010373569311899068 4.1660920931321555E-4 32-33 2.2913506512226857E-4 8.332184186264311E-5 0.0 0.025350670386709163 4.1660920931321555E-4 34-35 2.4996552558792935E-4 1.6664368372528622E-4 0.0 0.04936819130361604 4.1660920931321555E-4 36-37 2.4996552558792935E-4 2.0830460465660777E-4 0.0 0.08588398849991938 4.1660920931321555E-4 38-39 2.4996552558792935E-4 2.0830460465660777E-4 0.0 0.14389682089678463 4.1660920931321555E-4 40-41 2.4996552558792935E-4 2.2913506512226857E-4 0.0 0.20438847808906355 4.1660920931321555E-4 42-43 2.707959860535901E-4 2.4996552558792935E-4 0.0 0.26494262666273943 4.1660920931321555E-4 44-45 2.916264465192509E-4 2.4996552558792935E-4 0.0 0.3417028734786994 4.5827013024453715E-4 46-47 3.1245690698491167E-4 2.4996552558792935E-4 0.0 0.4292532988158716 4.999310511758587E-4 48-49 3.3328736745057245E-4 2.4996552558792935E-4 0.0 0.5223029657159783 4.999310511758587E-4 50-51 3.3328736745057245E-4 2.707959860535901E-4 0.0 0.6237889691046776 4.999310511758587E-4 52-53 3.3328736745057245E-4 2.916264465192509E-4 0.0 0.715442995153585 4.999310511758587E-4 54-55 3.3328736745057245E-4 2.916264465192509E-4 0.0 0.817387268672529 4.999310511758587E-4 56-57 3.3328736745057245E-4 2.916264465192509E-4 0.0 0.9376415169407886 4.999310511758587E-4 58-59 3.3328736745057245E-4 2.916264465192509E-4 0.0 1.0632491935487232 4.999310511758587E-4 60-61 3.3328736745057245E-4 2.916264465192509E-4 0.0 1.1992929308499536 4.999310511758587E-4 62-63 3.3328736745057245E-4 2.916264465192509E-4 0.0 1.334545110653489 4.999310511758587E-4 64-65 4.582701302445371E-4 3.1245690698491167E-4 0.0 1.4734217905780493 4.999310511758587E-4 66-67 4.582701302445371E-4 3.3328736745057245E-4 0.0 1.6316499682752088 4.999310511758587E-4 68-69 4.582701302445371E-4 3.3328736745057245E-4 0.0 1.8035637584983073 4.999310511758587E-4 70-71 4.582701302445371E-4 3.3328736745057245E-4 0.0 1.9864968623077401 4.999310511758587E-4 72-73 4.582701302445371E-4 3.3328736745057245E-4 0.0 2.1722212478195715 5.207615116415195E-4 74-75 4.582701302445371E-4 3.3328736745057245E-4 0.0 2.360112001219832 5.415919721071802E-4 76-77 4.582701302445371E-4 3.3328736745057245E-4 0.0 2.5577305796575556 5.415919721071802E-4 78-79 4.582701302445371E-4 3.3328736745057245E-4 0.0 2.7816371992029434 5.415919721071802E-4 80-81 4.999310511758587E-4 3.3328736745057245E-4 0.0 3.0182920605533154 5.415919721071802E-4 82-83 4.999310511758587E-4 3.3328736745057245E-4 0.0 3.264799729703945 5.832528930385018E-4 84-85 4.999310511758587E-4 3.3328736745057245E-4 0.0 3.5165983358128523 5.832528930385018E-4 86-87 4.999310511758587E-4 3.3328736745057245E-4 0.0 3.772250577107907 5.832528930385018E-4 88-89 4.999310511758587E-4 3.3328736745057245E-4 0.0 4.044775491380147 5.832528930385018E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4725 0.0 61.129963 1 GTATCAA 8935 0.0 43.864357 1 TGGTATC 1865 0.0 34.897484 2 TATCAAC 11605 0.0 33.444855 2 ATCAACG 11590 0.0 33.44297 3 TCAACGC 11645 0.0 33.281548 4 CAACGCA 12025 0.0 32.190323 5 AACGCAG 12475 0.0 31.067225 6 GTGGTAT 2145 0.0 31.006529 1 ACGCAGA 14330 0.0 26.94618 7 CGCAGAG 14370 0.0 26.838121 8 GCAGAGT 16040 0.0 23.955046 9 GTGTAGC 1665 0.0 22.82591 1 GAGTACT 11255 0.0 22.260237 12-13 GTACATG 7960 0.0 21.365955 1 TAAGGTG 1740 0.0 21.291105 5 CAGAGTA 15580 0.0 21.14131 10-11 GTACTTT 11705 0.0 21.120403 14-15 AGAGTAC 15080 0.0 20.850164 10-11 TACATGG 7955 0.0 20.722477 2 >>END_MODULE