FastQCFastQC Report
Thu 26 May 2016
SRR938240_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938240_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2438636
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120020.49216037161757636No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98610.40436539114488596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59400.2435787874861193No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27160.11137373515358587No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26210.10747811481500315No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26070.10690402339668569No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC25400.10415658589473789No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24530.1005890177951937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49900.061.654761
GTATCAA93500.044.365071
TGGTATC17500.040.494232
GTGGTAT20950.035.6903041
ATCAACG119600.034.1598433
TATCAAC119800.034.1418152
TCAACGC121150.033.7227974
CAACGCA123600.033.0543445
AACGCAG127450.032.2737776
ACGCAGA142800.028.5317177
CGCAGAG143100.028.3699058
GCAGAGT156600.025.3160579
GAGTACT112500.023.5778112-13
GTACTTT115400.022.69485514-15
TACGTTG1050.002180512322.6482475
CAGAGTA151800.022.59318210-11
AGAGTAC147650.021.40923110-11
GTACATG80050.020.5849291
TAAGGTA10100.020.2488784
AGTACTT117800.019.99494212-13
Produced by FastQC (version 0.11.5)