##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938240_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2438636 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.978726222363647 33.0 31.0 34.0 30.0 34.0 2 32.10335818875798 34.0 31.0 34.0 30.0 34.0 3 32.12575021446415 34.0 31.0 34.0 30.0 34.0 4 35.63367636662462 37.0 35.0 37.0 35.0 37.0 5 35.64195804539915 37.0 35.0 37.0 35.0 37.0 6 35.67554608395841 37.0 35.0 37.0 35.0 37.0 7 35.61431185301947 37.0 35.0 37.0 33.0 37.0 8 35.601233230379606 37.0 35.0 37.0 33.0 37.0 9 37.241912282111805 39.0 37.0 39.0 34.0 39.0 10-11 37.1446017363805 39.0 37.0 39.0 34.0 39.0 12-13 36.998674873986936 39.0 37.0 39.0 33.0 39.0 14-15 38.17050699653413 40.0 38.0 41.0 33.0 41.0 16-17 37.98668189922563 40.0 38.0 41.0 33.0 41.0 18-19 38.181921984256775 40.0 38.0 41.0 33.5 41.0 20-21 38.18668960845325 40.0 38.0 41.0 33.5 41.0 22-23 38.067942079096674 40.0 38.0 41.0 33.0 41.0 24-25 37.947398668764016 40.0 38.0 41.0 33.0 41.0 26-27 37.75900298363511 40.0 38.0 41.0 32.5 41.0 28-29 37.56962724244208 40.0 37.5 41.0 32.0 41.0 30-31 37.321943701315 40.0 37.0 41.0 31.5 41.0 32-33 37.0047083697608 40.0 36.5 41.0 31.0 41.0 34-35 36.751786654506866 39.5 36.0 41.0 30.0 41.0 36-37 36.467109482514 39.0 35.5 41.0 30.0 41.0 38-39 36.144756741063446 39.0 35.0 40.5 29.5 41.0 40-41 35.99479791161944 39.0 35.0 40.5 29.0 41.0 42-43 35.75289608617277 39.0 35.0 40.5 27.5 41.0 44-45 35.39593034794861 39.0 35.0 40.0 26.5 41.0 46-47 34.9818306217082 38.0 34.5 40.0 24.5 41.0 48-49 34.55049585095931 38.0 34.0 40.0 23.5 41.0 50-51 33.80885851763035 37.5 33.5 39.5 22.5 40.5 52-53 33.87427336429053 38.0 33.5 40.0 22.0 40.5 54-55 33.94137788501441 38.0 33.5 40.0 21.0 41.0 56-57 33.609578879340745 38.0 33.0 40.0 18.5 41.0 58-59 33.18905014934578 37.5 33.0 40.0 15.5 41.0 60-61 32.676435925656804 37.0 32.0 40.0 10.5 41.0 62-63 32.12059200307057 36.0 31.0 39.0 7.5 41.0 64-65 31.570279656332474 35.5 31.0 39.0 4.0 40.0 66-67 30.976571329218466 35.0 30.0 39.0 2.0 40.0 68-69 30.271528223154256 35.0 29.0 38.0 2.0 40.0 70-71 29.663546753184978 34.5 28.5 37.5 2.0 39.5 72-73 28.952854177499226 34.0 27.5 37.0 2.0 39.0 74-75 28.25437068098724 34.0 26.0 36.0 2.0 39.0 76-77 27.48749833923554 33.5 25.5 35.5 2.0 38.5 78-79 26.696272219388216 33.0 23.5 35.0 2.0 37.0 80-81 25.950273431541238 32.0 21.0 35.0 2.0 37.0 82-83 25.18884286133724 31.5 18.5 35.0 2.0 36.0 84-85 24.423956670860267 31.0 11.5 34.5 2.0 36.0 86-87 23.654919184330915 31.0 4.5 34.0 2.0 35.0 88-89 22.752840317292126 30.0 2.0 34.0 2.0 35.0 90-91 21.817612181563792 29.0 2.0 34.0 2.0 35.0 92-93 20.8563537157657 29.0 2.0 34.0 2.0 35.0 94-95 19.746714761858676 26.5 2.0 33.5 2.0 35.0 96-97 18.454993898228352 25.0 2.0 33.0 2.0 35.0 98-99 16.878569823458687 11.0 2.0 32.5 2.0 34.0 100-101 13.033370498918249 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2236403405289238 1101 2 0.2972357208292351 1101 3 0.16056921521018097 1101 4 0.09217853934123355 1101 5 0.18996374936163107 1101 6 0.1459549444579764 1101 7 0.24263822771253984 1101 8 0.2875277742753042 1101 9 0.24050131171110678 1101 10-11 0.2894022104891647 1101 12-13 0.27799061974347694 1101 14-15 0.42211301923666866 1101 16-17 0.3874072223117011 1101 18-19 0.46067718571298144 1101 20-21 0.5456783642742309 1101 22-23 0.4569317743632624 1101 24-25 0.3635016948371259 1101 26-27 0.4007886464594037 1101 28-29 0.3460453465087667 1101 30-31 0.35956555352736075 1101 32-33 0.26856859793702625 1101 34-35 0.24912051511533662 1101 36-37 0.09252405642136097 1101 38-39 0.1798187392331272 1101 40-41 0.4351760764513699 1101 42-43 0.49560926397417404 1101 44-45 0.4779508959160168 1101 46-47 0.45433684674842567 1101 48-49 0.550697758538476 1101 50-51 0.4770619494778998 1101 52-53 0.26468602293603993 1101 54-55 0.2488755003903833 1101 56-57 0.25849804718367864 1101 58-59 0.4155006341070049 1101 60-61 0.44673294318928214 1101 62-63 0.17645544144249925 1101 64-65 0.13411731663801874 1101 66-67 0.07339917009198516 1101 68-69 0.11383558134346927 1101 70-71 0.06616877611008931 1101 72-73 -0.002525775190552082 1101 74-75 -0.03483079136781342 1101 76-77 -0.024760748043025416 1101 78-79 -0.16088466462889883 1101 80-81 -0.3096736781762566 1101 82-83 -0.4507565916029712 1101 84-85 -0.4735378425139416 1101 86-87 -0.3286590210837872 1101 88-89 -0.2449519696604021 1101 90-91 -0.17593771922816615 1101 92-93 -0.1473838979123343 1101 94-95 -0.4402313747254105 1101 96-97 -0.5393116434919918 1101 98-99 -0.6956582572618188 1101 100-101 -0.7641257750133104 1102 1 0.13166407278477976 1102 2 0.19426597070475538 1102 3 0.12280056921706262 1102 4 0.13169578274233373 1102 5 0.027919268023659072 1102 6 0.17604170865786273 1102 7 0.15519425038853285 1102 8 0.07734253051535944 1102 9 0.12980295060513214 1102 10-11 0.22398399196892882 1102 12-13 0.16188004878553386 1102 14-15 0.24868061267687125 1102 16-17 0.2565756345266266 1102 18-19 0.1251773740624671 1102 20-21 0.2165678874359287 1102 22-23 0.27248450461528506 1102 24-25 0.1435056359592295 1102 26-27 0.2465812230870128 1102 28-29 0.07667449992356268 1102 30-31 0.09816842574133489 1102 32-33 0.12948666337508996 1102 34-35 0.15614816623080685 1102 36-37 0.08227173864260351 1102 38-39 -0.002165990461662659 1102 40-41 0.08255332361284928 1102 42-43 0.09080246161505556 1102 44-45 0.01447229138183559 1102 46-47 -0.08489473142630288 1102 48-49 -0.12989027539485676 1102 50-51 -0.2599919162713462 1102 52-53 -0.2778493308909802 1102 54-55 -0.2808017221501018 1102 56-57 -0.1349853030081576 1102 58-59 -0.21055157165395144 1102 60-61 -0.1442881314542106 1102 62-63 -0.3536527035652277 1102 64-65 -0.4090840383321037 1102 66-67 -0.41374369051462523 1102 68-69 -0.422553120861334 1102 70-71 -0.7133401001212647 1102 72-73 -0.6199288931295115 1102 74-75 -0.42634710723861247 1102 76-77 -0.6599142346907136 1102 78-79 -0.5317000603094009 1102 80-81 -0.46335760238768486 1102 82-83 -0.4100064250200255 1102 84-85 -0.42370193470357975 1102 86-87 -0.5569333099570173 1102 88-89 -0.7121222902777404 1102 90-91 -0.7462995331935396 1102 92-93 -0.9789478775551608 1102 94-95 -1.0184690406994399 1102 96-97 -0.88820046968344 1102 98-99 -1.0225054543591447 1102 100-101 -1.0733932090232106 1103 1 0.2207586438270681 1103 2 0.22488069064968386 1103 3 0.24299581824509175 1103 4 0.2166204518186987 1103 5 0.29010721240204873 1103 6 0.16446084046517484 1103 7 0.24689580594234428 1103 8 0.2572823947993754 1103 9 0.3681617620748483 1103 10-11 0.3180265283643209 1103 12-13 0.22593568482714943 1103 14-15 0.2201564672468166 1103 16-17 0.19069797414508116 1103 18-19 0.2958963436786348 1103 20-21 0.36651819875677916 1103 22-23 0.4307054398150072 1103 24-25 0.45527923213997923 1103 26-27 0.4371365118490047 1103 28-29 0.3561588369017201 1103 30-31 0.3151146314594655 1103 32-33 0.28753719494739727 1103 34-35 0.36432824063544444 1103 36-37 0.2746537203370494 1103 38-39 0.21716533551835226 1103 40-41 0.3154545504128592 1103 42-43 0.36864854703205197 1103 44-45 0.28158910232568957 1103 46-47 0.3675669007009148 1103 48-49 0.29805647124339174 1103 50-51 0.3366371264910484 1103 52-53 0.3657642149029172 1103 54-55 0.2310903501765793 1103 56-57 0.2320488314061322 1103 58-59 0.34896109943669984 1103 60-61 0.440685239530616 1103 62-63 0.5037891708661846 1103 64-65 0.33802223008098053 1103 66-67 0.2905984825157226 1103 68-69 0.22705946023158674 1103 70-71 0.07085389178212154 1103 72-73 -0.05503344095404472 1103 74-75 0.01992970082769574 1103 76-77 0.0913472932844499 1103 78-79 -0.05376584459792966 1103 80-81 0.16897121252617353 1103 82-83 0.09955485248514506 1103 84-85 -0.057460612184687676 1103 86-87 -0.21644330721740346 1103 88-89 -0.268047996842693 1103 90-91 -0.37801241220632775 1103 92-93 -0.3921494052035186 1103 94-95 -0.40554798935786707 1103 96-97 -0.6573080109246163 1103 98-99 -0.9070899952486933 1103 100-101 -0.9443043973264338 1104 1 0.22042236146436522 1104 2 0.20589862451653573 1104 3 0.22352025973216172 1104 4 0.14054153596759278 1104 5 0.1992591011283622 1104 6 0.2041039995636993 1104 7 0.19194762562344891 1104 8 0.20403331490528132 1104 9 0.21795564006635715 1104 10-11 0.3316735900872416 1104 12-13 0.34883714972241364 1104 14-15 0.3121405252368348 1104 16-17 0.22810869137653356 1104 18-19 0.30696583686613366 1104 20-21 0.19462921804611 1104 22-23 0.37353743815506135 1104 24-25 0.4693548948765951 1104 26-27 0.41607136330242867 1104 28-29 0.22255178721500357 1104 30-31 0.2559753433843355 1104 32-33 0.2596342056515937 1104 34-35 0.29833342319980005 1104 36-37 0.3276074600473535 1104 38-39 0.14713771345997628 1104 40-41 0.2960941666216712 1104 42-43 0.4003365016442686 1104 44-45 0.4584172799257047 1104 46-47 0.22119572924182052 1104 48-49 0.1683166687277904 1104 50-51 0.19964409898386748 1104 52-53 0.21627100355343032 1104 54-55 0.08634464284479293 1104 56-57 0.027650369401072794 1104 58-59 0.15393640891784344 1104 60-61 0.11067925322756622 1104 62-63 0.15404478123656418 1104 64-65 -0.06045666859825616 1104 66-67 -0.12054754869652484 1104 68-69 -0.07601909349985547 1104 70-71 -0.1658474525125868 1104 72-73 -0.13171396833700655 1104 74-75 -0.19906136860984347 1104 76-77 -0.2697942263381812 1104 78-79 -0.45306696557889126 1104 80-81 -0.4143667913955298 1104 82-83 -0.4860385487486063 1104 84-85 -0.2721702426994099 1104 86-87 -0.2827230305436714 1104 88-89 -0.22949914666205018 1104 90-91 -0.3080194717509599 1104 92-93 -0.5892475388364069 1104 94-95 -0.5057416498390843 1104 96-97 -0.45237243125690085 1104 98-99 -0.6352258779680326 1104 100-101 -1.033326507295536 1105 1 0.2072978934924592 1105 2 0.23133621762145395 1105 3 0.21898435079172174 1105 4 0.1996339699369969 1105 5 0.23923103664655798 1105 6 0.21253442802365186 1105 7 0.1651433853595634 1105 8 0.16614330875740535 1105 9 0.3027653513524484 1105 10-11 0.29938299486602915 1105 12-13 0.21121792370505688 1105 14-15 0.20129228772767505 1105 16-17 0.3915822658368171 1105 18-19 0.3067216211937662 1105 20-21 0.3179208167314087 1105 22-23 0.3455028829305391 1105 24-25 0.33456705585647484 1105 26-27 0.2888536559297066 1105 28-29 0.2898601758201451 1105 30-31 0.21697953162769323 1105 32-33 0.12871465421038408 1105 34-35 0.1433658325776861 1105 36-37 0.203912591458284 1105 38-39 0.22901258784224154 1105 40-41 0.2681793177176459 1105 42-43 0.30273169949960277 1105 44-45 0.23392802689314607 1105 46-47 0.39481773953952626 1105 48-49 0.3434821146855569 1105 50-51 0.29747907717072053 1105 52-53 0.29732806947762214 1105 54-55 0.2836345139166454 1105 56-57 0.34699782955807734 1105 58-59 0.2886470299719619 1105 60-61 0.3200311986769506 1105 62-63 0.4972876919622493 1105 64-65 0.49285827281954653 1105 66-67 0.3724673658242956 1105 68-69 0.24356445412257344 1105 70-71 0.041854097462589834 1105 72-73 0.0688874081378934 1105 74-75 -0.01367507188004069 1105 76-77 -0.23257017896403553 1105 78-79 -0.27074962116754975 1105 80-81 -0.22429827800024427 1105 82-83 -0.4817612645746685 1105 84-85 -0.5328231815634723 1105 86-87 -0.4570388941434942 1105 88-89 -0.3855808558318472 1105 90-91 -0.4545294783126401 1105 92-93 -0.7541529708432186 1105 94-95 -1.0810330460581383 1105 96-97 -1.100108503943762 1105 98-99 -1.2675386779727624 1105 100-101 -1.09413676391042 1106 1 0.3799593500338645 1106 2 0.3953687526317111 1106 3 0.392241125069539 1106 4 0.29986815479370676 1106 5 0.2909908277318394 1106 6 0.2818863559770648 1106 7 0.3488395240371389 1106 8 0.11788195511238797 1106 9 0.4823681330591896 1106 10-11 0.4497442272971952 1106 12-13 0.49152228437760925 1106 14-15 0.5144014431171584 1106 16-17 0.06547029592844211 1106 18-19 0.4364688321600312 1106 20-21 0.6679995022061362 1106 22-23 0.539144212555783 1106 24-25 0.5310250945174815 1106 26-27 0.5840144793238196 1106 28-29 0.5537851355217143 1106 30-31 0.6497952371847333 1106 32-33 0.5881745118756925 1106 34-35 0.5602493223262712 1106 36-37 0.5141540674639913 1106 38-39 0.5533505507167078 1106 40-41 0.48352525506234656 1106 42-43 0.2791508453958542 1106 44-45 0.4914604886335283 1106 46-47 0.6200607844762587 1106 48-49 0.6959920459393061 1106 50-51 0.6370408476576088 1106 52-53 0.6117320907195207 1106 54-55 0.618510795343056 1106 56-57 0.34885217462097273 1106 58-59 0.4914777558534311 1106 60-61 0.7664416517396333 1106 62-63 0.47430116008029444 1106 64-65 0.41578145015175494 1106 66-67 0.32996884715003105 1106 68-69 0.16030130456919878 1106 70-71 0.14270092200990803 1106 72-73 0.25319150758741316 1106 74-75 0.1678293418335315 1106 76-77 -0.30866900807957265 1106 78-79 -0.4189282873691127 1106 80-81 -0.44288859745690345 1106 82-83 -0.2627558155259031 1106 84-85 -0.0858262708444073 1106 86-87 0.02000862057419539 1106 88-89 -0.3837249179455924 1106 90-91 -0.4199754648755807 1106 92-93 -0.44807304455908437 1106 94-95 -0.4324308879417451 1106 96-97 -0.6509215606355312 1106 98-99 -0.8251974904273638 1106 100-101 -0.9410852527988833 1107 1 0.08035193056973355 1107 2 -0.04880310947139321 1107 3 -0.036179037616008713 1107 4 0.12977568030797926 1107 5 0.03076520828437168 1107 6 0.082430621569344 1107 7 0.17313308045667242 1107 8 0.1458211287304536 1107 9 0.11521703283679585 1107 10-11 0.15932402342358642 1107 12-13 0.14241064341065623 1107 14-15 0.13047668750723318 1107 16-17 0.1547811376647772 1107 18-19 0.19050138931712723 1107 20-21 0.23496837259805403 1107 22-23 0.2958123499642582 1107 24-25 0.09879685871693056 1107 26-27 0.09678633191357733 1107 28-29 0.08113063609971505 1107 30-31 0.04532327085397014 1107 32-33 0.18000639096067061 1107 34-35 0.09994495349411636 1107 36-37 0.15625706786840254 1107 38-39 0.10098900775364683 1107 40-41 0.026546093122739478 1107 42-43 0.0260137640583622 1107 44-45 -0.07577929349255896 1107 46-47 0.03226898028065506 1107 48-49 -0.1614827760362374 1107 50-51 -0.0019612932103001413 1107 52-53 -0.06684776682447335 1107 54-55 -0.24583969901390645 1107 56-57 -0.28420840627283894 1107 58-59 -0.19678803908433906 1107 60-61 0.030964862578578334 1107 62-63 -0.07499411402716305 1107 64-65 -0.12106136108281618 1107 66-67 -0.26613592871493097 1107 68-69 -0.4046843424852078 1107 70-71 -0.29912868808678184 1107 72-73 -0.3088390783747137 1107 74-75 -0.46271585140134874 1107 76-77 -0.5518748461408798 1107 78-79 -0.699795594929288 1107 80-81 -0.5740453240412613 1107 82-83 -0.604054937983296 1107 84-85 -0.7113882555038593 1107 86-87 -0.6995009608439346 1107 88-89 -0.5702156168249672 1107 90-91 -0.5848475349419395 1107 92-93 -0.7763878131942654 1107 94-95 -0.7485070617969107 1107 96-97 -0.9137084031278313 1107 98-99 -0.8284897104219091 1107 100-101 -0.8447080160912819 1108 1 -0.05098841728212378 1108 2 0.024343905987720404 1108 3 -0.011194201228313716 1108 4 0.017640008578091226 1108 5 1.876378762801778E-4 1108 6 0.030056385542259534 1108 7 0.04509919213339941 1108 8 0.046462863758591766 1108 9 0.06657710929959393 1108 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66-67 -0.6512135406717086 2316 68-69 -0.7104771596225241 2316 70-71 -0.7660105901243668 2316 72-73 -0.6636499464493433 2316 74-75 -0.748515426390977 2316 76-77 -0.8303273749885243 2316 78-79 -0.6632073577774555 2316 80-81 -0.6907046752643318 2316 82-83 -0.750045152705578 2316 84-85 -1.0617262829170642 2316 86-87 -1.0736824987868232 2316 88-89 -1.0348858114226118 2316 90-91 -0.9020785691462514 2316 92-93 -1.0567858563441241 2316 94-95 -0.9272804345750174 2316 96-97 -1.0192807170901546 2316 98-99 -1.2634796590152462 2316 100-101 -1.0247200503062466 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7824.0 3 2393.0 4 2357.0 5 2420.0 6 3098.0 7 3902.0 8 4746.0 9 6249.0 10 7780.0 11 9909.0 12 12579.0 13 15502.0 14 18593.0 15 23089.0 16 26694.0 17 33076.0 18 32349.0 19 27444.0 20 27791.0 21 29529.0 22 32184.0 23 35503.0 24 39671.0 25 44423.0 26 50129.0 27 57119.0 28 64869.0 29 75433.0 30 87453.0 31 104132.0 32 125303.0 33 155553.0 34 196869.0 35 255241.0 36 314909.0 37 328194.0 38 164747.0 39 9580.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.760623529030084 18.779750501325847 14.915342357403954 22.54428361224011 2 15.568032395108734 19.89718573646973 40.8310888014097 23.70369306701184 3 19.564028199069067 24.225310003310096 29.68200071130123 26.528661086319605 4 12.939061198295715 17.07975622344241 37.43936072500656 32.541821853255314 5 13.776262334093703 37.26227492949599 34.218851688480726 14.742611047929582 6 31.753428709950448 36.468613642526684 17.3307010263532 14.447256621169666 7 28.11348537279103 33.04120673190467 20.493125365808577 18.35218252949572 8 26.18447909511041 34.74090378982426 19.649315891458556 19.425301223606773 9 26.146398581430418 17.064835346654935 20.561868839792208 36.22689723212244 10-11 24.89772763656634 26.72615143123841 27.10270994055355 21.273410991641704 12-13 25.919777904632053 24.221413640302426 27.5518995971236 22.30690885794192 14-15 22.626652513750845 25.640320206298593 26.356205896181383 25.376821383769176 16-17 21.73564018387463 28.757429614984265 27.20336460187529 22.303565599265816 18-19 21.623784541083037 26.89101612591294 30.049392357796282 21.43580697520774 20-21 22.17447897953695 26.068535927879395 30.519100832934694 21.237884259648965 22-23 21.883509462188123 26.106596013799415 30.33239429443995 21.677500229572512 24-25 21.667889585152214 26.23206383367377 30.458231768771626 21.641814812402387 26-27 21.236841256898543 26.64263680195546 30.20128536641608 21.919236574729915 28-29 21.416316722490077 26.947053129583875 30.097731171018772 21.538898976907277 30-31 21.595865356454357 26.494671712512396 30.039994737696546 21.8694681933367 32-33 21.22492626534458 26.582577579204063 29.973437663910392 22.219058491540967 34-35 21.96261298645435 26.78625519936758 29.74339935822134 21.50773245595673 36-37 22.139519669428743 26.21693040802837 29.761570804464338 21.881979118078547 38-39 21.55883318289981 26.725937474357924 29.641113481578728 22.074115861163534 40-41 22.137532370737077 26.478837236354032 29.68328120028221 21.700349192626682 42-43 21.789753866631873 26.48305345541115 29.545092719563225 22.182099958393753 44-45 21.824827878741633 26.844311640085966 29.59419052037785 21.736669960794543 46-47 22.173905373098933 27.077915124541363 28.781094313039844 21.967085189319857 48-49 21.92420209001771 27.145861870776304 28.758829941249274 22.17110609795671 50-51 22.136376830770587 27.404011155661273 28.582740727292492 21.876871286275644 52-53 22.4332262908572 27.088507542946537 28.59317966195688 21.885086504239382 54-55 22.040432427997043 27.31396159842455 28.693997702469844 21.951608271108558 56-57 22.05385889925744 27.49376892318502 28.454030516638458 21.998341660919085 58-59 22.051237026017304 27.14661000468536 28.42439703648757 22.377755932809766 60-61 21.765875852410417 27.29290634135716 28.746211366812304 22.195006439420123 62-63 21.886350591394915 27.264620556640285 28.867678439040724 21.981350412924076 64-65 21.653447452933154 27.30760213670682 28.721797170379254 22.317153239980776 66-67 21.5800691188708 27.346053191050768 28.587641263666402 22.48623642641203 68-69 22.12988846157397 27.02266364799281 28.66630899416406 22.18113889626916 70-71 21.923026978857436 27.144714851408374 28.306701439625954 22.62555673010824 72-73 21.908459490540825 27.142279236340492 28.385094747656183 22.564166525462497 74-75 21.898829987118372 27.551095758908428 28.165557643236323 22.384516610736878 76-77 21.955375087158032 27.218059144415736 28.287108814240597 22.539456954185635 78-79 22.078441433476623 27.396259320577837 28.137619399475778 22.387679846469762 80-81 22.21189962746371 27.483354815951895 27.88612058773163 22.418624968852765 82-83 22.183662989480315 27.466637901050657 27.975703171655258 22.37399593781377 84-85 22.12628391877254 27.52107035647241 27.927445541500678 22.425200183254372 86-87 22.30595952171573 27.438815806743754 27.704130517528775 22.551094154011732 88-89 22.216361340006944 27.51476983351265 27.536619913650124 22.732248912830286 90-91 22.138470042465865 27.54755678108373 27.60369489356645 22.71027828288395 92-93 22.31692760578484 27.0658902822468 27.813895918605624 22.80328619336273 94-95 22.112131380178436 26.97115511872144 27.881251268325702 23.035462232774417 96-97 22.402789851600886 26.654084130103072 27.841088266993246 23.10203775130279 98-99 22.135597559729714 26.90020929145754 27.45425108656362 23.50994206224913 100-101 22.972999884413976 25.76147684039951 27.688601801212155 23.57692147397436 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 982.0 1 664.5 2 511.5 3 708.5 4 598.5 5 543.0 6 988.5 7 2170.5 8 3685.0 9 4344.0 10 3533.0 11 2569.0 12 2444.5 13 2619.5 14 2832.5 15 3020.5 16 3311.0 17 3663.0 18 4001.5 19 4428.0 20 5037.5 21 5881.0 22 7165.5 23 8898.5 24 10882.0 25 13571.0 26 17498.0 27 22130.0 28 25775.0 29 29049.0 30 32869.0 31 38147.0 32 45135.5 33 48756.0 34 50103.0 35 53107.0 36 57387.0 37 62062.0 38 65519.0 39 68151.0 40 71373.5 41 75543.5 42 77891.5 43 79219.0 44 81415.0 45 84792.0 46 86167.0 47 86427.0 48 86450.5 49 84849.5 50 83783.0 51 81898.0 52 79813.0 53 79126.5 54 78980.5 55 74637.0 56 68265.5 57 62636.0 58 57960.5 59 51435.0 60 41584.5 61 34511.5 62 29093.0 63 22009.5 64 15977.0 65 12336.0 66 9247.0 67 6821.0 68 5284.5 69 4068.0 70 3074.0 71 2249.0 72 1551.0 73 1096.5 74 822.0 75 638.5 76 466.5 77 322.0 78 207.0 79 119.5 80 70.0 81 46.0 82 29.5 83 20.0 84 19.0 85 14.5 86 8.0 87 5.0 88 4.5 89 6.0 90 5.0 91 2.0 92 1.5 93 3.0 94 3.0 95 2.0 96 2.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1473364618581863 2 0.050479038282056035 3 0.15016591241989374 4 0.12822741893419107 5 0.19404289939129907 6 0.19584718670601106 7 0.16242686485395935 8 0.07143337505064307 9 0.12068221743630457 10-11 0.10227028552026625 12-13 0.14950980794181665 14-15 0.13659275102967397 16-17 0.13454242453568305 18-19 0.13282015028073071 20-21 0.13413235923688488 22-23 0.19537561161239317 24-25 0.21526377860410492 26-27 0.14805407613108312 28-29 0.12828892872901082 30-31 0.100076436171696 32-33 0.12484438021910609 34-35 0.09357690118574483 36-37 0.08720038578943311 38-39 0.050684070931455126 40-41 0.05915191935163756 42-43 0.061591807879486725 44-45 0.03957130133402443 46-47 0.05138118193941203 48-49 0.043015849843929144 50-51 0.05365704434774194 52-53 0.027187329310319374 54-55 0.050684070931455126 56-57 0.02683877380634092 58-59 0.05158621458881112 60-61 0.03758248463485325 62-63 0.051791247238210214 64-65 0.03934576541968543 66-67 0.03901771318064689 68-69 0.0649338400646919 70-71 0.05336999863858321 72-73 0.05195527335772949 74-75 0.043302895553087875 76-77 0.021979500015582482 78-79 0.022533088168960026 80-81 0.025362538730667475 82-83 0.022143526135101753 84-85 0.020298232290509942 86-87 0.02411183956933302 88-89 0.06454427803083362 90-91 0.10987699681297249 92-93 0.08022927570986405 94-95 0.0762926488414015 96-97 0.06809134286543789 98-99 0.05597391328595165 100-101 0.13206152947795408 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2438636.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.559918666855 #Duplication Level Percentage of deduplicated Percentage of total 1 78.86688969115718 46.97305535513146 2 12.662243073708398 15.083243352200403 3 3.858445700298147 6.89426136270702 4 1.624199684778518 3.8694880449656015 5 0.8066860173323684 2.402307679100252 6 0.49109689039264426 1.7549814509598773 7 0.32472770850726923 1.3538529135297004 8 0.22459154322602934 1.0701323238244602 9 0.16914744985527363 0.9066967520477427 >10 0.874769746976075 9.428534819532802 >50 0.06195510346757135 2.5399911900738297 >100 0.02994346824707442 3.2946845114104653 >500 0.0029619304974076565 1.2218400687571243 >1k 0.0021353452423128177 2.066280809309664 >5k 1.377642091814721E-4 0.6482538085555843 >10k+ 6.888210459073605E-5 0.49239555789406514 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12002 0.49216037161757636 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9861 0.40436539114488596 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5940 0.2435787874861193 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2716 0.11137373515358587 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2621 0.10747811481500315 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2607 0.10690402339668569 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2540 0.10415658589473789 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2453 0.1005890177951937 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 4.100652987981806E-5 0.0 0.0 4.100652987981806E-5 0.0 12-13 4.100652987981806E-5 0.0 0.0 8.201305975963613E-5 8.201305975963613E-5 14-15 1.230195896394542E-4 0.0 0.0 8.201305975963613E-5 1.0251632469954516E-4 16-17 1.230195896394542E-4 0.0 0.0 1.230195896394542E-4 1.230195896394542E-4 18-19 1.435228545793632E-4 0.0 0.0 1.6402611951927225E-4 1.435228545793632E-4 20-21 2.050326493990903E-4 0.0 0.0 1.6402611951927225E-4 1.6402611951927225E-4 22-23 2.050326493990903E-4 0.0 0.0 3.690587689183626E-4 2.050326493990903E-4 24-25 2.050326493990903E-4 2.050326493990903E-5 0.0 6.3560121313718E-4 2.050326493990903E-4 26-27 2.050326493990903E-4 4.100652987981806E-5 0.0 0.001250699161334451 2.050326493990903E-4 28-29 2.050326493990903E-4 4.100652987981806E-5 0.0 0.004264679107501078 2.460391792789084E-4 30-31 2.050326493990903E-4 8.201305975963613E-5 0.0 0.012035416519726602 2.460391792789084E-4 32-33 2.050326493990903E-4 1.230195896394542E-4 0.0 0.027904943583216192 2.460391792789084E-4 34-35 2.050326493990903E-4 1.230195896394542E-4 0.0 0.05324697904894375 2.460391792789084E-4 36-37 2.050326493990903E-4 1.230195896394542E-4 0.0 0.091280535512475 2.460391792789084E-4 38-39 2.460391792789084E-4 1.230195896394542E-4 0.0 0.14586022678251284 2.870457091587264E-4 40-41 2.6654244421881737E-4 1.230195896394542E-4 0.0 0.20845669464405514 2.870457091587264E-4 42-43 2.870457091587264E-4 1.230195896394542E-4 0.0 0.26980246334426294 2.870457091587264E-4 44-45 2.870457091587264E-4 1.230195896394542E-4 0.0 0.3466897068689218 2.870457091587264E-4 46-47 2.870457091587264E-4 1.230195896394542E-4 0.0 0.4366375301603027 2.870457091587264E-4 48-49 2.870457091587264E-4 1.230195896394542E-4 0.0 0.529886379107009 2.870457091587264E-4 50-51 2.870457091587264E-4 1.845293844591813E-4 0.0 0.6315005601491981 3.280522390385445E-4 52-53 2.870457091587264E-4 2.460391792789084E-4 0.0 0.7272713106835133 3.895620338582716E-4 54-55 2.870457091587264E-4 2.460391792789084E-4 0.0 0.8297876353830584 4.100652987981806E-4 56-57 2.870457091587264E-4 2.460391792789084E-4 0.0 0.9500392842556249 4.100652987981806E-4 58-59 2.870457091587264E-4 2.6654244421881737E-4 0.0 1.0799889774447684 4.100652987981806E-4 60-61 2.870457091587264E-4 2.870457091587264E-4 0.0 1.2163356892951633 4.305685637380896E-4 62-63 2.870457091587264E-4 2.870457091587264E-4 0.0 1.349196846105774 4.920783585578168E-4 64-65 2.870457091587264E-4 2.870457091587264E-4 0.0 1.4858101004003879 4.920783585578168E-4 66-67 2.870457091587264E-4 2.870457091587264E-4 0.0 1.644177318796245 4.920783585578168E-4 68-69 2.870457091587264E-4 2.870457091587264E-4 0.0 1.8200543254507848 4.920783585578168E-4 70-71 2.870457091587264E-4 2.870457091587264E-4 0.0 2.0037840825773094 4.920783585578168E-4 72-73 2.870457091587264E-4 2.870457091587264E-4 0.0 2.196535276277394 5.330848884376347E-4 74-75 2.870457091587264E-4 2.870457091587264E-4 0.0 2.387031110834089 5.740914183174528E-4 76-77 2.870457091587264E-4 2.870457091587264E-4 0.0 2.588557701928455 5.740914183174528E-4 78-79 2.870457091587264E-4 2.870457091587264E-4 0.0 2.8073275388372845 5.740914183174528E-4 80-81 4.100652987981806E-4 2.870457091587264E-4 0.0 3.0451654121402294 5.945946832573619E-4 82-83 4.100652987981806E-4 2.870457091587264E-4 0.0 3.2867553829271774 6.150979481972709E-4 84-85 4.100652987981806E-4 2.870457091587264E-4 0.0 3.5317693989590904 6.150979481972709E-4 86-87 4.100652987981806E-4 2.870457091587264E-4 0.0 3.776516872546784 6.150979481972709E-4 88-89 4.100652987981806E-4 2.870457091587264E-4 0.0 4.041439558835349 6.150979481972709E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4990 0.0 61.65476 1 GTATCAA 9350 0.0 44.36507 1 TGGTATC 1750 0.0 40.49423 2 GTGGTAT 2095 0.0 35.690304 1 ATCAACG 11960 0.0 34.159843 3 TATCAAC 11980 0.0 34.141815 2 TCAACGC 12115 0.0 33.722797 4 CAACGCA 12360 0.0 33.054344 5 AACGCAG 12745 0.0 32.273777 6 ACGCAGA 14280 0.0 28.531717 7 CGCAGAG 14310 0.0 28.369905 8 GCAGAGT 15660 0.0 25.316057 9 GAGTACT 11250 0.0 23.57781 12-13 GTACTTT 11540 0.0 22.694855 14-15 TACGTTG 105 0.0021805123 22.648247 5 CAGAGTA 15180 0.0 22.593182 10-11 AGAGTAC 14765 0.0 21.409231 10-11 GTACATG 8005 0.0 20.584929 1 TAAGGTA 1010 0.0 20.248878 4 AGTACTT 11780 0.0 19.994942 12-13 >>END_MODULE