FastQCFastQC Report
Thu 26 May 2016
SRR938240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2438636
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114630.47005785201235445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99630.4085480571926274No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63100.258751203541652No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28110.11526935549216856No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27770.11387513347625476No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC27590.11313701593841803No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26470.10854428459187841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49500.059.3968471
GTATCAA91950.042.823361
TGGTATC18000.042.747582
GTGGTAT23050.036.2676731
TATCAAC117850.032.9680142
ATCAACG117400.032.9278223
TCAACGC119650.032.3866734
CAACGCA120650.032.039525
AACGCAG124900.031.0633666
ACGCAGA141800.027.1602347
CGCAGAG142700.026.9556588
GCAGAGT158050.024.097329
TAAGGTG17600.024.0140975
GAGTACT110200.022.5821512-13
GTACATG81150.022.241961
TACATGG82450.021.7179972
CAGAGTA153000.021.65030310-11
GTACTTT115550.021.3927414-15
GTAAGGT16500.021.2978944
AGAGTAC148600.020.70939310-11