##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938240_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2438636 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.534214618335824 34.0 31.0 34.0 31.0 34.0 2 32.74439522749603 34.0 31.0 34.0 31.0 34.0 3 32.7990651331318 34.0 31.0 34.0 31.0 34.0 4 36.24870583391699 37.0 35.0 37.0 35.0 37.0 5 36.22371112375935 37.0 37.0 37.0 35.0 37.0 6 36.258095919194176 37.0 37.0 37.0 35.0 37.0 7 36.22057002357055 37.0 37.0 37.0 35.0 37.0 8 36.20628744921341 37.0 37.0 37.0 35.0 37.0 9 37.90116565161836 39.0 38.0 39.0 35.0 39.0 10-11 37.8782735512803 39.0 38.0 39.0 35.0 39.0 12-13 37.80066213243797 39.0 38.0 39.0 35.0 39.0 14-15 39.08243604211535 40.0 38.5 41.0 36.0 41.0 16-17 38.97375684604016 40.0 38.0 41.0 36.0 41.0 18-19 39.227855243669 40.5 39.0 41.0 36.0 41.0 20-21 39.21292517620506 41.0 39.0 41.0 36.0 41.0 22-23 39.121263895062654 40.0 39.0 41.0 35.5 41.0 24-25 39.02606190509777 40.0 39.0 41.0 35.0 41.0 26-27 38.858791143901755 40.0 38.0 41.0 35.0 41.0 28-29 38.73910989585981 40.0 38.0 41.0 35.0 41.0 30-31 38.487643912416615 40.0 38.0 41.0 34.0 41.0 32-33 38.25836758745463 40.0 38.0 41.0 34.0 41.0 34-35 38.09858256828818 40.0 38.0 41.0 34.0 41.0 36-37 38.083391904326845 40.0 38.0 41.0 34.0 41.0 38-39 37.93009063263234 40.0 38.0 41.0 33.0 41.0 40-41 37.750810494063074 40.0 37.0 41.0 33.0 41.0 42-43 37.51085688885098 40.0 37.0 41.0 33.0 41.0 44-45 37.22421652924012 40.0 37.0 41.0 32.0 41.0 46-47 36.82780066397773 40.0 36.0 41.0 31.0 41.0 48-49 36.41844867376681 39.5 36.0 41.0 30.0 41.0 50-51 36.034925056465994 39.0 35.0 41.0 29.5 41.0 52-53 35.51968744002795 39.0 35.0 40.0 28.0 41.0 54-55 35.21583417943474 38.0 35.0 40.0 27.0 41.0 56-57 34.88107306707521 38.0 34.0 40.0 26.0 41.0 58-59 34.448842098615785 38.0 34.0 40.0 25.5 41.0 60-61 33.98042348263537 37.0 33.0 40.0 24.0 41.0 62-63 33.52887228762308 37.0 33.0 39.5 22.5 41.0 64-65 33.05475437908733 36.0 32.0 39.0 22.0 40.5 66-67 32.51852162438347 35.5 31.5 39.0 19.5 40.0 68-69 31.94312824874233 35.0 31.0 38.0 17.5 40.0 70-71 31.34169224107247 35.0 30.5 37.5 12.5 40.0 72-73 30.763526413946156 34.5 30.0 37.0 8.0 39.0 74-75 30.170013482947027 34.0 29.0 36.5 7.0 39.0 76-77 28.82303857566279 32.5 28.0 35.0 4.0 37.0 78-79 29.49911938477083 34.0 29.0 35.0 2.0 37.5 80-81 29.167813482618975 34.0 29.0 35.0 2.0 37.0 82-83 28.603464805735662 34.0 28.5 35.0 2.0 36.5 84-85 28.007412955439023 33.0 27.0 35.0 2.0 36.0 86-87 27.36682493820316 33.0 26.0 35.0 2.0 36.0 88-89 26.629951128417687 32.0 25.0 35.0 2.0 35.0 90-91 26.086537105168627 32.0 24.0 34.5 2.0 35.0 92-93 25.281019389527586 31.5 20.5 34.0 2.0 35.0 94-95 24.463565288136483 31.0 15.5 34.0 2.0 35.0 96-97 23.4310430092888 31.0 2.0 34.0 2.0 35.0 98-99 22.001292115756513 30.5 2.0 34.0 2.0 35.0 100-101 17.631136627196515 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1294037521084519 1101 2 0.12283096638229551 1101 3 0.1678059585825764 1101 4 0.13023438160362844 1101 5 0.09414628686385385 1101 6 0.08194359964401343 1101 7 0.11198552566479236 1101 8 0.08891973543401832 1101 9 0.0158550214257005 1101 10-11 0.11688822067836924 1101 12-13 0.19958437427271747 1101 14-15 0.14506387367621443 1101 16-17 0.17160937554816513 1101 18-19 0.20517750286265368 1101 20-21 0.1758305010418013 1101 22-23 0.26610303138756564 1101 24-25 0.31634551321284476 1101 26-27 0.3499270109187478 1101 28-29 0.33080519217221394 1101 30-31 0.2907577726040387 1101 32-33 0.31277160018081673 1101 34-35 0.2728055259763025 1101 36-37 0.2905870483555617 1101 38-39 0.2935926226247787 1101 40-41 0.45648127017289397 1101 42-43 0.27030905965111884 1101 44-45 0.4291429933480373 1101 46-47 0.4258244413097998 1101 48-49 0.33668332850864147 1101 50-51 0.27257010277654103 1101 52-53 0.2239354619540066 1101 54-55 0.23049617537322575 1101 56-57 0.45955418798214254 1101 58-59 0.41902643142969964 1101 60-61 0.41054428667466425 1101 62-63 0.33121797135412123 1101 64-65 0.25818744778163705 1101 66-67 0.07915632171337705 1101 68-69 0.20061418371176387 1101 70-71 0.25288442637789643 1101 72-73 0.24602144986797825 1101 74-75 0.1870976153617363 1101 76-77 0.057638592018310675 1101 78-79 0.0978372504340328 1101 80-81 0.0032256353088691014 1101 82-83 0.07943766230694749 1101 84-85 0.04287494668200864 1101 86-87 -0.29325692901440803 1101 88-89 -0.36032799129585413 1101 90-91 -0.2979226725963038 1101 92-93 -0.48297603013500634 1101 94-95 -0.7538609132708309 1101 96-97 -0.8895330289242303 1101 98-99 -0.806515450097379 1101 100-101 -1.1175103892437193 1102 1 0.13316831723273737 1102 2 0.1525637349390223 1102 3 0.06800784190111386 1102 4 0.06876455907094936 1102 5 0.03804062247068174 1102 6 0.02185890078607855 1102 7 0.05570043882234188 1102 8 0.08248852068893342 1102 9 0.12500602922220594 1102 10-11 0.16236949715227667 1102 12-13 0.14456042446163053 1102 14-15 0.17986918378719707 1102 16-17 0.143062800985021 1102 18-19 0.1924066581730557 1102 20-21 0.1373012934255584 1102 22-23 0.14399227836212702 1102 24-25 0.13632605447350699 1102 26-27 0.10398280689206274 1102 28-29 0.04567569831764473 1102 30-31 0.04357304088137681 1102 32-33 0.14746048407840107 1102 34-35 0.1518479038395455 1102 36-37 0.2028521157084171 1102 38-39 0.18887284235776036 1102 40-41 0.0882499765612792 1102 42-43 0.22193916991216156 1102 44-45 0.07401482863029685 1102 46-47 0.06495145190476137 1102 48-49 0.25788028344076963 1102 50-51 0.2155682078197927 1102 52-53 0.19226784049833867 1102 54-55 0.22593291584482955 1102 56-57 0.23121134827141532 1102 58-59 0.22026096368264803 1102 60-61 0.1499192925984687 1102 62-63 0.18623361130484284 1102 64-65 0.05603378325444908 1102 66-67 -0.22553617047105945 1102 68-69 -0.09658664974298148 1102 70-71 -0.15883532016475144 1102 72-73 -0.13550010981125737 1102 74-75 -0.09455606448469212 1102 76-77 0.05479582625872581 1102 78-79 -0.04987801186990737 1102 80-81 -0.0727425791445988 1102 82-83 0.03342583320969439 1102 84-85 -0.19152406195385296 1102 86-87 -0.27099454858987926 1102 88-89 -0.23168837715551405 1102 90-91 -0.5326226656971365 1102 92-93 -0.69524369845211 1102 94-95 -0.5215257036430287 1102 96-97 -0.5156352701839424 1102 98-99 -0.5951426898196566 1102 100-101 -1.256359339126444 1103 1 0.09229075765036043 1103 2 0.05690936697201465 1103 3 0.026614827261468577 1103 4 0.059321057422828005 1103 5 0.013480005995774036 1103 6 0.06764039240563591 1103 7 0.012040822508495808 1103 8 0.01884669171263198 1103 9 0.06052003383265259 1103 10-11 0.09305161459666778 1103 12-13 0.0961579425529635 1103 14-15 0.08943812202829804 1103 16-17 0.15571383791125015 1103 18-19 0.16420284185331013 1103 20-21 0.14629300264184764 1103 22-23 0.19917047960010592 1103 24-25 0.1895730499689492 1103 26-27 0.21594955547837458 1103 28-29 0.19890431653522 1103 30-31 0.10672477374620826 1103 32-33 0.20227739991705107 1103 34-35 0.1819674346629725 1103 36-37 0.15839671657663956 1103 38-39 0.07220948968443253 1103 40-41 0.12024108246809107 1103 42-43 0.15703586601289032 1103 44-45 0.19532343087279003 1103 46-47 0.20696558033901624 1103 48-49 0.047252487192579906 1103 50-51 0.012701310029491708 1103 52-53 -0.23692181279712798 1103 54-55 -0.07186917445381624 1103 56-57 -0.07456926055973412 1103 58-59 0.1436420390335229 1103 60-61 0.04695242326536686 1103 62-63 -0.042057486474362804 1103 64-65 -0.07733156530585461 1103 66-67 -0.06979298465322614 1103 68-69 0.05049290158829933 1103 70-71 -0.2845436464509845 1103 72-73 -0.3852141054664955 1103 74-75 -0.49213255565967273 1103 76-77 -0.3394966283832481 1103 78-79 -0.3780866453283096 1103 80-81 -0.23782454159171706 1103 82-83 -0.15563996232590327 1103 84-85 -0.4257665541673852 1103 86-87 -0.5989355868127113 1103 88-89 -0.6609304460125855 1103 90-91 -0.8100328743386598 1103 92-93 -0.5676717549106733 1103 94-95 -0.6061339694709922 1103 96-97 -0.8271184586883393 1103 98-99 -1.0640838169579538 1103 100-101 -1.5869374955034985 1104 1 0.0802310997282234 1104 2 0.10934695698021812 1104 3 0.14659946003198598 1104 4 0.13140055838934472 1104 5 0.08930631257796051 1104 6 0.04125912055076242 1104 7 4.456396937158047E-4 1104 8 0.037570384872068985 1104 9 0.10021466877747542 1104 10-11 0.11188134004954264 1104 12-13 0.12048336053086928 1104 14-15 0.20472613038209175 1104 16-17 0.12263056986678578 1104 18-19 0.1759943881281174 1104 20-21 0.1665347363960521 1104 22-23 0.1526489350703244 1104 24-25 0.15312039959885482 1104 26-27 0.16140572886334326 1104 28-29 0.01654655980092201 1104 30-31 0.13260818075558234 1104 32-33 0.056359176155716284 1104 34-35 0.011610208189523519 1104 36-37 0.10659432658688672 1104 38-39 0.14035267839647503 1104 40-41 0.16257777981753918 1104 42-43 0.11803814247754474 1104 44-45 0.14136342780411582 1104 46-47 0.19515048237391852 1104 48-49 0.22798229544405046 1104 50-51 0.13650757720384377 1104 52-53 0.1274258173065803 1104 54-55 0.24031722534934374 1104 56-57 0.31391875272301917 1104 58-59 0.2807738923269625 1104 60-61 0.08425611446116932 1104 62-63 0.11558282098151551 1104 64-65 0.07157851811681581 1104 66-67 0.15890287179951912 1104 68-69 0.1774342266759561 1104 70-71 -0.07030137289601868 1104 72-73 -0.178278420859602 1104 74-75 -0.040491827652083145 1104 76-77 -0.23285338223290353 1104 78-79 -0.26306804048671495 1104 80-81 -0.31758497419552256 1104 82-83 -0.24761020729925676 1104 84-85 -0.6300981373219479 1104 86-87 -0.6811049498994812 1104 88-89 -0.6131075960632835 1104 90-91 -0.7110754291624239 1104 92-93 -0.7664638215088893 1104 94-95 -0.7987734799456305 1104 96-97 -0.8053939621877042 1104 98-99 -0.8516877176743591 1104 100-101 -1.2532780159779655 1105 1 0.10613754258840657 1105 2 0.05614267001608653 1105 3 0.035103367482740566 1105 4 0.018995215053877246 1105 5 -0.04293591981191014 1105 6 0.05386670845362573 1105 7 0.057526711907662786 1105 8 0.09795040071468719 1105 9 0.07757539564607896 1105 10-11 0.05907145585432261 1105 12-13 0.1149279158144978 1105 14-15 0.07219885655314329 1105 16-17 0.03860615421096725 1105 18-19 0.1178051087897245 1105 20-21 0.10582959227555477 1105 22-23 0.18537050999169935 1105 24-25 0.06585615454671512 1105 26-27 0.01764490356953985 1105 28-29 0.04080981173396481 1105 30-31 0.07007086489642234 1105 32-33 0.08373590081344418 1105 34-35 0.1285795397257843 1105 36-37 0.16366209809855548 1105 38-39 0.06615563440366401 1105 40-41 0.0931248377021987 1105 42-43 0.0784147961981887 1105 44-45 -0.004134432260670451 1105 46-47 -0.04673849075274461 1105 48-49 0.09638440818671512 1105 50-51 0.056560750455837194 1105 52-53 -0.18800199978215204 1105 54-55 -0.12657950652032213 1105 56-57 -0.2577297289279201 1105 58-59 -0.12791881859261878 1105 60-61 -0.3623275262661707 1105 62-63 -0.38986363186896256 1105 64-65 -0.45003272156775154 1105 66-67 -0.425082195945393 1105 68-69 -0.3445320678666164 1105 70-71 -0.528442400242259 1105 72-73 -0.28809973331378913 1105 74-75 -0.24185300674455945 1105 76-77 -0.2859675199927132 1105 78-79 -0.41569230599237983 1105 80-81 -0.3759654511291508 1105 82-83 -0.49257977085887816 1105 84-85 -0.5162029305994729 1105 86-87 -0.5151400800099033 1105 88-89 -0.790007121080432 1105 90-91 -0.8640636166609461 1105 92-93 -0.8594407181890347 1105 94-95 -0.8662017596032427 1105 96-97 -1.0028284783968253 1105 98-99 -1.1311234649373745 1105 100-101 -1.3770432674499098 1106 1 0.24144609636849879 1106 2 0.2535436601150991 1106 3 0.22722613912284118 1106 4 0.16003992401319067 1106 5 0.17163451165208699 1106 6 0.19382730200332787 1106 7 0.24037666316726813 1106 8 0.1843391674871171 1106 9 0.20687522234689482 1106 10-11 0.21848589814315034 1106 12-13 0.2068791204668159 1106 14-15 0.28202834879291316 1106 16-17 0.31643307722663394 1106 18-19 0.3113091569503297 1106 20-21 0.39420986611374786 1106 22-23 0.291179576660916 1106 24-25 0.3266141831729428 1106 26-27 0.36314690973058106 1106 28-29 0.3003109036143812 1106 30-31 0.22399155981339902 1106 32-33 0.3577147616580234 1106 34-35 0.44781001489685224 1106 36-37 0.48068432276310347 1106 38-39 0.3555828909565335 1106 40-41 0.45860791713948856 1106 42-43 0.46142560270837407 1106 44-45 0.39194658510747615 1106 46-47 0.4278976884781116 1106 48-49 0.5634109732949639 1106 50-51 0.41036783724559456 1106 52-53 0.4369867509027543 1106 54-55 0.3309089335640323 1106 56-57 0.4535157832922181 1106 58-59 0.49593882839312187 1106 60-61 0.2684372997338045 1106 62-63 0.41803758839301963 1106 64-65 0.268203692445276 1106 66-67 0.07734128481967417 1106 68-69 -0.03391528285317236 1106 70-71 -0.4827887971165019 1106 72-73 -0.4742364756077073 1106 74-75 -0.15678050938864985 1106 76-77 -0.15448632167982979 1106 78-79 -0.17412959096122194 1106 80-81 -0.25774251638393864 1106 82-83 -0.1565578512200041 1106 84-85 -0.31370420884023176 1106 86-87 -0.5301021668315222 1106 88-89 -0.7611930457781106 1106 90-91 -1.0604271809855241 1106 92-93 -0.7565804653442534 1106 94-95 -0.8020187426046306 1106 96-97 -0.8493497919664073 1106 98-99 -0.7358562807623912 1106 100-101 -1.2345767645254853 1107 1 0.026653094620918694 1107 2 0.05693992552439653 1107 3 0.005481832050904245 1107 4 0.029309749002209173 1107 5 -0.004620316326708007 1107 6 -0.0013776787631272214 1107 7 -0.0467624378431708 1107 8 0.025525842894396078 1107 9 0.018174310177151654 1107 10-11 0.04029359108870523 1107 12-13 -0.0014265795051358054 1107 14-15 0.01842549063310628 1107 16-17 0.0013467836837008917 1107 18-19 0.016204084567775112 1107 20-21 -0.016385890021581417 1107 22-23 0.03256630575847197 1107 24-25 0.09059547941952673 1107 26-27 0.05662235645245772 1107 28-29 0.022632663649467588 1107 30-31 0.09922080508593467 1107 32-33 0.0375185060172285 1107 34-35 0.1451201429497999 1107 36-37 0.08066770174046667 1107 38-39 0.04683500252806283 1107 40-41 -0.003130379391585336 1107 42-43 0.11677831010347717 1107 44-45 0.04854842067343412 1107 46-47 0.1324272998687519 1107 48-49 -0.05330341502497049 1107 50-51 -0.052344939637038124 1107 52-53 0.01610300092303163 1107 54-55 0.06090114974958993 1107 56-57 0.034952415552275795 1107 58-59 0.018832920220987148 1107 60-61 0.05736024470928669 1107 62-63 0.09940174060149332 1107 64-65 -0.15108875863980842 1107 66-67 -0.11675717687916887 1107 68-69 -0.09906021470789028 1107 70-71 -0.19664021428649292 1107 72-73 -0.46101064311226025 1107 74-75 -0.43428916753813596 1107 76-77 -0.5247210589975033 1107 78-79 -0.32947273033341773 1107 80-81 -0.3760349906145173 1107 82-83 -0.32465097341532 1107 84-85 -0.4695823373786929 1107 86-87 -0.5692937910024973 1107 88-89 -0.6778535615729133 1107 90-91 -0.8308421069554548 1107 92-93 -0.7942290863552302 1107 94-95 -0.6641449905709678 1107 96-97 -1.0130339692084753 1107 98-99 -1.1213067259670524 1107 100-101 -1.625504583907457 1108 1 -0.06903140867806457 1108 2 -0.019474909455382772 1108 3 -0.010505064100890138 1108 4 -0.01357310944641199 1108 5 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100-101 3.4234026762520102 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 9.0 4 19.0 5 30.0 6 69.0 7 213.0 8 575.0 9 1145.0 10 1973.0 11 3332.0 12 5046.0 13 7800.0 14 10712.0 15 14431.0 16 18999.0 17 26476.0 18 26969.0 19 20832.0 20 19037.0 21 19389.0 22 21094.0 23 22788.0 24 25890.0 25 29143.0 26 33475.0 27 39346.0 28 47007.0 29 57096.0 30 68214.0 31 83813.0 32 104308.0 33 134545.0 34 182245.0 35 260408.0 36 374784.0 37 480347.0 38 281802.0 39 15272.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.026703554212965 19.223901555198854 15.24736282204493 22.50203206854325 2 15.84667091828888 20.172785379887912 40.30923374420956 23.67130995761365 3 19.677332042880224 24.103066739938157 29.88968266336398 26.329918553817638 4 13.23076229119979 17.33080186251735 37.138809210890514 32.29962663539234 5 13.770577850861457 37.36127827179884 34.25175458920161 14.616389288138096 6 31.80134274204131 36.66119629687951 17.171384142980866 14.36607681809831 7 28.185592273713667 33.02087724449241 20.36646715622996 18.427063325563964 8 26.277189379636813 35.055867296308264 19.404207926070146 19.262735397984777 9 26.02356399233014 17.031447087634234 20.564569702079357 36.380419217956266 10-11 24.964529351653958 26.804451340831513 27.017254727642833 21.2137645798717 12-13 25.856868872807826 24.202057969742686 27.606508115050453 22.33456504239903 14-15 22.501451631157746 25.70084260217597 26.295232252783933 25.50247351388235 16-17 21.681491321830514 28.77991799673701 27.254654024514043 22.28393665691843 18-19 21.595904431821722 26.90608602513864 30.076690412181232 21.4213191308584 20-21 22.11738447230337 26.078943310932836 30.525322352331386 21.27834986443241 22-23 21.832922174527074 26.124481062364367 30.3642692062284 21.67832755688016 24-25 21.6821242212872 26.260236832441446 30.44512549314462 21.612513453126734 26-27 21.278255946081124 26.662986328130206 30.207246671340915 21.85151105444775 28-29 21.411422156379274 26.980730685831748 30.084264162356405 21.523582995432573 30-31 21.613638921974346 26.446468916678167 30.096752148458684 21.843140012888803 32-33 21.284295811264986 26.538441981501133 30.000828331903573 22.176433875330307 34-35 22.02164525243909 26.73246090690245 29.776186121283953 21.46970771937451 36-37 22.05076115904243 26.25753167831022 29.80255403012323 21.889153132524118 38-39 21.51561278745236 26.688032943789374 29.6925298268089 22.103824441949367 40-41 22.154548891697427 26.470888552994058 29.691350835768482 21.68321171954003 42-43 21.77915827463955 26.511820737894208 29.602724870616008 22.10629611685023 44-45 21.83551006290198 26.83195172054208 29.626219252528717 21.70631896402722 46-47 22.164635302777274 27.040667259211876 28.832306630867915 21.96239080714294 48-49 21.899758490507235 27.121809236000438 28.77682279797123 22.2016094755211 50-51 22.12362670653774 27.360723781349105 28.661837384174422 21.85381212793874 52-53 22.46428846781615 27.085380417219618 28.561966972164033 21.8883641428002 54-55 22.044385353829774 27.306439220657307 28.66402877742097 21.98514664809195 56-57 22.03086611021349 27.447210396185284 28.457406068023232 22.064517425577993 58-59 22.007008350588976 27.136450785378646 28.45327995278274 22.40326091124964 60-61 21.80263677779922 27.321523146286648 28.699394249315525 22.176445826598613 62-63 21.911224583961364 27.310857506126506 28.784828816635393 21.993089093276733 64-65 21.667713910487723 27.349038982899305 28.64334211322866 22.33990499338432 66-67 21.565740950356147 27.455330045834685 28.467232464213183 22.511696539595984 68-69 22.084461344096606 27.143261468793067 28.59544201614491 22.176835170965415 70-71 21.910763266859593 27.28214346647768 28.218160849393993 22.588932417268737 72-73 21.933784252891687 27.256547420472067 28.277072961086503 22.532595365549742 74-75 21.944877016655752 27.675589570746645 28.01753465692911 22.3619987556685 76-77 21.878226074701995 27.315811513772175 28.367698996899687 22.438263414626146 78-79 22.036706207397803 27.524815584028854 28.195232676157783 22.24324553241556 80-81 22.135711540898544 27.62388126788341 27.913993937736098 22.326413253481945 82-83 22.11729245092236 27.668089658756735 28.040450878058724 22.17416701226218 84-85 22.076981128979998 27.703957541230366 28.01358007981527 22.205481249974365 86-87 22.217992721201934 27.68654611905111 27.71263411887883 22.382827040868122 88-89 22.12459469308646 27.86007588219045 27.47972158073336 22.535607843989727 90-91 22.05864498324851 27.817708179518224 27.685181083449002 22.43846575378426 92-93 22.10938309101517 27.45140904788907 27.87190343230797 22.56730442878779 94-95 21.874871195882534 27.381873936116442 27.92727542662487 22.815979441376157 96-97 22.203593467623048 27.105418951144156 27.856746061935734 22.834241519297063 98-99 21.838781598650414 27.366437203785882 27.512907671521468 23.281873526042236 100-101 22.44861677342414 26.8581602719496 27.573139358585756 23.12008359604051 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 936.0 1 640.5 2 495.5 3 709.5 4 581.5 5 456.0 6 979.5 7 2315.5 8 3734.5 9 4366.0 10 3617.5 11 2520.0 12 2341.5 13 2468.5 14 2690.0 15 2916.0 16 3137.5 17 3443.5 18 3724.0 19 4150.5 20 4801.0 21 5690.5 22 6915.0 23 8477.0 24 10443.0 25 13274.0 26 17086.5 27 21586.5 28 25306.0 29 28661.0 30 32740.0 31 38330.0 32 45678.5 33 49346.0 34 50622.5 35 53632.0 36 58097.5 37 62520.0 38 65349.5 39 68060.0 40 71628.0 41 76072.0 42 78586.0 43 80185.5 44 82805.0 45 85966.5 46 86838.0 47 87647.0 48 87781.5 49 85838.5 50 84856.0 51 82847.5 52 80783.0 53 80447.5 54 80642.5 55 75625.0 56 68705.5 57 62740.5 58 57334.0 59 50629.5 60 40664.0 61 33411.0 62 28039.0 63 20644.5 64 14269.0 65 10846.5 66 8096.0 67 5998.0 68 4546.0 69 3390.0 70 2518.0 71 1675.5 72 1147.0 73 948.0 74 704.5 75 491.0 76 351.0 77 222.5 78 139.5 79 84.5 80 48.5 81 27.5 82 17.5 83 10.5 84 7.5 85 4.0 86 1.0 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009595527991877427 2 0.01439329198781614 3 0.008857410454040702 4 4.100652987981806E-5 5 0.00127120242627436 6 0.0018042873147119949 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.791240677165432E-4 14-15 0.0 16-17 0.0030549864760464457 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0031369995358060815 26-27 0.01863746783037731 28-29 0.007114632934148434 30-31 0.0035060583047244443 32-33 0.0 34-35 0.009267475752838882 36-37 7.996273326564523E-4 38-39 0.0023783787330294475 40-41 0.0017222742549523587 42-43 0.002521901587608811 44-45 2.050326493990903E-5 46-47 1.435228545793632E-4 48-49 6.3560121313718E-4 50-51 0.004490215021840078 52-53 0.008529358215002157 54-55 0.008057783121384249 56-57 0.016218082567468043 58-59 0.022328055519560935 60-61 0.0037520974840033524 62-63 0.018904010274596126 64-65 0.02009319964111085 66-67 0.012630011202983963 68-69 0.029360675393949733 70-71 0.015582481354330864 72-73 0.005638397858474984 74-75 0.015541474824451045 76-77 0.020298232290509942 78-79 0.024665427722710565 80-81 0.01119478265719033 82-83 0.033420321852051724 84-85 0.02074930411918794 86-87 0.030078289666846548 88-89 0.021097859623166394 90-91 0.02640820524260283 92-93 0.022697114288479297 94-95 0.01396272342407805 96-97 0.014372788722876232 98-99 0.010682201033692605 100-101 0.009964586760795788 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2438636.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.46110419907776 #Duplication Level Percentage of deduplicated Percentage of total 1 77.05012196593248 44.27385086836097 2 13.60965674821799 15.640518090460716 3 4.266880506723979 7.355389962056408 4 1.8105964415876001 4.16155483170178 5 0.9053697738419186 2.6011773456712977 6 0.5252063461279848 1.8107361948526222 7 0.35051900227095856 1.4098846239273826 8 0.24694802577356298 1.1351924992584994 9 0.17184485951272008 0.8886955840682659 >10 0.956561675447777 9.940414449523228 >50 0.066313162148543 2.6162742246866473 >100 0.03455299151604764 3.6771798415054118 >500 0.0029285333794531396 1.2090069679098194 >1k 0.0022856845888391004 2.141752744895649 >5k 1.4285528680244377E-4 0.6678945713678189 >10k+ 7.142764340122189E-5 0.470477199753537 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11463 0.47005785201235445 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9963 0.4085480571926274 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6310 0.258751203541652 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2811 0.11526935549216856 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2777 0.11387513347625476 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2759 0.11313701593841803 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2647 0.10854428459187841 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.201305975963613E-5 2 4.100652987981806E-5 0.0 0.0 0.0 8.201305975963613E-5 3 4.100652987981806E-5 0.0 0.0 0.0 8.201305975963613E-5 4 4.100652987981806E-5 0.0 0.0 0.0 8.201305975963613E-5 5 4.100652987981806E-5 0.0 0.0 0.0 8.201305975963613E-5 6 4.100652987981806E-5 0.0 0.0 0.0 8.201305975963613E-5 7 4.100652987981806E-5 0.0 0.0 0.0 8.201305975963613E-5 8 4.100652987981806E-5 0.0 0.0 0.0 8.201305975963613E-5 9 4.100652987981806E-5 0.0 0.0 4.100652987981806E-5 8.201305975963613E-5 10-11 4.100652987981806E-5 0.0 0.0 4.100652987981806E-5 8.201305975963613E-5 12-13 4.100652987981806E-5 0.0 0.0 4.100652987981806E-5 2.050326493990903E-4 14-15 4.100652987981806E-5 0.0 0.0 4.100652987981806E-5 2.460391792789084E-4 16-17 4.100652987981806E-5 0.0 0.0 6.15097948197271E-5 2.460391792789084E-4 18-19 4.100652987981806E-5 0.0 0.0 1.230195896394542E-4 2.460391792789084E-4 20-21 4.100652987981806E-5 0.0 0.0 2.050326493990903E-4 2.460391792789084E-4 22-23 4.100652987981806E-5 0.0 0.0 3.280522390385445E-4 2.460391792789084E-4 24-25 8.201305975963613E-5 0.0 0.0 5.535881533775438E-4 2.460391792789084E-4 26-27 1.0251632469954516E-4 4.100652987981806E-5 0.0 0.0011276795716949968 2.460391792789084E-4 28-29 1.230195896394542E-4 4.100652987981806E-5 0.0 0.004059646458101988 2.460391792789084E-4 30-31 1.230195896394542E-4 6.15097948197271E-5 0.0 0.011789377340447695 2.460391792789084E-4 32-33 1.435228545793632E-4 8.201305975963613E-5 0.0 0.02776142072863683 2.460391792789084E-4 34-35 2.050326493990903E-4 1.230195896394542E-4 0.0 0.05355452802304239 2.460391792789084E-4 36-37 2.050326493990903E-4 1.6402611951927225E-4 0.0 0.09212116937501127 2.460391792789084E-4 38-39 2.460391792789084E-4 1.6402611951927225E-4 0.0 0.14676237043986884 2.870457091587264E-4 40-41 2.460391792789084E-4 1.6402611951927225E-4 0.0 0.20915380565201203 2.870457091587264E-4 42-43 2.870457091587264E-4 1.6402611951927225E-4 0.0 0.27109416903547723 2.870457091587264E-4 44-45 3.280522390385445E-4 1.6402611951927225E-4 0.0 0.34830946479917463 3.280522390385445E-4 46-47 4.100652987981806E-4 1.6402611951927225E-4 0.0 0.4387288631841734 3.6905876891836254E-4 48-49 4.100652987981806E-4 1.6402611951927225E-4 0.0 0.5322442545750985 3.6905876891836254E-4 50-51 4.100652987981806E-4 1.6402611951927225E-4 0.0 0.634042965001747 3.6905876891836254E-4 52-53 4.100652987981806E-4 1.6402611951927225E-4 0.0 0.7305518330738987 4.100652987981806E-4 54-55 4.100652987981806E-4 1.6402611951927225E-4 0.0 0.8335602361320016 4.100652987981806E-4 56-57 4.100652987981806E-4 2.050326493990903E-4 0.0 0.9550830874308425 4.305685637380896E-4 58-59 4.100652987981806E-4 2.050326493990903E-4 0.0 1.0866730418151787 4.5107182867799866E-4 60-61 4.100652987981806E-4 2.050326493990903E-4 0.0 1.2246600148607665 4.5107182867799866E-4 62-63 4.305685637380896E-4 2.050326493990903E-4 0.0 1.3588948904223508 4.5107182867799866E-4 64-65 4.920783585578168E-4 2.050326493990903E-4 0.0 1.4955491512468444 4.715750936179077E-4 66-67 4.920783585578168E-4 2.050326493990903E-4 0.0 1.6553926047183753 5.125816234977258E-4 68-69 5.330848884376348E-4 2.050326493990903E-4 0.0 1.8331149052175069 5.330848884376348E-4 70-71 5.740914183174528E-4 2.050326493990903E-4 0.0 2.0194075704615204 5.535881533775438E-4 72-73 6.150979481972709E-4 2.050326493990903E-4 0.0 2.2132454372034203 6.56104478077089E-4 74-75 6.150979481972709E-4 2.050326493990903E-4 0.0 2.4047664350071107 7.586208027766341E-4 76-77 6.150979481972709E-4 2.050326493990903E-4 0.0 2.6086714048345057 7.791240677165432E-4 78-79 6.150979481972709E-4 2.050326493990903E-4 0.0 2.8318289404404755 8.201305975963613E-4 80-81 6.56104478077089E-4 2.050326493990903E-4 0.0 3.073787969996342 8.201305975963613E-4 82-83 6.56104478077089E-4 2.050326493990903E-4 0.0 3.3198886590700702 9.021436573559973E-4 84-85 6.56104478077089E-4 2.050326493990903E-4 0.0 3.570336040311059 9.226469222959064E-4 86-87 6.56104478077089E-4 2.050326493990903E-4 0.0 3.8212549966456657 9.636534521757245E-4 88-89 6.56104478077089E-4 2.050326493990903E-4 0.0 4.093415335458019 9.841567171156335E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4950 0.0 59.396847 1 GTATCAA 9195 0.0 42.82336 1 TGGTATC 1800 0.0 42.74758 2 GTGGTAT 2305 0.0 36.267673 1 TATCAAC 11785 0.0 32.968014 2 ATCAACG 11740 0.0 32.927822 3 TCAACGC 11965 0.0 32.386673 4 CAACGCA 12065 0.0 32.03952 5 AACGCAG 12490 0.0 31.063366 6 ACGCAGA 14180 0.0 27.160234 7 CGCAGAG 14270 0.0 26.955658 8 GCAGAGT 15805 0.0 24.09732 9 TAAGGTG 1760 0.0 24.014097 5 GAGTACT 11020 0.0 22.58215 12-13 GTACATG 8115 0.0 22.24196 1 TACATGG 8245 0.0 21.717997 2 CAGAGTA 15300 0.0 21.650303 10-11 GTACTTT 11555 0.0 21.39274 14-15 GTAAGGT 1650 0.0 21.297894 4 AGAGTAC 14860 0.0 20.709393 10-11 >>END_MODULE