FastQCFastQC Report
Thu 26 May 2016
SRR938239_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938239_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2984692
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133510.4473158369439794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109690.3675086072532777No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67900.22749416020145463No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA55040.1844076373709582No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC50030.16762198578613807No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA48270.1617252299399737No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA47930.1605860839242374No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA43870.1469833403245628No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA38820.13006367156142074No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG38210.1280199095920115No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA35570.11917477582276496No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA33660.11277545555789341No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT32000.10721374265753383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77400.046.274531
GTATCAA126800.038.3263931
TATCAAC156200.030.6704162
ATCAACG155300.030.6339383
TCAACGC157000.030.33254
CAACGCA159150.029.8630055
AACGCAG165100.028.9263486
ACGCAGA182300.025.8009557
TAAGGTG23850.025.7064155
CGCAGAG184500.025.4675488
AGGTAAG22150.024.2462652
TAAGGTA13300.023.942164
GAGTACT124100.023.59882212-13
GCAGAGT196300.023.5240129
GTAAGGT25700.023.1162344
GTACATG89900.022.9174941
AAGGTAA23100.022.9168031
TACATGG90800.022.1932132
GTACTTT131750.022.17578314-15
AGAGTAC161900.021.65510710-11
Produced by FastQC (version 0.11.5)