FastQCFastQC Report
Thu 26 May 2016
SRR938239_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938239_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2984692
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127400.4268447129553066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109020.3652638195163856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69430.23262031727226798No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA54900.1839385772468315No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC52940.17737173550905755No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA49140.1646401035684754No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA48280.1617587342345542No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA46350.1552924053805217No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG38450.12882401266194302No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA37400.12530606173099268No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA36190.12125204208675469No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA35410.1186387071094773No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT32490.10885545309197732No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA30130.1009484395709842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76050.044.2771571
GTATCAA126550.037.7326431
ATCAACG158450.029.8904613
TCAACGC159300.029.7593234
TATCAAC159750.029.6526932
CAACGCA162800.029.0314275
AACGCAG168150.028.132216
GTGTAAG12900.027.6514071
TAAGGTG26200.027.0409135
AGGTAAG25650.025.217222
ACGCAGA187400.025.1410527
CGCAGAG187200.025.1171728
AAGGTAA25000.024.921661
GTAAGGT29200.024.1003044
GGTAAGG28150.023.8180523
GCAGAGT204550.022.661669
GAGTACT130800.022.51258312-13
TAAGGTA15450.022.4666374
AGAGTAC173300.021.63689410-11
GTACTTT135300.021.5532114-15
Produced by FastQC (version 0.11.5)