##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938239_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2984692 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50452877549844 33.0 31.0 34.0 31.0 34.0 2 32.72932852032974 34.0 31.0 34.0 31.0 34.0 3 32.79250656349131 34.0 31.0 34.0 31.0 34.0 4 36.25035615065139 37.0 35.0 37.0 35.0 37.0 5 36.17530519061933 37.0 37.0 37.0 35.0 37.0 6 36.22972018553338 37.0 37.0 37.0 35.0 37.0 7 36.202945898605286 37.0 37.0 37.0 35.0 37.0 8 36.194964170507376 37.0 37.0 37.0 35.0 37.0 9 37.890427219961055 39.0 38.0 39.0 35.0 39.0 10-11 37.862820853877054 39.0 38.0 39.0 35.0 39.0 12-13 37.78715240969588 39.0 38.0 39.0 35.0 39.0 14-15 39.04143543119357 40.0 38.0 41.0 36.0 41.0 16-17 38.959957342332146 40.0 38.0 41.0 36.0 41.0 18-19 39.205528744674496 40.5 39.0 41.0 36.0 41.0 20-21 39.1757591738109 40.0 39.0 41.0 36.0 41.0 22-23 39.07668513200022 40.0 39.0 41.0 35.5 41.0 24-25 38.968842848776355 40.0 38.0 41.0 35.0 41.0 26-27 38.80936960999661 40.0 38.0 41.0 35.0 41.0 28-29 38.68643615488633 40.0 38.0 41.0 34.5 41.0 30-31 38.43108300621974 40.0 38.0 41.0 34.0 41.0 32-33 38.19340236781551 40.0 38.0 41.0 34.0 41.0 34-35 38.08914487659028 40.0 38.0 41.0 34.0 41.0 36-37 38.02058570867614 40.0 38.0 41.0 33.5 41.0 38-39 37.819899507218835 40.0 37.0 41.0 33.0 41.0 40-41 37.58779264326101 40.0 37.0 41.0 33.0 41.0 42-43 37.32304103740017 40.0 37.0 41.0 32.0 41.0 44-45 37.04713283648698 40.0 36.0 41.0 31.0 41.0 46-47 36.66941044503085 39.5 36.0 41.0 30.5 41.0 48-49 36.23690149603377 39.0 35.0 41.0 30.0 41.0 50-51 35.84286402081019 39.0 35.0 40.0 28.5 41.0 52-53 35.30550606226706 38.0 35.0 40.0 27.0 41.0 54-55 34.92248178371504 38.0 34.0 40.0 26.0 41.0 56-57 34.53990026441589 38.0 34.0 40.0 26.0 41.0 58-59 34.03142451549439 37.5 33.5 40.0 23.5 41.0 60-61 33.5490263316952 37.0 33.0 39.5 22.5 41.0 62-63 32.99741983427435 36.0 32.0 39.0 21.5 40.5 64-65 32.44088334742747 35.5 31.5 39.0 19.0 40.0 66-67 31.883019085386366 35.0 31.0 38.0 16.5 40.0 68-69 31.270975028579166 35.0 30.5 38.0 10.5 40.0 70-71 30.65193946310038 34.5 29.5 37.0 7.5 39.5 72-73 29.99464735389782 34.0 29.0 36.5 4.5 39.0 74-75 29.35672407739224 34.0 28.0 36.0 2.0 39.0 76-77 28.073496025720576 32.5 26.5 34.5 2.0 36.5 78-79 28.730744914383124 33.5 28.0 35.0 2.0 37.0 80-81 28.470271304375792 34.0 28.0 35.0 2.0 37.0 82-83 27.924929439955612 33.0 26.5 35.0 2.0 36.0 84-85 27.274371861485207 33.0 26.0 35.0 2.0 36.0 86-87 26.583587351726745 32.5 24.5 35.0 2.0 35.5 88-89 25.78625968776678 32.0 22.0 34.5 2.0 35.0 90-91 25.135055476410965 31.5 19.5 34.0 2.0 35.0 92-93 24.220618241346173 31.0 12.5 34.0 2.0 35.0 94-95 23.333224835259383 31.0 2.0 34.0 2.0 35.0 96-97 22.296414001846756 30.0 2.0 34.0 2.0 35.0 98-99 20.87987688511913 29.5 2.0 34.0 2.0 35.0 100-101 16.99407040994515 22.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1312157447714526 1101 2 0.1215555283423626 1101 3 0.11155772235865413 1101 4 0.053637288858972454 1101 5 0.003369830951335473 1101 6 0.006742777515484022 1101 7 0.15393718667484535 1101 8 0.1315398418970659 1101 9 0.0706878903082 1101 10-11 0.159076777196816 1101 12-13 0.19352827260334493 1101 14-15 0.3663487978158102 1101 16-17 0.3222180971391495 1101 18-19 0.2778438784664701 1101 20-21 0.21654785739544735 1101 22-23 0.22499199877442777 1101 24-25 0.26893556884146363 1101 26-27 0.28903092925973084 1101 28-29 0.3312205298248756 1101 30-31 0.3367682951550037 1101 32-33 0.43569736121705915 1101 34-35 0.30870695653020874 1101 36-37 0.3241800119301317 1101 38-39 0.37960448681518244 1101 40-41 0.4393429147389938 1101 42-43 0.49294197631977 1101 44-45 0.6367939296239769 1101 46-47 0.7647467947686195 1101 48-49 0.6553462729921407 1101 50-51 0.6942146772908799 1101 52-53 0.7635886091810491 1101 54-55 0.8899249151496704 1101 56-57 0.9260305128670296 1101 58-59 1.0751988860384785 1101 60-61 1.1073865359867625 1101 62-63 1.1599103441286474 1101 64-65 1.313843549078797 1101 66-67 1.3409238486510517 1101 68-69 1.671691121465713 1101 70-71 1.730060273560035 1101 72-73 1.7905709561729033 1101 74-75 1.9771650094907791 1101 76-77 1.9227114981314486 1101 78-79 2.0057022642605524 1101 80-81 2.165691179189956 1101 82-83 2.040523540309433 1101 84-85 1.98639675353769 1101 86-87 2.1932584780524564 1101 88-89 2.5858010774700126 1101 90-91 2.8187129283502372 1101 92-93 3.093526146913476 1101 94-95 3.4012090348700674 1101 96-97 3.8350431856121148 1101 98-99 4.0396243880918945 1101 100-101 3.902921826836362 1102 1 0.19691190162476602 1102 2 0.19071412580431968 1102 3 0.17485747577178046 1102 4 0.11242510385866922 1102 5 -0.024604548070733756 1102 6 0.07131425763817845 1102 7 0.1042622603501897 1102 8 0.2030319932172091 1102 9 0.18580847730525107 1102 10-11 0.225885582152614 1102 12-13 0.2840012984047533 1102 14-15 0.38201593560373936 1102 16-17 0.3321916341572475 1102 18-19 0.37347763681133017 1102 20-21 0.3088453253692265 1102 22-23 0.29930305909800126 1102 24-25 0.330704228250859 1102 26-27 0.4497248307452608 1102 28-29 0.42023598661203465 1102 30-31 0.41612731963326866 1102 32-33 0.29385562789173747 1102 34-35 0.3563634790538188 1102 36-37 0.421301861933145 1102 38-39 0.5713642751113284 1102 40-41 0.5547512866641782 1102 42-43 0.5850015781721751 1102 44-45 0.5122050079904881 1102 46-47 0.6044293893437143 1102 48-49 0.7351620846238234 1102 50-51 0.7116405508700012 1102 52-53 0.8120351118574192 1102 54-55 0.9494182091894814 1102 56-57 1.0407058902652366 1102 58-59 1.1179475779251646 1102 60-61 1.025836947967278 1102 62-63 1.1371093277095738 1102 64-65 1.2020774457528844 1102 66-67 1.4015446762206665 1102 68-69 1.5822433858549516 1102 70-71 1.5274142760860485 1102 72-73 1.739563408208749 1102 74-75 1.937637884934336 1102 76-77 1.8282657171727656 1102 78-79 1.9017421391628169 1102 80-81 2.1790228084676357 1102 82-83 2.086986522206349 1102 84-85 2.3097623636898525 1102 86-87 2.3807472012135804 1102 88-89 2.716909966626204 1102 90-91 2.9363644890647365 1102 92-93 2.9836936455602547 1102 94-95 3.354171385264003 1102 96-97 3.5233805284881576 1102 98-99 3.861507319468572 1102 100-101 3.5553099605947054 1103 1 0.20746998228755587 1103 2 0.18066292046515997 1103 3 0.19004906428797597 1103 4 0.09465311145316946 1103 5 -0.01508898509967338 1103 6 0.05762747321841033 1103 7 0.08480624516119661 1103 8 0.11583332800807256 1103 9 0.21751954111055483 1103 10-11 0.22617382793823992 1103 12-13 0.21452111889838932 1103 14-15 0.2990090602801203 1103 16-17 0.3563565268561746 1103 18-19 0.24620892384890425 1103 20-21 0.24757784938970673 1103 22-23 0.22491378345218038 1103 24-25 0.2701592365932939 1103 26-27 0.27576634159044744 1103 28-29 0.37037234240283823 1103 30-31 0.36758250057797426 1103 32-33 0.37628514863278184 1103 34-35 0.3658908365131097 1103 36-37 0.4306635212162675 1103 38-39 0.39062553740924244 1103 40-41 0.5250818200051768 1103 42-43 0.5671501887239287 1103 44-45 0.635004041417794 1103 46-47 0.7152424898557612 1103 48-49 0.8849064894336678 1103 50-51 0.9126376166314643 1103 52-53 1.0359687175187702 1103 54-55 1.202922898217743 1103 56-57 1.1455259524020533 1103 58-59 1.4274178328011757 1103 60-61 1.5443068288717186 1103 62-63 1.5482817286071082 1103 64-65 1.6889311940987781 1103 66-67 1.702903775236834 1103 68-69 1.730623369745416 1103 70-71 1.8865670866262931 1103 72-73 2.1156958229144642 1103 74-75 2.1596419410876067 1103 76-77 2.087661033897927 1103 78-79 1.9143786387888433 1103 80-81 2.3680081948090432 1103 82-83 2.4553847731700422 1103 84-85 2.651051702623171 1103 86-87 2.8546770671591517 1103 88-89 3.078592694892702 1103 90-91 3.214220912745766 1103 92-93 3.41809356501253 1103 94-95 3.52351060625244 1103 96-97 3.7971878468242757 1103 98-99 4.276294011833798 1103 100-101 3.99622115672841 1104 1 0.241706856940894 1104 2 0.18575017914656655 1104 3 0.1786842805269231 1104 4 0.13572461108326195 1104 5 0.07041786346633216 1104 6 0.08478595785254583 1104 7 0.13187062918005665 1104 8 0.17898065548509123 1104 9 0.24962790434943116 1104 10-11 0.2825544760527734 1104 12-13 0.28821592019266973 1104 14-15 0.4469813438834862 1104 16-17 0.3447125840723331 1104 18-19 0.329317373440972 1104 20-21 0.34119282947569474 1104 22-23 0.29488923911414844 1104 24-25 0.35135715567088255 1104 26-27 0.4557264594097603 1104 28-29 0.4390332805233328 1104 30-31 0.4832090173419985 1104 32-33 0.48846829806630154 1104 34-35 0.534957120302181 1104 36-37 0.5340704523727382 1104 38-39 0.5882542219361326 1104 40-41 0.6590675259804186 1104 42-43 0.6887287142283043 1104 44-45 0.8189682809101129 1104 46-47 0.99517544296436 1104 48-49 1.0873932853810757 1104 50-51 1.241213200442651 1104 52-53 1.315990121740768 1104 54-55 1.4670574808841295 1104 56-57 1.4516408411268955 1104 58-59 1.5449527144824202 1104 60-61 1.5208180164825507 1104 62-63 1.769759526768567 1104 64-65 1.8380638317881264 1104 66-67 1.9738832017920913 1104 68-69 2.025135935758911 1104 70-71 2.0236437089767207 1104 72-73 2.2294139747612682 1104 74-75 2.2596568024988564 1104 76-77 2.328006801278672 1104 78-79 2.3786169129594157 1104 80-81 2.4921716990269474 1104 82-83 2.5374408778398596 1104 84-85 2.8938418202448766 1104 86-87 2.9408024644447366 1104 88-89 3.1968952808053324 1104 90-91 3.609311327785189 1104 92-93 3.855368664352536 1104 94-95 3.87839853524034 1104 96-97 4.157611854527303 1104 98-99 4.4713333864526135 1104 100-101 4.1205198077508065 1105 1 0.14745536519107105 1105 2 0.1604721358083765 1105 3 0.12507580752789238 1105 4 0.07688164029236333 1105 5 0.028731939694573327 1105 6 -0.04004575317701864 1105 7 0.050050417711283046 1105 8 0.03365251505038458 1105 9 0.08298165562078452 1105 10-11 0.11571524282585699 1105 12-13 0.18722625446802255 1105 14-15 0.2748207889226606 1105 16-17 0.24721394049766587 1105 18-19 0.1915555738128205 1105 20-21 0.22029185774722038 1105 22-23 0.23981678087709923 1105 24-25 0.30757289122232834 1105 26-27 0.16211059524439264 1105 28-29 0.21482027754078814 1105 30-31 0.21932084403504604 1105 32-33 0.3539850722610822 1105 34-35 0.3833223813114941 1105 36-37 0.3486785011111877 1105 38-39 0.3754213252667924 1105 40-41 0.4399892209618699 1105 42-43 0.43524154031899087 1105 44-45 0.5144342901319021 1105 46-47 0.6160452471238926 1105 48-49 0.7397555008736489 1105 50-51 0.744864830983559 1105 52-53 0.9470330840082894 1105 54-55 1.007733066564093 1105 56-57 1.0197267771112521 1105 58-59 1.0428620702869296 1105 60-61 1.0677154122095374 1105 62-63 1.1997411379028904 1105 64-65 1.2069152178226403 1105 66-67 1.257483908341797 1105 68-69 1.4335172130822116 1105 70-71 1.582165526593009 1105 72-73 1.782638641411399 1105 74-75 1.6914830248559234 1105 76-77 1.641081723661884 1105 78-79 1.7105558474229206 1105 80-81 1.8098711253528599 1105 82-83 1.9633972292497468 1105 84-85 2.2471626611077262 1105 86-87 2.396087879867089 1105 88-89 2.220962623108562 1105 90-91 2.4565662600529734 1105 92-93 2.6464467887264043 1105 94-95 2.9853793073945987 1105 96-97 3.292498414117471 1105 98-99 3.2545757931741264 1105 100-101 3.09835680390956 1106 1 0.0667668436545128 1106 2 0.08341358051340819 1106 3 0.06876954099492139 1106 4 0.07154125611659623 1106 5 -0.09748241349505804 1106 6 -0.007642277911614315 1106 7 -0.011433912025260895 1106 8 0.06650279633013412 1106 9 0.09955351409915636 1106 10-11 0.08741557315266135 1106 12-13 0.03210522132285121 1106 14-15 -0.03908801378478444 1106 16-17 -0.062379809540139775 1106 18-19 -0.07961503617293886 1106 20-21 -0.17491029959768412 1106 22-23 -0.021094526798549396 1106 24-25 -0.002979000083094263 1106 26-27 -0.17446133580669 1106 28-29 -0.01097635919769857 1106 30-31 0.11195465300189511 1106 32-33 0.18273632565234266 1106 34-35 0.22654215447337123 1106 36-37 0.2315133606890285 1106 38-39 0.08019322317634447 1106 40-41 0.07210186107256078 1106 42-43 0.23048439886147065 1106 44-45 0.3199121391250088 1106 46-47 0.46704427102087465 1106 48-49 0.5942767810079559 1106 50-51 0.7255941746722101 1106 52-53 0.7081974285496742 1106 54-55 0.8420480466610272 1106 56-57 0.8760623146392064 1106 58-59 0.8926456826421969 1106 60-61 0.891101122883903 1106 62-63 0.7898192382395095 1106 64-65 0.9266973303418027 1106 66-67 1.0757149605131104 1106 68-69 1.2435268047760246 1106 70-71 1.0626472158922837 1106 72-73 1.4363561182516307 1106 74-75 1.692178755282157 1106 76-77 1.6803595283856403 1106 78-79 1.6834042176211774 1106 80-81 1.4256660929189025 1106 82-83 1.7805499718331141 1106 84-85 1.9982212551081382 1106 86-87 2.0800099996677055 1106 88-89 2.144876905055977 1106 90-91 2.2085150793553794 1106 92-93 2.2938633210327524 1106 94-95 2.4599162632353107 1106 96-97 2.9461321299493264 1106 98-99 3.230286370768269 1106 100-101 2.9044521862286707 1107 1 0.21294361364533643 1107 2 0.18647692846127484 1107 3 0.16996996773663398 1107 4 0.1360452024230625 1107 5 0.0629741963046655 1107 6 0.10750345840954623 1107 7 0.14512791280358783 1107 8 0.1195040309288018 1107 9 0.12842471900227537 1107 10-11 0.20026848337394654 1107 12-13 0.22424503099527726 1107 14-15 0.363800808112849 1107 16-17 0.2943745139515528 1107 18-19 0.22652241932686934 1107 20-21 0.17710009288582995 1107 22-23 0.3058179508914449 1107 24-25 0.316371680575692 1107 26-27 0.31684878617702594 1107 28-29 0.31828130480359107 1107 30-31 0.39405961018393754 1107 32-33 0.36762143033442385 1107 34-35 0.3570952125014273 1107 36-37 0.4080047760107064 1107 38-39 0.3871045793753112 1107 40-41 0.46572568135027126 1107 42-43 0.5506540315683637 1107 44-45 0.5657517189397083 1107 46-47 0.5090088836655511 1107 48-49 0.6316994748102331 1107 50-51 0.7105338442061893 1107 52-53 0.7922456325345166 1107 54-55 0.7508093598215808 1107 56-57 0.7413738782660957 1107 58-59 0.7458948674044876 1107 60-61 0.7974889728226486 1107 62-63 0.8299366257597143 1107 64-65 0.8002074007561006 1107 66-67 1.1109848947764789 1107 68-69 1.3750326509074569 1107 70-71 1.4727715719934835 1107 72-73 1.6219348459631924 1107 74-75 1.4860774606789242 1107 76-77 1.4316190490776322 1107 78-79 1.4587128912519525 1107 80-81 1.594179225866391 1107 82-83 1.7954925217778062 1107 84-85 1.739420066711066 1107 86-87 1.9888198388823177 1107 88-89 2.0870304937314117 1107 90-91 2.17645876804481 1107 92-93 2.422869775836663 1107 94-95 2.51038683416051 1107 96-97 2.796482092662149 1107 98-99 3.114636203119737 1107 100-101 2.956631473343794 1108 1 0.1797224244352833 1108 2 0.1693965544867524 1108 3 0.1130864690007698 1108 4 0.04858283501640415 1108 5 0.007192543726354472 1108 6 0.07797870430262321 1108 7 0.10696122804915831 1108 8 0.10883174901696435 1108 9 0.08969681119358341 1108 10-11 0.07877047503750134 1108 12-13 0.12270259710605558 1108 14-15 0.2873375584253708 1108 16-17 0.14543561240122926 1108 18-19 0.20127567210936803 1108 20-21 0.24134782287172385 1108 22-23 0.249803669598613 1108 24-25 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-0.306661078246826 2316 50-51 -0.2502051732382924 2316 52-53 -0.012887535327799071 2316 54-55 0.2447039848663266 2316 56-57 0.12946502219475775 2316 58-59 0.27954727710866223 2316 60-61 0.37041150748597573 2316 62-63 0.5296372606704693 2316 64-65 0.7251371793154178 2316 66-67 0.8652565369855978 2316 68-69 1.1020747137449405 2316 70-71 1.1953107092618076 2316 72-73 1.2959031159870058 2316 74-75 1.584218245914137 2316 76-77 1.7335300059566094 2316 78-79 1.7695633682130953 2316 80-81 1.8582277934268099 2316 82-83 2.037560222249322 2316 84-85 2.2968057672137014 2316 86-87 2.313450252352382 2316 88-89 2.6300717629304593 2316 90-91 2.878234923099974 2316 92-93 2.819065368809074 2316 94-95 3.26678204276293 2316 96-97 3.681358611857302 2316 98-99 4.116987551527011 2316 100-101 4.443123669232435 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 7.0 4 18.0 5 49.0 6 109.0 7 304.0 8 705.0 9 1430.0 10 2554.0 11 4242.0 12 6648.0 13 9681.0 14 13095.0 15 18298.0 16 23649.0 17 33428.0 18 34097.0 19 28132.0 20 26337.0 21 27660.0 22 29637.0 23 32569.0 24 37173.0 25 41597.0 26 46790.0 27 54537.0 28 65932.0 29 79291.0 30 95243.0 31 115851.0 32 140538.0 33 176692.0 34 232316.0 35 319600.0 36 445039.0 37 540081.0 38 288090.0 39 13269.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.87031961756858 18.82810688674074 15.25879387219854 23.04277962349214 2 15.628931049434799 19.89384951817104 39.84761350742932 24.62960592496483 3 19.253170006851803 23.482827768405638 30.304813183609948 26.959189041132607 4 13.058058748951296 16.976699304972943 36.73370787420359 33.231534071872176 5 13.58720919701242 37.167904111914154 34.48322823937941 14.761658451694014 6 32.37709778905012 36.40270848898881 16.771993071307236 14.448200650653837 7 28.417035995673928 33.06719755338239 20.18677304056834 18.32899341037534 8 26.691471112481068 34.56082394092499 19.416922417143546 19.330782529450392 9 25.99182763246593 16.87708480473027 20.24336179411477 36.88772576868903 10-11 25.068834573215593 26.440349624014807 26.88257280818255 21.608242994587044 12-13 26.14611490900904 23.71797492002525 27.42584494480503 22.71006522616069 14-15 22.567323959611908 25.233746938335166 26.00937148780703 26.189557614245896 16-17 21.94016431519449 28.358501492868353 27.100716137904417 22.600618054032736 18-19 22.1021130488506 26.413294906141072 29.68758585475486 21.79700619025347 20-21 22.314580532932712 25.642913908704816 30.155557089307706 21.88694846905476 22-23 22.20409262756124 25.56743025392303 30.138031362784133 22.090445755731594 24-25 22.119847319932088 25.843153645835514 30.065526452232195 21.971472582000203 26-27 21.53244097110651 26.23019232953584 29.843813714331286 22.393552985026368 28-29 21.794560376749093 26.781942659410085 29.564708854380957 21.85878810945987 30-31 21.900400443328827 26.146098156861747 29.454060921528924 22.499440478280505 32-33 21.473622738962682 26.202753918997335 29.495338212452072 22.82828512958791 34-35 22.448671181423563 26.372140238372598 29.173426208587667 22.00576237161617 36-37 22.57959247126905 25.745868977941637 29.345208001010498 22.32933054977882 38-39 21.939126048319665 26.24079885313297 29.151290585608177 22.668784512939194 40-41 22.536876032664622 26.070293738320483 29.22907889750371 22.163751331511186 42-43 22.155138878604316 26.165240359147372 28.990140099909095 22.689480662339214 44-45 22.169976003371993 26.36069123154769 29.284396562873376 22.18493620220694 46-47 22.79831935729371 26.736063006846774 28.300596560817088 22.16502107504243 48-49 22.364451675415754 26.80822677850847 28.186995509084355 22.64032603699142 50-51 22.52617690535573 27.074853954779925 28.199040302986038 22.19992883687831 52-53 23.005891395159033 26.64479282954489 28.161548997350483 22.187766777945598 54-55 22.27960204939069 26.968879871021866 28.352004159893212 22.39951391969423 56-57 22.48369345982768 27.01091100857308 28.117574610713604 22.38782092088564 58-59 22.53887503300173 26.563812949543873 27.932882186838707 22.96442983061569 60-61 21.980693485290946 26.98868090911893 28.396045555119255 22.63458005047087 62-63 22.16778146622834 26.967154399850973 28.510931781235723 22.354132352684967 64-65 21.82592039647642 27.053846762077967 28.23400203438077 22.88623080706485 66-67 21.694832629184273 27.106181881535434 28.193631499418697 23.005353989861593 68-69 22.52464010222916 26.612282040173994 28.333897885425745 22.5291799721711 70-71 22.193211225814924 26.934571473371456 27.797993896857697 23.07422340395592 72-73 22.21450692642774 27.059865316063654 27.87063587643815 22.854991881070454 74-75 22.227352101992434 27.546158866643527 27.540580401595875 22.685908629768164 76-77 22.307728904691004 26.91678069294922 28.01416360549095 22.761326796868822 78-79 22.379109804294714 27.272780575014107 27.778025337287733 22.570084283403446 80-81 22.660248796607483 27.34569822564309 27.395854238652163 22.598198739097267 82-83 22.515088510719135 27.46012624309443 27.574461697790202 22.450323548396227 84-85 22.428123387019898 27.689121052852894 27.51117285819278 22.37158270193443 86-87 22.727543053847484 27.630938159163748 27.037827947445653 22.603690839543116 88-89 22.387636647265445 27.867950863941743 26.748639390597084 22.995773098195727 90-91 22.31177387149426 27.76191218886549 27.09293288948029 22.83338105015996 92-93 22.530013147085192 27.335684888088956 27.292313578754523 22.84198838607133 94-95 22.20112195850057 27.146691710014252 27.47355966269881 23.17862666878637 96-97 22.587086096043475 26.672485249724424 27.504698980463633 23.235729673768464 98-99 22.27110871071454 27.158698451967574 26.937737629209312 23.632455208108574 100-101 22.547703414623687 26.572423553251056 27.30022059227552 23.579652439849742 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 707.0 1 505.0 2 444.5 3 597.0 4 484.0 5 455.5 6 940.0 7 2272.0 8 3651.0 9 4151.0 10 3512.0 11 2662.5 12 2600.0 13 2744.0 14 2997.0 15 3264.0 16 3512.0 17 3864.5 18 4272.0 19 4753.5 20 5431.0 21 6529.0 22 8154.0 23 10136.0 24 12679.5 25 16132.5 26 20692.5 27 25870.5 28 30238.5 29 33833.5 30 37863.0 31 44706.5 32 55065.5 33 58711.0 34 56778.0 35 58677.5 36 63210.0 37 68399.5 38 73291.5 39 76545.0 40 80036.0 41 86118.0 42 90413.5 43 92921.5 44 95883.5 45 102104.0 46 105870.5 47 108062.5 48 110655.0 49 107544.0 50 104136.0 51 101685.5 52 99601.5 53 102089.0 54 105691.5 55 100790.0 56 92263.0 57 84688.5 58 79135.5 59 70243.5 60 55401.5 61 46097.0 62 40325.0 63 30287.0 64 20721.0 65 15499.0 66 11710.5 67 9001.0 68 6844.5 69 5122.0 70 3785.0 71 2617.0 72 1820.0 73 1371.5 74 986.5 75 728.5 76 553.0 77 370.0 78 229.0 79 125.5 80 72.5 81 43.0 82 24.0 83 15.0 84 9.5 85 7.0 86 5.5 87 3.0 88 0.5 89 0.5 90 1.0 91 2.5 92 1.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014674881026249944 3 0.002579830682696908 4 0.0020102576748287594 5 0.11894024576070161 6 6.700858916095866E-5 7 0.0 8 4.020515349657519E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.6752147290239665E-5 16-17 5.0256441870718985E-5 18-19 0.0 20-21 0.0 22-23 2.6803435664383464E-4 24-25 6.533337443193468E-4 26-27 0.005997268729905799 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011073169358848417 36-37 6.365815970291072E-4 38-39 0.007153166892932336 40-41 0.010084792668724278 42-43 0.005997268729905799 44-45 0.0035514552255308087 46-47 1.6752147290239665E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 6.700858916095866E-5 68-69 8.376073645119831E-4 70-71 0.0 72-73 1.6752147290239665E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.6752147290239662E-4 82-83 0.0017924797600556439 84-85 0.0038697460240453624 86-87 0.0028981214812114618 88-89 0.0 90-91 0.0023620527679237926 92-93 0.0 94-95 1.6752147290239665E-5 96-97 6.700858916095866E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2984692.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.4784415460076 #Duplication Level Percentage of deduplicated Percentage of total 1 70.8836454605409 33.654450175662184 2 15.352267582071894 14.578034779881358 3 5.64250397752934 8.036918858107263 4 2.7158451099896803 5.157763732106225 5 1.5122276877458458 3.589910693844769 6 0.911979885010452 2.597963019696209 7 0.5915898675570754 1.9661435462213286 8 0.4133378105974239 1.569970806336364 9 0.3116352848970708 1.3316361891893103 >10 1.5069233214031112 12.659725599924782 >50 0.09795542106095932 3.215968582739571 >100 0.05084406202266136 4.6083732312582875 >500 0.00451641139490186 1.3961209805287769 >1k 0.004375273538482691 4.250950332234153 >5k 2.1170678412013022E-4 0.5939436180454369 >10k+ 1.411378560800868E-4 0.7921258542240776 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12740 0.4268447129553066 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10902 0.3652638195163856 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6943 0.23262031727226798 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5490 0.1839385772468315 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5294 0.17737173550905755 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4914 0.1646401035684754 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4828 0.1617587342345542 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4635 0.1552924053805217 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3845 0.12882401266194302 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3740 0.12530606173099268 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3619 0.12125204208675469 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3541 0.1186387071094773 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3249 0.10885545309197732 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3013 0.1009484395709842 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.350429458047932E-5 2 0.0 0.0 0.0 0.0 3.350429458047932E-5 3 0.0 0.0 0.0 0.0 3.350429458047932E-5 4 0.0 0.0 0.0 0.0 3.350429458047932E-5 5 0.0 0.0 0.0 0.0 1.0051288374143798E-4 6 0.0 0.0 0.0 0.0 1.6752147290239664E-4 7 0.0 0.0 0.0 0.0 1.6752147290239664E-4 8 0.0 0.0 0.0 0.0 1.6752147290239664E-4 9 0.0 0.0 0.0 0.0 1.6752147290239664E-4 10-11 0.0 0.0 0.0 0.0 1.6752147290239664E-4 12-13 0.0 0.0 0.0 0.0 4.5230797683647086E-4 14-15 0.0 0.0 0.0 0.0 5.863251551583882E-4 16-17 0.0 0.0 0.0 3.350429458047932E-5 6.533337443193469E-4 18-19 0.0 0.0 0.0 5.0256441870718985E-5 7.035901861900659E-4 20-21 0.0 0.0 0.0 8.376073645119831E-5 9.213681009631815E-4 22-23 0.0 0.0 0.0 1.5076932561215695E-4 0.0010721374265753383 24-25 0.0 0.0 0.0 3.5179509309503295E-4 0.0010888895738655779 26-27 0.0 0.0 0.0 0.0010553852792850988 0.0011223938684460574 28-29 0.0 0.0 0.0 0.0034006858999186514 0.001139146015736297 30-31 0.0 0.0 0.0 0.010587357087431467 0.0011558981630265365 32-33 0.0 0.0 0.0 0.027121726462898014 0.0012061546048972556 34-35 0.0 0.0 0.0 0.051244818560843126 0.001239658899477735 36-37 0.0 0.0 0.0 0.08898740640575309 0.001239658899477735 38-39 0.0 0.0 0.0 0.14840727284423316 0.0013066674886386938 40-41 0.0 0.0 0.0 0.21112731229889048 0.0013401717832191731 42-43 0.0 0.0 0.0 0.27629316525792275 0.0013401717832191731 44-45 0.0 0.0 0.0 0.3581274047707435 0.001390428225089892 46-47 0.0 0.0 0.0 0.45338011426304625 0.0014741889615410904 48-49 0.0 0.0 0.0 0.5497384654765047 0.001541197550702049 50-51 0.0 0.0 0.0 0.6542216081257295 0.001641710434443487 52-53 0.0 0.0 0.0 0.7510490194633148 0.0017087190236044457 54-55 0.0 0.0 0.0 0.8558001964691835 0.0017087190236044457 56-57 0.0 0.0 0.0 0.9794645477657327 0.0017589754654751648 58-59 0.0 0.0 0.0 1.1099637751566998 0.0018259840546361234 60-61 0.0 0.0 0.0 1.2511341203715491 0.001842736201926363 62-63 0.0 0.0 0.0 1.389523609136219 0.0019097447910873216 64-65 0.0 0.0 0.0 1.5261206181408333 0.0019600012329580403 66-67 0.0 0.0 0.0 1.6889179855073824 0.0020270098221189994 68-69 0.0 0.0 0.0 1.867830918567142 0.002043761969409239 70-71 0.0 0.0 0.0 2.0555052246596968 0.0020605141166994785 72-73 0.0 0.0 0.0 2.250600732001828 0.0021107705585701976 74-75 0.0 0.0 0.0 2.4478572663444 0.0021610270004409167 76-77 0.0 0.0 0.0 2.6579291933640055 0.002177779147731156 78-79 0.0 0.0 0.0 2.8994281487001006 0.0021945312950213958 80-81 0.0 0.0 0.0 3.143205396067668 0.002228035589601875 82-83 0.0 0.0 0.0 3.3975532483753765 0.002278292031472594 84-85 0.0 0.0 0.0 3.6595065755528546 0.002278292031472594 86-87 0.0 0.0 0.0 3.920404517451047 0.002328548473343313 88-89 0.0 0.0 0.0 4.196831699887292 0.0024123092097945113 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7605 0.0 44.277157 1 GTATCAA 12655 0.0 37.732643 1 ATCAACG 15845 0.0 29.890461 3 TCAACGC 15930 0.0 29.759323 4 TATCAAC 15975 0.0 29.652693 2 CAACGCA 16280 0.0 29.031427 5 AACGCAG 16815 0.0 28.13221 6 GTGTAAG 1290 0.0 27.651407 1 TAAGGTG 2620 0.0 27.040913 5 AGGTAAG 2565 0.0 25.21722 2 ACGCAGA 18740 0.0 25.141052 7 CGCAGAG 18720 0.0 25.117172 8 AAGGTAA 2500 0.0 24.92166 1 GTAAGGT 2920 0.0 24.100304 4 GGTAAGG 2815 0.0 23.818052 3 GCAGAGT 20455 0.0 22.66166 9 GAGTACT 13080 0.0 22.512583 12-13 TAAGGTA 1545 0.0 22.466637 4 AGAGTAC 17330 0.0 21.636894 10-11 GTACTTT 13530 0.0 21.55321 14-15 >>END_MODULE