FastQCFastQC Report
Thu 26 May 2016
SRR938238_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938238_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2961283
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134090.4528104878865006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109400.36943446472356745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68700.23199403771946145No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA53370.18022593585280436No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA48640.1642531294712461No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA48400.16344266995082873No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC48340.16324005507072442No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA44670.15084677823767603No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG37570.12687068409199662No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA37560.12683691494531255No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA36020.121636466355968No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA32930.11120180003059484No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT31750.1072170407218763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75350.047.346361
GTATCAA129300.039.0783651
TCAACGC158000.031.5255934
TATCAAC158800.031.4859642
ATCAACG158250.031.4757883
CAACGCA159950.031.111545
TAAGGTG20800.030.6196755
AACGCAG165600.030.0167966
GTAAGGT23050.027.8369674
ACGCAGA183100.026.8014437
CGCAGAG183700.026.5582948
GGTAAGG23100.025.9249323
AGGTAAG23150.024.8419762
GCAGAGT199700.023.7877569
AAGGTAA23600.023.474441
GAGTACT133300.023.37847712-13
GTGTAAG13300.022.2632881
GTACTTT139550.022.19676414-15
GTACATG89650.022.161151
AAGGTGT31800.021.5193126
Produced by FastQC (version 0.11.5)