##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938238_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2961283 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.898111055241934 33.0 31.0 34.0 30.0 34.0 2 32.09128036732727 34.0 31.0 34.0 30.0 34.0 3 32.17209668917155 34.0 31.0 34.0 30.0 34.0 4 35.69587945495247 37.0 35.0 37.0 35.0 37.0 5 35.6996909785387 37.0 35.0 37.0 35.0 37.0 6 35.72748535009994 37.0 35.0 37.0 35.0 37.0 7 35.67286814532755 37.0 35.0 37.0 33.0 37.0 8 35.64487588656674 37.0 35.0 37.0 33.0 37.0 9 37.290540958091476 39.0 37.0 39.0 34.0 39.0 10-11 37.20431363702828 39.0 37.0 39.0 34.0 39.0 12-13 37.10427490381703 39.0 37.0 39.0 33.5 39.0 14-15 38.29442677380041 40.0 38.0 41.0 33.5 41.0 16-17 38.14419881517571 40.0 38.0 41.0 33.0 41.0 18-19 38.2995259149497 40.0 38.0 41.0 33.5 41.0 20-21 38.27605196801521 40.0 38.0 41.0 34.0 41.0 22-23 38.1898705729915 40.0 38.0 41.0 33.5 41.0 24-25 38.078050797576594 40.0 38.0 41.0 33.0 41.0 26-27 37.898992092278924 40.0 38.0 41.0 33.0 41.0 28-29 37.68941046836793 40.0 38.0 41.0 32.5 41.0 30-31 37.45607360053058 40.0 37.0 41.0 32.0 41.0 32-33 37.18180987767802 40.0 36.5 41.0 31.0 41.0 34-35 36.93401339892202 40.0 36.0 41.0 31.0 41.0 36-37 36.65421474408221 39.0 36.0 41.0 30.0 41.0 38-39 36.34267393558805 39.0 35.0 40.5 30.0 41.0 40-41 36.10334692766615 39.0 35.0 40.0 29.5 41.0 42-43 35.90594549727263 39.0 35.0 40.0 28.5 41.0 44-45 35.61988182149426 39.0 35.0 40.0 27.0 41.0 46-47 35.217344306504984 38.5 35.0 40.0 25.5 41.0 48-49 34.79863120140831 38.0 34.0 40.0 24.0 41.0 50-51 34.07813741543784 37.5 33.5 39.5 23.5 40.5 52-53 34.19208869939145 38.0 34.0 40.0 23.0 40.5 54-55 34.28214645476302 38.0 34.0 40.0 23.0 41.0 56-57 33.959493908552474 38.0 34.0 40.0 22.0 41.0 58-59 33.61631833229043 37.5 33.0 40.0 20.5 41.0 60-61 33.174375600035525 37.0 33.0 40.0 18.5 41.0 62-63 32.62803048543486 36.0 32.0 39.0 14.5 41.0 64-65 32.09948812727456 36.0 31.5 39.0 9.0 40.5 66-67 31.551763036494656 35.0 31.0 39.0 7.0 40.0 68-69 30.936519913834644 35.0 30.0 38.0 2.0 40.0 70-71 30.359006383381796 35.0 29.5 37.5 2.0 39.5 72-73 29.704638833910842 34.0 29.0 37.0 2.0 39.0 74-75 29.010615500105864 34.0 28.0 36.0 2.0 39.0 76-77 28.339182374666656 34.0 26.5 35.5 2.0 38.5 78-79 27.603322951572004 33.0 26.0 35.0 2.0 37.0 80-81 26.92634628301314 33.0 25.0 35.0 2.0 37.0 82-83 26.17870615540629 32.0 23.0 35.0 2.0 36.0 84-85 25.506319051573254 32.0 20.0 35.0 2.0 36.0 86-87 24.811490830157062 31.0 17.5 34.0 2.0 35.0 88-89 23.97118698212903 31.0 9.0 34.0 2.0 35.0 90-91 23.11081598752973 30.0 2.0 34.0 2.0 35.0 92-93 22.197107132280166 29.5 2.0 34.0 2.0 35.0 94-95 21.22482957555897 29.0 2.0 34.0 2.0 35.0 96-97 19.99464083642124 28.0 2.0 34.0 2.0 35.0 98-99 18.465416679189392 25.5 2.0 33.0 2.0 35.0 100-101 14.825169023021441 9.5 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4164607199246788 1101 2 0.36696429639944483 1101 3 0.30513880170487795 1101 4 0.18210637056130707 1101 5 0.20984556244643215 1101 6 0.2568579285896959 1101 7 0.20937773743266774 1101 8 0.21368411222027106 1101 9 0.3248127983360263 1101 10-11 0.35424988570515126 1101 12-13 0.3644875994077523 1101 14-15 0.43423053411124357 1101 16-17 0.543472709708297 1101 18-19 0.49046531956287964 1101 20-21 0.5949926863921249 1101 22-23 0.5557154654575029 1101 24-25 0.5793388432255568 1101 26-27 0.6087096634494671 1101 28-29 0.5834939907983809 1101 30-31 0.5228474492590109 1101 32-33 0.46754406404861726 1101 34-35 0.6526514662513989 1101 36-37 0.722456113607727 1101 38-39 0.8553660048247664 1101 40-41 0.8590412918627663 1101 42-43 0.9928684366508307 1101 44-45 0.9139160049822479 1101 46-47 1.0340168398500467 1101 48-49 0.9525589420823479 1101 50-51 0.8978910664927113 1101 52-53 0.9462052038651834 1101 54-55 0.9502516457450696 1101 56-57 1.1239598169827119 1101 58-59 1.136521682289036 1101 60-61 1.1565016710111706 1101 62-63 1.273073560768772 1101 64-65 1.2278077062597603 1101 66-67 1.236766082921445 1101 68-69 1.5853838407096426 1101 70-71 1.9503859442978815 1101 72-73 2.119777402756217 1101 74-75 2.1860250708933258 1101 76-77 2.3018560907585446 1101 78-79 2.4938899778236525 1101 80-81 2.7142272425365768 1101 82-83 2.8518280968212615 1101 84-85 2.9396465949925137 1101 86-87 3.0443771310585532 1101 88-89 3.3805268649253506 1101 90-91 3.751135939627943 1101 92-93 3.96473008097707 1101 94-95 4.188574486565596 1101 96-97 4.58972464639503 1101 98-99 5.013431890888285 1101 100-101 4.8604927886850575 1102 1 0.32897979224073026 1102 2 0.26410885569056575 1102 3 0.17257169029494435 1102 4 0.1340770209161164 1102 5 0.22233591632738836 1102 6 0.217288743010414 1102 7 0.06348971117952118 1102 8 0.12349780509433828 1102 9 0.24649943933745533 1102 10-11 0.32265209591488997 1102 12-13 0.31944397905517974 1102 14-15 0.3929369567280574 1102 16-17 0.43136091947792465 1102 18-19 0.25063952217969643 1102 20-21 0.3703802160951284 1102 22-23 0.4076842481666745 1102 24-25 0.3208254241916393 1102 26-27 0.45916923961300427 1102 28-29 0.5112954795555638 1102 30-31 0.42684071923856237 1102 32-33 0.4333518708615145 1102 34-35 0.46118009712368746 1102 36-37 0.499928977934637 1102 38-39 0.47733483625513173 1102 40-41 0.6112765794842545 1102 42-43 0.7599934614512733 1102 44-45 0.817455355869825 1102 46-47 0.7941738547135273 1102 48-49 1.0287333731474035 1102 50-51 0.9462696523385006 1102 52-53 0.9032061141394294 1102 54-55 1.1032707625868738 1102 56-57 1.2394339783759918 1102 58-59 1.156560504957703 1102 60-61 1.1828463014352266 1102 62-63 1.212495459482291 1102 64-65 1.2762999072151828 1102 66-67 1.3149211657190527 1102 68-69 1.5619964512153892 1102 70-71 1.8340657258970232 1102 72-73 1.9930259097900702 1102 74-75 1.9813790858306994 1102 76-77 2.1507622729633447 1102 78-79 2.2904003766850956 1102 80-81 2.3214148767736056 1102 82-83 2.629522642565181 1102 84-85 2.6996262511887217 1102 86-87 2.773778623713426 1102 88-89 3.156515695321744 1102 90-91 3.451761343776983 1102 92-93 3.7530525454248114 1102 94-95 4.1175473816139245 1102 96-97 4.1841382611527855 1102 98-99 4.197204567557414 1102 100-101 4.261974278286736 1103 1 0.5640650905925 1103 2 0.4664800934148161 1103 3 0.4672763409027354 1103 4 0.34526838792228176 1103 5 0.3552261421729952 1103 6 0.4051988435772671 1103 7 0.4343627233866343 1103 8 0.37229236283366873 1103 9 0.5392769220096199 1103 10-11 0.5746687578342105 1103 12-13 0.6716188432424488 1103 14-15 0.802821166268437 1103 16-17 0.846269141633428 1103 18-19 0.8410241153573708 1103 20-21 0.8039719913734942 1103 22-23 0.8485946425560726 1103 24-25 0.8977137472476073 1103 26-27 0.8143974845557906 1103 28-29 0.9944066176110979 1103 30-31 0.9699662340695383 1103 32-33 1.0204123559638205 1103 34-35 1.0395790722090794 1103 36-37 1.218059977880749 1103 38-39 1.2749119550492551 1103 40-41 1.208657100204519 1103 42-43 1.327381629256017 1103 44-45 1.43702076153739 1103 46-47 1.4421630628296995 1103 48-49 1.3823749442896656 1103 50-51 1.3536417753795789 1103 52-53 1.327231976938414 1103 54-55 1.4619706684096911 1103 56-57 1.647819885415153 1103 58-59 1.6103849774243528 1103 60-61 1.6598688013133298 1103 62-63 1.7683931130077752 1103 64-65 1.9170999256913532 1103 66-67 2.126462232872253 1103 68-69 2.1832059479705173 1103 70-71 2.2746681839984397 1103 72-73 2.3459123954928423 1103 74-75 2.4589423988145818 1103 76-77 2.6226959652990693 1103 78-79 2.927849823811151 1103 80-81 3.095684105470191 1103 82-83 3.0960023341669896 1103 84-85 3.0240305548549635 1103 86-87 3.127189884173635 1103 88-89 3.3366484800152563 1103 90-91 3.7607483039582554 1103 92-93 3.7680148809675877 1103 94-95 4.178235208635847 1103 96-97 4.314449520586546 1103 98-99 4.710630108562981 1103 100-101 4.666902798575887 1104 1 0.25733194118111413 1104 2 0.24571825029015315 1104 3 0.24373677248943437 1104 4 0.2053782686879515 1104 5 0.21001086846312234 1104 6 0.2191290414193361 1104 7 0.24996640623464828 1104 8 0.2385989802445323 1104 9 0.2938025356601557 1104 10-11 0.2609340164272069 1104 12-13 0.2170804747308921 1104 14-15 0.44092088093746185 1104 16-17 0.4347176072708905 1104 18-19 0.4499116552753577 1104 20-21 0.38373888447831206 1104 22-23 0.38431947296366076 1104 24-25 0.47549714136336263 1104 26-27 0.4791938319415223 1104 28-29 0.6325571155230776 1104 30-31 0.5938354211597314 1104 32-33 0.5302790676661644 1104 34-35 0.4790714287405109 1104 36-37 0.5638525271522994 1104 38-39 0.7124556522583063 1104 40-41 0.7492494453982985 1104 42-43 0.8201621417179865 1104 44-45 0.7991844111282873 1104 46-47 0.7704170876969556 1104 48-49 1.081173625342558 1104 50-51 1.0327389201803143 1104 52-53 0.9431707110893228 1104 54-55 1.1132149759624568 1104 56-57 1.2048087225497497 1104 58-59 1.1899705861618983 1104 60-61 1.2531180549177634 1104 62-63 1.4360545801002402 1104 64-65 1.4038437277737046 1104 66-67 1.4771624511220942 1104 68-69 1.7067528386188364 1104 70-71 1.8220583977132243 1104 72-73 1.8206052159232975 1104 74-75 1.9059784289349473 1104 76-77 1.9017670098749448 1104 78-79 2.2709183835511375 1104 80-81 2.2581597767538035 1104 82-83 2.624410244070429 1104 84-85 2.778468183584579 1104 86-87 2.957076058849097 1104 88-89 3.1213127673492096 1104 90-91 3.191645891872316 1104 92-93 3.4752128742027857 1104 94-95 3.6721477150132777 1104 96-97 3.9847836034647983 1104 98-99 3.991897823647726 1104 100-101 4.122732596765644 1105 1 0.3787896516108873 1105 2 0.3637227530568339 1105 3 0.3012298781303073 1105 4 0.16001124230005814 1105 5 0.1983715023869408 1105 6 0.22917453347296401 1105 7 0.3214835492550918 1105 8 0.23966550723790903 1105 9 0.28571148725318096 1105 10-11 0.29918286260441107 1105 12-13 0.26828538008527403 1105 14-15 0.3711009826739158 1105 16-17 0.4553548041244895 1105 18-19 0.4158261044946485 1105 20-21 0.4539136591613655 1105 22-23 0.48010250515996233 1105 24-25 0.49263636633737207 1105 26-27 0.49344403466425746 1105 28-29 0.5584999102033521 1105 30-31 0.6420379983283269 1105 32-33 0.7181560471152579 1105 34-35 0.7114024008451452 1105 36-37 0.7724238587638368 1105 38-39 0.8767142050144372 1105 40-41 1.0073355820209144 1105 42-43 0.8916165478564722 1105 44-45 0.86600343241507 1105 46-47 1.0015195555560226 1105 48-49 1.0292754886056343 1105 50-51 1.042642863539406 1105 52-53 0.9318527319938781 1105 54-55 1.1190961679364193 1105 56-57 1.1351979100155987 1105 58-59 1.2784164172179473 1105 60-61 1.356516808753419 1105 62-63 1.3180824504577018 1105 64-65 1.3652294001880492 1105 66-67 1.505674131345291 1105 68-69 1.707961584095532 1105 70-71 1.8624962252399264 1105 72-73 2.0371690253603525 1105 74-75 2.201094842689372 1105 76-77 2.235578237499535 1105 78-79 2.381695530536735 1105 80-81 2.5121896725525303 1105 82-83 2.755436780208658 1105 84-85 2.8166471484662132 1105 86-87 3.03985871417758 1105 88-89 3.401269181960963 1105 90-91 3.5519439166205657 1105 92-93 3.682632838909381 1105 94-95 4.022854364497629 1105 96-97 4.1549193294622455 1105 98-99 4.19394641726991 1105 100-101 4.320624257984694 1106 1 0.14066126882637064 1106 2 0.16547805921883452 1106 3 0.057445989577701084 1106 4 0.11347776997978798 1106 5 0.08693735407268832 1106 6 -0.05407140321204196 1106 7 0.04477127915419743 1106 8 0.09559967122572033 1106 9 0.06517788866141672 1106 10-11 0.05663453616523384 1106 12-13 0.19914500636581067 1106 14-15 0.22720865958314818 1106 16-17 0.07513136981369684 1106 18-19 0.07205760252045224 1106 20-21 0.11746515077644659 1106 22-23 0.14643605350804023 1106 24-25 0.13903060888723928 1106 26-27 0.1314763230789353 1106 28-29 0.1583787822898941 1106 30-31 0.1944351477715216 1106 32-33 0.1332408984658997 1106 34-35 0.23458425695658036 1106 36-37 0.26655035807220884 1106 38-39 0.023900403805498627 1106 40-41 0.4756434248491175 1106 42-43 0.289390946620955 1106 44-45 0.4753095087669337 1106 46-47 0.5113798798618276 1106 48-49 0.5798479929445577 1106 50-51 0.5002177401046097 1106 52-53 0.6135069635678434 1106 54-55 0.20916372318350795 1106 56-57 0.5650033238836514 1106 58-59 0.8800617656367535 1106 60-61 0.9590961688395296 1106 62-63 0.8603620967739118 1106 64-65 1.104143778846229 1106 66-67 1.1624524379033225 1106 68-69 0.948994321524566 1106 70-71 1.1484643942282737 1106 72-73 1.3271038189395803 1106 74-75 1.5462081801817185 1106 76-77 1.7815832238988598 1106 78-79 1.991782635789317 1106 80-81 1.988699643364388 1106 82-83 2.0213000776340913 1106 84-85 2.3074612287086538 1106 86-87 2.1188313764000206 1106 88-89 2.249112367781823 1106 90-91 2.584556950575678 1106 92-93 2.9073878126153296 1106 94-95 2.9860165337358637 1106 96-97 3.0784941724727872 1106 98-99 3.3110879486251967 1106 100-101 3.2199863220555542 1107 1 0.29449260741633765 1107 2 0.1860385098357824 1107 3 0.03798598056873459 1107 4 0.1272454984982332 1107 5 0.1081728460509197 1107 6 0.09799497690760006 1107 7 0.12259447599179651 1107 8 0.1476102715010441 1107 9 0.17895529971887925 1107 10-11 0.17327839975088466 1107 12-13 0.16206582845548212 1107 14-15 0.3159153460078059 1107 16-17 0.3872000232251125 1107 18-19 0.2241606078963585 1107 20-21 0.32502626654173383 1107 22-23 0.31116835235329177 1107 24-25 0.3532990930647202 1107 26-27 0.31907214864168054 1107 28-29 0.4676468665364055 1107 30-31 0.4524564998749412 1107 32-33 0.5445376851258814 1107 34-35 0.5538551407198042 1107 36-37 0.57300219165041 1107 38-39 0.605439064499862 1107 40-41 0.7260112560866716 1107 42-43 0.8238730991756711 1107 44-45 0.7685013156550085 1107 46-47 0.9110662497937554 1107 48-49 1.1008094269706845 1107 50-51 0.8653115554880557 1107 52-53 0.8480859415127 1107 54-55 1.0415631826452483 1107 56-57 1.179587964008931 1107 58-59 1.024749103822586 1107 60-61 1.17082961696007 1107 62-63 1.2158529383549492 1107 64-65 1.3383227205868096 1107 66-67 1.4815999745391046 1107 68-69 1.4901301675469334 1107 70-71 1.7668811096584456 1107 72-73 1.872041955934879 1107 74-75 2.0027338957423986 1107 76-77 2.0966333626117546 1107 78-79 2.1277150642963996 1107 80-81 2.2998309849012166 1107 82-83 2.3104660531919947 1107 84-85 2.6209136432391986 1107 86-87 2.8577110855870664 1107 88-89 3.117339326496321 1107 90-91 3.2186169654783647 1107 92-93 3.4395879380673584 1107 94-95 3.769810910285596 1107 96-97 3.880672428016041 1107 98-99 4.050043749939494 1107 100-101 3.7219634544242837 1108 1 0.2573162925760357 1108 2 0.19709856299157735 1108 3 0.14815096907313574 1108 4 0.16914286629887698 1108 5 0.12137494890879452 1108 6 0.23002406550126864 1108 7 0.17920922944601614 1108 8 0.11753724776631458 1108 9 0.23132907484849596 1108 10-11 0.1902096699890805 1108 12-13 0.21327870956824313 1108 14-15 0.2837884793587335 1108 16-17 0.41180281561725707 1108 18-19 0.32300379426204273 1108 20-21 0.3171386856141467 1108 22-23 0.22058542185278185 1108 24-25 0.2758305623852806 1108 26-27 0.2570968807973131 1108 28-29 0.29834182116859154 1108 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2851.0 6 3207.0 7 4055.0 8 5064.0 9 6422.0 10 8162.0 11 10571.0 12 13545.0 13 16980.0 14 19826.0 15 24653.0 16 29580.0 17 36409.0 18 35254.0 19 30194.0 20 30118.0 21 31212.0 22 33843.0 23 38205.0 24 42686.0 25 47826.0 26 54138.0 27 62130.0 28 72500.0 29 84432.0 30 99499.0 31 119156.0 32 146918.0 33 184695.0 34 241057.0 35 323958.0 36 414221.0 37 441848.0 38 221008.0 39 12385.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.611934860174514 18.44256636066288 14.86795719995508 23.07754157920753 2 15.470893829722975 19.575898603220033 40.35061048736966 24.602597079687335 3 19.2454885441067 23.530556538185916 30.00409157146414 27.21986334624324 4 12.845520269249782 16.67518687295815 36.89436284455731 33.58493001323476 5 13.603015231624244 37.075391605640576 34.40739018346354 14.914202979271634 6 32.277141122888885 36.230916688409216 16.997074556891352 14.494867631810543 7 28.378445491244864 33.19492864500684 20.20339070089418 18.223235162854117 8 26.722286014659534 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29.419094083200175 22.530736408553643 32-33 21.469542889962497 26.21595324166357 29.46633332207169 22.848170546302242 34-35 22.442151340364457 26.400774108450864 29.127469092422707 22.029605458761967 36-37 22.608582253621577 25.717773727011096 29.28028619561892 22.393357823748406 38-39 21.903135569904865 26.259986282715563 29.08863152607576 22.74824662130381 40-41 22.506107359484286 26.091264597074716 29.205549273914542 22.197078769526453 42-43 22.15578793201781 26.1875797882806 28.921580797208108 22.73505148249349 44-45 22.2189179841964 26.386513440086677 29.145270740369977 22.249297835346947 46-47 22.797017111379684 26.74680778764027 28.278701537517826 22.177473563462215 48-49 22.386984097120035 26.809038941544195 28.119999263638274 22.683977697697504 50-51 22.527637224394013 27.10017658980217 28.14488841169319 22.22729777411062 52-53 23.00656694076345 26.625181474495797 28.154118350163998 22.214133234576757 54-55 22.258783206535114 26.970810149194147 28.337735005647957 22.432671638622786 56-57 22.42402115210191 27.04272655011305 28.128566871467747 22.404685426317293 58-59 22.515092717837522 26.533107723587534 27.977229627152877 22.974569931422067 60-61 21.935678566434724 26.88547905860133 28.50800429345451 22.670838081509437 62-63 22.129279685489344 26.92452014496131 28.61911854606372 22.327081623485622 64-65 21.843583579678683 26.964746650589838 28.340953785021362 22.850715984710124 66-67 21.721054169108122 26.966350951788325 28.29076614190115 23.0218287372024 68-69 22.531209123672326 26.46692084502314 28.478563875399242 22.52330615590529 70-71 22.188870758932868 26.779472446426094 27.960805519462646 23.070851275178395 72-73 22.22890211206859 26.83359598810319 28.133905972519536 22.803595927308688 74-75 22.261001958200293 27.29011152757861 27.830524823346558 22.618361690874544 76-77 22.37743249664556 26.772044318796244 27.9909902090543 22.859532975503893 78-79 22.43179629791619 27.075494288339957 27.81873791952576 22.67397149421809 80-81 22.76743970436024 27.13294923221714 27.409533108920648 22.69007795450197 82-83 22.583037669862254 27.19584376810077 27.600709930070877 22.620408631966104 84-85 22.508145715214482 27.42992236346059 27.556386755772078 22.50554516555285 86-87 22.900975004276187 27.282491545139393 27.10029983116451 22.716233619419913 88-89 22.59058436699965 27.34741423252197 26.95160850650432 23.11039289397406 90-91 22.514792684535497 27.356665967983652 27.11532475332577 23.013216594155082 92-93 22.822014506337023 26.827182072261486 27.37114437942768 22.979659041973807 94-95 22.454769110407657 26.58825373415158 27.65505043220121 23.301926723239553 96-97 22.88334141764072 26.09952210258648 27.61798134619256 23.399155133580233 98-99 22.61182430886209 26.54284030729888 27.050090514649117 23.795244869189908 100-101 23.332483321620305 25.354214761659648 27.445108295325184 23.868193621394862 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 753.0 1 529.0 2 445.5 3 597.5 4 505.5 5 487.0 6 894.5 7 2108.5 8 3668.5 9 4265.5 10 3486.5 11 2683.0 12 2598.0 13 2703.0 14 2944.0 15 3238.0 16 3507.0 17 3834.0 18 4299.5 19 4890.5 20 5432.5 21 6513.0 22 8124.0 23 10026.5 24 12636.0 25 16067.5 26 20477.0 27 25542.0 28 29763.5 29 33190.5 30 37088.5 31 43847.5 32 53744.0 33 57502.0 34 56281.5 35 58433.0 36 62913.5 37 67720.5 38 72636.5 39 75937.5 40 79011.0 41 84713.0 42 88822.5 43 91138.0 44 94193.5 45 100666.5 46 104758.0 47 107008.0 48 109443.0 49 106169.5 50 102312.0 51 99993.0 52 98770.5 53 100698.0 54 103487.5 55 99602.0 56 91322.0 57 83802.5 58 78700.5 59 69986.5 60 55882.0 61 46865.5 62 41208.0 63 31385.0 64 21778.0 65 16749.0 66 12869.5 67 9817.0 68 7543.5 69 5646.5 70 4299.0 71 3062.5 72 2075.5 73 1574.5 74 1196.5 75 894.0 76 663.5 77 445.5 78 291.0 79 176.5 80 95.0 81 59.0 82 43.0 83 28.5 84 20.0 85 17.0 86 12.5 87 14.0 88 10.5 89 6.5 90 5.0 91 4.0 92 4.0 93 3.0 94 2.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46317761591850554 2 0.04015151540734202 3 0.052004485893445515 4 0.05578663032205972 5 0.08236294876241143 6 0.0815524892419941 7 0.04606111607705174 8 0.03923974844687252 9 0.04484542679642574 10-11 0.04906657013193268 12-13 0.045031157103188044 14-15 0.038311096913061 16-17 0.03866567295324358 18-19 0.04695599846417921 20-21 0.05576974574871771 22-23 0.04781711170462262 24-25 0.06703175616785022 26-27 0.057204934482790064 28-29 0.04518311826326629 30-31 0.0471923824909676 32-33 0.046702729864048796 34-35 0.05034979770592679 36-37 0.04557146345013293 38-39 0.026491895573641563 40-41 0.02416182445244173 42-43 0.02409428615907362 44-45 0.014824655394300376 46-47 0.026914009907192255 48-49 0.026390588133589396 50-51 0.0157533069281119 52-53 0.015787076074795958 54-55 0.01609099839495246 56-57 0.01494284740769457 58-59 0.01411550331393521 60-61 0.016783265901975597 62-63 0.016648189315239374 64-65 0.016901457915369792 66-67 0.009387822778167437 68-69 0.012494584273100545 70-71 0.018910722143071095 72-73 0.016513112728503154 74-75 0.013541427820306266 76-77 0.009522899364903657 78-79 0.013845350140462767 80-81 0.017475533408998736 82-83 0.014453194780775764 84-85 0.012511468846442572 86-87 0.003934105588692469 88-89 0.029244081028392085 90-91 0.03213134306987883 92-93 0.017154726515500207 94-95 0.032823610576901974 96-97 0.027521854547505255 98-99 0.02406051701238956 100-101 0.1506441633575717 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2961283.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.21207975094161 #Duplication Level Percentage of deduplicated Percentage of total 1 72.23532453731775 35.5485055196563 2 14.737183748129295 14.504949238344391 3 5.449437841933643 8.045345090251129 4 2.567223089641188 5.053535497035235 5 1.4151935752607432 3.4822309544375942 6 0.8557462958332142 2.5267832974270203 7 0.5617997977603315 1.9353135516011015 8 0.3836557312170737 1.5104397153248361 9 0.28349239858883946 1.2556125475325726 >10 1.3681658145488 12.003016093149261 >50 0.08943629298978653 3.041312981277384 >100 0.04478624143430092 4.257311413538368 >500 0.004277317672608502 1.4271268843762714 >1k 0.004001361693453882 4.167407417488186 >5k 1.3797798942944423E-4 0.4144388694339483 >10k+ 1.3797798942944423E-4 0.8266709291265018 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13409 0.4528104878865006 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10940 0.36943446472356745 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6870 0.23199403771946145 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5337 0.18022593585280436 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4864 0.1642531294712461 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4840 0.16344266995082873 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4834 0.16324005507072442 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4467 0.15084677823767603 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3757 0.12687068409199662 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3756 0.12683691494531255 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3602 0.121636466355968 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3293 0.11120180003059484 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3175 0.1072170407218763 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 3.376914668405553E-5 6 0.0 0.0 0.0 0.0 3.376914668405553E-5 7 0.0 0.0 0.0 0.0 3.376914668405553E-5 8 0.0 0.0 0.0 0.0 3.376914668405553E-5 9 0.0 0.0 0.0 0.0 3.376914668405553E-5 10-11 0.0 0.0 0.0 0.0 6.753829336811106E-5 12-13 0.0 0.0 0.0 0.0 5.065372002608329E-4 14-15 0.0 0.0 0.0 0.0 6.078446403129994E-4 16-17 0.0 0.0 0.0 0.0 7.429212270492216E-4 18-19 0.0 0.0 0.0 0.0 8.61113240443416E-4 20-21 0.0 0.0 0.0 0.0 9.793052538376102E-4 22-23 0.0 0.0 0.0 5.065372002608329E-5 9.793052538376102E-4 24-25 0.0 0.0 0.0 3.039223201564997E-4 9.793052538376102E-4 26-27 0.0 0.0 0.0 0.0010130744005216658 0.0010637281205477492 28-29 0.0 0.0 0.0 0.0029716849081968862 0.001080612693889777 30-31 0.0 0.0 0.0 0.01035024345866302 0.001080612693889777 32-33 0.0 0.0 0.0 0.027437431680795116 0.001148150987257888 34-35 0.0 0.0 0.0 0.05212267790683971 0.001148150987257888 36-37 0.0 0.0 0.0 0.09239238532757592 0.001148150987257888 38-39 0.0 0.0 0.0 0.15280538874535127 0.0012156892806259988 40-41 0.0 0.0 0.0 0.21630826908471767 0.0012156892806259988 42-43 0.0 0.0 0.0 0.2829685646390433 0.0012156892806259988 44-45 0.0 0.0 0.0 0.3638963246673823 0.0012325738539680268 46-47 0.0 0.0 0.0 0.4582810896493175 0.0012832275739941101 48-49 0.0 0.0 0.0 0.5557219624061598 0.001350765867362221 50-51 0.0 0.0 0.0 0.6604738554201 0.001418304160730332 52-53 0.0 0.0 0.0 0.7592654940443044 0.0015871498941506096 54-55 0.0 0.0 0.0 0.8656045369523953 0.0016040344674926373 56-57 1.6884573342027764E-5 0.0 0.0 0.9873929644684416 0.001705341907544804 58-59 3.376914668405553E-5 0.0 0.0 1.1162897973614814 0.0017897647742549428 60-61 3.376914668405553E-5 3.376914668405553E-5 0.0 1.2575461379408857 0.0017897647742549428 62-63 3.376914668405553E-5 3.376914668405553E-5 0.0 1.3933318767574732 0.0018235339209389985 64-65 3.376914668405553E-5 3.376914668405553E-5 0.0 1.5339297189765382 0.0018235339209389985 66-67 3.376914668405553E-5 3.376914668405553E-5 0.0 1.6940967816990136 0.0018573030676230541 68-69 3.376914668405553E-5 3.376914668405553E-5 0.0 1.8753020228056556 0.0018910722143071095 70-71 3.376914668405553E-5 3.376914668405553E-5 0.0 2.0601374471808334 0.0019417259343331927 72-73 3.376914668405553E-5 3.376914668405553E-5 0.0 2.2518280083328746 0.001992379654359276 74-75 3.376914668405553E-5 3.376914668405553E-5 0.0 2.439398733589461 0.002043033374385359 76-77 3.376914668405553E-5 3.376914668405553E-5 0.0 2.6441748390815736 0.002144340814437526 78-79 3.376914668405553E-5 3.376914668405553E-5 0.0 2.87645591454785 0.002161225387779554 80-81 3.376914668405553E-5 3.376914668405553E-5 0.0 3.115085589590728 0.0021781099611215817 82-83 3.376914668405553E-5 3.376914668405553E-5 0.0 3.3635252017453245 0.0021949945344636092 84-85 3.376914668405553E-5 3.376914668405553E-5 0.0 3.6181445677431032 0.0021949945344636092 86-87 3.376914668405553E-5 3.376914668405553E-5 0.0 3.8787579572773017 0.0022456482544896926 88-89 3.376914668405553E-5 5.065372002608329E-5 0.0 4.156931303087209 0.002296301974515776 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7535 0.0 47.34636 1 GTATCAA 12930 0.0 39.078365 1 TCAACGC 15800 0.0 31.525593 4 TATCAAC 15880 0.0 31.485964 2 ATCAACG 15825 0.0 31.475788 3 CAACGCA 15995 0.0 31.11154 5 TAAGGTG 2080 0.0 30.619675 5 AACGCAG 16560 0.0 30.016796 6 GTAAGGT 2305 0.0 27.836967 4 ACGCAGA 18310 0.0 26.801443 7 CGCAGAG 18370 0.0 26.558294 8 GGTAAGG 2310 0.0 25.924932 3 AGGTAAG 2315 0.0 24.841976 2 GCAGAGT 19970 0.0 23.787756 9 AAGGTAA 2360 0.0 23.47444 1 GAGTACT 13330 0.0 23.378477 12-13 GTGTAAG 1330 0.0 22.263288 1 GTACTTT 13955 0.0 22.196764 14-15 GTACATG 8965 0.0 22.16115 1 AAGGTGT 3180 0.0 21.519312 6 >>END_MODULE