FastQCFastQC Report
Thu 26 May 2016
SRR938238_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938238_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2961283
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127940.43204246267580637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109690.37041376997740505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69820.2357761821480757No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA53510.18069870390638113No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC52630.17772701899818424No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA49130.1659078176587648No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA47190.15935660320205802No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA46170.15591215024028438No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG38280.12926829350656455No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA36430.12302100137001429No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA36150.12207546526286073No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA34560.1167061709400959No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT31040.10481943130730834No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA30200.10198282298584768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76700.046.885631
GTATCAA125350.036.3440671
TCAACGC156450.028.9313414
ATCAACG155700.028.8891413
TATCAAC156150.028.8102632
CAACGCA159450.028.387015
AACGCAG164200.027.5658236
TAAGGTG25000.027.1673495
AGGTAAG22800.026.4611132
ACGCAGA183750.024.3486447
GTAAGGT26850.024.2341354
CGCAGAG184700.024.1205488
GTGTAAG13150.023.842811
AAGGTAA23800.023.7524971
GGTAAGG26600.023.7488863
GAGTACT129700.022.33783512-13
GTACATG90500.021.9940221
GCAGAGT200600.021.9719359
GTACTTT133150.021.65203714-15
TACATGG90500.021.311632
Produced by FastQC (version 0.11.5)