##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938238_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2961283 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52142399088503 34.0 31.0 34.0 31.0 34.0 2 32.750705690742834 34.0 31.0 34.0 31.0 34.0 3 32.70981192949137 34.0 31.0 34.0 31.0 34.0 4 36.23347988017356 37.0 35.0 37.0 35.0 37.0 5 36.19979279251595 37.0 35.0 37.0 35.0 37.0 6 36.25882902782341 37.0 37.0 37.0 35.0 37.0 7 36.22305770843246 37.0 37.0 37.0 35.0 37.0 8 36.213476388443794 37.0 37.0 37.0 35.0 37.0 9 37.92193113592993 39.0 38.0 39.0 35.0 39.0 10-11 37.88868254064201 39.0 38.0 39.0 35.0 39.0 12-13 37.83285032197193 39.0 38.0 39.0 35.0 39.0 14-15 39.12092647004694 40.0 38.5 41.0 36.0 41.0 16-17 39.06391401970025 40.0 38.0 41.0 36.0 41.0 18-19 39.06333960651515 40.0 38.5 41.0 36.0 41.0 20-21 39.062438983373085 40.0 38.5 41.0 35.5 41.0 22-23 39.091857819735566 40.0 39.0 41.0 35.5 41.0 24-25 39.04380499938709 40.0 39.0 41.0 35.0 41.0 26-27 38.87609255852953 40.0 38.0 41.0 35.0 41.0 28-29 38.797548900257084 40.0 38.0 41.0 35.0 41.0 30-31 38.567055056879056 40.0 38.0 41.0 34.5 41.0 32-33 38.353458112581606 40.0 38.0 41.0 34.0 41.0 34-35 38.14397273073867 40.0 38.0 41.0 34.0 41.0 36-37 38.04822892644843 40.0 38.0 41.0 34.0 41.0 38-39 37.981370068311605 40.0 38.0 41.0 33.0 41.0 40-41 37.8479370259445 40.0 37.5 41.0 33.0 41.0 42-43 37.649386600335056 40.0 37.0 41.0 33.0 41.0 44-45 37.39898516960385 40.0 37.0 41.0 32.5 41.0 46-47 37.042251787485355 40.0 36.5 41.0 31.5 41.0 48-49 36.65245689115157 40.0 36.0 41.0 31.0 41.0 50-51 36.33629950261424 39.0 35.5 41.0 30.0 41.0 52-53 35.861911205379556 39.0 35.0 40.0 29.0 41.0 54-55 35.53858040585787 39.0 35.0 40.0 28.0 41.0 56-57 35.25509753711482 38.0 35.0 40.0 28.0 41.0 58-59 34.87109590674042 38.0 34.0 40.0 26.5 41.0 60-61 34.44886794001114 37.5 34.0 40.0 26.0 41.0 62-63 34.01789258237055 37.0 33.5 40.0 25.5 41.0 64-65 33.58623069797787 36.0 33.0 39.0 24.5 41.0 66-67 33.079217352748785 36.0 32.5 39.0 22.5 40.0 68-69 32.55607822690368 35.0 32.0 38.5 21.5 40.0 70-71 31.95777826705519 35.0 31.0 37.5 19.5 40.0 72-73 31.4965720939201 35.0 31.0 37.0 18.0 39.0 74-75 30.95793681319887 34.5 30.5 36.5 15.5 39.0 76-77 29.76396480849686 33.5 29.0 35.0 14.0 37.5 78-79 30.458071720939877 34.0 30.0 36.0 14.0 37.5 80-81 30.27972638886591 34.0 30.0 35.0 9.5 37.0 82-83 29.834261196920387 34.0 30.0 35.0 7.0 37.0 84-85 29.342048024454265 34.0 29.0 35.0 2.0 36.0 86-87 28.828022684761976 34.0 29.0 35.0 2.0 36.0 88-89 28.21505594027994 33.5 27.5 35.0 2.0 35.0 90-91 27.699924829879482 33.0 27.0 35.0 2.0 35.0 92-93 27.088115691745774 33.0 25.5 35.0 2.0 35.0 94-95 26.362832596546838 32.0 25.0 34.5 2.0 35.0 96-97 25.562567137284752 32.0 22.5 34.0 2.0 35.0 98-99 24.54962967065289 32.0 10.0 34.0 2.0 35.0 100-101 21.038092103996817 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.001277611122041833 1101 2 0.011030420359489312 1101 3 0.10490723550676506 1101 4 0.012667019729661888 1101 5 0.017583827765783155 1101 6 0.05290793936631388 1101 7 -6.039766409031699E-4 1101 8 0.060601013723768915 1101 9 0.1301490514110597 1101 10-11 0.12946214386749944 1101 12-13 0.0930126556104156 1101 14-15 0.2048190342054923 1101 16-17 0.1939458452471996 1101 18-19 0.20026887511134106 1101 20-21 0.1627625541862372 1101 22-23 0.07897411947533328 1101 24-25 0.1643222317097468 1101 26-27 0.15060263840345556 1101 28-29 0.17538347685405142 1101 30-31 0.2258444401633568 1101 32-33 0.26498877566476864 1101 34-35 0.24320466067020163 1101 36-37 0.22699161660807476 1101 38-39 0.36048199078175713 1101 40-41 0.3691730956714494 1101 42-43 0.41579333025621423 1101 44-45 0.4304477615003677 1101 46-47 0.5553430571684288 1101 48-49 0.4820971369192435 1101 50-51 0.5426819779709646 1101 52-53 0.530762770171215 1101 54-55 0.5430622844019197 1101 56-57 0.628800947393124 1101 58-59 0.5252137352679611 1101 60-61 0.7398706869293008 1101 62-63 0.7826222473403064 1101 64-65 0.8159318379505791 1101 66-67 0.7309549452935187 1101 68-69 0.7970914868874317 1101 70-71 0.931764958241633 1101 72-73 0.8871920839112981 1101 74-75 0.9641888060117694 1101 76-77 1.0321750935329952 1101 78-79 0.9969746893927969 1101 80-81 1.1400119364145063 1101 82-83 1.2410150726026856 1101 84-85 1.2769172431073486 1101 86-87 1.3470712989519917 1101 88-89 1.5715052329652153 1101 90-91 1.8048759387669477 1101 92-93 2.0551562537850643 1101 94-95 1.9132160606472581 1101 96-97 1.9841450906125395 1101 98-99 2.2787001485560197 1101 100-101 2.615376918696523 1102 1 0.08983730716677485 1102 2 0.1101722236552618 1102 3 0.21142799844812998 1102 4 0.076910240243663 1102 5 -0.012882480042243571 1102 6 0.043124588967032196 1102 7 0.13412270154019978 1102 8 0.11146282391629825 1102 9 0.05661050442312643 1102 10-11 0.13654830269154417 1102 12-13 0.12415781200679277 1102 14-15 0.2223137987075603 1102 16-17 0.18086799501018191 1102 18-19 0.24246311783652175 1102 20-21 0.19593803086218742 1102 22-23 0.2666074091490671 1102 24-25 0.1983124349610179 1102 26-27 0.2065718504004579 1102 28-29 0.11584915940680673 1102 30-31 0.08235762425285031 1102 32-33 0.20934768735246934 1102 34-35 0.09043199185060047 1102 36-37 0.25117837196896176 1102 38-39 0.2715792163535653 1102 40-41 0.22452367035142373 1102 42-43 0.2794466724892857 1102 44-45 0.31089800484917873 1102 46-47 0.36476707525373087 1102 48-49 0.4518839762971112 1102 50-51 0.4651533684818645 1102 52-53 0.37208705543812215 1102 54-55 0.41994755763711566 1102 56-57 0.470351041844566 1102 58-59 0.4431682654048572 1102 60-61 0.5610557297403389 1102 62-63 0.575859529553199 1102 64-65 0.46528718893126353 1102 66-67 0.48964890340575096 1102 68-69 0.5854207181671924 1102 70-71 0.7478631027492249 1102 72-73 0.666811252018622 1102 74-75 0.6892972174275762 1102 76-77 0.8036547100755698 1102 78-79 0.9626622496006867 1102 80-81 0.9836339671624508 1102 82-83 1.0275971755229492 1102 84-85 1.0698400706362285 1102 86-87 1.1843082146732655 1102 88-89 1.3160420311330832 1102 90-91 1.3370264433966774 1102 92-93 1.3954826343338347 1102 94-95 1.572714486817464 1102 96-97 1.8530301413543064 1102 98-99 1.9949698593226692 1102 100-101 2.3281658387399027 1103 1 0.27242716682481216 1103 2 0.3058015812745012 1103 3 0.39336541809600334 1103 4 0.22232385000113908 1103 5 0.1283864997391717 1103 6 0.17940885008673746 1103 7 0.22514866826768554 1103 8 0.21015704574846694 1103 9 0.2479515284652507 1103 10-11 0.33008609930475075 1103 12-13 0.3219343837007784 1103 14-15 0.46056939737419356 1103 16-17 0.3894733387312357 1103 18-19 0.4587853128576995 1103 20-21 0.4658186863045657 1103 22-23 0.4609265940930385 1103 24-25 0.4793748664772721 1103 26-27 0.4745131487624903 1103 28-29 0.5138545662547997 1103 30-31 0.5199217776461822 1103 32-33 0.5719688089590775 1103 34-35 0.5129183335931629 1103 36-37 0.6131788094951034 1103 38-39 0.5716265615578422 1103 40-41 0.5598192956032264 1103 42-43 0.6441717046301321 1103 44-45 0.698579526875605 1103 46-47 0.7723501025753734 1103 48-49 0.8070252933919448 1103 50-51 0.9076428986480707 1103 52-53 0.8695656666967793 1103 54-55 0.9774939533711802 1103 56-57 0.9469899044305663 1103 58-59 1.1061834813066937 1103 60-61 1.0912009281154624 1103 62-63 1.1860611059303778 1103 64-65 1.0483416153870948 1103 66-67 1.0603129038573869 1103 68-69 1.2022102065053915 1103 70-71 1.1853584516282147 1103 72-73 1.035543946640768 1103 74-75 1.0299116401368522 1103 76-77 0.8563434108667103 1103 78-79 1.141530731295159 1103 80-81 1.187522913789394 1103 82-83 1.2502904440356986 1103 84-85 1.4127495809918678 1103 86-87 1.4693499207942686 1103 88-89 1.5837833312914142 1103 90-91 1.6510767101464872 1103 92-93 1.8934677366328323 1103 94-95 1.973729168963061 1103 96-97 2.193878884332719 1103 98-99 2.4554019709187784 1103 100-101 2.7760848309670756 1104 1 0.11730561772332493 1104 2 0.12292639939963124 1104 3 0.16867200716272635 1104 4 0.07594882874303721 1104 5 0.05995785137651666 1104 6 0.05730231392907115 1104 7 0.057944250361749994 1104 8 0.004878186694405429 1104 9 0.11621389123239112 1104 10-11 0.13376103458564614 1104 12-13 0.05093374123370609 1104 14-15 0.15156340258951673 1104 16-17 0.16873275370827656 1104 18-19 0.12495635081995005 1104 20-21 0.1395097777427523 1104 22-23 0.18686183246721555 1104 24-25 0.20671550157486962 1104 26-27 0.1348517903225499 1104 28-29 0.08576481927345014 1104 30-31 0.20614038651601163 1104 32-33 0.2610802911684047 1104 34-35 0.26112379550809806 1104 36-37 0.24295479634342598 1104 38-39 0.23054386281820882 1104 40-41 0.25251394895445145 1104 42-43 0.29828703691788405 1104 44-45 0.25648541489737653 1104 46-47 0.2626800283137456 1104 48-49 0.19824876800504398 1104 50-51 0.29398704841848655 1104 52-53 0.3817465589701712 1104 54-55 0.41803917643952104 1104 56-57 0.632928614961358 1104 58-59 0.6775587387659243 1104 60-61 0.6989068718806948 1104 62-63 0.6075159631704139 1104 64-65 0.592177002095589 1104 66-67 0.5867390862802466 1104 68-69 0.8149566823845333 1104 70-71 0.7932763859075997 1104 72-73 0.8177157215505382 1104 74-75 0.7431115040850038 1104 76-77 0.7844904366220824 1104 78-79 0.8374064233644667 1104 80-81 0.9025102297887138 1104 82-83 1.0432005128174637 1104 84-85 1.2352296692366131 1104 86-87 1.2539445287948716 1104 88-89 1.3762375318923077 1104 90-91 1.28694845765693 1104 92-93 1.4490163373773406 1104 94-95 1.4976356758317912 1104 96-97 1.8056362679800415 1104 98-99 2.1459094018063283 1104 100-101 2.31687853762417 1105 1 0.1385796720708754 1105 2 0.12600854408115225 1105 3 0.1350115489914785 1105 4 0.08241653242133395 1105 5 0.03506381196078934 1105 6 0.0661015523345796 1105 7 0.04772550627821914 1105 8 0.07687221932294364 1105 9 0.058398393927625136 1105 10-11 0.04088711830388547 1105 12-13 0.005410877324067087 1105 14-15 0.1107890062031558 1105 16-17 0.15186590950293777 1105 18-19 0.19879699110229865 1105 20-21 0.17543624297267257 1105 22-23 0.14892287523009884 1105 24-25 0.08729919570109246 1105 26-27 0.12447665055996282 1105 28-29 0.14979104462968706 1105 30-31 0.15918484441576197 1105 32-33 0.12923248236005946 1105 34-35 0.1185615989224047 1105 36-37 0.18196405371735125 1105 38-39 0.27648445166078517 1105 40-41 0.31206185492682437 1105 42-43 0.25022701312698103 1105 44-45 0.31649380065284305 1105 46-47 0.30772488630066164 1105 48-49 0.46870701962414785 1105 50-51 0.479422647781206 1105 52-53 0.5013080973767572 1105 54-55 0.5185812811480517 1105 56-57 0.5017257011122709 1105 58-59 0.6497917520295218 1105 60-61 0.592762207328775 1105 62-63 0.46464108685807304 1105 64-65 0.6768883142028699 1105 66-67 0.8069017824390485 1105 68-69 0.7425657999065649 1105 70-71 0.8933155908772612 1105 72-73 0.8483991918780056 1105 74-75 0.7145816279334127 1105 76-77 0.8494136207800587 1105 78-79 1.0202400049661726 1105 80-81 1.119657648512078 1105 82-83 1.2076118890458574 1105 84-85 1.4482817761241868 1105 86-87 1.5398013359921556 1105 88-89 1.5860992115796684 1105 90-91 1.7727704804904754 1105 92-93 1.8330924218100186 1105 94-95 1.8297248765147955 1105 96-97 2.2148874798511997 1105 98-99 2.435691171227525 1105 100-101 2.7742271698261867 1106 1 0.06833232564857639 1106 2 0.09942177282990627 1106 3 0.15041358019643525 1106 4 0.10509396483628564 1106 5 0.0629132981108782 1106 6 -0.02436865885228201 1106 7 0.020894139203903705 1106 8 0.0030117864059917565 1106 9 -0.004305997008906104 1106 10-11 0.025830898792818857 1106 12-13 0.029445728255275583 1106 14-15 0.02983993025880949 1106 16-17 0.03968826052796004 1106 18-19 0.008909063672199125 1106 20-21 -0.0344541260005613 1106 22-23 0.049215750030157324 1106 24-25 0.041133500132822576 1106 26-27 0.019328372068635247 1106 28-29 0.06719469970366276 1106 30-31 0.055299943086339454 1106 32-33 0.09608036627505356 1106 34-35 -0.024114367683637283 1106 36-37 0.10603912609166599 1106 38-39 0.06755116318473142 1106 40-41 0.2068660038724346 1106 42-43 0.2770844292149448 1106 44-45 0.3588670468147015 1106 46-47 0.15815034616508683 1106 48-49 0.32821707299812886 1106 50-51 0.3499358707461937 1106 52-53 0.3247218338605151 1106 54-55 0.2967212800266168 1106 56-57 0.501743582214857 1106 58-59 0.4236644588884957 1106 60-61 0.5721550092357361 1106 62-63 0.5119953238625996 1106 64-65 0.37427758492319896 1106 66-67 0.4085635770571443 1106 68-69 0.18721341194050467 1106 70-71 0.43646721688760337 1106 72-73 0.5195423153136822 1106 74-75 0.5733779824843275 1106 76-77 0.5144839023434038 1106 78-79 0.8267863428501379 1106 80-81 1.0288715378461575 1106 82-83 0.9639573625440789 1106 84-85 0.9762825893832208 1106 86-87 1.1321797478058677 1106 88-89 1.2875196775391267 1106 90-91 1.0273216626079567 1106 92-93 1.1450522083140697 1106 94-95 1.4648121914840146 1106 96-97 1.4515609810726318 1106 98-99 1.6660060640667425 1106 100-101 2.0789838521708255 1107 1 0.0871764409802438 1107 2 0.12001800674320862 1107 3 0.12960306748494332 1107 4 0.0386744835677888 1107 5 0.05281651481872984 1107 6 0.061927812072738675 1107 7 -0.007668508928418305 1107 8 0.007652037983618243 1107 9 0.029153279494934736 1107 10-11 -0.01584832710923223 1107 12-13 0.05511729653191111 1107 14-15 0.05862959183301797 1107 16-17 0.17113704200147595 1107 18-19 0.12060504457654986 1107 20-21 0.03177854541194591 1107 22-23 0.0473383899112676 1107 24-25 0.048467391966234175 1107 26-27 0.1261975020293491 1107 28-29 0.1519732221482002 1107 30-31 0.1635346267830471 1107 32-33 0.15385954965813653 1107 34-35 0.2109613666106469 1107 36-37 0.2673608558141112 1107 38-39 0.24277733466670526 1107 40-41 0.2040094443732272 1107 42-43 0.2006798295879335 1107 44-45 0.2677013112153688 1107 46-47 0.3348005875971012 1107 48-49 0.30609857555979403 1107 50-51 0.26146146001288173 1107 52-53 0.2018345217754458 1107 54-55 0.2867355301981718 1107 56-57 0.29859970389490087 1107 58-59 0.4745515689031876 1107 60-61 0.6355351204586412 1107 62-63 0.537201919776571 1107 64-65 0.4196609082231859 1107 66-67 0.540518165949166 1107 68-69 0.5375253580887502 1107 70-71 0.6131127222950816 1107 72-73 0.514087362274001 1107 74-75 0.603791363753249 1107 76-77 0.6819811975471808 1107 78-79 0.885934132073892 1107 80-81 0.9274508357357867 1107 82-83 1.0211708541097408 1107 84-85 1.2511445079572745 1107 86-87 1.373941591024515 1107 88-89 1.1933121512594553 1107 90-91 1.2757331999846642 1107 92-93 1.3848000834177334 1107 94-95 1.4095321556165246 1107 96-97 1.5234902480202308 1107 98-99 1.5145048495395628 1107 100-101 2.057749096907525 1108 1 0.04412840530661555 1108 2 0.07334115087441262 1108 3 0.17013980402522577 1108 4 0.08236570842809954 1108 5 0.049196157639173066 1108 6 0.02523832213063315 1108 7 0.03792820419752019 1108 8 0.05960336758381857 1108 9 -0.015521670180348224 1108 10-11 0.0812616034335889 1108 12-13 0.06794863870573664 1108 14-15 0.19731257532811952 1108 16-17 0.16218624436225326 1108 18-19 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-2.6868684128866605 2316 98-99 -3.242593970484471 2316 100-101 -3.8847813233506443 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 8.0 4 23.0 5 38.0 6 103.0 7 260.0 8 687.0 9 1301.0 10 2231.0 11 3623.0 12 5714.0 13 8392.0 14 11623.0 15 15899.0 16 20679.0 17 28980.0 18 28591.0 19 21934.0 20 19287.0 21 18567.0 22 19710.0 23 21357.0 24 24421.0 25 27993.0 26 32344.0 27 38629.0 28 47186.0 29 57934.0 30 70457.0 31 87339.0 32 110534.0 33 145543.0 34 203530.0 35 304366.0 36 471822.0 37 652675.0 38 427109.0 39 30391.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.87790123402593 18.856252509469712 15.248289339451853 23.017556917052506 2 15.63193616064131 19.859287548650222 39.878051910682174 24.63072438002629 3 19.48597636898878 23.534864809096863 30.15785639772126 26.8213024241931 4 13.047857972372109 16.9556573957977 36.72617578259153 33.27030884923865 5 13.578262029467087 37.20096445106323 34.42577002434766 14.795003495122025 6 32.38003900346979 36.358696845108945 16.79779824568809 14.463465905733171 7 28.346294494649786 33.16123450544916 20.17817952556375 18.314291474337306 8 26.75743378365692 34.528267862726835 19.348977029512255 19.365321324103984 9 26.042065564846578 16.861109478935138 20.25798286012612 36.838842096092165 10-11 25.086710726397982 26.395788582178735 26.902680358479753 21.614820332943523 12-13 26.188783713005474 23.667241530107052 27.433345614046345 22.710629142841128 14-15 22.561521475657678 25.180588954179655 26.045247955024898 26.21264161513776 16-17 21.96885031024575 28.389077499048977 27.078952136093783 22.563120054611492 18-19 22.07948379131613 26.432664490357727 29.662362563794137 21.825489154532004 20-21 22.294356871666775 25.629060106717255 30.201436336885056 21.875146684730908 22-23 22.16108693427815 25.54983768859646 30.207244630114715 22.08183074701067 24-25 22.13022593141601 25.789110303976674 30.100983358100624 21.979680406506688 26-27 21.52462179745049 26.226884713453032 29.860311033155227 22.388182455941248 28-29 21.770680457127575 26.789331213524992 29.56992497148187 21.87006335786557 30-31 21.884207901207635 26.128350175422366 29.479838374028272 22.507603549341727 32-33 21.474917646725093 26.14655902698101 29.514664024472715 22.863859301821186 34-35 22.41133978069426 26.36365263689046 29.23877279030856 21.986234792106725 36-37 22.56074538474216 25.740210898303573 29.339342016745423 22.359701700208845 38-39 21.897337106577716 26.233871685484754 29.19218805334805 22.676603154589476 40-41 22.530756038265924 26.05708813845854 29.266186977459785 22.14596884581575 42-43 22.169684423631978 26.171794717206982 28.98119058994155 22.677330269219485 44-45 22.195626517960374 26.32920686206319 29.265625904992714 22.209540714983717 46-47 22.7490442723798 26.730284471749954 28.340597499244822 22.180073756625422 48-49 22.328447148085587 26.81486076600814 28.180083977369204 22.67660810853707 50-51 22.513636825659688 27.034852798601143 28.255691874096467 22.1958185016427 52-53 22.98567884258276 26.582734578221668 28.236173307313077 22.19541327188249 54-55 22.23960020031858 26.927889026479402 28.420974962541575 22.411535810660446 56-57 22.464874177847914 27.006165908493042 28.15608977595184 22.372870137707203 58-59 22.489019117727015 26.56086905574374 27.99441998620193 22.955691840327315 60-61 21.93452972917482 26.999260117996155 28.407821879908134 22.65838827292089 62-63 22.09881662779275 26.98693437945647 28.572682854019693 22.341566138731082 64-65 21.744730240237086 27.071137746713163 28.310718698618132 22.873413314431616 66-67 21.68329154109437 27.074211771245192 28.28071889967684 22.96177778798359 68-69 22.472784127796196 26.580774682548324 28.390280883841516 22.55616030581396 70-71 22.179727503247747 26.92890210087992 27.834556845799607 23.05681355007272 72-73 22.15379279859439 27.086806630774568 27.88760817524026 22.87179239539078 74-75 22.2464384525221 27.502707441335396 27.597007783450618 22.653846322691887 76-77 22.269452125987282 26.905466313081188 28.0310088566341 22.794072704297427 78-79 22.33670338160858 27.262304886091606 27.82516902302144 22.575822709278377 80-81 22.60490997633791 27.35936219408046 27.43698073500048 22.598747094581157 82-83 22.42826304072348 27.50930560632705 27.614143001989866 22.448288350959604 84-85 22.350486650994863 27.72387617407655 27.5634659020106 22.362171272917987 86-87 22.678469065136913 27.65455612259974 27.106225807404783 22.560749004858568 88-89 22.332399784485123 27.83601553449597 26.883167602663782 22.948417078355124 90-91 22.24956883494762 27.821923345658444 27.104451865702767 22.82405595369117 92-93 22.534536355849454 27.352429123581878 27.354235784826308 22.75879873574236 94-95 22.14711579909968 27.172814994329308 27.57379031597659 23.10627889059442 96-97 22.541041457156616 26.649357675108753 27.659615422281085 23.149985445453545 98-99 22.217415735649094 27.237532063880572 27.03954308885764 23.505509111612696 100-101 22.343514586349063 26.788267838439715 27.431315673100578 23.43690190211064 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 739.0 1 518.5 2 465.5 3 599.5 4 468.0 5 460.5 6 995.5 7 2322.5 8 3733.0 9 4375.5 10 3707.0 11 2770.5 12 2673.0 13 2806.5 14 2994.5 15 3254.5 16 3521.0 17 3822.5 18 4306.0 19 4837.0 20 5381.5 21 6465.0 22 8002.0 23 9841.5 24 12429.5 25 15796.5 26 20126.0 27 25187.0 28 29613.0 29 33344.0 30 37315.5 31 43935.0 32 54621.0 33 58500.0 34 56744.0 35 58677.0 36 62473.5 37 67230.0 38 72199.0 39 75985.0 40 79888.5 41 86285.5 42 90317.0 43 92431.0 44 95738.5 45 102048.0 46 105616.0 47 107759.5 48 110069.0 49 106764.5 50 103889.5 51 101640.5 52 99394.0 53 101669.5 54 105370.0 55 100229.5 56 90936.0 57 83672.5 58 78781.0 59 69541.0 60 54194.0 61 45336.5 62 40004.5 63 29468.5 64 19640.5 65 14780.0 66 11204.5 67 8616.0 68 6534.5 69 4836.0 70 3574.0 71 2395.5 72 1598.0 73 1237.0 74 932.5 75 702.5 76 509.0 77 329.0 78 221.0 79 120.0 80 60.0 81 30.5 82 19.5 83 11.5 84 5.0 85 4.5 86 4.0 87 2.0 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01185297048610349 3 0.005875831523025662 4 0.0 5 4.3899890689272185E-4 6 2.701531734724442E-4 7 0.0 8 1.6884573342027762E-4 9 3.376914668405552E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.065372002608329E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0017728802009129153 26-27 0.021409638997691202 28-29 3.0392232015649976E-4 30-31 5.065372002608329E-5 32-33 0.0036301832685359694 34-35 0.01637803614176693 36-37 0.007935749470753048 38-39 0.02068360234398401 40-41 0.023148750051920065 42-43 0.018826299276360956 44-45 0.009404707351509464 46-47 0.0019417259343331927 48-49 3.0392232015649976E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.532686001304164E-4 68-69 1.857303067623054E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.0261488010433316E-4 82-83 0.0010299589738636936 84-85 0.004254912482190997 86-87 6.922675070231382E-4 88-89 8.442286671013881E-5 90-91 0.0040522976020866625 92-93 6.584983603390828E-4 94-95 1.1819201339419433E-4 96-97 3.0392232015649976E-4 98-99 2.3638402678838867E-4 100-101 0.0024482631345940256 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2961283.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.30310274685124 #Duplication Level Percentage of deduplicated Percentage of total 1 70.60557919266692 33.39862967051665 2 15.443434592591625 14.610447465952767 3 5.753343638095351 8.164530157523018 4 2.7486610402109584 5.200807824054638 5 1.4978829812310808 3.542725628196682 6 0.9471175925971618 2.688096047758436 7 0.6061763700333686 2.0071816180081226 8 0.41684871561650944 1.5774590099760573 9 0.32153001987167845 1.3688430809568413 >10 1.4992025111430751 12.574544914692835 >50 0.10168837913817337 3.3201737162372034 >100 0.04924566502956082 4.512508425188113 >500 0.00457319471996127 1.424407280683419 >1k 0.00435882621720271 4.211670249384705 >5k 2.1436850248537917E-4 0.5947621202562821 >10k+ 1.429123349902528E-4 0.8032127906143459 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12794 0.43204246267580637 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10969 0.37041376997740505 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6982 0.2357761821480757 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5351 0.18069870390638113 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5263 0.17772701899818424 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4913 0.1659078176587648 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4719 0.15935660320205802 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4617 0.15591215024028438 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3828 0.12926829350656455 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3643 0.12302100137001429 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3615 0.12207546526286073 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3456 0.1167061709400959 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3104 0.10481943130730834 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3020 0.10198282298584768 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 3.376914668405553E-5 6 0.0 0.0 0.0 0.0 3.376914668405553E-5 7 0.0 0.0 0.0 0.0 3.376914668405553E-5 8 0.0 0.0 0.0 0.0 3.376914668405553E-5 9 0.0 0.0 0.0 0.0 3.376914668405553E-5 10-11 0.0 0.0 0.0 0.0 3.376914668405553E-5 12-13 0.0 0.0 0.0 0.0 2.363840267883887E-4 14-15 0.0 0.0 0.0 0.0 3.3769146684055524E-4 16-17 0.0 0.0 0.0 0.0 4.052297602086663E-4 18-19 0.0 0.0 0.0 0.0 4.052297602086663E-4 20-21 0.0 0.0 0.0 0.0 5.065372002608329E-4 22-23 0.0 0.0 0.0 5.065372002608329E-5 6.078446403129994E-4 24-25 0.0 0.0 0.0 3.039223201564997E-4 6.078446403129994E-4 26-27 0.0 0.0 0.0 0.0010130744005216658 6.922675070231382E-4 28-29 0.0 0.0 0.0 0.0030054540548809417 7.429212270492216E-4 30-31 0.0 0.0 0.0 0.010333358885320991 7.429212270492216E-4 32-33 0.0 0.0 0.0 0.027420547107453085 7.429212270492216E-4 34-35 0.0 0.0 0.0 0.05220710077354984 7.429212270492216E-4 36-37 0.0 0.0 0.0 0.09274696136775851 7.429212270492216E-4 38-39 0.0 0.0 0.0 0.15344700253234833 8.779978137854437E-4 40-41 0.0 0.0 0.0 0.21708495945845097 8.779978137854437E-4 42-43 0.0 0.0 0.0 0.2838634470261707 8.948823871274714E-4 44-45 0.0 0.0 0.0 0.36512889852135033 9.624206804955825E-4 46-47 0.0 0.0 0.0 0.4598682395434681 0.0010130744005216658 48-49 0.0 0.0 0.0 0.5576468037671509 0.0010299589738636936 50-51 0.0 0.0 0.0 0.6625844270878535 0.0011143818405738323 52-53 0.0 0.0 0.0 0.7616293343121883 0.0011143818405738323 54-55 0.0 0.0 0.0 0.8686099910072762 0.001148150987257888 56-57 0.0 0.0 0.0 0.991310185483792 0.0011819201339419434 58-59 0.0 0.0 0.0 1.1207810938704608 0.0011819201339419434 60-61 0.0 0.0 0.0 1.2623075876233376 0.0011819201339419434 62-63 0.0 0.0 0.0 1.398701171080238 0.001350765867362221 64-65 0.0 0.0 0.0 1.5398730887929322 0.0013845350140462766 66-67 0.0 0.0 0.0 1.7013909173827697 0.0013845350140462766 68-69 0.0 0.0 0.0 1.8837780786233536 0.0013845350140462766 70-71 0.0 0.0 0.0 2.0695421545323427 0.0014183041607303323 72-73 0.0 0.0 0.0 2.2622120209382217 0.001502727027440471 74-75 0.0 0.0 0.0 2.4526531236629525 0.0015533807474665542 76-77 0.0 0.0 0.0 2.6610256432769175 0.0015533807474665542 78-79 0.0 0.0 0.0 2.897291478051912 0.0015533807474665542 80-81 0.0 0.0 0.0 3.140530641617164 0.0015871498941506098 82-83 0.0 0.0 0.0 3.395183776761627 0.0017391110542288595 84-85 0.0 0.0 0.0 3.6565907412429004 0.0017897647742549428 86-87 0.0 5.065372002608329E-5 0.0 3.9247515350609854 0.0018910722143071093 88-89 0.0 6.753829336811106E-5 0.0 4.2119750121822195 0.001992379654359276 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7670 0.0 46.88563 1 GTATCAA 12535 0.0 36.344067 1 TCAACGC 15645 0.0 28.931341 4 ATCAACG 15570 0.0 28.889141 3 TATCAAC 15615 0.0 28.810263 2 CAACGCA 15945 0.0 28.38701 5 AACGCAG 16420 0.0 27.565823 6 TAAGGTG 2500 0.0 27.167349 5 AGGTAAG 2280 0.0 26.461113 2 ACGCAGA 18375 0.0 24.348644 7 GTAAGGT 2685 0.0 24.234135 4 CGCAGAG 18470 0.0 24.120548 8 GTGTAAG 1315 0.0 23.84281 1 AAGGTAA 2380 0.0 23.752497 1 GGTAAGG 2660 0.0 23.748886 3 GAGTACT 12970 0.0 22.337835 12-13 GTACATG 9050 0.0 21.994022 1 GCAGAGT 20060 0.0 21.971935 9 GTACTTT 13315 0.0 21.652037 14-15 TACATGG 9050 0.0 21.31163 2 >>END_MODULE