FastQCFastQC Report
Thu 26 May 2016
SRR938237_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938237_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2989912
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139820.4676391813538325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111230.372017637977305No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71010.23749862872218314No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA54360.1818113710370071No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA49470.16545637463577523No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC47910.16023882977157855No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA47380.15846620234976816No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA43770.14639226840121047No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA39060.13063929640738592No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG38510.12879977738475246No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA35350.11823090445471304No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA34890.11669239763578325No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT31300.1046853552880486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78150.045.1687361
GTATCAA128050.037.638651
ATCAACG159300.029.9796853
TATCAAC160150.029.8799082
TCAACGC160850.029.6317044
CAACGCA163900.029.080295
TAAGGTG24650.028.5313015
AACGCAG168700.028.2810426
AGGTAAG23100.027.771452
AAGGTAA23650.026.8622861
GTAAGGT26100.026.5820884
GGTAAGG25550.025.6663993
ACGCAGA186800.025.4250477
CGCAGAG186800.025.3741478
GAGTACT127100.023.18710712-13
GCAGAGT202350.022.8599629
GTACATG93250.022.589791
TACATGG91300.022.3286912
GGTGTGC34850.021.8268158
GTACTTT132950.021.77775414-15
Produced by FastQC (version 0.11.5)