##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938237_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2989912 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.92637743184415 33.0 31.0 34.0 30.0 34.0 2 32.1169291939027 34.0 31.0 34.0 30.0 34.0 3 32.18715233090472 34.0 31.0 34.0 30.0 34.0 4 35.7055675886113 37.0 35.0 37.0 35.0 37.0 5 35.72792042040033 37.0 35.0 37.0 35.0 37.0 6 35.75635403316218 37.0 36.0 37.0 35.0 37.0 7 35.70214742106122 37.0 35.0 37.0 35.0 37.0 8 35.68124379580402 37.0 35.0 37.0 33.0 37.0 9 37.35485760116017 39.0 37.0 39.0 35.0 39.0 10-11 37.27733608882134 39.0 37.0 39.0 34.0 39.0 12-13 37.1859640016161 39.0 37.0 39.0 34.0 39.0 14-15 38.44770297587354 40.0 38.0 41.0 34.0 41.0 16-17 38.370663584747646 40.0 38.0 41.0 34.0 41.0 18-19 38.322755485780185 40.0 38.0 41.0 34.0 41.0 20-21 38.2112563848033 40.0 38.0 41.0 33.5 41.0 22-23 38.06704979945898 40.0 38.0 41.0 33.0 41.0 24-25 38.01169783592293 40.0 38.0 41.0 33.0 41.0 26-27 38.018085649343526 40.0 38.0 41.0 33.0 41.0 28-29 37.92758148065896 40.0 38.0 41.0 33.0 41.0 30-31 37.72162525184687 40.0 37.5 41.0 33.0 41.0 32-33 37.4819003034203 40.0 37.0 41.0 32.0 41.0 34-35 37.26834435260971 40.0 37.0 41.0 31.5 41.0 36-37 37.03070709104482 40.0 36.5 41.0 31.0 41.0 38-39 36.7721799170009 40.0 36.0 41.0 30.0 41.0 40-41 36.5335742322851 39.0 35.5 41.0 30.0 41.0 42-43 36.23288595115843 39.0 35.0 41.0 30.0 41.0 44-45 35.987736762821115 39.0 35.0 41.0 29.0 41.0 46-47 35.818180267512886 39.0 35.0 41.0 27.5 41.0 48-49 35.539614376610416 39.0 35.0 41.0 26.5 41.0 50-51 34.876557570925165 38.5 34.5 40.0 25.5 40.5 52-53 34.93703192602324 38.5 34.5 40.0 26.0 41.0 54-55 35.05965326069797 39.0 35.0 40.0 26.0 41.0 56-57 34.83554181527751 38.5 35.0 40.0 25.0 41.0 58-59 34.555864520427356 38.0 34.0 40.0 24.5 41.0 60-61 34.167790557046494 38.0 34.0 40.0 23.0 41.0 62-63 33.7468706436845 37.0 34.0 40.0 22.0 41.0 64-65 33.31511161532514 36.5 33.0 39.5 20.5 41.0 66-67 32.88266276733228 36.0 33.0 39.0 19.5 41.0 68-69 32.394312106844616 35.5 32.0 39.0 17.5 40.5 70-71 31.899698051313884 35.0 31.5 38.0 12.5 40.0 72-73 31.420888139851606 35.0 31.0 37.5 8.0 40.0 74-75 30.90715462528663 35.0 31.0 37.0 7.0 39.0 76-77 30.380114364569927 35.0 30.0 36.0 2.0 39.0 78-79 29.82012196345578 34.0 29.5 36.0 2.0 38.5 80-81 29.297827160130467 34.0 29.0 35.0 2.0 37.0 82-83 28.760445457926522 34.0 29.0 35.0 2.0 37.0 84-85 28.27716835813228 34.0 27.5 35.0 2.0 36.0 86-87 27.793503454282266 33.5 26.5 35.0 2.0 36.0 88-89 27.23275685036884 33.0 26.0 35.0 2.0 35.5 90-91 26.626787678031995 33.0 25.0 35.0 2.0 35.0 92-93 26.02558570285681 32.0 23.5 35.0 2.0 35.0 94-95 25.424121679835395 32.0 21.0 34.5 2.0 35.0 96-97 24.580961078453143 32.0 12.5 34.0 2.0 35.0 98-99 23.490444033135425 31.0 2.0 34.0 2.0 35.0 100-101 20.563266912203435 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4349883948757842 1101 2 0.35795831051356686 1101 3 0.2962160315701965 1101 4 0.2501058680598618 1101 5 0.24292484570445083 1101 6 0.21511268551324747 1101 7 0.3234829629415188 1101 8 0.21639207670757088 1101 9 0.20279279900570657 1101 10-11 0.36844010019640194 1101 12-13 0.1265360990014699 1101 14-15 0.2885571932660156 1101 16-17 0.43992207618857293 1101 18-19 0.40323221974137624 1101 20-21 0.5141970874130877 1101 22-23 0.4517483412547847 1101 24-25 0.527657556108224 1101 26-27 0.4821236725751561 1101 28-29 0.49053327538783265 1101 30-31 0.5216174536272575 1101 32-33 0.5294580165211968 1101 34-35 0.4225733837504819 1101 36-37 0.5604773577410569 1101 38-39 0.6720810754549191 1101 40-41 0.6974464004191603 1101 42-43 0.7096814342426185 1101 44-45 0.6025173042747554 1101 46-47 0.6689794921186376 1101 48-49 0.6327066525467586 1101 50-51 0.7417347826952394 1101 52-53 0.6586407702717523 1101 54-55 0.7321416084953114 1101 56-57 0.7596109134656359 1101 58-59 0.6548820048913839 1101 60-61 0.5915495461001186 1101 62-63 0.6420410458992762 1101 64-65 0.5859972939817197 1101 66-67 0.6028529576706774 1101 68-69 0.5609298303533805 1101 70-71 0.7428285642078123 1101 72-73 0.6950429467168533 1101 74-75 0.715784433664794 1101 76-77 0.7383005376848786 1101 78-79 0.6798119883758496 1101 80-81 0.8986912234547653 1101 82-83 0.8019552498840561 1101 84-85 0.9455444493181275 1101 86-87 1.0417251603578421 1101 88-89 1.06884793511448 1101 90-91 0.9368223761101646 1101 92-93 1.165194856452473 1101 94-95 1.352184404787451 1101 96-97 1.509784499066356 1101 98-99 1.6301707733826838 1101 100-101 1.5906332710501303 1102 1 0.4320461343994815 1102 2 0.2823866238300141 1102 3 0.33649126143646413 1102 4 0.27106983766596926 1102 5 0.11525651265746717 1102 6 0.23522771563556688 1102 7 0.09823175888013225 1102 8 0.14438200083369424 1102 9 0.31506909853295184 1102 10-11 0.28237977796795377 1102 12-13 0.21958690512680334 1102 14-15 0.28094223242225524 1102 16-17 0.32331302619162017 1102 18-19 0.2963018696352435 1102 20-21 0.3625481191094124 1102 22-23 0.4910386378224558 1102 24-25 0.3783909389526343 1102 26-27 0.38969069265485246 1102 28-29 0.45919962152568417 1102 30-31 0.34501441700938074 1102 32-33 0.46199725589801943 1102 34-35 0.4723598672261673 1102 36-37 0.4902679009403954 1102 38-39 0.4407422257007241 1102 40-41 0.5472305284237748 1102 42-43 0.5311737914317902 1102 44-45 0.5829713975292421 1102 46-47 0.6354298249328494 1102 48-49 0.5362482418523697 1102 50-51 0.5562454543647348 1102 52-53 0.5625507136850345 1102 54-55 0.4761231028061559 1102 56-57 0.5064091355006539 1102 58-59 0.46445034403488705 1102 60-61 0.4943091190116533 1102 62-63 0.4841656669455503 1102 64-65 0.5456074080973607 1102 66-67 0.568583139845579 1102 68-69 0.5016319547111721 1102 70-71 0.5324420452615115 1102 72-73 0.5661711065120016 1102 74-75 0.40971015873421024 1102 76-77 0.32098588554638496 1102 78-79 0.23450089992652323 1102 80-81 0.4334721785012938 1102 82-83 0.5958243743693181 1102 84-85 0.6272742379908038 1102 86-87 0.5540770427948942 1102 88-89 0.5484602245769317 1102 90-91 0.675143881283887 1102 92-93 0.7704318306974756 1102 94-95 0.7086041719006388 1102 96-97 0.7320415530094131 1102 98-99 0.8539105963621054 1102 100-101 0.6943235321471697 1103 1 0.35133321170555476 1103 2 0.34355516682710885 1103 3 0.2997842690226804 1103 4 0.1980391559244694 1103 5 0.2596024830226398 1103 6 0.22179619146912444 1103 7 0.33141196828673714 1103 8 0.3268580782619708 1103 9 0.30904884608170136 1103 10-11 0.31163353789632 1103 12-13 0.3797929285624875 1103 14-15 0.34857207634266274 1103 16-17 0.4093482582432273 1103 18-19 0.40319758949198814 1103 20-21 0.415260711904871 1103 22-23 0.4067240604272584 1103 24-25 0.5488318387862279 1103 26-27 0.41901982412347394 1103 28-29 0.4755857626856681 1103 30-31 0.49699409063351396 1103 32-33 0.5298511755929951 1103 34-35 0.5587140142853784 1103 36-37 0.5623141488122627 1103 38-39 0.5456373112789876 1103 40-41 0.5855637308763377 1103 42-43 0.5136375437059257 1103 44-45 0.48420047978931535 1103 46-47 0.5202576391095093 1103 48-49 0.47405008152302486 1103 50-51 0.5119259363848059 1103 52-53 0.5803638498790349 1103 54-55 0.6538432755056363 1103 56-57 0.5672282465663443 1103 58-59 0.7224848778803263 1103 60-61 0.6186295419762118 1103 62-63 0.5928939149229109 1103 64-65 0.584414017484292 1103 66-67 0.5558551491574519 1103 68-69 0.7139298409275057 1103 70-71 0.9694386064514333 1103 72-73 1.0435833213579855 1103 74-75 0.8717042897509941 1103 76-77 0.7542013735692166 1103 78-79 0.8459648848922008 1103 80-81 0.8523987088756932 1103 82-83 0.8139230411085911 1103 84-85 0.8959098513198072 1103 86-87 0.8612079139649396 1103 88-89 1.0403516016912668 1103 90-91 0.956587855302935 1103 92-93 1.0978675054286349 1103 94-95 1.0414237817601837 1103 96-97 1.0574007615372132 1103 98-99 0.9981639636481461 1103 100-101 0.8515672435891375 1104 1 0.2789388854425958 1104 2 0.23601850077220377 1104 3 0.14379972633319937 1104 4 0.1552253070592826 1104 5 0.23817339612710242 1104 6 0.20197452419985495 1104 7 0.21964794979628266 1104 8 0.1878869806778951 1104 9 0.13354814842202245 1104 10-11 0.25677942734555614 1104 12-13 0.17478117146747252 1104 14-15 0.29747807489715683 1104 16-17 0.35099449331699617 1104 18-19 0.43550441615332147 1104 20-21 0.4143551646587085 1104 22-23 0.410469150342756 1104 24-25 0.5181522131230452 1104 26-27 0.48430421248162503 1104 28-29 0.6094626075275471 1104 30-31 0.5122883879936921 1104 32-33 0.39625529949618254 1104 34-35 0.46696324931408384 1104 36-37 0.4948933722908535 1104 38-39 0.5145754289202742 1104 40-41 0.5385243611841091 1104 42-43 0.49087467949515684 1104 44-45 0.45607429954658585 1104 46-47 0.6278084085499245 1104 48-49 0.5298240258663043 1104 50-51 0.4897916802138482 1104 52-53 0.5612264715482098 1104 54-55 0.519875766042631 1104 56-57 0.5337127414508416 1104 58-59 0.5748572321676235 1104 60-61 0.710900117441966 1104 62-63 0.6464558256715662 1104 64-65 0.7179899290483718 1104 66-67 0.7154325750954413 1104 68-69 0.684261079999672 1104 70-71 0.76895568455231 1104 72-73 0.8474449748389077 1104 74-75 0.7952789594845058 1104 76-77 0.7525776526272239 1104 78-79 0.7231463764149701 1104 80-81 0.8929369673310426 1104 82-83 0.9067888893393139 1104 84-85 0.6665963125459378 1104 86-87 0.7111431299720259 1104 88-89 0.7245288120123057 1104 90-91 0.7732914655131999 1104 92-93 0.6839311259838219 1104 94-95 0.8986350625637165 1104 96-97 0.9450310301132951 1104 98-99 1.0958835744240147 1104 100-101 1.0479045027921465 1105 1 0.31472881238954287 1105 2 0.29903159855839334 1105 3 0.19057314963436056 1105 4 0.16425026236739626 1105 5 0.15415482537420644 1105 6 0.19129531059382288 1105 7 0.19681563512875044 1105 8 0.3181647746795093 1105 9 0.2921826438016595 1105 10-11 0.3314365795934364 1105 12-13 0.21455861102606377 1105 14-15 0.262302668547413 1105 16-17 0.32150151289209106 1105 18-19 0.2344887278982739 1105 20-21 0.3071967898973398 1105 22-23 0.2366165665733746 1105 24-25 0.2692688517892847 1105 26-27 0.17391347770234233 1105 28-29 0.26451986238639336 1105 30-31 0.3323546728467761 1105 32-33 0.3124965200170067 1105 34-35 0.4386032840827099 1105 36-37 0.40052483629513347 1105 38-39 0.39809634792975856 1105 40-41 0.5716517854541223 1105 42-43 0.46518981426938666 1105 44-45 0.41163430234090015 1105 46-47 0.4889576742974526 1105 48-49 0.4270172837759958 1105 50-51 0.30075570796919493 1105 52-53 0.3445397636768277 1105 54-55 0.36514478199657674 1105 56-57 0.5454813949452841 1105 58-59 0.470631744565118 1105 60-61 0.43550315843710763 1105 62-63 0.5434908153245743 1105 64-65 0.4763732795026172 1105 66-67 0.5843059501832002 1105 68-69 0.7141516779261536 1105 70-71 0.759519197420488 1105 72-73 0.7111273916020551 1105 74-75 0.7113595068040794 1105 76-77 0.6665050301726012 1105 78-79 0.6768727807594423 1105 80-81 0.7288723361636436 1105 82-83 0.7568121943973729 1105 84-85 0.8897546689839189 1105 86-87 1.0204881146891758 1105 88-89 0.8715094613702412 1105 90-91 0.9547098903661286 1105 92-93 1.1416718981542608 1105 94-95 1.3731704215475027 1105 96-97 1.2031430799162592 1105 98-99 1.321413893331112 1105 100-101 1.2028233588172768 1106 1 0.10836458561145079 1106 2 0.16981600676513153 1106 3 0.10421668545312457 1106 4 0.1328406343214823 1106 5 0.06663738985393053 1106 6 0.13590554332838423 1106 7 0.1783095601248661 1106 8 0.06656941768275004 1106 9 0.1265566135209184 1106 10-11 -0.4250886879901188 1106 12-13 -0.1360164716996266 1106 14-15 0.016125912893897976 1106 16-17 0.19664598650754073 1106 18-19 0.23240010418243173 1106 20-21 0.09340274350039834 1106 22-23 -0.0031823446783079135 1106 24-25 0.06035752291870722 1106 26-27 0.06316911743845566 1106 28-29 -0.09597772668774951 1106 30-31 0.02692449376675654 1106 32-33 0.06656085694898906 1106 34-35 -0.0704319265714517 1106 36-37 -0.14606772550517633 1106 38-39 0.026187410688805812 1106 40-41 0.08113152606870955 1106 42-43 -0.0162911546879414 1106 44-45 0.17787885942630766 1106 46-47 0.15904047165507507 1106 48-49 0.2540931444153287 1106 50-51 0.2363203100864908 1106 52-53 0.2954304775600747 1106 54-55 0.07343295296776375 1106 56-57 0.23556114901209924 1106 58-59 0.1796538889163415 1106 60-61 -0.04605163237524579 1106 62-63 0.07169853756474964 1106 64-65 0.19409559685831113 1106 66-67 0.004813429952484682 1106 68-69 -0.3124010344067898 1106 70-71 -0.06761102344965408 1106 72-73 0.2514454265183055 1106 74-75 0.30677676292456724 1106 76-77 0.32919274112172303 1106 78-79 0.4343709641574094 1106 80-81 0.39705682574533796 1106 82-83 0.42218351446886615 1106 84-85 0.3443966247172696 1106 86-87 0.35880717065154144 1106 88-89 0.32104822638959973 1106 90-91 0.3621290694935624 1106 92-93 0.4648516298374581 1106 94-95 0.38756182403043127 1106 96-97 0.5578897866014678 1106 98-99 0.553820529910368 1106 100-101 0.481751547077895 1107 1 0.32176338070612687 1107 2 0.2524085277816539 1107 3 0.19228158985472277 1107 4 0.1541678366976882 1107 5 0.21254037564965955 1107 6 0.23988124776527542 1107 7 0.171518103693451 1107 8 0.21536713810942842 1107 9 0.26985306728456493 1107 10-11 0.2154455791308365 1107 12-13 0.1570787969043579 1107 14-15 0.25042664348328714 1107 16-17 0.3322657376282052 1107 18-19 0.35785861309108924 1107 20-21 0.3364458121438645 1107 22-23 0.36550707921058745 1107 24-25 0.4412651510449308 1107 26-27 0.3922242771472284 1107 28-29 0.4602598009876786 1107 30-31 0.42853933157927315 1107 32-33 0.48679789938535833 1107 34-35 0.48792148501630805 1107 36-37 0.41771805299894993 1107 38-39 0.5078807164902486 1107 40-41 0.5514375207667896 1107 42-43 0.4850923543257437 1107 44-45 0.46819839250449036 1107 46-47 0.4976517951603938 1107 48-49 0.3808079183695483 1107 50-51 0.42490359055467053 1107 52-53 0.4786176264969626 1107 54-55 0.5302121733923428 1107 56-57 0.4964354930991206 1107 58-59 0.5865134048536049 1107 60-61 0.5431837799939458 1107 62-63 0.5798811176922101 1107 64-65 0.6158881876148214 1107 66-67 0.5977400727282998 1107 68-69 0.8372003763575435 1107 70-71 0.8879471170134039 1107 72-73 0.7933780118972749 1107 74-75 0.7162992645735393 1107 76-77 0.8229618828577472 1107 78-79 0.648423834209968 1107 80-81 0.8658955499236889 1107 82-83 0.7624100959071072 1107 84-85 0.8015618307653227 1107 86-87 0.9655089313761671 1107 88-89 0.9359129266126835 1107 90-91 0.877557781362384 1107 92-93 0.9942119545065076 1107 94-95 0.8855326949820466 1107 96-97 0.9954188274245617 1107 98-99 1.0184264884780916 1107 100-101 0.8327384099127819 1108 1 0.2860905983271529 1108 2 0.24064172442989928 1108 3 0.1507853743569072 1108 4 0.21731877917649456 1108 5 0.25171524072040796 1108 6 0.28739133706980624 1108 7 0.28393645731344463 1108 8 0.18014979260949815 1108 9 0.2425070000873717 1108 10-11 0.33236360049261293 1108 12-13 0.1554217603235415 1108 14-15 0.23356980625739965 1108 16-17 0.2876639207430074 1108 18-19 0.4170639891911847 1108 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64-65 -0.7106714042756579 2316 66-67 -0.8172962436099596 2316 68-69 -0.914263398050565 2316 70-71 -0.8989866078934483 2316 72-73 -0.969680700999028 2316 74-75 -0.8281654297535646 2316 76-77 -0.9399605325613081 2316 78-79 -0.8769095697038694 2316 80-81 -0.9877233616181833 2316 82-83 -1.0500670395683152 2316 84-85 -1.1647942668564113 2316 86-87 -1.1004604710857286 2316 88-89 -1.1904544075438928 2316 90-91 -1.0819285942279144 2316 92-93 -1.26484382069874 2316 94-95 -1.3921159867046278 2316 96-97 -1.5280489825276398 2316 98-99 -1.4945888974786428 2316 100-101 -1.2564480044671384 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7875.0 3 2176.0 4 2104.0 5 2310.0 6 2723.0 7 3455.0 8 4289.0 9 5535.0 10 6823.0 11 8668.0 12 11335.0 13 13917.0 14 16736.0 15 20199.0 16 25304.0 17 33118.0 18 29500.0 19 24001.0 20 22650.0 21 23077.0 22 24467.0 23 26854.0 24 29818.0 25 32857.0 26 36446.0 27 42980.0 28 50517.0 29 59862.0 30 71211.0 31 87962.0 32 110184.0 33 143892.0 34 198857.0 35 295704.0 36 444036.0 37 592898.0 38 427225.0 39 48344.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.664981550448104 18.42702989610635 14.879377881849587 23.02861067159596 2 15.455229002358772 19.55087288629874 40.39249516215765 24.601402949184838 3 19.255077843421645 23.547258563579547 30.033351426856097 27.16431216614271 4 12.83972425130642 16.659950775417695 36.94407313672506 33.55625183655083 5 13.58023203657957 37.13555997402476 34.39433498691196 14.889873002483714 6 32.25076097703726 36.27068851387571 16.97600733404071 14.502543175046315 7 28.32020718902819 33.1581098218323 20.259334367091807 18.262348622047707 8 26.65701360252153 34.304663152790695 19.580048207317567 19.458275037370207 9 26.220600365754287 16.85956608089093 20.270082604250845 36.64975094910394 10-11 25.034797654796222 26.328030299607097 26.95344252023218 21.683729525364505 12-13 26.22499083422759 23.776231268760537 27.347852363262582 22.650925533749295 14-15 22.69761863409368 25.18436418992699 26.02375375764287 26.09426341833646 16-17 21.991331589298987 28.323066466181917 27.059232801142937 22.626369143376156 18-19 22.137351188210282 26.422357673038494 29.656020482385546 21.78427065636568 20-21 22.409129020974923 25.626327354762914 30.11440563794197 21.850137986320192 22-23 22.204558704719016 25.571377604876112 30.08103538407887 22.143028306326002 24-25 22.10272671154276 25.830781323438636 30.02949596744497 22.036995997573637 26-27 21.49387606200414 26.168049761284394 29.854260483656393 22.483813693055072 28-29 21.80060223564168 26.77368169332838 29.563079614989558 21.86263645604038 30-31 21.88701632425259 26.15543727559278 29.40189591939707 22.555650480757564 32-33 21.446268689437087 26.216278768926653 29.489493851645243 22.847958689991017 34-35 22.432661799203157 26.37151163209085 29.146876244179605 22.04895032452639 36-37 22.57257962489064 25.725930125690216 29.29545334360614 22.406036905812996 38-39 21.90370602355136 26.266017823121878 29.117556718602255 22.71271943472451 40-41 22.518917815621776 26.088500076122983 29.225653611993636 22.166928496261608 42-43 22.16481392316415 26.130710014049914 28.98122919825164 22.723246864534296 44-45 22.188752198717935 26.373023662855 29.201583545259968 22.2366405931671 46-47 22.75107333900517 26.76064230720463 28.298930391293247 22.189353962496952 48-49 22.3437379769492 26.815610310968367 28.136966594737455 22.70368511734498 50-51 22.50951495118198 27.102133861517043 28.161729664483907 22.226621522817062 52-53 22.983238363831465 26.670731411528653 28.15757493641518 22.188455288224702 54-55 22.229325711077912 26.938972276822625 28.39823742034293 22.43346459175653 56-57 22.44170464982812 27.008817044691753 28.17331060449054 22.376167700989594 58-59 22.5248563947531 26.564486792933796 27.962235608614943 22.94842120369816 60-61 21.935870336877617 26.909176129577478 28.482642241416805 22.672311292128104 62-63 22.108168793202136 26.88865018494352 28.69580111136721 22.307379910487136 64-65 21.797545924018586 26.99829822452664 28.391164817974925 22.81299103347985 66-67 21.69960621071139 26.97162255102669 28.347794351806506 22.980976886455416 68-69 22.49720467156459 26.477718911640856 28.51613011135317 22.508946305441377 70-71 22.146956323453608 26.816267538985233 28.003119119967838 23.033657017593313 72-73 22.167995248889596 26.884425893551704 28.131726208898293 22.815852648660403 74-75 22.2318800140919 27.340461586690445 27.829680044938844 22.59797835427881 76-77 22.3431155085282 26.80229135969654 28.05559091397967 22.79900221779559 78-79 22.385802245611664 27.145755688248986 27.86656187524681 22.601880190892544 80-81 22.76019453509394 27.20022372505903 27.446562142328006 22.59301959751902 82-83 22.5074103395333 27.27294319902196 27.694572742763157 22.525073718681583 84-85 22.451657815494755 27.521170639477173 27.65700327547163 22.370168269556444 86-87 22.80321726291819 27.443686860746947 27.16264798693614 22.590447889398728 88-89 22.481314770929757 27.54773673463754 27.022116117353693 22.948832377079007 90-91 22.41057626297366 27.54407208478224 27.180020710671513 22.86533094157258 92-93 22.701925862877705 27.006277238336324 27.52277263763787 22.769024261148104 94-95 22.383090806234936 26.811758491432315 27.726210779637796 23.078939922694957 96-97 22.77730059522714 26.33124730016359 27.763202053665804 23.128250050943468 98-99 22.46918805345449 26.819437601992043 27.19872461333987 23.5126497312136 100-101 22.810002276549316 26.035130556501556 27.700227872702865 23.45463929424626 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 787.0 1 552.5 2 456.0 3 619.0 4 562.5 5 554.5 6 957.0 7 2213.0 8 3849.5 9 4523.0 10 3787.5 11 2907.5 12 2841.5 13 3013.0 14 3122.0 15 3358.5 16 3603.5 17 3921.0 18 4422.5 19 4935.0 20 5513.0 21 6609.0 22 8186.5 23 10163.0 24 12899.5 25 16300.5 26 20619.0 27 25745.0 28 29847.0 29 33328.5 30 37419.5 31 44227.5 32 54663.5 33 58636.5 34 57036.5 35 59027.0 36 63506.5 37 68573.5 38 73421.0 39 76844.0 40 80372.0 41 86332.5 42 90510.0 43 92886.5 44 95541.5 45 101818.0 46 105846.5 47 108096.0 48 110749.0 49 107172.0 50 103433.0 51 101436.5 52 99754.5 53 101768.5 54 105088.0 55 100354.5 56 92055.5 57 84552.0 58 79716.0 59 71041.5 60 55566.5 61 46208.5 62 40798.0 63 30668.0 64 20923.0 65 16062.0 66 12216.0 67 9346.0 68 7155.0 69 5403.0 70 4125.0 71 2805.5 72 1857.5 73 1470.0 74 1088.5 75 805.0 76 607.0 77 398.5 78 261.5 79 154.5 80 92.0 81 53.0 82 33.5 83 27.5 84 22.5 85 15.0 86 11.5 87 8.5 88 9.0 89 8.5 90 4.0 91 3.0 92 3.0 93 4.0 94 6.0 95 3.0 96 1.0 97 1.0 98 0.0 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5031251755904522 2 0.049934580014395075 3 0.0579950179135707 4 0.0660220100123348 5 0.08200910260904 6 0.11007012915430286 7 0.07053719306788962 8 0.05247646084567038 9 0.05240956924484734 10-11 0.07634003943928785 12-13 0.10893297194031128 14-15 0.05582104088682209 16-17 0.06090480254937269 18-19 0.059650585033940794 20-21 0.07682500354525484 22-23 0.06548687720575054 24-25 0.09110636032097266 26-27 0.06846355344237556 28-29 0.06839666184155253 30-31 0.07289512199690158 32-33 0.07229309758949427 34-35 0.0752697738261193 36-37 0.06990172286007079 38-39 0.04046941849793572 40-41 0.04438257714608323 42-43 0.04284407032715344 44-45 0.022408686275716475 46-47 0.029265075360077487 48-49 0.028830279954727766 50-51 0.026773363229419464 52-53 0.026572688426950358 54-55 0.02576998921707395 56-57 0.025368639612135743 58-59 0.021438758063782478 60-61 0.029231629559665972 62-63 0.020769842055552136 64-65 0.022776590080243166 66-67 0.016589117004112496 68-69 0.01837846732612866 70-71 0.04175708181377913 72-73 0.03802787506789497 74-75 0.03214141419546796 76-77 0.02282675878086044 78-79 0.016104152898145496 80-81 0.017676105517486804 82-83 0.02262608397839134 84-85 0.01911427493518204 86-87 0.009699282119339967 88-89 0.04195775661624824 90-91 0.05342966615739861 92-93 0.03404782481892443 94-95 0.054198919566863506 96-97 0.04364676953702985 98-99 0.03393076451748413 100-101 0.17162712481170014 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2989912.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.04218364714141 #Duplication Level Percentage of deduplicated Percentage of total 1 72.28334533119471 35.44933096362192 2 14.74971381058958 14.46716346883424 3 5.353418005517571 7.876299268995185 4 2.5675981385496485 5.03682477771241 5 1.3785885332323455 3.3804496010312 6 0.8490189357258917 2.4982645539461834 7 0.590940329537479 2.0286702915975514 8 0.38717560459874506 1.519034968353973 9 0.2909023654469631 1.2839838506674026 >10 1.40107693323976 12.141788186142275 >50 0.09376326068775082 3.1412604170253586 >100 0.045832502260778105 4.30928192788386 >500 0.004381587006624002 1.438370367866077 >1k 0.003970813224298503 4.165431945901514 >5k 1.3692459394132767E-4 0.42093485761770155 >10k+ 1.3692459394132767E-4 0.8429105528030947 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13982 0.4676391813538325 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11123 0.372017637977305 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7101 0.23749862872218314 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5436 0.1818113710370071 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4947 0.16545637463577523 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4791 0.16023882977157855 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4738 0.15846620234976816 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4377 0.14639226840121047 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3906 0.13063929640738592 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3851 0.12879977738475246 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3535 0.11823090445471304 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3489 0.11669239763578325 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3130 0.1046853552880486 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 1.6722900205758566E-5 0.0 3.344580041151713E-5 3.344580041151713E-5 12-13 0.0 3.344580041151713E-5 0.0 3.344580041151713E-5 3.84626704732447E-4 14-15 0.0 3.344580041151713E-5 0.0 3.344580041151713E-5 4.347954053497227E-4 16-17 0.0 3.344580041151713E-5 0.0 8.361450102879282E-5 4.682412057612398E-4 18-19 0.0 3.344580041151713E-5 0.0 1.3378320164606853E-4 4.8496410596699836E-4 20-21 0.0 3.344580041151713E-5 0.0 2.508435030863785E-4 5.518557067900327E-4 22-23 0.0 3.344580041151713E-5 0.0 4.0134960493820555E-4 5.685786069957912E-4 24-25 0.0 3.344580041151713E-5 0.0 5.685786069957912E-4 5.685786069957912E-4 26-27 0.0 3.344580041151713E-5 0.0 0.0011873259146088582 6.689160082303426E-4 28-29 0.0 3.344580041151713E-5 0.0 0.003093736538065334 6.689160082303426E-4 30-31 0.0 3.344580041151713E-5 0.0 0.011204343137858237 6.689160082303426E-4 32-33 0.0 3.344580041151713E-5 0.0 0.026923869331271288 6.689160082303426E-4 34-35 0.0 3.344580041151713E-5 0.0 0.05110518302879817 7.023618086418597E-4 36-37 0.0 3.344580041151713E-5 0.0 0.09147426412549935 7.023618086418597E-4 38-39 0.0 3.344580041151713E-5 0.0 0.15120846366046894 7.69253409464894E-4 40-41 0.0 3.344580041151713E-5 0.0 0.21472203864193995 7.69253409464894E-4 42-43 0.0 3.344580041151713E-5 0.0 0.2803259761491308 7.859763096706525E-4 44-45 0.0 3.344580041151713E-5 0.0 0.3622180184567305 9.030366111109625E-4 46-47 0.0 3.344580041151713E-5 0.0 0.45737132062749675 0.0010200969125512725 48-49 0.0 3.344580041151713E-5 0.0 0.5544143105215137 0.0011706030144030995 50-51 0.0 3.344580041151713E-5 0.0 0.6618422214433066 0.0012040488148146167 52-53 0.0 3.344580041151713E-5 0.0 0.7626813096840308 0.0012876633158434095 54-55 0.0 3.344580041151713E-5 0.0 0.873704644150062 0.001304386216049168 56-57 0.0 3.344580041151713E-5 0.0 0.998240081982346 0.0013211091162549267 58-59 0.0 3.344580041151713E-5 0.0 1.1292640050944645 0.0013378320164606852 60-61 0.0 3.344580041151713E-5 0.0 1.2702046080285974 0.0013378320164606852 62-63 0.0 3.344580041151713E-5 0.0 1.4083357637281633 0.001538506818929788 64-65 0.0 3.344580041151713E-5 0.0 1.5497278849678517 0.001538506818929788 66-67 0.0 5.01687006172757E-5 0.0 1.7150337535017752 0.0015552297191355465 68-69 0.0 6.689160082303426E-5 0.0 1.8927647368885774 0.001571952619341305 70-71 0.0 6.689160082303426E-5 0.0 2.079776929889575 0.001605398419752822 72-73 0.0 6.689160082303426E-5 0.0 2.2729431501662924 0.0016722900205758564 74-75 0.0 6.689160082303426E-5 0.0 2.4682833474697583 0.0017224587211931322 76-77 0.0 6.689160082303426E-5 0.0 2.675647310021165 0.0017559045216046492 78-79 0.0 6.689160082303426E-5 0.0 2.9129118181404667 0.0017726274218104077 80-81 0.0 6.689160082303426E-5 0.0 3.1529690505941312 0.0018562419228392007 82-83 0.0 6.689160082303426E-5 0.0 3.4045316383893574 0.002107085425925579 84-85 0.0 6.689160082303426E-5 0.0 3.663602808376969 0.002107085425925579 86-87 0.0 6.689160082303426E-5 0.0 3.9297979338522335 0.002274314427983165 88-89 0.0 6.689160082303426E-5 0.0 4.209572054294574 0.0024415434300407504 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7815 0.0 45.168736 1 GTATCAA 12805 0.0 37.63865 1 ATCAACG 15930 0.0 29.979685 3 TATCAAC 16015 0.0 29.879908 2 TCAACGC 16085 0.0 29.631704 4 CAACGCA 16390 0.0 29.08029 5 TAAGGTG 2465 0.0 28.531301 5 AACGCAG 16870 0.0 28.281042 6 AGGTAAG 2310 0.0 27.77145 2 AAGGTAA 2365 0.0 26.862286 1 GTAAGGT 2610 0.0 26.582088 4 GGTAAGG 2555 0.0 25.666399 3 ACGCAGA 18680 0.0 25.425047 7 CGCAGAG 18680 0.0 25.374147 8 GAGTACT 12710 0.0 23.187107 12-13 GCAGAGT 20235 0.0 22.859962 9 GTACATG 9325 0.0 22.58979 1 TACATGG 9130 0.0 22.328691 2 GGTGTGC 3485 0.0 21.826815 8 GTACTTT 13295 0.0 21.777754 14-15 >>END_MODULE