FastQCFastQC Report
Thu 26 May 2016
SRR938237_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938237_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2989912
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133390.446133531689227No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110080.36817137092998053No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70730.23656214631066064No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA55060.1841525770658133No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC53190.1778982123888596No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA49720.16629251964606317No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA49630.1659915074423595No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA45520.15224528347322597No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG40200.13445211765429885No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA37100.12408391952672855No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA35720.11946839906993918No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA34940.11685962663784086No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT31480.10528737969545592No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA30380.10160834165018903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77850.046.6193241
GTATCAA127650.036.879421
TATCAAC157850.029.4022922
TCAACGC158900.029.1443164
ATCAACG159550.029.0568083
CAACGCA160900.028.8115675
AGGTAAG23700.027.8610612
AACGCAG166900.027.832726
TAAGGTG25750.027.1152255
GTAAGGT25300.027.0342984
AAGGTAA24200.026.1076341
GTGTAAG12700.025.8093221
ACGCAGA186000.024.9746287
GGTAAGG26750.024.8598863
CGCAGAG186850.024.8355948
GAGTACT129050.022.580312-13
GCAGAGT205400.022.4307829
GTACTTT135600.021.27941714-15
AGAGTAC173500.021.1067210-11
GGTGTGC36650.020.8653168
Produced by FastQC (version 0.11.5)