##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938237_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2989912 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.532028032932075 34.0 31.0 34.0 31.0 34.0 2 32.74831199045323 34.0 31.0 34.0 31.0 34.0 3 32.804896598963445 34.0 31.0 34.0 31.0 34.0 4 36.25986784895341 37.0 35.0 37.0 35.0 37.0 5 36.2233339977899 37.0 37.0 37.0 35.0 37.0 6 36.260770885564526 37.0 37.0 37.0 35.0 37.0 7 36.227161869647 37.0 37.0 37.0 35.0 37.0 8 36.217595367355294 37.0 37.0 37.0 35.0 37.0 9 37.930703311669376 39.0 38.0 39.0 35.0 39.0 10-11 37.91829876598375 39.0 38.0 39.0 35.0 39.0 12-13 37.8683518110232 39.0 38.0 39.0 35.0 39.0 14-15 39.202729712446384 40.5 39.0 41.0 36.0 41.0 16-17 39.164291122949436 40.0 39.0 41.0 36.0 41.0 18-19 39.194956072285734 40.0 39.0 41.0 36.0 41.0 20-21 39.12096994827941 40.0 39.0 41.0 36.0 41.0 22-23 38.98717821795424 40.0 38.5 41.0 35.0 41.0 24-25 38.94724426672089 40.0 38.0 41.0 35.0 41.0 26-27 38.90711599538716 40.0 38.0 41.0 35.0 41.0 28-29 38.85313982485103 40.0 38.0 41.0 35.0 41.0 30-31 38.64487433074953 40.0 38.0 41.0 35.0 41.0 32-33 38.456913280390864 40.0 38.0 41.0 34.0 41.0 34-35 38.25457689055732 40.0 38.0 41.0 34.0 41.0 36-37 38.11531459788783 40.0 38.0 41.0 34.0 41.0 38-39 38.07775947920875 40.0 38.0 41.0 34.0 41.0 40-41 38.00456317777915 40.0 38.0 41.0 33.0 41.0 42-43 37.84059413788767 40.0 37.5 41.0 33.0 41.0 44-45 37.63245958409478 40.0 37.0 41.0 33.0 41.0 46-47 37.303588366480355 40.0 37.0 41.0 32.5 41.0 48-49 36.95227066883574 40.0 36.5 41.0 31.0 41.0 50-51 36.64658391283757 40.0 36.0 41.0 31.0 41.0 52-53 36.225105454608695 39.0 35.0 41.0 30.0 41.0 54-55 35.99351017688815 39.0 35.0 41.0 29.0 41.0 56-57 35.744997846090456 39.0 35.0 40.5 29.0 41.0 58-59 35.40321788734919 38.0 35.0 40.0 28.0 41.0 60-61 35.040915919933425 38.0 34.5 40.0 27.5 41.0 62-63 34.67931949167735 37.5 34.0 40.0 26.5 41.0 64-65 34.291186496458764 37.0 34.0 40.0 26.0 41.0 66-67 33.87980147910707 36.0 34.0 39.0 26.0 41.0 68-69 33.43299200779153 36.0 33.0 39.0 25.0 40.5 70-71 32.91936334581085 35.0 33.0 38.5 23.5 40.0 72-73 32.51335423918831 35.0 32.0 37.5 22.5 40.0 74-75 32.08589199280782 35.0 32.0 37.0 21.5 39.0 76-77 30.795352505358018 34.0 30.5 35.5 20.5 38.5 78-79 31.507939364101688 35.0 31.5 36.0 21.0 38.5 80-81 31.35396208985415 35.0 32.0 36.0 20.0 37.5 82-83 30.99093852929451 35.0 31.0 35.0 19.5 37.0 84-85 30.623823878428528 34.0 31.0 35.0 18.0 36.5 86-87 30.21921498024022 34.0 31.0 35.0 13.0 36.0 88-89 29.77020076176155 34.0 30.0 35.0 7.0 36.0 90-91 29.44011763556921 34.0 30.0 35.0 3.5 35.0 92-93 29.061720880079413 34.0 29.5 35.0 2.0 35.0 94-95 28.668693760886608 34.0 29.0 35.0 2.0 35.0 96-97 28.11721532272522 34.0 29.0 35.0 2.0 35.0 98-99 27.459073042952433 34.0 28.0 35.0 2.0 35.0 100-101 25.039215368211504 31.0 22.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18473667024857576 1101 2 0.1706368882535756 1101 3 0.17450944634718724 1101 4 0.0578953884296638 1101 5 0.13123951920567123 1101 6 0.05578368914606102 1101 7 0.17300908553837502 1101 8 0.1265823439135545 1101 9 0.14052150066942914 1101 10-11 0.20154028434097881 1101 12-13 0.13948727817643913 1101 14-15 0.18466292554466435 1101 16-17 0.18230223006938928 1101 18-19 0.14105789843640792 1101 20-21 0.24393185855933552 1101 22-23 0.31630990947957116 1101 24-25 0.2586978764760559 1101 26-27 0.2692900487594514 1101 28-29 0.2419550675851312 1101 30-31 0.18624102017454192 1101 32-33 0.09603741724173886 1101 34-35 0.15020956014107867 1101 36-37 0.2000103347844444 1101 38-39 0.1807772132342791 1101 40-41 0.303161203901098 1101 42-43 0.21872574052573412 1101 44-45 0.23193226869862116 1101 46-47 0.30498193514315375 1101 48-49 0.25901951080837904 1101 50-51 0.2563732204511382 1101 52-53 0.15087037145512738 1101 54-55 0.1943691991606542 1101 56-57 0.14243142887011828 1101 58-59 0.1721185753199066 1101 60-61 0.19317793336651334 1101 62-63 0.07268195704359215 1101 64-65 0.1821799298032758 1101 66-67 0.042741756357521865 1101 68-69 5.305154344270591E-4 1101 70-71 0.17025960325835854 1101 72-73 0.1391948386470503 1101 74-75 0.28634108189222474 1101 76-77 0.2686534826807545 1101 78-79 0.2796529891685431 1101 80-81 0.2871698349332128 1101 82-83 0.3721561317827593 1101 84-85 0.4528476717687404 1101 86-87 0.4511693372243535 1101 88-89 0.4734404418873517 1101 90-91 0.5504192449328009 1101 92-93 0.6670153790707367 1101 94-95 0.5301253482441588 1101 96-97 0.6359531818124431 1101 98-99 0.7444148683235099 1101 100-101 0.8929885302880756 1102 1 0.14332665755674867 1102 2 0.14571406457028502 1102 3 0.10177499384179356 1102 4 0.04518651356890757 1102 5 0.03701596560346587 1102 6 0.06201100041740659 1102 7 0.07190700219349822 1102 8 0.1360345721891818 1102 9 0.0034440306116252373 1102 10-11 0.03323224691946791 1102 12-13 0.08627677111279297 1102 14-15 0.16193193696731356 1102 16-17 0.14172910239177128 1102 18-19 0.15230645051448732 1102 20-21 0.0680863137250256 1102 22-23 0.1459872515106042 1102 24-25 0.09887493862169094 1102 26-27 0.015461161262287249 1102 28-29 0.013242221677280952 1102 30-31 0.05993678198930752 1102 32-33 -0.07420231428998392 1102 34-35 0.06221324853165555 1102 36-37 0.09294142755675239 1102 38-39 0.11312621368320208 1102 40-41 0.21933573876484047 1102 42-43 0.20480643065104687 1102 44-45 0.10012571938059978 1102 46-47 0.04793249519104137 1102 48-49 0.09042071126727791 1102 50-51 0.12270834301877187 1102 52-53 0.03997659488679517 1102 54-55 0.09925008160324467 1102 56-57 0.12522540551492511 1102 58-59 0.10665885664628405 1102 60-61 0.1671981959599762 1102 62-63 0.14357602458186847 1102 64-65 0.22788812296299454 1102 66-67 0.06248351359435844 1102 68-69 0.22780333717791734 1102 70-71 0.2903853715575977 1102 72-73 0.08658781712636454 1102 74-75 0.16656323052522026 1102 76-77 0.20161913159917688 1102 78-79 0.2967291152222735 1102 80-81 0.2570410738001492 1102 82-83 0.2672478150300961 1102 84-85 0.11003816287060175 1102 86-87 0.07572706289233722 1102 88-89 -0.09262345511482195 1102 90-91 0.003721476810916613 1102 92-93 0.23137225233530856 1102 94-95 0.2184442901111332 1102 96-97 0.30797877608393165 1102 98-99 0.11294757611762662 1102 100-101 0.3964683231174675 1103 1 0.1423389352255029 1103 2 0.15565679403815125 1103 3 0.12151168277397772 1103 4 0.06197792473854946 1103 5 0.10744060728282534 1103 6 0.14202940001784725 1103 7 0.054359771005835 1103 8 0.08336709708711254 1103 9 0.08007307859277546 1103 10-11 0.08312406310919584 1103 12-13 0.10060847876223988 1103 14-15 0.1854209579120507 1103 16-17 0.16019887860142745 1103 18-19 0.1785059530623201 1103 20-21 0.19165677013324967 1103 22-23 0.216604597040714 1103 24-25 0.2066486777301435 1103 26-27 0.22565804935160116 1103 28-29 0.1967334472066824 1103 30-31 0.10795180736504761 1103 32-33 0.23985195242977397 1103 34-35 0.22965882021689765 1103 36-37 0.18771765366295057 1103 38-39 0.17386061397763797 1103 40-41 0.23703943495624458 1103 42-43 0.2816617247914408 1103 44-45 0.27178157424316396 1103 46-47 0.10977273935800014 1103 48-49 0.09911790301313061 1103 50-51 0.12741301351557865 1103 52-53 0.13404205289912596 1103 54-55 0.14292973072176096 1103 56-57 0.34516113845185004 1103 58-59 0.326346968423465 1103 60-61 0.2786905932340247 1103 62-63 0.31607372561835945 1103 64-65 0.27534812178704016 1103 66-67 0.3536911274616159 1103 68-69 0.4385407168185793 1103 70-71 0.2908708800307167 1103 72-73 0.18581977718496745 1103 74-75 0.20126163058341717 1103 76-77 0.18088919572023343 1103 78-79 0.23337104871142245 1103 80-81 0.3725384884773959 1103 82-83 0.43974413491269715 1103 84-85 0.5080917092857256 1103 86-87 0.5467320391239916 1103 88-89 0.46652906360342783 1103 90-91 0.2897646274780996 1103 92-93 0.4123018320344016 1103 94-95 0.33408284556097456 1103 96-97 0.2703542371098102 1103 98-99 0.47627615170469184 1103 100-101 0.5972329167510857 1104 1 0.11448786258061006 1104 2 0.129964088858614 1104 3 0.03908856728849486 1104 4 0.06416046943621012 1104 5 0.05690359798447986 1104 6 -0.005542564796222393 1104 7 0.024248493462557974 1104 8 0.06271900805921149 1104 9 0.0503651212428764 1104 10-11 0.10353605780046138 1104 12-13 0.11172780769019397 1104 14-15 0.12704576316627936 1104 16-17 0.1626612930790543 1104 18-19 0.131395700120855 1104 20-21 0.17347860517784852 1104 22-23 0.1665196287220425 1104 24-25 0.0750184838836887 1104 26-27 0.10157536468241801 1104 28-29 0.1084828860934195 1104 30-31 0.10598099661087446 1104 32-33 0.02149582839178521 1104 34-35 0.13829672257092085 1104 36-37 0.07997529797264491 1104 38-39 0.16487565844359153 1104 40-41 0.17799277253158152 1104 42-43 0.2849994649748453 1104 44-45 0.28441912346306 1104 46-47 0.35135201082472634 1104 48-49 0.2427762699290099 1104 50-51 0.2883236672862495 1104 52-53 0.38808926663794807 1104 54-55 0.31220845428111943 1104 56-57 0.3476334436154147 1104 58-59 0.3286813534664006 1104 60-61 0.27991410771710434 1104 62-63 0.3372808215627785 1104 64-65 0.27734256029096827 1104 66-67 0.24036236582311687 1104 68-69 0.2517463211010238 1104 70-71 0.16163423218198858 1104 72-73 0.1847301532837946 1104 74-75 0.17515534820286405 1104 76-77 0.13425168093457174 1104 78-79 0.1883955354855651 1104 80-81 0.21363491130934875 1104 82-83 0.20917521166771635 1104 84-85 0.3645463526147701 1104 86-87 0.131064972800349 1104 88-89 0.05686985634557118 1104 90-91 0.09045008864598358 1104 92-93 0.30242455346762753 1104 94-95 0.2059024863385872 1104 96-97 0.39620955249264966 1104 98-99 0.35103186091999206 1104 100-101 0.24590421299141596 1105 1 0.08586970937189875 1105 2 0.0684476682406796 1105 3 0.07889532426655421 1105 4 -0.0036494620686795542 1105 5 0.01880584042350364 1105 6 0.054663046512054336 1105 7 0.04619052055598161 1105 8 0.001207969936260156 1105 9 0.004580359200694772 1105 10-11 -0.01258365575918674 1105 12-13 0.040148011056878374 1105 14-15 0.13221461890510255 1105 16-17 0.0710338212827537 1105 18-19 0.04022671382788445 1105 20-21 0.10215630939486431 1105 22-23 0.07342611417040956 1105 24-25 0.07723744606994387 1105 26-27 0.055165488834816756 1105 28-29 -0.05360636941590968 1105 30-31 0.1698900507690766 1105 32-33 0.07070271203026124 1105 34-35 0.12696800215030635 1105 36-37 0.11543156749134198 1105 38-39 0.11150129217512017 1105 40-41 0.09313430351929242 1105 42-43 0.08567050176934998 1105 44-45 0.13881541939034037 1105 46-47 0.010290731691974031 1105 48-49 0.14022147441227872 1105 50-51 0.1369088727544039 1105 52-53 0.11053839347128758 1105 54-55 0.2605100480722413 1105 56-57 0.25822951485216095 1105 58-59 0.20980328301068596 1105 60-61 0.1746005544802216 1105 62-63 0.29164052797630546 1105 64-65 0.3371100136035494 1105 66-67 0.3632963752894369 1105 68-69 0.3252323798664136 1105 70-71 0.37969147215308396 1105 72-73 0.3986291868396492 1105 74-75 0.30494883217734525 1105 76-77 0.2508855945466735 1105 78-79 0.3544391497278738 1105 80-81 0.4448971865472373 1105 82-83 0.4700357122855614 1105 84-85 0.507961843435325 1105 86-87 0.5941467260278408 1105 88-89 0.37159212533060426 1105 90-91 0.2964238564268271 1105 92-93 0.23316180640251716 1105 94-95 0.17845554113353046 1105 96-97 0.4720616758869163 1105 98-99 0.4544795779977342 1105 100-101 0.5202044027529915 1106 1 -0.10261341966032234 1106 2 0.029785304816627445 1106 3 0.05377615627580212 1106 4 4.0285606988987865E-4 1106 5 -0.004346325219728442 1106 6 0.048697975992610054 1106 7 0.0578615408192249 1106 8 -0.13110957320183303 1106 9 -0.06905305436819731 1106 10-11 0.005251627228261668 1106 12-13 -0.03926462279002152 1106 14-15 -0.1767950104639766 1106 16-17 -0.12619998173914837 1106 18-19 -0.062257151768918106 1106 20-21 0.029391446613097116 1106 22-23 0.04746006229994748 1106 24-25 0.04699331019362063 1106 26-27 -0.009702971535695326 1106 28-29 3.458839975891692E-4 1106 30-31 0.05637491756446167 1106 32-33 0.0892408739046644 1106 34-35 0.03480287716036656 1106 36-37 -0.034003218425276316 1106 38-39 -0.06693958363104713 1106 40-41 -0.04936448081834044 1106 42-43 -0.0866919519610505 1106 44-45 -0.03339305044082863 1106 46-47 -0.059910434241253085 1106 48-49 0.05945667117315878 1106 50-51 0.08549782230083736 1106 52-53 -0.16528784344448866 1106 54-55 -0.40222187880497984 1106 56-57 -0.19997783248986423 1106 58-59 -0.052861548125264335 1106 60-61 -0.10161700993886313 1106 62-63 0.00837350015775229 1106 64-65 -0.24053469983643794 1106 66-67 -0.3117146786925886 1106 68-69 -0.3240700339263114 1106 70-71 -0.2756270644650911 1106 72-73 -0.15240939128196374 1106 74-75 -0.23876969429652917 1106 76-77 -0.12670580617202987 1106 78-79 -0.14113027093320696 1106 80-81 -0.3410192685041302 1106 82-83 -0.3759489080694216 1106 84-85 -0.2835877870143442 1106 86-87 -0.13586359469752196 1106 88-89 -0.04144517813731241 1106 90-91 -0.31104290168558535 1106 92-93 -0.1617714451883181 1106 94-95 -0.14501087736526586 1106 96-97 -0.01727462208212316 1106 98-99 -0.03716730544092073 1106 100-101 -0.01822772910529835 1107 1 0.06403952307306326 1107 2 0.09455999424261563 1107 3 0.09781853382120431 1107 4 0.05030045172080122 1107 5 0.06093439923590438 1107 6 0.04275232696959819 1107 7 0.05607634418186791 1107 8 0.01144914912798356 1107 9 -0.0029746262907082155 1107 10-11 0.031209186664803212 1107 12-13 0.0325488689567095 1107 14-15 0.11708782635705717 1107 16-17 0.08582102848237838 1107 18-19 0.07576647580274454 1107 20-21 0.109109923928699 1107 22-23 0.08061417926986536 1107 24-25 0.04028204424480464 1107 26-27 0.07354094617112139 1107 28-29 0.09446545866769185 1107 30-31 0.08980941239067164 1107 32-33 0.08012489126404176 1107 34-35 0.19463379970276407 1107 36-37 0.14127001738410883 1107 38-39 0.0676348554031776 1107 40-41 0.08365903122020768 1107 42-43 0.02604909212218587 1107 44-45 -0.09447761227625051 1107 46-47 -0.06290725813098419 1107 48-49 -0.0761448572248753 1107 50-51 -0.11374769396412177 1107 52-53 0.02629073797695014 1107 54-55 -0.05106108612179128 1107 56-57 -0.03890240827328029 1107 58-59 -0.04429697351817907 1107 60-61 0.03213976975420252 1107 62-63 0.18035010365231585 1107 64-65 0.2307251782446187 1107 66-67 0.022342172000819005 1107 68-69 0.13269800079245186 1107 70-71 0.1704390855935216 1107 72-73 0.06762861911182227 1107 74-75 0.0756146507567621 1107 76-77 0.030428468511093598 1107 78-79 0.16091597802174462 1107 80-81 0.13641175822813167 1107 82-83 0.13424993491897652 1107 84-85 0.2027555689914884 1107 86-87 0.2159648218266561 1107 88-89 0.3427725786717879 1107 90-91 0.26766844198551354 1107 92-93 0.45098004939002934 1107 94-95 0.3383555579550972 1107 96-97 0.5158865844196576 1107 98-99 0.6193204039365874 1107 100-101 0.6451092557745675 1108 1 0.07620665706226504 1108 2 0.064411612583271 1108 3 0.07633641903814947 1108 4 0.06260089419150461 1108 5 0.013433224176338854 1108 6 0.06843872383178962 1108 7 0.11797726640102724 1108 8 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-0.40773123301973513 2316 88-89 -0.4472930078166151 2316 90-91 -0.4492556655862927 2316 92-93 -0.5750413809861605 2316 94-95 -0.5254674706246725 2316 96-97 -0.5331426519230149 2316 98-99 -0.3815842127401048 2316 100-101 -0.3168698423133556 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 12.0 4 24.0 5 37.0 6 87.0 7 274.0 8 613.0 9 1172.0 10 2099.0 11 3401.0 12 5347.0 13 7668.0 14 10617.0 15 14767.0 16 18862.0 17 27002.0 18 26265.0 19 18991.0 20 15928.0 21 15050.0 22 15287.0 23 16182.0 24 18983.0 25 21788.0 26 25473.0 27 31061.0 28 37539.0 29 46520.0 30 57011.0 31 70629.0 32 88579.0 33 116769.0 34 164934.0 35 254000.0 36 433328.0 37 710795.0 38 634084.0 39 78724.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.90390486408965 18.839885588605952 15.263793717005719 22.992415830298686 2 15.630939677463596 19.87041007817544 39.95825291109008 24.540397333270892 3 19.260922747793174 23.53412598784962 30.277944182378103 26.9270070819791 4 13.088244737637764 16.91812334276059 36.70225076858449 33.29138115101715 5 13.56054195611239 37.2005177413367 34.47100414393744 14.76793615861347 6 32.35790886153171 36.44578837102898 16.79069484319271 14.405607924246599 7 28.455419423715476 33.08341516405834 20.146311998480222 18.314853413745958 8 26.697071240820346 34.51515618744567 19.369136302506337 19.418636269227644 9 26.004483099077692 16.888513847973307 20.250169236655722 36.856833816293275 10-11 25.04112161160596 26.433520451438035 26.920240461926635 21.605117475029363 12-13 26.151221173064627 23.71143364754548 27.401943602353512 22.73540157703638 14-15 22.527368698476742 25.20803287855964 26.014896090587282 26.24970233237634 16-17 21.951338204396 28.332717928547492 27.106373386151116 22.609570480905393 18-19 22.055883250075585 26.469474686880417 29.685271673547582 21.789370389496412 20-21 22.34159400009097 25.623596948672738 30.168128694088654 21.866680357147636 22-23 22.17867950628647 25.561471374408345 30.174667348069107 22.085181771236076 24-25 22.12847747862856 25.843418386494065 30.072952880820996 21.955151254056375 26-27 21.524935652841812 26.242383551213784 29.83573828118989 22.396942514754517 28-29 21.78330361952185 26.79400376900788 29.565172748424157 21.85751986304611 30-31 21.9069491008431 26.147023725112977 29.477288963688565 22.468738210355355 32-33 21.44634317078938 26.190834539895828 29.538154967776503 22.824667321538286 34-35 22.41855923342704 26.380634692785566 29.197025595753125 22.003780478034273 36-37 22.57518950710843 25.734285171009187 29.352559397457117 22.337965924425266 38-39 21.91643141509216 26.235844344913435 29.173837950887698 22.673886289106708 40-41 22.497569774077636 26.101579763928555 29.208689671690358 22.19216079030345 42-43 22.149809459364988 26.10906758012892 29.01226598020973 22.728856980296364 44-45 22.187236920397066 26.3656392159145 29.267278675321347 22.179845188367086 46-47 22.771342897607504 26.760407456876234 28.32906592720219 22.139183718314072 48-49 22.33537308121443 26.84194384316328 28.15731700464763 22.665366070974663 50-51 22.464674545605355 27.093255587455417 28.231650296062227 22.210419570877 52-53 22.980542571152597 26.615917123982246 28.243473386507695 22.16006691835746 54-55 22.24416638349222 26.965007665777453 28.376671286646566 22.41415466408376 56-57 22.481163325208232 27.014323498484238 28.140577381541664 22.363935794765865 58-59 22.50116391385432 26.565865483666407 28.004135238762878 22.92883536371639 60-61 21.952987245109554 26.975944442512024 28.454717061906837 22.616351250471585 62-63 22.10638640869698 26.98367042240708 28.560940924013817 22.349002244882126 64-65 21.736291904243334 27.070947238580935 28.330332130176405 22.862428726999322 66-67 21.69306320721145 27.08591423426509 28.261350166827654 22.95967239169581 68-69 22.466390225238 26.624935114973592 28.41079512920816 22.497879530580246 70-71 22.149063250022074 26.932247504274375 27.87906466812401 23.03962457757954 72-73 22.155886193306024 27.109827981559327 27.95328089923717 22.781004925897484 74-75 22.206690364131116 27.519940386205345 27.645044402644626 22.62832484701891 76-77 22.259936078386254 26.923434535865937 28.017429944426457 22.79919944132135 78-79 22.33418575529982 27.33023580627122 27.781035027117856 22.5545434113111 80-81 22.652807172920138 27.415990838526348 27.398983649017094 22.532218339536414 82-83 22.453644941270827 27.49091862600592 27.598413464481048 22.4570229682422 84-85 22.370540528246554 27.74618906313967 27.552197192068007 22.331073216545768 86-87 22.653168797392826 27.71149174020303 27.089732646934483 22.54560681546966 88-89 22.352486313128594 27.8705137848677 26.849295413338663 22.92770448866504 90-91 22.251346155036057 27.891175119472877 27.08944382671754 22.76803489877352 92-93 22.532485189308943 27.385990372256476 27.416192041141162 22.665332397293422 94-95 22.144280500563227 27.233109201876175 27.5990898728792 23.023520424681397 96-97 22.521201714498908 26.7543704888924 27.663962628994206 23.060465167614495 98-99 22.19438230957968 27.293997281525346 27.08059635199966 23.431024056895318 100-101 22.186606160984002 26.969740246535682 27.51744867407469 23.326204918405622 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 727.0 1 511.5 2 454.5 3 629.0 4 512.5 5 478.5 6 981.0 7 2350.5 8 3948.0 9 4498.5 10 3728.0 11 2784.5 12 2688.0 13 2933.0 14 3074.0 15 3271.0 16 3532.0 17 3818.0 18 4216.5 19 4760.0 20 5410.0 21 6430.0 22 7943.5 23 9789.0 24 12315.0 25 15762.0 26 20218.5 27 25619.0 28 30058.5 29 33633.5 30 37960.0 31 44761.5 32 55337.5 33 58968.0 34 56933.0 35 59032.5 36 63568.0 37 68501.0 38 73198.0 39 76595.5 40 80266.5 41 87046.0 42 91323.0 43 93283.5 44 96512.0 45 103028.0 46 106731.5 47 109638.0 48 112256.0 49 108044.0 50 104675.5 51 102585.0 52 100258.0 53 102823.5 54 107249.0 55 101831.0 56 91947.0 57 84461.5 58 79412.5 59 69966.5 60 54457.0 61 45291.0 62 39865.5 63 29277.0 64 19364.0 65 14730.5 66 11039.5 67 8424.5 68 6485.0 69 4761.5 70 3506.0 71 2351.0 72 1525.0 73 1189.5 74 900.0 75 658.5 76 473.5 77 288.0 78 187.5 79 109.0 80 57.0 81 25.0 82 12.5 83 10.5 84 8.5 85 5.0 86 3.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.016120875798351256 3 0.007057063886830114 4 0.0 5 6.689160082303426E-5 6 0.0 7 0.0 8 3.679038045266884E-4 9 5.351328065842741E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.344580041151713E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005819569271603981 26-27 0.02200733667077827 28-29 1.6722900205758566E-5 30-31 0.0 32-33 4.347954053497227E-4 34-35 0.020318323749996658 36-37 0.0038964357479417456 38-39 0.018696202430038073 40-41 0.015987092596705187 42-43 0.00872935390740597 44-45 0.002842893034978956 46-47 1.6722900205758566E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.6756640329213705E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.344580041151713E-5 84-85 0.003210796839505644 86-87 2.6756640329213705E-4 88-89 1.0033740123455137E-4 90-91 2.5084350308637843E-4 92-93 3.679038045266884E-4 94-95 0.0 96-97 1.6722900205758566E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2989912.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.14277484768719 #Duplication Level Percentage of deduplicated Percentage of total 1 70.51679300840938 33.24357295776405 2 15.526426528045073 14.639176600015727 3 5.7755495514147075 8.168262963720128 4 2.7217642867643033 5.132460838376217 5 1.491357798853235 3.515337246434021 6 0.9506492772981167 2.6889746903268996 7 0.5953007479340982 1.9644890388561882 8 0.42293995260613576 1.5950850367842038 9 0.3173198158490047 1.346340296995127 >10 1.5208710274472226 12.737504269230207 >50 0.10013774604284875 3.227576233664222 >100 0.05167030098240874 4.670629495497315 >500 0.004539056420288047 1.4295115199995954 >1k 0.004326288150364017 4.228619119670552 >5k 2.1276826969003366E-4 0.5984188325086267 >10k+ 1.418455131266891E-4 0.8140408601568629 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13339 0.446133531689227 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11008 0.36817137092998053 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7073 0.23656214631066064 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5506 0.1841525770658133 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5319 0.1778982123888596 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4972 0.16629251964606317 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4963 0.1659915074423595 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4552 0.15224528347322597 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4020 0.13445211765429885 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3710 0.12408391952672855 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3572 0.11946839906993918 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3494 0.11685962663784086 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3148 0.10528737969545592 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3038 0.10160834165018903 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.689160082303426E-5 2 0.0 0.0 0.0 0.0 6.689160082303426E-5 3 0.0 0.0 0.0 0.0 6.689160082303426E-5 4 0.0 0.0 0.0 0.0 6.689160082303426E-5 5 0.0 0.0 0.0 0.0 6.689160082303426E-5 6 0.0 0.0 0.0 0.0 1.3378320164606853E-4 7 0.0 0.0 0.0 0.0 1.3378320164606853E-4 8 0.0 0.0 0.0 0.0 1.3378320164606853E-4 9 0.0 0.0 0.0 0.0 1.3378320164606853E-4 10-11 0.0 0.0 0.0 3.344580041151713E-5 1.505061018518271E-4 12-13 0.0 0.0 0.0 3.344580041151713E-5 6.354702078188255E-4 14-15 0.0 0.0 0.0 3.344580041151713E-5 8.026992098764111E-4 16-17 0.0 0.0 0.0 6.689160082303426E-5 8.528679104936867E-4 18-19 0.0 0.0 0.0 1.003374012345514E-4 9.030366111109625E-4 20-21 0.0 0.0 0.0 2.1739770267486134E-4 9.364824115224796E-4 22-23 0.0 0.0 0.0 3.679038045266884E-4 9.866511121397552E-4 24-25 0.0 0.0 0.0 5.351328065842741E-4 0.0010200969125512725 26-27 0.0 0.0 0.0 0.0011204343137858237 0.0011037114135800654 28-29 0.0 0.0 0.0 0.0030602907376538176 0.0011371572139915825 30-31 0.0 0.0 0.0 0.01117089733744672 0.001153880114197341 32-33 0.0 0.0 0.0 0.0270409296327116 0.0012207717150203752 34-35 0.0 0.0 0.0 0.051422918132707586 0.001270940415637651 36-37 0.0 0.0 0.0 0.09202611983228937 0.001270940415637651 38-39 0.0 0.0 0.0 0.1522285605730202 0.0013712778168722022 40-41 0.0 0.0 0.0 0.21594281035696034 0.0013712778168722022 42-43 0.0 0.0 0.0 0.28163036236518 0.0013712778168722022 44-45 0.0 0.0 0.0 0.3638735855771006 0.001421446517489478 46-47 0.0 0.0 0.0 0.45966235795568566 0.001505061018518271 48-49 0.0 0.0 0.0 0.5574913241593733 0.0016053984197528222 50-51 0.0 0.0 0.0 0.6654878136881621 0.0016053984197528222 52-53 0.0 0.0 0.0 0.7668787576356763 0.001772627421810408 54-55 0.0 0.0 0.0 0.878136212704588 0.001839519022633442 56-57 0.0 0.0 0.0 1.0036917474494231 0.0018562419228392007 58-59 0.0 0.0 0.0 1.13523408046792 0.0018729648230449592 60-61 0.0 0.0 0.0 1.277445623817691 0.0018729648230449592 62-63 0.0 0.0 0.0 1.4157440085193143 0.0018729648230449592 64-65 0.0 0.0 0.0 1.5582565640727888 0.0018729648230449592 66-67 0.0 0.0 0.0 1.7247163127209095 0.0018729648230449592 68-69 0.0 0.0 0.0 1.9041363090284933 0.0018729648230449592 70-71 0.0 0.0 0.0 2.0928542378504784 0.0018896877232507177 72-73 0.0 0.0 0.0 2.287809808449212 0.002040193825102545 74-75 0.0 0.0 0.0 2.484153379765023 0.0021405312263370964 76-77 0.0 0.0 0.0 2.6934237529398857 0.0021405312263370964 78-79 0.0 0.0 0.0 2.9325110571816158 0.0021405312263370964 80-81 0.0 0.0 0.0 3.1755449658719055 0.002157254126542855 82-83 0.0 0.0 0.0 3.4315725680220686 0.0022408686275716475 84-85 0.0 0.0 0.0 3.694322776054947 0.0022408686275716475 86-87 0.0 0.0 0.0 3.964163493775068 0.002257591527777406 88-89 0.0 0.0 0.0 4.248837423977696 0.002291037328188923 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7785 0.0 46.619324 1 GTATCAA 12765 0.0 36.87942 1 TATCAAC 15785 0.0 29.402292 2 TCAACGC 15890 0.0 29.144316 4 ATCAACG 15955 0.0 29.056808 3 CAACGCA 16090 0.0 28.811567 5 AGGTAAG 2370 0.0 27.861061 2 AACGCAG 16690 0.0 27.83272 6 TAAGGTG 2575 0.0 27.115225 5 GTAAGGT 2530 0.0 27.034298 4 AAGGTAA 2420 0.0 26.107634 1 GTGTAAG 1270 0.0 25.809322 1 ACGCAGA 18600 0.0 24.974628 7 GGTAAGG 2675 0.0 24.859886 3 CGCAGAG 18685 0.0 24.835594 8 GAGTACT 12905 0.0 22.5803 12-13 GCAGAGT 20540 0.0 22.430782 9 GTACTTT 13560 0.0 21.279417 14-15 AGAGTAC 17350 0.0 21.10672 10-11 GGTGTGC 3665 0.0 20.865316 8 >>END_MODULE