FastQCFastQC Report
Thu 26 May 2016
SRR938236_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938236_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2993174
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130840.4371279451177913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107200.3581482399619935No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66660.2227067320509934No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA54660.18261551115972544No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC48660.16256990071409147No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA48630.1624696726618633No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA47230.15779236355788204No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA43460.14519703832787537No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG39090.13059715205330527No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA38500.12862600035948463No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA36950.12344755099436251No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA34530.11536248811462348No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT32130.10734424393636988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71000.046.618661
GTATCAA123650.040.249041
TCAACGC155300.031.6960644
TATCAAC156700.031.5646362
ATCAACG157400.031.2731843
CAACGCA158550.030.9563645
AACGCAG163850.029.9770246
GTGTAAG13550.027.0635491
ACGCAGA182700.026.6189987
CGCAGAG183600.026.3827348
TAAGGTG21150.024.2856565
GCAGAGT197800.024.080189
GTACATG94050.023.6984751
AGGTAAG21500.022.7842772
TACATGG92550.022.662022
GAGTACT129700.022.53978512-13
AGAGTAC169600.022.14158210-11
GTACTTT133650.021.7830714-15
ACATGGG93350.021.6017043
GGTAAGG23700.021.471973
Produced by FastQC (version 0.11.5)