##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938236_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2993174 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.016236944460964 33.0 31.0 34.0 30.0 34.0 2 32.136524304968574 34.0 31.0 34.0 30.0 34.0 3 32.16285087335384 34.0 31.0 34.0 30.0 34.0 4 35.67737358402819 37.0 35.0 37.0 35.0 37.0 5 35.683397289967104 37.0 35.0 37.0 35.0 37.0 6 35.71567406371965 37.0 36.0 37.0 35.0 37.0 7 35.65507551515548 37.0 35.0 37.0 33.0 37.0 8 35.643767452209595 37.0 35.0 37.0 33.0 37.0 9 37.29095034234562 39.0 37.0 39.0 34.0 39.0 10-11 37.19955321675252 39.0 37.0 39.0 34.0 39.0 12-13 37.054198152195625 39.0 37.0 39.0 33.0 39.0 14-15 38.24195669880869 40.0 38.0 41.0 33.5 41.0 16-17 38.06813302534367 40.0 38.0 41.0 33.0 41.0 18-19 38.25248314999395 40.0 38.0 41.0 33.5 41.0 20-21 38.251419730359814 40.0 38.0 41.0 34.0 41.0 22-23 38.14293255253453 40.0 38.0 41.0 33.5 41.0 24-25 38.01954179743643 40.0 38.0 41.0 33.0 41.0 26-27 37.83635849436084 40.0 38.0 41.0 33.0 41.0 28-29 37.65706153401038 40.0 38.0 41.0 32.0 41.0 30-31 37.41483505469445 40.0 37.0 41.0 32.0 41.0 32-33 37.10565556830308 40.0 36.5 41.0 31.0 41.0 34-35 36.85546229520904 39.5 36.0 41.0 30.5 41.0 36-37 36.568840802439155 39.0 35.5 41.0 30.0 41.0 38-39 36.24979052337085 39.0 35.0 40.5 30.0 41.0 40-41 36.11229517562293 39.0 35.0 41.0 29.5 41.0 42-43 35.876970734076934 39.0 35.0 40.5 28.5 41.0 44-45 35.53198343965302 39.0 35.0 40.0 27.0 41.0 46-47 35.13541945773951 38.0 35.0 40.0 25.5 41.0 48-49 34.701121618723135 38.0 34.0 40.0 24.0 41.0 50-51 33.944548997151514 37.5 33.5 39.5 23.0 40.5 52-53 34.00008151881582 38.0 33.5 39.5 23.0 40.5 54-55 34.063092389550356 38.0 34.0 40.0 22.5 41.0 56-57 33.73012811817823 38.0 33.0 40.0 20.5 41.0 58-59 33.29978460991576 37.0 33.0 40.0 18.5 41.0 60-61 32.77314482886729 37.0 32.0 40.0 14.0 41.0 62-63 32.22347965738042 36.0 31.5 39.0 8.5 41.0 64-65 31.670642100993796 35.5 31.0 39.0 7.0 40.0 66-67 31.077170588813082 35.0 30.5 38.5 2.0 40.0 68-69 30.385050952600817 35.0 29.0 38.0 2.0 40.0 70-71 29.778552132284993 34.5 28.5 37.0 2.0 39.5 72-73 29.081271920710257 34.0 27.5 36.5 2.0 39.0 74-75 28.400638085189833 34.0 26.0 36.0 2.0 39.0 76-77 27.650113892476682 33.5 25.5 35.5 2.0 38.0 78-79 26.87693348265086 33.0 24.5 35.0 2.0 37.0 80-81 26.14788181375356 32.0 22.0 35.0 2.0 36.5 82-83 25.392905156866924 31.5 19.5 35.0 2.0 36.0 84-85 24.65734701691248 31.0 16.5 34.0 2.0 35.5 86-87 23.90122809432395 31.0 7.5 34.0 2.0 35.0 88-89 22.998907180137206 30.0 2.0 34.0 2.0 35.0 90-91 22.08488915111517 29.0 2.0 34.0 2.0 35.0 92-93 21.127151812757962 29.0 2.0 34.0 2.0 35.0 94-95 20.01932263209556 27.0 2.0 34.0 2.0 35.0 96-97 18.724989091846982 25.5 2.0 33.0 2.0 35.0 98-99 17.125447601776575 20.0 2.0 32.5 2.0 34.0 100-101 13.214225267224691 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1685207959311299 1101 2 0.2488755813425101 1101 3 0.2193850396649637 1101 4 0.0521848790228745 1101 5 0.14846803085868743 1101 6 0.19068962029680847 1101 7 0.2257376947818699 1101 8 0.2203272876313136 1101 9 0.20892062657586763 1101 10-11 0.2783474481167332 1101 12-13 0.26628389628971405 1101 14-15 0.37361204793963765 1101 16-17 0.33962972506044764 1101 18-19 0.43086704728370506 1101 20-21 0.3838279082045588 1101 22-23 0.33236619066698836 1101 24-25 0.37052382817214635 1101 26-27 0.3547728261945906 1101 28-29 0.277900698466901 1101 30-31 0.34218604687941223 1101 32-33 0.21504760992295502 1101 34-35 0.2752140243862371 1101 36-37 0.29865041354459976 1101 38-39 0.31117945519982015 1101 40-41 0.4443705368172459 1101 42-43 0.4666789361200827 1101 44-45 0.358916564725142 1101 46-47 0.39455883192007946 1101 48-49 0.5207664251945587 1101 50-51 0.374049461855698 1101 52-53 0.3434733397099805 1101 54-55 0.2808909530078765 1101 56-57 0.304891278876255 1101 58-59 0.28093067238240366 1101 60-61 0.21434978184369413 1101 62-63 0.20389720881491513 1101 64-65 0.072801273378726 1101 66-67 0.20537358339699452 1101 68-69 0.2585400655442953 1101 70-71 0.08667110324603655 1101 72-73 0.16847959447378003 1101 74-75 0.057171945285755754 1101 76-77 -0.07901600257860508 1101 78-79 -0.09913077543646764 1101 80-81 -0.03389776767845376 1101 82-83 -0.10779284422238788 1101 84-85 -0.03026413551542717 1101 86-87 0.16251133142905871 1101 88-89 0.2859146515036741 1101 90-91 0.1339470899083537 1101 92-93 -0.03621688608271256 1101 94-95 -0.4058678223143346 1101 96-97 -0.543287691417607 1101 98-99 -0.8260113011403085 1101 100-101 -0.8356627427492604 1102 1 0.217407968060769 1102 2 0.21946013786680396 1102 3 0.1979010239198331 1102 4 0.1592186869175123 1102 5 0.09481685181296484 1102 6 0.20646418528227883 1102 7 0.18122457996746277 1102 8 0.19948521721966017 1102 9 0.2588967145720389 1102 10-11 0.2715693147245446 1102 12-13 0.3084922618122832 1102 14-15 0.3404369017085429 1102 16-17 0.25139075217298057 1102 18-19 0.2128916582537883 1102 20-21 0.33704407271913794 1102 22-23 0.39859508372515506 1102 24-25 0.2694036427621427 1102 26-27 0.3003518645641563 1102 28-29 0.14655574022092566 1102 30-31 0.1720870879712706 1102 32-33 0.2408596983605733 1102 34-35 0.1873374103259806 1102 36-37 0.09139955823061285 1102 38-39 -0.09215902841126677 1102 40-41 0.06359394850836964 1102 42-43 0.07419834163088268 1102 44-45 0.06018390092791748 1102 46-47 0.21443706463905698 1102 48-49 0.09321795740218874 1102 50-51 -0.01780850084158203 1102 52-53 -0.27288247090694995 1102 54-55 -0.15010268272033755 1102 56-57 -0.13074956282627426 1102 58-59 -0.020452545069687744 1102 60-61 -0.012716767102340043 1102 62-63 -0.030152564940742366 1102 64-65 -0.0853352457692722 1102 66-67 -0.12911028519734913 1102 68-69 -0.28899331929181216 1102 70-71 -0.523177883252874 1102 72-73 -0.5373650510767511 1102 74-75 -0.48404223269313107 1102 76-77 -0.69284357863717 1102 78-79 -0.7550517554607445 1102 80-81 -0.6901415597543838 1102 82-83 -0.7549298416104655 1102 84-85 -0.6680331455584678 1102 86-87 -0.5372421909930445 1102 88-89 -0.5875908482572072 1102 90-91 -0.4906142167906893 1102 92-93 -0.48667213384786123 1102 94-95 -0.7174237580094385 1102 96-97 -0.9259192994078944 1102 98-99 -0.9893293675788932 1102 100-101 -1.291763062802234 1103 1 0.28070293281915326 1103 2 0.23769788781187629 1103 3 0.2832699200204303 1103 4 0.1324384357470194 1103 5 0.21733599384430136 1103 6 0.20071937856186395 1103 7 0.2938409111203768 1103 8 0.23321913875845723 1103 9 0.17163474340762974 1103 10-11 0.2563283702153072 1103 12-13 0.2539537833713652 1103 14-15 0.3051117829687868 1103 16-17 0.2329549054444584 1103 18-19 0.17555886579905433 1103 20-21 0.18234468289779926 1103 22-23 0.21651033481113302 1103 24-25 0.13956911780567083 1103 26-27 0.1503814438620381 1103 28-29 0.13876776462807783 1103 30-31 0.07044416925517538 1103 32-33 0.05340030651094452 1103 34-35 -0.02595062442546947 1103 36-37 -0.05211311016010001 1103 38-39 -0.061511833373820934 1103 40-41 0.059140827469889246 1103 42-43 0.1919269896034379 1103 44-45 0.16785423542751943 1103 46-47 0.14511975483394224 1103 48-49 0.1453332997614396 1103 50-51 0.12488575063316176 1103 52-53 0.20954810225533294 1103 54-55 0.15053589206458184 1103 56-57 0.065864537202458 1103 58-59 0.1310370683711497 1103 60-61 -0.044743202543408245 1103 62-63 -0.0669370417252182 1103 64-65 -0.1616924284679193 1103 66-67 -0.3516088062324556 1103 68-69 -0.1686035486581403 1103 70-71 -0.39058318724354635 1103 72-73 -0.40369907741077427 1103 74-75 -0.0997317683826644 1103 76-77 -0.3473905083060522 1103 78-79 -0.3950176124997711 1103 80-81 -0.6415882409571658 1103 82-83 -0.6847096026097361 1103 84-85 -0.9228211042488645 1103 86-87 -1.0179101760512737 1103 88-89 -0.8002179969819174 1103 90-91 -0.7352995775736098 1103 92-93 -0.777491395886635 1103 94-95 -0.9203509253268507 1103 96-97 -0.9493646960240234 1103 98-99 -1.2536993285342177 1103 100-101 -1.0938860112666458 1104 1 0.2824949340253795 1104 2 0.275865553385394 1104 3 0.2646039318565343 1104 4 0.25682872561848313 1104 5 0.2253705974856004 1104 6 0.23541310995015863 1104 7 0.2193937024053909 1104 8 0.21516342614207673 1104 9 0.3235605604380538 1104 10-11 0.27518886250675934 1104 12-13 0.25913132492966184 1104 14-15 0.33481682273659175 1104 16-17 0.37275700880304186 1104 18-19 0.38196261264212694 1104 20-21 0.37434268508408763 1104 22-23 0.5040886453392375 1104 24-25 0.4011583220727033 1104 26-27 0.34487897486584274 1104 28-29 0.29454559266532243 1104 30-31 0.30387833563913347 1104 32-33 0.4030631283285757 1104 34-35 0.4865434386752838 1104 36-37 0.43953169363951616 1104 38-39 0.26996164847623305 1104 40-41 0.3114922165158305 1104 42-43 0.2548848847370593 1104 44-45 0.2105829474030969 1104 46-47 0.19946726133625958 1104 48-49 0.0013210867115631686 1104 50-51 0.1464419721266168 1104 52-53 0.25838164290895094 1104 54-55 0.3608400874733775 1104 56-57 0.3825306384501488 1104 58-59 0.35118238104784183 1104 60-61 0.304305574266877 1104 62-63 0.37379819141587234 1104 64-65 0.17884660428869026 1104 66-67 0.2623472418658501 1104 68-69 0.2857469734120457 1104 70-71 0.21097454203384913 1104 72-73 0.3002677136480649 1104 74-75 0.048320174773881064 1104 76-77 -0.1545230802084987 1104 78-79 -0.18283566067346868 1104 80-81 -0.444118679345344 1104 82-83 -0.3348055353625732 1104 84-85 -0.4523044997782897 1104 86-87 -0.3983087964289709 1104 88-89 -0.43517766376166733 1104 90-91 -0.5827670659825195 1104 92-93 -0.8314523442827308 1104 94-95 -0.7134946548304129 1104 96-97 -0.8686030766640762 1104 98-99 -0.7801313463260691 1104 100-101 -1.0051614080022961 1105 1 0.1683234153002715 1105 2 0.23790121826673527 1105 3 0.23460436799662432 1105 4 0.17162425651000035 1105 5 0.2511372322002643 1105 6 0.2186238499945503 1105 7 0.20521369791124755 1105 8 0.2252482174632675 1105 9 0.23509381090477177 1105 10-11 0.18541013723045552 1105 12-13 0.17307768633619247 1105 14-15 0.2850168545937777 1105 16-17 0.2877291290222104 1105 18-19 0.19708366047816384 1105 20-21 0.2542878973563134 1105 22-23 0.293617040429055 1105 24-25 0.21625317780040376 1105 26-27 0.2721304532385034 1105 28-29 0.22840660809425373 1105 30-31 0.21554586510466578 1105 32-33 0.18323702970592137 1105 34-35 0.2475525388329629 1105 36-37 0.32779532989109583 1105 38-39 0.3095937453524229 1105 40-41 0.22502246709727558 1105 42-43 0.29752065941192285 1105 44-45 0.3504440652301355 1105 46-47 0.33325548403697525 1105 48-49 0.22497514418913767 1105 50-51 0.2982133882639886 1105 52-53 0.2819482590402558 1105 54-55 0.2981494539609386 1105 56-57 0.255970489517793 1105 58-59 0.2803810826777706 1105 60-61 0.3018391309797792 1105 62-63 0.4621368247993658 1105 64-65 0.2577929372513026 1105 66-67 0.3132447897156361 1105 68-69 0.3436162442142674 1105 70-71 0.187040051222656 1105 72-73 -0.014246293559622814 1105 74-75 0.003757798682702429 1105 76-77 -0.11485353217288363 1105 78-79 -0.12180058173685282 1105 80-81 -0.019948981031024715 1105 82-83 -0.1056574165011277 1105 84-85 -0.13711921609286648 1105 86-87 -0.2325498463129705 1105 88-89 -0.11968921531105536 1105 90-91 -0.3461346410531334 1105 92-93 -0.5862178858027889 1105 94-95 -0.7425567545415177 1105 96-97 -0.864663475428852 1105 98-99 -0.7259450889105032 1105 100-101 -0.8448959266885279 1106 1 0.15370332752592475 1106 2 0.1392441689123416 1106 3 0.16066527079011195 1106 4 0.1280952762924059 1106 5 0.15163185773278798 1106 6 0.09461084112358265 1106 7 0.11917388437034049 1106 8 0.01064260372996273 1106 9 0.2670323239243544 1106 10-11 0.2415469477474872 1106 12-13 0.23625478537260136 1106 14-15 0.18812124900669147 1106 16-17 -0.271532010372205 1106 18-19 0.24991809051184788 1106 20-21 0.30845529774016 1106 22-23 0.3513900184917418 1106 24-25 0.2904305480291356 1106 26-27 0.14384316076725412 1106 28-29 0.12252364195044407 1106 30-31 0.06419533779497044 1106 32-33 -0.007428023740907008 1106 34-35 0.06169657696500508 1106 36-37 0.33169234787287394 1106 38-39 0.31612691990674335 1106 40-41 0.2781073189421406 1106 42-43 -0.014650833415586817 1106 44-45 0.1648832013650221 1106 46-47 0.39722617332007104 1106 48-49 0.4808513697145571 1106 50-51 0.34771095092953175 1106 52-53 0.3625241220876134 1106 54-55 0.32628213023203756 1106 56-57 0.09496820659052219 1106 58-59 0.2795800463991398 1106 60-61 0.5940316137794497 1106 62-63 0.3853000454772797 1106 64-65 0.21428093921182878 1106 66-67 0.04277603979727829 1106 68-69 0.03382604465538819 1106 70-71 0.10650429224208935 1106 72-73 0.07647341637432348 1106 74-75 -0.07937126952869988 1106 76-77 -0.38146014289067764 1106 78-79 -0.32497862654994947 1106 80-81 -0.3205563943526819 1106 82-83 -0.17170803070731822 1106 84-85 -0.10381050256340174 1106 86-87 -0.2840635854175986 1106 88-89 -0.45957379397798803 1106 90-91 -0.411926081094812 1106 92-93 -0.5168483008315476 1106 94-95 -0.8935007546334859 1106 96-97 -0.7547770261096822 1106 98-99 -1.1161392396213223 1106 100-101 -1.2547525657534937 1107 1 0.1400374688665309 1107 2 0.09794818849038478 1107 3 0.021710930300237408 1107 4 0.11478009730782901 1107 5 0.11018268184211877 1107 6 0.03290888544910331 1107 7 0.09517229287997253 1107 8 0.15757012702632522 1107 9 0.01633701494869655 1107 10-11 0.09334473166491364 1107 12-13 0.013142405242611233 1107 14-15 0.0207912596747164 1107 16-17 0.11135041682484825 1107 18-19 0.023099372300514176 1107 20-21 0.0561115038749449 1107 22-23 0.2194883569138426 1107 24-25 0.12804915956027685 1107 26-27 0.19208158228760652 1107 28-29 0.2686975306661523 1107 30-31 0.05952783730609923 1107 32-33 0.04915313742750271 1107 34-35 0.027474330756412257 1107 36-37 0.08026905790216432 1107 38-39 0.042346893110853046 1107 40-41 0.05341494415584691 1107 42-43 0.10932447525028977 1107 44-45 0.1379996957493077 1107 46-47 0.22491285397843086 1107 48-49 0.17261845645465002 1107 50-51 0.11947257297186553 1107 52-53 0.10320412603729778 1107 54-55 0.009955752731293899 1107 56-57 0.12129047951756888 1107 58-59 0.15405100169994057 1107 60-61 0.19420221012367023 1107 62-63 0.17394547807418093 1107 64-65 0.07718938785722074 1107 66-67 0.05492204451753935 1107 68-69 -0.10524531006690552 1107 70-71 -0.09332588586155666 1107 72-73 -0.11487909874193036 1107 74-75 -0.19997180060632402 1107 76-77 -0.21243629086442795 1107 78-79 -0.31204764631192106 1107 80-81 -0.3849611730832443 1107 82-83 -0.38467550680108076 1107 84-85 -0.4542713958502915 1107 86-87 -0.6748458648887237 1107 88-89 -0.6836525398371158 1107 90-91 -0.6851984815153358 1107 92-93 -0.686859573888313 1107 94-95 -0.7241540546526508 1107 96-97 -0.7091648299110567 1107 98-99 -0.941084616845199 1107 100-101 -0.7159512755233468 1108 1 -0.029107014886854188 1108 2 0.03603542384508529 1108 3 0.09537471109830165 1108 4 0.15684850643135206 1108 5 0.14571352604149723 1108 6 0.16042265102656472 1108 7 0.13657825083956254 1108 8 0.09092813140796352 1108 9 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Count 2 9117.0 3 2639.0 4 2450.0 5 2796.0 6 3286.0 7 4084.0 8 5320.0 9 6736.0 10 8711.0 11 11127.0 12 14469.0 13 17920.0 14 21213.0 15 26375.0 16 31273.0 17 38919.0 18 38068.0 19 33440.0 20 33517.0 21 35312.0 22 38162.0 23 43226.0 24 47798.0 25 53298.0 26 60776.0 27 68906.0 28 79747.0 29 92505.0 30 108033.0 31 129033.0 32 157025.0 33 194809.0 34 249384.0 35 324350.0 36 397108.0 37 400498.0 38 190952.0 39 10792.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.61053803001822 18.41873338334363 14.839573355532373 23.13115523110578 2 15.426737936454188 19.545787139281938 40.388222234181484 24.639252690082387 3 19.221013092529038 23.531553199085472 30.021705445031888 27.2257282633536 4 12.827916069543296 16.674829025347467 36.928751975357706 33.56850292975153 5 13.608032429645945 37.08712287314345 34.3761946046549 14.92865009255571 6 32.230239428251025 36.26793201275388 16.935452285907964 14.566376273087126 7 28.355888920828832 33.12938990652379 20.263217866528436 18.251503306118938 8 26.699061261088975 34.26520728283263 19.5316402287637 19.504091227314703 9 26.172460900129916 16.85214769976759 20.285670896102523 36.689720503999965 10-11 25.014631637245095 26.348970066512916 26.95047249319085 21.68592580305114 12-13 26.232935083637788 23.75217530526212 27.33198914162563 22.682900469474458 14-15 22.70003867421059 25.161973348249894 26.03098888216811 26.106999095371407 16-17 22.00325979175337 28.28846213034617 27.03049497716406 22.6777831007364 18-19 22.126077591794456 26.417710951572577 29.627984845010275 21.828226611622696 20-21 22.399356348986643 25.65339024938968 30.087306041169914 21.85994736045377 22-23 22.213057572596746 25.55084416688648 30.094659969926262 22.14143829059051 24-25 22.13833513179071 25.795988976901512 30.023145024857552 22.042530866450228 26-27 21.52626855159734 26.16069867680677 29.800931312561847 22.512101459034042 28-29 21.793156532808762 26.76058034175354 29.545793244845537 21.90046988059216 30-31 21.894078736154956 26.155224998662312 29.3831598801434 22.56753638503933 32-33 21.48436258661667 26.194419402802843 29.44637251146944 22.874845499111046 34-35 22.47844625428897 26.354818219195696 29.108855465713564 22.057880060801768 36-37 22.619183960015736 25.67563478145434 29.267851867357347 22.43732939117257 38-39 21.960466497270957 26.26280892808883 29.053726675011106 22.722997899629114 40-41 22.565527592918684 26.11230388523071 29.128825455034736 22.193343066815874 42-43 22.21383685644059 26.140277419101416 28.889028180679283 22.756857543778715 44-45 22.213398574847258 26.360713378521105 29.126542467145285 22.299345579486356 46-47 22.806613198485707 26.740781385090738 28.241638695510986 22.210966720912577 48-49 22.410935644710204 26.843802407693524 28.029015322369723 22.716246625226553 50-51 22.554386476289835 27.08484208404568 28.08571938097501 22.275052058689475 52-53 22.974942632947613 26.6363081768247 28.11915437130005 22.269594818927644 54-55 22.24517137434409 26.977411705560634 28.27837124678855 22.499045673306732 56-57 22.449003462150277 27.0411815423278 28.08514015693299 22.42467483858894 58-59 22.56107976244317 26.533658508213442 27.93274950269436 22.97251222664903 60-61 22.007540379432054 26.894866669708083 28.39239785636782 22.70519509449204 62-63 22.15453839843883 26.896589992131315 28.558606982336578 22.390264627093273 64-65 21.83549846054709 26.997612277120243 28.288483314683425 22.878405947649245 66-67 21.770017214275363 26.967638800017447 28.263163004866154 22.999180980841036 68-69 22.566067470835733 26.47207052278456 28.419831683133562 22.542030323246145 70-71 22.223308618650282 26.755838085482015 27.90312285488936 23.117730440978338 72-73 22.214922228042163 26.849200319157006 28.009169580071873 22.926707872728958 74-75 22.2790749054388 27.322015792593568 27.727501303125447 22.671407998842184 76-77 22.393533319365122 26.785316268054665 27.91495363923718 22.906196773343037 78-79 22.506103193978394 27.08601748276227 27.71066441146597 22.69721491179337 80-81 22.821748776646352 27.155226142055728 27.24278160359499 22.780243477702932 82-83 22.618789433988493 27.224886474557568 27.474710952477544 22.681613138976395 84-85 22.568829381727678 27.431087078344746 27.45088604117984 22.549197498747738 86-87 22.896138450875643 27.288410807819474 27.05490461585349 22.760546125451388 88-89 22.547113877488457 27.422472278604516 26.87072720595647 23.15968663795056 90-91 22.50281070662243 27.374919583605706 27.05328745229993 23.06898225747193 92-93 22.791130869775213 26.83113012748785 27.311961810318497 23.065777192418437 94-95 22.46528405836738 26.658446222345855 27.49902454665241 23.377245172634346 96-97 22.848213209626568 26.166993100835978 27.533035266089957 23.451758423447497 98-99 22.558813496120607 26.631286558651034 26.929296444289115 23.88060350093924 100-101 23.354941118715143 25.30317108398267 27.304256630573775 24.037631166728417 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 730.0 1 508.5 2 429.0 3 595.5 4 534.0 5 507.5 6 898.5 7 2075.0 8 3519.5 9 4165.0 10 3556.5 11 2702.5 12 2646.5 13 2849.5 14 3050.0 15 3317.0 16 3535.5 17 3816.5 18 4296.5 19 4864.0 20 5510.0 21 6690.5 22 8335.0 23 10349.0 24 12908.0 25 16121.0 26 20604.5 27 25833.0 28 30002.5 29 33709.0 30 37802.0 31 44504.0 32 54514.0 33 58422.0 34 57268.5 35 59031.0 36 63244.0 37 68743.0 38 73292.0 39 76165.5 40 79552.5 41 85305.0 42 89753.0 43 92157.5 44 94672.5 45 101121.5 46 105601.5 47 107355.5 48 109397.5 49 106568.5 50 103064.5 51 100964.5 52 99424.5 53 101436.0 54 104735.0 55 100620.0 56 92424.0 57 85103.5 58 79985.5 59 71252.0 60 57105.0 61 47489.5 62 41475.5 63 32232.0 64 22884.0 65 17440.5 66 13324.0 67 10137.5 68 7842.5 69 5920.0 70 4445.5 71 3203.5 72 2164.0 73 1601.5 74 1214.5 75 884.0 76 671.0 77 485.5 78 326.5 79 189.0 80 111.5 81 77.5 82 47.5 83 30.5 84 16.5 85 14.5 86 13.0 87 8.0 88 6.5 89 7.5 90 8.0 91 5.5 92 3.0 93 2.0 94 1.5 95 3.5 96 4.0 97 1.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1488720669095749 2 0.05135017209156568 3 0.15111049340933738 4 0.12835872555354283 5 0.19323968469591143 6 0.19541129249418843 7 0.1646078711093976 8 0.07149601058942781 9 0.12194413021093994 10-11 0.10283398158610224 12-13 0.15077639990191014 14-15 0.13704515674665088 16-17 0.13450604609020392 18-19 0.13121522504204566 20-21 0.13285228322843912 22-23 0.19544470184493118 24-25 0.21231642397000644 26-27 0.14638307027924202 28-29 0.12687200944549165 30-31 0.0980230350791501 32-33 0.12391528190476063 34-35 0.09356288675499654 36-37 0.08678078855422372 38-39 0.05210188248327695 40-41 0.05951875834816152 42-43 0.06185741290015215 44-45 0.040558951801666056 46-47 0.05180119832659244 48-49 0.04364931674536796 50-51 0.05340484716224316 52-53 0.02741237228440445 54-55 0.051316762740822953 56-57 0.027195211504576745 58-59 0.05188472170344925 60-61 0.038404048678760405 62-63 0.0523858619645901 64-65 0.04015803959275338 66-67 0.03943973855178483 68-69 0.06583312563853622 70-71 0.054540765087495745 72-73 0.05155062819602201 74-75 0.04438432246170787 76-77 0.02221721824391098 78-79 0.02283529123265136 80-81 0.026226340333037772 82-83 0.022484493049852766 84-85 0.019811744990434903 86-87 0.02499019435555701 88-89 0.06473061706402634 90-91 0.1091650535518483 92-93 0.08080051477127624 94-95 0.07540490462632644 96-97 0.06730313707121605 98-99 0.05554304560977744 100-101 0.13017953516902125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2993174.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.47915282552706 #Duplication Level Percentage of deduplicated Percentage of total 1 72.82436296414403 36.032877845245345 2 14.468160731858912 14.31744671911873 3 5.259814073085479 7.807534330681632 4 2.4981213631313737 4.944197148123648 5 1.393642083931573 3.4478114827468156 6 0.8169854331632578 2.4254248262228555 7 0.5382066688354498 1.8641007013318938 8 0.3905687442968931 1.5460008470352138 9 0.272452149186224 1.2132631374505625 >10 1.3920470486078278 12.188073199422417 >50 0.09080380049583295 3.0730299696241756 >100 0.0465150917593506 4.399239897380071 >500 0.004126103233749936 1.3865210817962708 >1k 0.003923180123562851 4.151765151751277 >5k 1.352820732263052E-4 0.40603634651108406 >10k+ 1.352820732263052E-4 0.7966773155580155 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13084 0.4371279451177913 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10720 0.3581482399619935 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6666 0.2227067320509934 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5466 0.18261551115972544 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4866 0.16256990071409147 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4863 0.1624696726618633 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4723 0.15779236355788204 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4346 0.14519703832787537 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3909 0.13059715205330527 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3850 0.12862600035948463 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3695 0.12344755099436251 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3453 0.11536248811462348 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3213 0.10734424393636988 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.336374029708931E-4 2 0.0 0.0 0.0 0.0 1.336374029708931E-4 3 0.0 0.0 0.0 0.0 1.336374029708931E-4 4 0.0 0.0 0.0 0.0 1.336374029708931E-4 5 0.0 0.0 0.0 0.0 1.6704675371361638E-4 6 0.0 0.0 0.0 0.0 1.6704675371361638E-4 7 0.0 0.0 0.0 0.0 1.6704675371361638E-4 8 0.0 0.0 0.0 0.0 1.6704675371361638E-4 9 0.0 0.0 0.0 0.0 1.6704675371361638E-4 10-11 0.0 0.0 0.0 0.0 2.0045610445633966E-4 12-13 0.0 0.0 0.0 0.0 4.677309103981259E-4 14-15 0.0 0.0 0.0 0.0 5.345496118835724E-4 16-17 0.0 0.0 0.0 3.3409350742723275E-5 5.846636379976573E-4 18-19 0.0 0.0 0.0 5.011402611408491E-5 6.347776641117423E-4 20-21 1.6704675371361637E-5 0.0 0.0 6.681870148544655E-5 6.848916902258272E-4 22-23 3.3409350742723275E-5 1.6704675371361637E-5 0.0 2.0045610445633963E-4 7.015963655971888E-4 24-25 3.3409350742723275E-5 3.3409350742723275E-5 0.0 4.8443558576948746E-4 7.35005716339912E-4 26-27 3.3409350742723275E-5 3.3409350742723275E-5 0.0 0.0011693272759953147 7.851197424539971E-4 28-29 3.3409350742723275E-5 3.3409350742723275E-5 0.0 0.003992417413755432 8.018244178253586E-4 30-31 3.3409350742723275E-5 3.3409350742723275E-5 0.0 0.01110860912195549 8.018244178253586E-4 32-33 3.3409350742723275E-5 3.3409350742723275E-5 0.0 0.02796362657165938 8.519384439394436E-4 34-35 3.3409350742723275E-5 3.3409350742723275E-5 0.0 0.05375564534504175 8.853477946821667E-4 36-37 3.3409350742723275E-5 3.3409350742723275E-5 0.0 0.09372993350871014 9.020524700535284E-4 38-39 3.3409350742723275E-5 3.3409350742723275E-5 0.0 0.15283107497258763 9.020524700535284E-4 40-41 3.3409350742723275E-5 3.3409350742723275E-5 0.0 0.21697702839861632 9.020524700535284E-4 42-43 3.3409350742723275E-5 3.3409350742723275E-5 0.0 0.2808222976679605 9.187571454248901E-4 44-45 3.3409350742723275E-5 3.3409350742723275E-5 0.0 0.36237452283094806 9.855758469103368E-4 46-47 3.3409350742723275E-5 3.3409350742723275E-5 0.0 0.45580377218297363 0.0010858038991385065 48-49 3.3409350742723275E-5 3.3409350742723275E-5 0.0 0.5502854160833951 0.0011359179252525913 50-51 3.3409350742723275E-5 3.3409350742723275E-5 0.0 0.6555415755983447 0.0012361459774807612 52-53 3.3409350742723275E-5 3.3409350742723275E-5 0.0 0.7537483621065799 0.0014031927311943776 54-55 3.3409350742723275E-5 3.3409350742723275E-5 0.0 0.8620948865652314 0.0014031927311943776 56-57 3.3409350742723275E-5 3.3409350742723275E-5 0.0 0.988916781984609 0.0014366020819371007 58-59 3.3409350742723275E-5 3.3409350742723275E-5 0.0 1.1176430103963217 0.001470011432679824 60-61 3.3409350742723275E-5 3.3409350742723275E-5 0.0 1.2614702653437455 0.001470011432679824 62-63 3.3409350742723275E-5 3.3409350742723275E-5 0.0 1.4001691849521611 0.0015368301341652708 64-65 3.3409350742723275E-5 3.3409350742723275E-5 0.0 1.543311548209359 0.0015869441602793556 66-67 3.3409350742723275E-5 3.3409350742723275E-5 0.0 1.7089216998410381 0.0016036488356507173 68-69 3.3409350742723275E-5 3.3409350742723275E-5 0.0 1.8908523193105378 0.0016704675371361638 70-71 3.3409350742723275E-5 3.3409350742723275E-5 0.0 2.078445823730929 0.0017038768878788872 72-73 3.3409350742723275E-5 3.3409350742723275E-5 0.0 2.2740408676542025 0.001753990913992972 74-75 3.3409350742723275E-5 3.3409350742723275E-5 0.0 2.465977587671148 0.0018375142908497802 76-77 3.3409350742723275E-5 3.3409350742723275E-5 0.0 2.6726812407163765 0.0018709236415925035 78-79 3.3409350742723275E-5 3.3409350742723275E-5 0.0 2.9082171634525755 0.0019210376677065884 80-81 3.3409350742723275E-5 3.3409350742723275E-5 0.0 3.1514038275088585 0.0019544470184493117 82-83 3.3409350742723275E-5 3.3409350742723275E-5 0.0 3.4069853606906917 0.00203797039530612 84-85 3.3409350742723275E-5 3.3409350742723275E-5 0.0 3.670351272595579 0.00203797039530612 86-87 3.3409350742723275E-5 3.3409350742723275E-5 0.0 3.9351537865824042 0.0021047890967915662 88-89 3.3409350742723275E-5 3.3409350742723275E-5 0.0 4.215825742172022 0.002171607798277013 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7100 0.0 46.61866 1 GTATCAA 12365 0.0 40.24904 1 TCAACGC 15530 0.0 31.696064 4 TATCAAC 15670 0.0 31.564636 2 ATCAACG 15740 0.0 31.273184 3 CAACGCA 15855 0.0 30.956364 5 AACGCAG 16385 0.0 29.977024 6 GTGTAAG 1355 0.0 27.063549 1 ACGCAGA 18270 0.0 26.618998 7 CGCAGAG 18360 0.0 26.382734 8 TAAGGTG 2115 0.0 24.285656 5 GCAGAGT 19780 0.0 24.08018 9 GTACATG 9405 0.0 23.698475 1 AGGTAAG 2150 0.0 22.784277 2 TACATGG 9255 0.0 22.66202 2 GAGTACT 12970 0.0 22.539785 12-13 AGAGTAC 16960 0.0 22.141582 10-11 GTACTTT 13365 0.0 21.78307 14-15 ACATGGG 9335 0.0 21.601704 3 GGTAAGG 2370 0.0 21.47197 3 >>END_MODULE