FastQCFastQC Report
Thu 26 May 2016
SRR938236_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938236_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2993174
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127080.4245660292385274No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108110.3611884908795813No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67930.2269497195953192No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA55270.18465348155503156No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC53270.17797161140648687No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA49180.16430718695271307No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA47760.15956305914724636No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA46720.15608848667000313No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG38380.12822508815057193No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA37940.12675507671789213No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA36480.12187731150945451No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA36250.12110889644237188No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT31480.10517263613809287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78650.048.7369961
GTATCAA126650.036.3039671
TATCAAC158000.029.0690942
ATCAACG158050.028.9072283
TCAACGC158800.028.8290714
CAACGCA161900.028.2477285
AACGCAG166700.027.433446
TAAGGTG24150.025.3674415
ACGCAGA184350.024.5767157
CGCAGAG185150.024.4705248
GTGTAAG14300.024.2477111
GAGTACT130500.022.98396712-13
AAGGTAA22850.022.866011
AGGTAAG24450.022.5341552
GTAAGGT26850.022.2859084
GCAGAGT202650.022.1464359
GTACATG91850.022.0817051
GTACTTT135050.022.04950314-15
TACATGG88750.021.9420722
GGTAAGG26550.021.1078173
Produced by FastQC (version 0.11.5)