##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938236_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2993174 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.54853309563694 34.0 31.0 34.0 31.0 34.0 2 32.76170045577036 34.0 31.0 34.0 31.0 34.0 3 32.81240115008349 34.0 31.0 34.0 31.0 34.0 4 36.265825174213056 37.0 35.0 37.0 35.0 37.0 5 36.2397237848518 37.0 37.0 37.0 35.0 37.0 6 36.26941400666984 37.0 37.0 37.0 35.0 37.0 7 36.23228920203102 37.0 37.0 37.0 35.0 37.0 8 36.21380547873261 37.0 37.0 37.0 35.0 37.0 9 37.909564896661536 39.0 38.0 39.0 35.0 39.0 10-11 37.88843415050378 39.0 38.0 39.0 35.0 39.0 12-13 37.81248985190971 39.0 38.0 39.0 35.0 39.0 14-15 39.10647626900408 40.0 38.5 41.0 36.0 41.0 16-17 39.002673917386694 40.0 38.0 41.0 36.0 41.0 18-19 39.2495138939467 40.5 39.0 41.0 36.0 41.0 20-21 39.22339646809708 41.0 39.0 41.0 36.0 41.0 22-23 39.1388848426453 40.0 39.0 41.0 36.0 41.0 24-25 39.04409867251286 40.0 39.0 41.0 35.0 41.0 26-27 38.87502697805073 40.0 38.0 41.0 35.0 41.0 28-29 38.76427147235677 40.0 38.0 41.0 35.0 41.0 30-31 38.51224519523422 40.0 38.0 41.0 34.0 41.0 32-33 38.28544965979258 40.0 38.0 41.0 34.0 41.0 34-35 38.129951516350204 40.0 38.0 41.0 34.0 41.0 36-37 38.10796599195369 40.0 38.0 41.0 34.0 41.0 38-39 37.952452146116464 40.0 38.0 41.0 33.0 41.0 40-41 37.767362839581 40.0 37.0 41.0 33.0 41.0 42-43 37.52995398864216 40.0 37.0 41.0 33.0 41.0 44-45 37.2527529305012 40.0 37.0 41.0 32.0 41.0 46-47 36.86170499944207 40.0 36.0 41.0 31.0 41.0 48-49 36.45173351098199 39.0 36.0 41.0 30.0 41.0 50-51 36.06647258061175 39.0 35.0 41.0 29.5 41.0 52-53 35.54312946724781 39.0 35.0 40.0 28.0 41.0 54-55 35.22451835409502 38.0 35.0 40.0 27.0 41.0 56-57 34.87649381559508 38.0 34.0 40.0 26.0 41.0 58-59 34.43768955630378 38.0 34.0 40.0 25.5 41.0 60-61 33.96138263261675 37.0 33.0 40.0 24.5 41.0 62-63 33.507669617603256 36.5 33.0 39.0 23.0 41.0 64-65 33.02508190302335 36.0 32.0 39.0 22.0 40.0 66-67 32.48312677445414 35.0 31.5 39.0 20.5 40.0 68-69 31.91960106562465 35.0 31.0 38.0 18.5 40.0 70-71 31.312912647243362 35.0 30.5 37.5 14.5 39.5 72-73 30.744737024977496 34.5 30.0 37.0 9.5 39.0 74-75 30.16395889447122 34.0 29.0 36.0 7.0 39.0 76-77 28.837568915138245 32.5 28.0 35.0 7.0 37.0 78-79 29.522584888148835 34.0 29.0 35.0 2.0 37.0 80-81 29.212373553959775 34.0 29.0 35.0 2.0 37.0 82-83 28.663344997651322 34.0 28.5 35.0 2.0 36.5 84-85 28.08075390872699 33.0 27.0 35.0 2.0 36.0 86-87 27.463755197659744 33.0 26.0 35.0 2.0 35.5 88-89 26.728625699675327 32.0 25.0 35.0 2.0 35.0 90-91 26.196826178498142 32.0 24.5 34.5 2.0 35.0 92-93 25.407216720444588 32.0 21.5 34.0 2.0 35.0 94-95 24.602433403470698 31.0 18.0 34.0 2.0 35.0 96-97 23.57317533160451 31.0 4.5 34.0 2.0 35.0 98-99 22.137517230872646 30.5 2.0 34.0 2.0 35.0 100-101 17.726527592448683 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1322606547990759 1101 2 0.16194337854669527 1101 3 0.14758828067478902 1101 4 0.06015766805877831 1101 5 0.10378419038109143 1101 6 0.041942000819460645 1101 7 0.10600313233949521 1101 8 0.11099134181978343 1101 9 0.08532495149034958 1101 10-11 0.145848051091086 1101 12-13 0.20637402544876693 1101 14-15 0.1866714230024158 1101 16-17 0.22865015675388634 1101 18-19 0.24177435536009284 1101 20-21 0.3058421966727991 1101 22-23 0.24823981415484297 1101 24-25 0.32322969106936483 1101 26-27 0.3494200911004839 1101 28-29 0.28627826663787204 1101 30-31 0.30740343957712213 1101 32-33 0.39248872310992766 1101 34-35 0.264233128104415 1101 36-37 0.34703206301770706 1101 38-39 0.41557436037135886 1101 40-41 0.36405162478946096 1101 42-43 0.3230926617144263 1101 44-45 0.4337750135944418 1101 46-47 0.48931289300718817 1101 48-49 0.6387824572670837 1101 50-51 0.5467951555761559 1101 52-53 0.458606068509944 1101 54-55 0.4682405342870126 1101 56-57 0.5607528102590251 1101 58-59 0.6838098812356677 1101 60-61 0.61607140108746 1101 62-63 0.5482138471939138 1101 64-65 0.7284924346524662 1101 66-67 0.4360938959470033 1101 68-69 0.5204013032244141 1101 70-71 0.53718379700857 1101 72-73 0.5157859108896794 1101 74-75 0.5753395265268004 1101 76-77 0.46171482400706765 1101 78-79 0.37204463646448716 1101 80-81 0.44037093693966156 1101 82-83 0.5914716860044145 1101 84-85 0.5094327341423828 1101 86-87 0.33361629535892945 1101 88-89 0.45175467231096533 1101 90-91 0.17743234038155364 1101 92-93 0.07032431435679953 1101 94-95 -0.14490601399123904 1101 96-97 -0.15072229471248733 1101 98-99 0.07575420969711288 1101 100-101 -0.4664753561816859 1102 1 0.12174305188548828 1102 2 0.13259231354282974 1102 3 0.0797977498283089 1102 4 0.07092358793516951 1102 5 0.055535284815114494 1102 6 0.026103057362149684 1102 7 0.0633974596764375 1102 8 0.09320081097329336 1102 9 0.15617437944164436 1102 10-11 0.10789326317216563 1102 12-13 0.1319157243046689 1102 14-15 0.21760987718332814 1102 16-17 0.1585732704646574 1102 18-19 0.1965282456591737 1102 20-21 0.15813866873254057 1102 22-23 0.20720589589711125 1102 24-25 0.16412749484378963 1102 26-27 0.16212840144150675 1102 28-29 0.07259140352489624 1102 30-31 0.0010262920952399668 1102 32-33 1.2768863985712642E-4 1102 34-35 0.08920185856094776 1102 36-37 0.23023020156017537 1102 38-39 0.13652495449268542 1102 40-41 0.16945205888429626 1102 42-43 0.1317368510975001 1102 44-45 0.07700331804942806 1102 46-47 0.0729892716261773 1102 48-49 0.06029774255567588 1102 50-51 0.06618687098652742 1102 52-53 0.09371716735848423 1102 54-55 0.12551346221991366 1102 56-57 0.05282690068317919 1102 58-59 0.1123534562641737 1102 60-61 0.03280520530332609 1102 62-63 0.18151039282906112 1102 64-65 0.24715575363860154 1102 66-67 0.045162063551757115 1102 68-69 0.050350168323927136 1102 70-71 -0.1401273901304556 1102 72-73 -0.2879222890882467 1102 74-75 -0.3166582294236058 1102 76-77 -0.28262262954846307 1102 78-79 -0.3581461077506489 1102 80-81 -0.4478266497873413 1102 82-83 -0.36651584296589945 1102 84-85 -0.24596678026000163 1102 86-87 -0.3564243550475723 1102 88-89 -0.47322509445502803 1102 90-91 -0.6304760950376469 1102 92-93 -0.8469751007187973 1102 94-95 -0.776092785134967 1102 96-97 -0.886509367515341 1102 98-99 -0.9246522943679274 1102 100-101 -1.3064221979577972 1103 1 0.12597880734075773 1103 2 0.11775398553377414 1103 3 0.1703631369790628 1103 4 0.09375474930291716 1103 5 0.03770657430347768 1103 6 0.06758506469781622 1103 7 0.07213129670295615 1103 8 0.019192393550241604 1103 9 0.08072587277361976 1103 10-11 0.07156143415740956 1103 12-13 0.13283116131766803 1103 14-15 0.13737053840740998 1103 16-17 0.15575056032486856 1103 18-19 0.17987696022740352 1103 20-21 0.1608116231128065 1103 22-23 0.18061089564356791 1103 24-25 0.20417482172037893 1103 26-27 0.18432572071198905 1103 28-29 0.16637569438236 1103 30-31 0.17045549615858846 1103 32-33 0.14600264427091503 1103 34-35 0.12291989179117735 1103 36-37 0.1829276645015412 1103 38-39 0.2239042287012296 1103 40-41 0.2665945296853067 1103 42-43 0.2638278539202048 1103 44-45 0.21126696255698363 1103 46-47 0.20342789035747444 1103 48-49 0.13059404260808094 1103 50-51 0.13518066778519255 1103 52-53 0.0901166061969505 1103 54-55 0.18711175845235317 1103 56-57 0.17969741096832337 1103 58-59 0.07127879811634585 1103 60-61 -0.01628459744501498 1103 62-63 -0.08528743689560514 1103 64-65 -0.03947034713384312 1103 66-67 -0.12109252123941872 1103 68-69 -0.03402452531906164 1103 70-71 -0.113494528375643 1103 72-73 -0.20321698340733008 1103 74-75 -0.4449350240418646 1103 76-77 -0.3464236538372951 1103 78-79 -0.3956580662382194 1103 80-81 -0.3382216601823522 1103 82-83 -0.2804901174279806 1103 84-85 -0.20903389991248744 1103 86-87 -0.38328945654681945 1103 88-89 -0.6202409658611465 1103 90-91 -0.545806622563294 1103 92-93 -0.6838866839718456 1103 94-95 -0.6036549439166414 1103 96-97 -0.7146952269207141 1103 98-99 -1.007620703677798 1103 100-101 -1.6031039078102367 1104 1 0.1524743405308513 1104 2 0.11241416872952215 1104 3 0.1453637023980363 1104 4 0.13735684512258928 1104 5 0.07792859548784037 1104 6 0.09774262811897927 1104 7 0.09780848016256272 1104 8 0.07601813142201053 1104 9 0.1508427260285501 1104 10-11 0.13737808763097092 1104 12-13 0.14756390661526808 1104 14-15 0.22196569842098057 1104 16-17 0.14999553751964356 1104 18-19 0.2200365968514717 1104 20-21 0.1824230671928717 1104 22-23 0.16107736629832914 1104 24-25 0.22866933715631887 1104 26-27 0.2519496417041154 1104 28-29 0.15719592884276068 1104 30-31 0.08035051670214699 1104 32-33 0.1622235723119232 1104 34-35 0.1893752802340103 1104 36-37 0.029983904842296738 1104 38-39 0.15394738901635208 1104 40-41 0.1400200263116389 1104 42-43 0.03074047784120637 1104 44-45 0.05983287156221451 1104 46-47 0.08306545679420907 1104 48-49 0.0243783918108349 1104 50-51 0.07860899150640677 1104 52-53 0.1454517111764062 1104 54-55 0.1716411413917811 1104 56-57 0.19683073676274176 1104 58-59 0.20888292944432152 1104 60-61 0.03052926662826394 1104 62-63 0.05971539019927263 1104 64-65 4.5501756139287863E-4 1104 66-67 -0.13971610298014525 1104 68-69 7.75316363625933E-4 1104 70-71 -0.3505941175045173 1104 72-73 -0.311970988458409 1104 74-75 -0.2213987052963411 1104 76-77 -0.2373494331731152 1104 78-79 -0.2595801891979974 1104 80-81 -0.23721351814910108 1104 82-83 -0.3290556494872341 1104 84-85 -0.47001358252396486 1104 86-87 -0.5089166946636006 1104 88-89 -0.6290803491775172 1104 90-91 -0.7709479773071592 1104 92-93 -0.6269650133472453 1104 94-95 -0.5631377740270871 1104 96-97 -0.677985211465927 1104 98-99 -0.7168377868697782 1104 100-101 -1.1835926182780305 1105 1 0.0790909269382567 1105 2 0.06656162389953835 1105 3 0.04988102332223576 1105 4 0.017628806909129935 1105 5 0.001390040230312195 1105 6 0.06949710590285463 1105 7 0.011724145840332767 1105 8 0.048307354874587816 1105 9 0.12708043944090264 1105 10-11 0.044900034484449236 1105 12-13 0.10419084186838035 1105 14-15 0.08136065360758238 1105 16-17 0.06239952744290633 1105 18-19 0.10129022937690024 1105 20-21 0.0959334070313318 1105 22-23 0.14390043943618025 1105 24-25 0.060584385601053725 1105 26-27 0.10184172073649478 1105 28-29 0.11419773123906651 1105 30-31 0.08484216698985136 1105 32-33 0.14573806970314251 1105 34-35 0.055612182994686066 1105 36-37 0.06949144105851701 1105 38-39 0.1287774840095608 1105 40-41 0.13783778517737488 1105 42-43 0.17940866246499354 1105 44-45 0.12144156952287943 1105 46-47 0.1369698823834895 1105 48-49 0.15966276462717133 1105 50-51 0.1864538716484745 1105 52-53 0.08485751297728683 1105 54-55 0.08398812496162833 1105 56-57 0.22472971723788504 1105 58-59 0.35645282326317584 1105 60-61 0.08604788459515333 1105 62-63 0.054259846621768304 1105 64-65 -0.07025917130811621 1105 66-67 0.12520735443503384 1105 68-69 -0.037552982396125145 1105 70-71 -0.20629136327988462 1105 72-73 -0.06514758157858225 1105 74-75 -0.14558464034749719 1105 76-77 -0.08568100772415121 1105 78-79 -0.15494947185185381 1105 80-81 -0.28458082015809794 1105 82-83 -0.33597593635363765 1105 84-85 -0.556757578723861 1105 86-87 -0.5025866191070705 1105 88-89 -0.6358260614938267 1105 90-91 -0.7965789108270833 1105 92-93 -0.7935791772809253 1105 94-95 -0.7011000232583875 1105 96-97 -0.7942677430126039 1105 98-99 -0.9576392606440116 1105 100-101 -1.5274076541126256 1106 1 0.09718321697714316 1106 2 0.1807759159961364 1106 3 0.1344057653106958 1106 4 0.08266819772443768 1106 5 0.11478657513443835 1106 6 0.11234758278854429 1106 7 0.08658628974365001 1106 8 0.07100991437621929 1106 9 0.06676862574950349 1106 10-11 0.08685424426190735 1106 12-13 0.019847939817921656 1106 14-15 0.1156003823943621 1106 16-17 0.07896485840466028 1106 18-19 0.146774772720903 1106 20-21 0.23413685457025224 1106 22-23 0.2359791155160167 1106 24-25 0.22878658952200226 1106 26-27 0.2222285120506058 1106 28-29 0.09479167377450892 1106 30-31 0.22373273958187667 1106 32-33 0.29666325876703326 1106 34-35 0.30304695967381434 1106 36-37 0.20008439078970497 1106 38-39 0.24680871658650005 1106 40-41 0.2981819433864743 1106 42-43 0.3140954928119868 1106 44-45 0.20885419621646406 1106 46-47 0.0985520152510091 1106 48-49 0.20503587875629137 1106 50-51 0.19072449754885668 1106 52-53 0.17503562206139378 1106 54-55 -0.03885807764046234 1106 56-57 -0.1279296396026126 1106 58-59 -0.02048360225482071 1106 60-61 -0.014442978731757705 1106 62-63 0.1217589099893317 1106 64-65 0.05745869566760575 1106 66-67 0.035800236237506056 1106 68-69 -0.3292169275598873 1106 70-71 -0.7124846330075592 1106 72-73 -0.44715331789708657 1106 74-75 -0.3598523087362473 1106 76-77 -0.412451034023011 1106 78-79 -0.23386748241842525 1106 80-81 -0.299523569316694 1106 82-83 -0.4886489834423422 1106 84-85 -0.49951052565429066 1106 86-87 -0.596410409936798 1106 88-89 -0.5877399790942128 1106 90-91 -0.7951390833869176 1106 92-93 -0.8137102431190009 1106 94-95 -0.7994871866128328 1106 96-97 -1.030071687162664 1106 98-99 -1.0984855269945832 1106 100-101 -1.7089835845661625 1107 1 0.055448705450451996 1107 2 0.08650770146633135 1107 3 0.09803962959257717 1107 4 0.05238728612185639 1107 5 -0.017671599840440422 1107 6 0.060919646130635385 1107 7 0.03069995352097976 1107 8 0.027292332279905906 1107 9 0.053993624152205655 1107 10-11 0.04330389450188221 1107 12-13 0.0508678440961674 1107 14-15 0.10080661302106364 1107 16-17 0.03447585362825123 1107 18-19 0.027114348749421424 1107 20-21 0.04233782185563939 1107 22-23 0.09526012295653175 1107 24-25 0.13452224723153705 1107 26-27 0.09587540641785353 1107 28-29 -0.006643571735146736 1107 30-31 -0.07710129236797059 1107 32-33 -0.08673018301481505 1107 34-35 0.027768134240176323 1107 36-37 5.164616582931103E-5 1107 38-39 -0.040268338072095844 1107 40-41 -0.17246043207746453 1107 42-43 -0.07207628069299687 1107 44-45 -0.0459867079901457 1107 46-47 0.002651288818903197 1107 48-49 -0.15056952650259348 1107 50-51 -0.23434573519208612 1107 52-53 -0.19293323835793075 1107 54-55 -0.14246105113304708 1107 56-57 -0.051270433450397945 1107 58-59 -0.09164640668543456 1107 60-61 -0.1440383587630052 1107 62-63 -0.07914049020079261 1107 64-65 -0.20338659965840122 1107 66-67 -0.2772651107054571 1107 68-69 -0.3605352362819154 1107 70-71 -0.3719539764887436 1107 72-73 -0.4749221142308535 1107 74-75 -0.34691842431871933 1107 76-77 -0.49933876037960445 1107 78-79 -0.5244184197907629 1107 80-81 -0.43498999022523677 1107 82-83 -0.4988030840469939 1107 84-85 -0.5999018396406264 1107 86-87 -0.6757851449304191 1107 88-89 -0.8862308222162412 1107 90-91 -1.04750744177041 1107 92-93 -0.9959404884060028 1107 94-95 -1.0574854179675164 1107 96-97 -1.3810486773484705 1107 98-99 -1.4133023361919292 1107 100-101 -1.780711130902347 1108 1 0.015962278601620028 1108 2 0.04062073456207571 1108 3 0.0036001184815361853 1108 4 0.016749779398764986 1108 5 -0.004258935351451498 1108 6 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Count 2 4.0 3 7.0 4 19.0 5 46.0 6 92.0 7 255.0 8 671.0 9 1314.0 10 2432.0 11 3737.0 12 6324.0 13 8988.0 14 12393.0 15 17331.0 16 22724.0 17 31411.0 18 31907.0 19 25484.0 20 23321.0 21 23518.0 22 24944.0 23 27158.0 24 31061.0 25 35052.0 26 40554.0 27 47691.0 28 57614.0 29 69064.0 30 83894.0 31 102995.0 32 128917.0 33 167776.0 34 228440.0 35 330090.0 36 472896.0 37 589186.0 38 326243.0 39 17621.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.98012842316428 18.79136013749792 15.21388156975901 23.014629869578783 2 15.633384813092388 19.86466013668979 39.932978298975996 24.56897675124183 3 19.272883157377372 23.494757072514215 30.28050823134436 26.95185153876405 4 13.077798402497418 16.937917366593034 36.659570515827355 33.3247137150822 5 13.541762719384407 37.22321782952263 34.460207113046195 14.774812338046766 6 32.38117256830827 36.41084459637796 16.75548140035722 14.452501434956543 7 28.447360561063274 33.10559292577044 20.131706342497964 18.315340170668325 8 26.713715941672618 34.49261553120533 19.394762883814973 19.39890564330707 9 26.015694376604902 16.85809110997222 20.19581888657325 36.930395626849624 10-11 25.038120069197443 26.415888284476612 26.90105887596244 21.6449327703635 12-13 26.173966237508118 23.686615144476843 27.398113822789682 22.741304795225357 14-15 22.53806494376872 25.196847894576123 26.007241810866994 26.257845350788163 16-17 21.972257038602496 28.34605182247672 27.05910142500201 22.62258971391877 18-19 22.062215561140114 26.428433495680505 29.657530768341566 21.851820174837812 20-21 22.321104620045475 25.626826238634976 30.150335396472105 21.901733744847444 22-23 22.195218186446898 25.549984731926713 30.141899535409568 22.112897546216825 24-25 22.129660364170807 25.81762526045551 30.032139204065654 22.02057517130802 26-27 21.547534473528916 26.20076749674028 29.81640293712528 22.435295092605525 28-29 21.81285662641022 26.743347261490065 29.53038746024862 21.9134086518511 30-31 21.91066203708731 26.14844779069 29.4134204901485 22.527469682074187 32-33 21.48064228808616 26.20861667246876 29.45049302178891 22.860248017656172 34-35 22.39607096204996 26.383363535412446 29.155127534523007 22.065437968014585 36-37 22.580865557221212 25.744826431845812 29.279317053255323 22.394990957677656 38-39 21.943086547557815 26.23764716612957 29.13777858556471 22.68148770074791 40-41 22.52158041794029 26.068947752285503 29.19366063425063 22.21581119552358 42-43 22.199824261296055 26.14554238443343 28.906678070749862 22.747955283520653 44-45 22.210569783113176 26.31858355043843 29.223977623753246 22.246869042695145 46-47 22.79856232641725 26.74419653551136 28.24422877558868 22.213012362482708 48-49 22.36223610834786 26.81534587828798 28.134037807257258 22.6883802061069 50-51 22.590150515360584 27.05910358998346 28.129633651292156 22.22111224336379 52-53 23.03996664128455 26.623723111969877 28.119786656580644 22.216523590164925 54-55 22.298867780036623 26.955298419556385 28.282377894137355 22.463455906269637 56-57 22.497953747253078 27.033726288487635 28.0755233085689 22.392796655690386 58-59 22.531184961710533 26.569122877838623 27.918544158199854 22.981148002250983 60-61 21.906536485557762 27.01196200144267 28.38872979869104 22.69277171430853 62-63 22.162741525462668 26.96813020926011 28.491571200983774 22.377557064293455 64-65 21.79669834940862 27.06364022324228 28.223794320097 22.9158671072521 66-67 21.711260429905142 27.101121650748063 28.150063995757783 23.037553923589016 68-69 22.542032224817092 26.604573581959663 28.286781622256058 22.566612570967184 70-71 22.21925014861296 26.964389360280926 27.73431978702651 23.0820407040796 72-73 22.224720636536567 27.051902107226855 27.83820098131997 22.885176274916603 74-75 22.262091766000808 27.52475459262727 27.50365308384433 22.709500557527594 76-77 22.259589221796404 26.949201505945037 27.943173348254884 22.848035924003675 78-79 22.350174743499 27.32578617865476 27.724331018792363 22.599708059053878 80-81 22.672089194716825 27.39279937370169 27.31073638501174 22.624375046569746 82-83 22.510381885963103 27.514308044438867 27.505785631914332 22.469524437683702 84-85 22.41873937552569 27.73442682224668 27.457918978139023 22.388914824088612 86-87 22.72393682456515 27.62835789520824 27.049961583951017 22.5977436962756 88-89 22.392596419446228 27.833001057978983 26.75752832583347 23.01687419674132 90-91 22.28651614425395 27.813773462454392 27.005975937178135 22.89373445611352 92-93 22.554645724075165 27.354082436892103 27.23940991857967 22.85186192045306 94-95 22.203931595543732 27.19240532637603 27.416982961036567 23.186680117043675 96-97 22.561800478528614 26.719262155056168 27.4708203970608 23.24811696935442 98-99 22.249286838994767 27.211901748758493 26.897434291036515 23.641377121210223 100-101 22.644623763787187 26.59298687660183 27.082184418552085 23.680204941058896 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 744.0 1 513.5 2 429.5 3 593.5 4 496.5 5 472.0 6 922.5 7 2194.0 8 3589.5 9 4183.5 10 3567.0 11 2642.5 12 2488.0 13 2699.0 14 2943.5 15 3167.0 16 3412.0 17 3729.0 18 4086.5 19 4662.0 20 5380.5 21 6486.5 22 8063.5 23 9892.0 24 12448.5 25 15971.5 26 20471.0 27 25581.0 28 29886.5 29 33695.0 30 37920.0 31 44477.5 32 54895.0 33 59052.5 34 57497.5 35 59433.5 36 63387.5 37 68175.5 38 73131.5 39 76799.0 40 80600.5 41 86667.0 42 91013.5 43 92952.5 44 96020.0 45 102278.5 46 105938.5 47 108951.0 48 111432.0 49 107818.5 50 104129.0 51 102028.0 52 100565.0 53 102927.0 54 106555.5 55 101392.5 56 92603.0 57 85221.5 58 79707.0 59 70790.5 60 55495.5 61 45901.5 62 40663.0 63 30618.0 64 20507.0 65 15251.0 66 11597.5 67 9004.5 68 6939.5 69 5191.5 70 3804.0 71 2584.5 72 1757.0 73 1343.0 74 1036.0 75 735.0 76 521.0 77 337.5 78 207.0 79 108.5 80 67.5 81 40.0 82 22.5 83 14.0 84 7.5 85 5.0 86 5.5 87 2.0 88 1.0 89 1.0 90 0.0 91 1.5 92 3.0 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00855279379013716 2 0.01376465250600199 3 0.009521664961676134 4 6.681870148544656E-5 5 8.686431193108053E-4 6 0.0017372862386216105 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0010022805222816983 14-15 0.0 16-17 0.0030235462422164567 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0033576397496436895 26-27 0.019644698236721287 28-29 0.006548232745573763 30-31 0.0033910491003864124 32-33 0.0 34-35 0.009304504181848432 36-37 9.020524700535284E-4 38-39 0.00285649948850284 40-41 0.0022384264997624592 42-43 0.003140478969815988 44-45 0.0 46-47 1.6704675371361638E-4 48-49 6.180729887403807E-4 50-51 0.004142759492097687 52-53 0.00845256573790899 54-55 0.008302223659566734 56-57 0.016988654852674784 58-59 0.02358700162436263 60-61 0.00377525663392773 62-63 0.02026277122546167 64-65 0.02139868915071426 66-67 0.013280216920232504 68-69 0.03160524580261622 70-71 0.01673808472210436 72-73 0.005495838197177979 74-75 0.016604447319133468 76-77 0.021198233046257918 78-79 0.026326568385265946 80-81 0.01172668211069587 82-83 0.0355308445148862 84-85 0.022484493049852766 86-87 0.031237742944446267 88-89 0.022584721102080936 90-91 0.028063854623887552 92-93 0.024706214874243865 94-95 0.014867161080511858 96-97 0.015318187315538622 98-99 0.011242246524926383 100-101 0.010473831457843748 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2993174.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.159699751451036 #Duplication Level Percentage of deduplicated Percentage of total 1 70.6000449287544 33.29476921279011 2 15.46591383626298 14.587357057999487 3 5.721959313191386 8.095376496003743 4 2.766729863901478 5.219125986998669 5 1.4893782854632636 3.511931637938922 6 0.9372485574191449 2.6520216336220495 7 0.6014448532619032 1.985477108782082 8 0.4373883614382416 1.6501683040165294 9 0.30723914388262563 1.3040375199657739 >10 1.5111607186217986 12.675248086615056 >50 0.10068845005713148 3.2986582995303224 >100 0.05144470591421469 4.6486796328586415 >500 0.004537688160895993 1.39896548653804 >1k 0.00446678678300309 4.3017210679509 >5k 2.1270413252395663E-4 0.5900605640497585 >10k+ 1.418027550159711E-4 0.7864019043399031 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12708 0.4245660292385274 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10811 0.3611884908795813 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6793 0.2269497195953192 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5527 0.18465348155503156 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5327 0.17797161140648687 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4918 0.16430718695271307 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4776 0.15956305914724636 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4672 0.15608848667000313 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3838 0.12822508815057193 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3794 0.12675507671789213 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3648 0.12187731150945451 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3625 0.12110889644237188 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3148 0.10517263613809287 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0022805222816983E-4 2 0.0 0.0 0.0 0.0 1.0022805222816983E-4 3 0.0 0.0 0.0 0.0 1.0022805222816983E-4 4 0.0 0.0 0.0 0.0 1.0022805222816983E-4 5 0.0 0.0 0.0 0.0 1.0022805222816983E-4 6 0.0 0.0 0.0 0.0 1.336374029708931E-4 7 0.0 0.0 0.0 0.0 1.336374029708931E-4 8 0.0 0.0 0.0 0.0 1.336374029708931E-4 9 0.0 0.0 0.0 0.0 1.336374029708931E-4 10-11 0.0 0.0 0.0 0.0 1.336374029708931E-4 12-13 0.0 0.0 0.0 3.3409350742723275E-5 2.6727480594178625E-4 14-15 0.0 0.0 0.0 3.3409350742723275E-5 3.3409350742723276E-4 16-17 0.0 0.0 0.0 6.681870148544655E-5 4.343215596554026E-4 18-19 0.0 0.0 0.0 8.352337685680819E-5 5.011402611408491E-4 20-21 0.0 0.0 0.0 1.0022805222816983E-4 6.514823394831038E-4 22-23 0.0 0.0 0.0 2.3386545519906294E-4 6.681870148544655E-4 24-25 0.0 0.0 0.0 5.178449365122108E-4 6.681870148544655E-4 26-27 0.0 0.0 0.0 0.001202736626738038 7.517103917112737E-4 28-29 0.0 0.0 0.0 0.004009122089126793 7.684150670826354E-4 30-31 0.0 0.0 0.0 0.011192132498812297 7.851197424539971E-4 32-33 0.0 0.0 0.0 0.028264310728343893 9.020524700535284E-4 34-35 0.0 0.0 0.0 0.05444053703526758 9.020524700535284E-4 36-37 0.0 0.0 0.0 0.0948825561093341 9.020524700535284E-4 38-39 0.0 0.0 0.0 0.1544848378343524 9.855758469103366E-4 40-41 0.0 0.0 0.0 0.2190317034692938 0.0010022805222816983 42-43 0.0 0.0 0.0 0.28337811299977883 0.00101898519765306 44-45 0.0 0.0 0.0 0.36509738491648 0.0011192132498812298 46-47 0.0 0.0 0.0 0.4589275464774183 0.001202736626738038 48-49 0.0 0.0 0.0 0.5538101025867523 0.0013530787050802927 50-51 0.0 0.0 0.0 0.6597678584672992 0.001436602081937101 52-53 0.0 0.0 0.0 0.7590270395239301 0.0015034207834225474 54-55 0.0 0.0 0.0 0.8688268707398901 0.0015034207834225474 56-57 0.0 0.0 0.0 0.9960830877189231 0.0015535348095366323 58-59 0.0 0.0 0.0 1.1257280732760608 0.0016036488356507173 60-61 0.0 0.0 0.0 1.271008634980793 0.0016036488356507173 62-63 0.0 0.0 0.0 1.4103924462794344 0.0016704675371361638 64-65 0.0 0.0 0.0 1.5546039087603996 0.0016871722125075255 66-67 0.0 0.0 0.0 1.722769875723897 0.0017038768878788872 68-69 0.0 0.0 0.0 1.9074901759804141 0.0017038768878788872 70-71 0.0 0.0 0.0 2.097221878848339 0.0017038768878788872 72-73 0.0 0.0 0.0 2.295021939920633 0.001753990913992972 74-75 0.0 0.0 0.0 2.4904833464409353 0.0018041049401070568 76-77 0.0 0.0 0.0 2.700010089623924 0.0018542189662211419 78-79 0.0 0.0 0.0 2.939404792370908 0.001887628316963865 80-81 0.0 0.0 0.0 3.1870516047513444 0.0019210376677065884 82-83 0.0 0.0 0.0 3.4479619293766417 0.00203797039530612 84-85 0.0 0.0 0.0 3.718109939482302 0.002054675070677482 86-87 0.0 0.0 0.0 3.9894439815393294 0.0021549031229056515 88-89 0.0 0.0 0.0 4.278969415075769 0.0022384264997624597 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7865 0.0 48.736996 1 GTATCAA 12665 0.0 36.303967 1 TATCAAC 15800 0.0 29.069094 2 ATCAACG 15805 0.0 28.907228 3 TCAACGC 15880 0.0 28.829071 4 CAACGCA 16190 0.0 28.247728 5 AACGCAG 16670 0.0 27.43344 6 TAAGGTG 2415 0.0 25.367441 5 ACGCAGA 18435 0.0 24.576715 7 CGCAGAG 18515 0.0 24.470524 8 GTGTAAG 1430 0.0 24.247711 1 GAGTACT 13050 0.0 22.983967 12-13 AAGGTAA 2285 0.0 22.86601 1 AGGTAAG 2445 0.0 22.534155 2 GTAAGGT 2685 0.0 22.285908 4 GCAGAGT 20265 0.0 22.146435 9 GTACATG 9185 0.0 22.081705 1 GTACTTT 13505 0.0 22.049503 14-15 TACATGG 8875 0.0 21.942072 2 GGTAAGG 2655 0.0 21.107817 3 >>END_MODULE