FastQCFastQC Report
Thu 26 May 2016
SRR938235_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938235_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293461
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91580.39930916636472125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75880.3308536748608326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45920.20022141209290237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44650.053.829791
GTATCAA80250.041.894571
ATCAACG101000.032.608813
TATCAAC102000.032.5686762
TCAACGC102500.032.2707064
CAACGCA104350.031.5164095
AACGCAG108750.030.3233726
GTACGCT953.4797693E-530.0158583
ACGCAGA121350.026.8177477
CGCAGAG121900.026.6188018
TGGTATC21400.023.7625542
GCAGAGT133500.023.5585299
GTGGTAT23450.023.183321
GAGTACT81300.022.93515612-13
GTACATG78400.022.3843941
GGACCGA8950.022.2983916
TACATGG76000.022.1992282
CAGAGTA124450.021.68256810-11
GTACTTT87350.021.23785614-15
AGAGTAC117000.021.07360310-11
Produced by FastQC (version 0.11.5)