##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938235_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2293461 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.88824793619774 33.0 31.0 34.0 30.0 34.0 2 32.050541081797334 33.0 31.0 34.0 30.0 34.0 3 32.13661841208549 34.0 31.0 34.0 30.0 34.0 4 35.6611470611447 37.0 35.0 37.0 35.0 37.0 5 35.668047985119436 37.0 35.0 37.0 35.0 37.0 6 35.70062931089738 37.0 35.0 37.0 35.0 37.0 7 35.63410539791172 37.0 35.0 37.0 33.0 37.0 8 35.603431669428865 37.0 35.0 37.0 33.0 37.0 9 37.24244231752796 39.0 37.0 39.0 34.0 39.0 10-11 37.13919486749502 39.0 37.0 39.0 34.0 39.0 12-13 37.00110073814204 39.0 37.0 39.0 33.0 39.0 14-15 38.155842414586516 40.0 38.0 41.0 33.0 41.0 16-17 37.97355176303412 40.0 38.0 41.0 33.0 41.0 18-19 38.15931315160799 40.0 38.0 41.0 33.5 41.0 20-21 38.144345380191766 40.0 38.0 41.0 33.0 41.0 22-23 38.032743308039684 40.0 38.0 41.0 33.0 41.0 24-25 37.8983758607624 40.0 38.0 41.0 33.0 41.0 26-27 37.68621201755774 40.0 38.0 41.0 32.0 41.0 28-29 37.47314757041868 40.0 37.0 41.0 32.0 41.0 30-31 37.219858327654144 40.0 37.0 41.0 31.0 41.0 32-33 36.894185905057896 39.5 36.0 41.0 30.5 41.0 34-35 36.59461290163644 39.0 36.0 41.0 30.0 41.0 36-37 36.27539208209775 39.0 35.0 40.5 30.0 41.0 38-39 35.93155344695201 39.0 35.0 40.0 28.5 41.0 40-41 35.775272830015425 39.0 35.0 40.0 27.5 41.0 42-43 35.49722733458297 38.5 35.0 40.0 26.5 41.0 44-45 35.11798936192942 38.0 34.5 40.0 25.5 41.0 46-47 34.65454960864824 38.0 34.0 40.0 24.0 41.0 48-49 34.164045300966535 38.0 33.0 40.0 22.5 41.0 50-51 33.335216513383045 37.5 32.5 39.5 20.0 40.5 52-53 33.299297655377615 37.5 33.0 39.5 19.0 40.5 54-55 33.262748309214764 38.0 32.5 40.0 16.5 41.0 56-57 32.77454619895433 37.0 32.0 40.0 10.5 41.0 58-59 32.28757388941865 37.0 31.0 40.0 7.5 41.0 60-61 31.70001604561839 36.0 30.5 39.5 2.0 41.0 62-63 31.05593990915913 35.5 29.5 39.0 2.0 41.0 64-65 30.42556620757885 35.0 28.5 39.0 2.0 40.0 66-67 29.723220059115896 35.0 28.0 38.0 2.0 40.0 68-69 28.98317695395736 34.0 26.5 38.0 2.0 40.0 70-71 28.132675462979314 34.0 25.5 37.0 2.0 39.5 72-73 27.30140298875804 33.5 23.0 36.0 2.0 39.0 74-75 26.44235197372007 33.0 21.0 36.0 2.0 39.0 76-77 25.588272048227548 32.0 17.0 35.0 2.0 37.5 78-79 24.730497488293892 31.5 8.5 35.0 2.0 37.0 80-81 23.909811634032582 31.0 4.0 35.0 2.0 36.5 82-83 23.070455961535863 30.5 2.0 34.5 2.0 36.0 84-85 22.240097389927275 29.5 2.0 34.0 2.0 35.5 86-87 21.454527676729626 29.0 2.0 34.0 2.0 35.0 88-89 20.603497508786937 28.5 2.0 34.0 2.0 35.0 90-91 19.738230996733755 26.5 2.0 34.0 2.0 35.0 92-93 18.819405692967962 25.5 2.0 33.5 2.0 35.0 94-95 17.880039163517495 23.5 2.0 33.0 2.0 35.0 96-97 16.778928658477298 13.0 2.0 32.5 2.0 35.0 98-99 15.47506432418079 2.0 2.0 32.0 2.0 34.0 100-101 12.486833872474833 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.40879025997997687 1101 2 0.3518834473635124 1101 3 0.469535342017501 1101 4 0.2734087960122764 1101 5 0.35521808108746455 1101 6 0.34569043801312915 1101 7 0.3801704414161904 1101 8 0.42433370997127184 1101 9 0.38023208707493694 1101 10-11 0.36454783606074415 1101 12-13 0.49125571036044136 1101 14-15 0.703934656654333 1101 16-17 0.7417159034826994 1101 18-19 0.7152371732946534 1101 20-21 0.6334955984697856 1101 22-23 0.6620700850720738 1101 24-25 0.6989800098719243 1101 26-27 0.7860699636587256 1101 28-29 0.8620193826976958 1101 30-31 0.8709110235249327 1101 32-33 0.8592468524852919 1101 34-35 0.9618111248381709 1101 36-37 1.0986077128936742 1101 38-39 1.3429212287215009 1101 40-41 1.4858808964589443 1101 42-43 1.6442167037796196 1101 44-45 1.7399789684195142 1101 46-47 2.0520989048198572 1101 48-49 2.1359204224229984 1101 50-51 2.2613546242787876 1101 52-53 2.164565565801304 1101 54-55 2.3771930277424715 1101 56-57 2.6027752782099824 1101 58-59 2.833486442283572 1101 60-61 3.0689125335679392 1101 62-63 3.3299259306927773 1101 64-65 3.1393338848259695 1101 66-67 3.2490629673100813 1101 68-69 3.4162772264874555 1101 70-71 3.72315791721023 1101 72-73 4.202906627876811 1101 74-75 4.408838701509676 1101 76-77 4.6501753832821 1101 78-79 4.994807251236697 1101 80-81 4.8820625334728085 1101 82-83 5.298583148465145 1101 84-85 5.321308881163045 1101 86-87 5.530905313169498 1101 88-89 5.687017489929158 1101 90-91 5.443936043397969 1101 92-93 5.784909828908376 1101 94-95 5.460017859719734 1101 96-97 5.416782373531895 1101 98-99 5.126947970024551 1101 100-101 4.512602874262463 1102 1 0.47563936971834764 1102 2 0.3971194878189408 1102 3 0.33108678944967096 1102 4 0.22589032459755032 1102 5 0.23726220051575808 1102 6 0.25957882221855755 1102 7 0.31416541476890103 1102 8 0.3542710282852255 1102 9 0.5313645967648597 1102 10-11 0.482181980925084 1102 12-13 0.49615366336431777 1102 14-15 0.6928631631950353 1102 16-17 0.6767726046697149 1102 18-19 0.7223797059981365 1102 20-21 0.6266520907178474 1102 22-23 0.665348004161217 1102 24-25 0.7015936393535114 1102 26-27 0.717856316008529 1102 28-29 0.745458321045561 1102 30-31 0.7123256008020675 1102 32-33 0.8860966435463382 1102 34-35 0.9466554804583254 1102 36-37 0.985390810035149 1102 38-39 1.173701494588748 1102 40-41 1.1232052666139865 1102 42-43 1.3115419823648864 1102 44-45 1.3817716797809467 1102 46-47 1.499917158211332 1102 48-49 1.5530942959695082 1102 50-51 1.7574162007080574 1102 52-53 1.9187310969301805 1102 54-55 1.9568207227521626 1102 56-57 2.3927424233165695 1102 58-59 2.4896039628746536 1102 60-61 2.6461726322840207 1102 62-63 2.7747198684350245 1102 64-65 2.9445683355284835 1102 66-67 3.1257378855513593 1102 68-69 3.3242097149806717 1102 70-71 3.571862647103643 1102 72-73 3.5916220318254553 1102 74-75 3.9505981037625837 1102 76-77 4.16527046564644 1102 78-79 4.309216847288052 1102 80-81 4.437851505129785 1102 82-83 4.824066509051388 1102 84-85 4.839503981695991 1102 86-87 5.141031660674344 1102 88-89 5.236167350636524 1102 90-91 5.361520603112115 1102 92-93 5.431047864882213 1102 94-95 5.445110141090858 1102 96-97 5.309880953352632 1102 98-99 5.436166265809435 1102 100-101 4.511336749625833 1103 1 0.2776211151547052 1103 2 0.26786095035848945 1103 3 0.21217322953795303 1103 4 0.1047520237179782 1103 5 0.07956516756186716 1103 6 0.021157124402797933 1103 7 0.20563420587972558 1103 8 0.17072650084902108 1103 9 0.21182139574721504 1103 10-11 0.1480189564733152 1103 12-13 0.33820384982397655 1103 14-15 0.48524332345248666 1103 16-17 0.5607198142982099 1103 18-19 0.4989719518857001 1103 20-21 0.4793092088017161 1103 22-23 0.4365462385024159 1103 24-25 0.49087757718579184 1103 26-27 0.6122398983171493 1103 28-29 0.6155267212977975 1103 30-31 0.6383944142010662 1103 32-33 0.8342069727590768 1103 34-35 0.7440518404178746 1103 36-37 0.7647381604597072 1103 38-39 0.919299578892435 1103 40-41 0.9709443753041995 1103 42-43 1.0607578189327285 1103 44-45 1.2122958869196836 1103 46-47 1.4980729471585619 1103 48-49 1.4456377443315986 1103 50-51 1.725072177202172 1103 52-53 1.7583268654375246 1103 54-55 1.6511233745020562 1103 56-57 1.8423670577360483 1103 58-59 2.108647650426981 1103 60-61 2.2294373343291056 1103 62-63 2.4958906502609146 1103 64-65 2.5336728127347463 1103 66-67 2.763908977237321 1103 68-69 2.8031551986341974 1103 70-71 3.186841799500435 1103 72-73 3.6134104272938856 1103 74-75 3.800199090965908 1103 76-77 4.0864983949113665 1103 78-79 4.1298781946901215 1103 80-81 4.186834267124635 1103 82-83 4.52536513533839 1103 84-85 4.5346234137279104 1103 86-87 4.5757238935151605 1103 88-89 4.908967683872003 1103 90-91 4.930141698603379 1103 92-93 5.075940405952263 1103 94-95 5.196269398296465 1103 96-97 5.13764631860699 1103 98-99 5.052486106639206 1103 100-101 4.096131725543728 1104 1 0.30815257958027686 1104 2 0.320951361162102 1104 3 0.3039108391108627 1104 4 0.13111853325776224 1104 5 0.12696478581039372 1104 6 0.07720986261400498 1104 7 0.19106573367093205 1104 8 0.2858312986165714 1104 9 0.2418394881623982 1104 10-11 0.41825696594852246 1104 12-13 0.3412198292579447 1104 14-15 0.584857072130383 1104 16-17 0.45989164767664903 1104 18-19 0.42292074535330926 1104 20-21 0.41315128449477356 1104 22-23 0.5082788895896613 1104 24-25 0.536861257883551 1104 26-27 0.6805457425588273 1104 28-29 0.6487278067129125 1104 30-31 0.6340503307756293 1104 32-33 0.7565811441543673 1104 34-35 0.7769204130727871 1104 36-37 0.8343115338243763 1104 38-39 0.9034557893632709 1104 40-41 1.1572262660794905 1104 42-43 1.2644622317469967 1104 44-45 1.3856319547756542 1104 46-47 1.4638743234628464 1104 48-49 1.6602088558739467 1104 50-51 1.672027738396963 1104 52-53 1.702135994892295 1104 54-55 1.8574315331363849 1104 56-57 2.045643660541664 1104 58-59 2.3350800784040047 1104 60-61 2.485126882936001 1104 62-63 2.73550146538447 1104 64-65 2.986827562702242 1104 66-67 2.9830733945211563 1104 68-69 3.2091367289241184 1104 70-71 3.4099667314889217 1104 72-73 3.6557603730685635 1104 74-75 3.892154553726531 1104 76-77 3.9913703234139852 1104 78-79 4.29320437369881 1104 80-81 4.381708919332944 1104 82-83 4.6247207918087945 1104 84-85 4.790208687025494 1104 86-87 4.804432854853033 1104 88-89 4.906962547555835 1104 90-91 5.123962131761285 1104 92-93 5.325517950329303 1104 94-95 5.192420189912646 1104 96-97 5.192686699328689 1104 98-99 5.166009792169971 1104 100-101 4.26555865916384 1105 1 0.46635630253257077 1105 2 0.4261419767670489 1105 3 0.3583291780459277 1105 4 0.3426643004325953 1105 5 0.2856753781163377 1105 6 0.35574544724544666 1105 7 0.2715202134058927 1105 8 0.31373210550618325 1105 9 0.3753186750337534 1105 10-11 0.41675852177023387 1105 12-13 0.43094751178166035 1105 14-15 0.48548147220379434 1105 16-17 0.5099849224089539 1105 18-19 0.5721095679622863 1105 20-21 0.553922803035583 1105 22-23 0.5707094721020667 1105 24-25 0.5066507465689867 1105 26-27 0.4984275009377086 1105 28-29 0.5639481592056441 1105 30-31 0.6929069354262296 1105 32-33 0.8029504723235874 1105 34-35 0.9175432057468598 1105 36-37 0.9914730364444821 1105 38-39 1.009015319687819 1105 40-41 1.0928464011590293 1105 42-43 1.2157488140638648 1105 44-45 1.3617837206156835 1105 46-47 1.4553894920512818 1105 48-49 1.4909371734861168 1105 50-51 1.7661672764022214 1105 52-53 1.7091650527781397 1105 54-55 1.9229458988537047 1105 56-57 2.1436645708185296 1105 58-59 2.263240824137867 1105 60-61 2.304573370002025 1105 62-63 2.500812565512607 1105 64-65 2.4395164766595663 1105 66-67 2.5433783156902336 1105 68-69 2.784140757779422 1105 70-71 3.0933778317413427 1105 72-73 3.190132412274192 1105 74-75 3.3314719144265297 1105 76-77 3.447625654656317 1105 78-79 3.7037682394445106 1105 80-81 3.9879714797295236 1105 82-83 4.191185097393639 1105 84-85 4.072964316340975 1105 86-87 4.308516809154838 1105 88-89 4.505835420910582 1105 90-91 4.432560226827356 1105 92-93 4.639402234976526 1105 94-95 4.589658280139943 1105 96-97 4.407972984193826 1105 98-99 4.509113945433013 1105 100-101 3.749871498017498 1106 1 0.3686135670378796 1106 2 0.2712644434923277 1106 3 0.382113712473064 1106 4 0.28659151313525655 1106 5 0.3062120008024536 1106 6 0.33165914593503487 1106 7 0.317945702300392 1106 8 0.21928188378186775 1106 9 0.26523966113239084 1106 10-11 0.26994049483543137 1106 12-13 0.33673617209742446 1106 14-15 0.6070629878370042 1106 16-17 0.6522372686364264 1106 18-19 0.6347163516496792 1106 20-21 0.578723942275893 1106 22-23 0.5754961538557382 1106 24-25 0.6666364176647832 1106 26-27 0.8156162390458448 1106 28-29 0.8318138837917246 1106 30-31 0.9330840416465662 1106 32-33 0.7521786964877606 1106 34-35 0.8195696246137558 1106 36-37 0.8678978904632473 1106 38-39 1.0156513783792676 1106 40-41 1.0952488834427854 1106 42-43 0.9022027759295241 1106 44-45 1.2392167412258388 1106 46-47 1.4086248670056705 1106 48-49 1.581680002203072 1106 50-51 1.802669617406032 1106 52-53 1.7330568731677758 1106 54-55 1.8523748401863642 1106 56-57 1.6498071944465167 1106 58-59 2.01231221792316 1106 60-61 2.3399423774582004 1106 62-63 2.1936446175756323 1106 64-65 2.4957831421475554 1106 66-67 2.6559375640616523 1106 68-69 2.580973089509225 1106 70-71 2.8961488634063706 1106 72-73 3.161040236354143 1106 74-75 3.407729330016185 1106 76-77 3.31316138530185 1106 78-79 3.6944665063141215 1106 80-81 3.6953120495746923 1106 82-83 3.6596574899429264 1106 84-85 3.926668052785576 1106 86-87 4.2531938181347115 1106 88-89 4.3473113703712585 1106 90-91 4.509438052627097 1106 92-93 4.486357403106087 1106 94-95 4.528001600041769 1106 96-97 4.499311109365603 1106 98-99 4.369227515861622 1106 100-101 3.7073854532290227 1107 1 0.435203114071701 1107 2 0.3671887812214294 1107 3 0.25265767227828917 1107 4 0.22446123531084083 1107 5 0.22748862402811199 1107 6 0.2256013530311165 1107 7 0.24456876650612003 1107 8 0.2072909379389003 1107 9 0.31517475793824445 1107 10-11 0.3068884947837702 1107 12-13 0.30632006512662713 1107 14-15 0.37733070544030767 1107 16-17 0.42360769473719984 1107 18-19 0.41112928772810875 1107 20-21 0.5523917315633113 1107 22-23 0.5192741655576825 1107 24-25 0.4848792201911678 1107 26-27 0.6256730858484687 1107 28-29 0.5111190482777346 1107 30-31 0.6177530030662979 1107 32-33 0.6652709784969844 1107 34-35 0.8659955897662286 1107 36-37 1.1152549419854054 1107 38-39 0.9180269644454242 1107 40-41 0.9567947903438423 1107 42-43 0.9452297995530046 1107 44-45 1.063612834763937 1107 46-47 1.4717327290105047 1107 48-49 1.3686162969733573 1107 50-51 1.2933159692653007 1107 52-53 1.5256600886205476 1107 54-55 1.5713779142082984 1107 56-57 1.7963067811326425 1107 58-59 1.831234728029969 1107 60-61 1.9428393686623622 1107 62-63 1.9846478562773093 1107 64-65 2.2196139966739956 1107 66-67 2.5226813772696133 1107 68-69 2.38354306225634 1107 70-71 2.6249080015177846 1107 72-73 2.8649545286187177 1107 74-75 2.8715659806238847 1107 76-77 3.0549500010878567 1107 78-79 3.3382674440199693 1107 80-81 3.442149229740796 1107 82-83 3.7484816281189204 1107 84-85 3.7042232527907437 1107 86-87 3.924254501006729 1107 88-89 4.03656714707844 1107 90-91 4.112703958753183 1107 92-93 4.418865750108914 1107 94-95 4.366283667386099 1107 96-97 4.4108966524212825 1107 98-99 4.572088342101891 1107 100-101 3.898733227792139 1108 1 0.39259687189617054 1108 2 0.40011123415523997 1108 3 0.342365280302829 1108 4 0.30874054017419894 1108 5 0.31740047832845875 1108 6 0.22487497822768887 1108 7 0.3057780494353963 1108 8 0.2629665965585204 1108 9 0.42225992906166 1108 10-11 0.39310430165462407 1108 12-13 0.3980442320235511 1108 14-15 0.5482641578249101 1108 16-17 0.624121039395753 1108 18-19 0.5216108526895056 1108 20-21 0.5051727855252608 1108 22-23 0.6085702962207193 1108 24-25 0.5573864349030444 1108 26-27 0.5467571645314706 1108 28-29 0.5735976216068366 1108 30-31 0.6355198706533116 1108 32-33 0.6586925641303267 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42-43 0.9342114653942275 2311 44-45 1.0777344562888445 2311 46-47 1.0767220364105867 2311 48-49 1.1196126447419346 2311 50-51 1.2737416764512446 2311 52-53 1.2858700364601319 2311 54-55 1.435700701899357 2311 56-57 1.6721611513571162 2311 58-59 1.7640189622422895 2311 60-61 1.918162741082611 2311 62-63 1.9396162830480357 2311 64-65 2.316023011523395 2311 66-67 2.3556094140681836 2311 68-69 2.6171398696492645 2311 70-71 3.2496912330304646 2311 72-73 3.4967410910122574 2311 74-75 3.465208177888755 2311 76-77 3.50185057851688 2311 78-79 3.920015018503161 2311 80-81 4.104307445865867 2311 82-83 4.304127073958828 2311 84-85 4.561060415372655 2311 86-87 4.475079688449291 2311 88-89 4.568274212782818 2311 90-91 4.872792416041474 2311 92-93 5.297082565832618 2311 94-95 5.310710954843842 2311 96-97 5.028222708011349 2311 98-99 4.760213776891346 2311 100-101 4.591450969635275 2312 1 -0.08520542417094745 2312 2 -0.07153394915248512 2312 3 -0.09059779274059565 2312 4 -1.22821422188224E-4 2312 5 -0.0914779451991592 2312 6 -0.006298978669597943 2312 7 -0.01497144249861293 2312 8 0.0739887131035033 2312 9 0.04315313082975791 2312 10-11 -0.15489381414165138 2312 12-13 -0.022532870258295645 2312 14-15 0.0612266495947793 2312 16-17 0.2097560697919647 2312 18-19 0.0970614402327783 2312 20-21 0.05434057726701269 2312 22-23 -0.001527851271411862 2312 24-25 0.07570066480879944 2312 26-27 0.04631088170277309 2312 28-29 0.16271837405997758 2312 30-31 0.20837510831194095 2312 32-33 0.22861392403260794 2312 34-35 0.3759504246646941 2312 36-37 0.5998097807666483 2312 38-39 0.5122503521192456 2312 40-41 0.7238549471528515 2312 42-43 0.8748985356512478 2312 44-45 0.9817736222480633 2312 46-47 0.985649737269739 2312 48-49 1.0645681130070415 2312 50-51 1.2366248919035954 2312 52-53 1.3111856581418024 2312 54-55 1.5817504950950152 2312 56-57 1.6919891943385466 2312 58-59 1.8833780942930147 2312 60-61 2.107916116053964 2312 62-63 2.179382835448944 2312 64-65 2.3293669614571364 2312 66-67 2.5567089547450266 2312 68-69 2.758101395543452 2312 70-71 3.0781767858734916 2312 72-73 3.2186760040212796 2312 74-75 2.8564945373929653 2312 76-77 3.3133479088218074 2312 78-79 3.755987084806957 2312 80-81 4.1106440406769345 2312 82-83 4.5474343510600725 2312 84-85 4.828124112827464 2312 86-87 4.934414048773295 2312 88-89 5.049010084016476 2312 90-91 4.958185611210709 2312 92-93 5.171990178091587 2312 94-95 5.186522195145237 2312 96-97 5.032460041648221 2312 98-99 4.940886460892903 2312 100-101 4.492560190123584 2313 1 -0.3429378928470044 2313 2 -0.19633259623807575 2313 3 -0.21616095340331398 2313 4 -0.07341675873207265 2313 5 -0.10407691633928806 2313 6 -0.09009693093076265 2313 7 -0.13888243882605167 2313 8 -0.10743280968115698 2313 9 -0.08283731880403877 2313 10-11 -0.10927348180118912 2313 12-13 -0.13289264934369527 2313 14-15 -0.0671882293926771 2313 16-17 0.006301038338840215 2313 18-19 -0.07974435766810473 2313 20-21 -0.12703377246089786 2313 22-23 0.03939311444300131 2313 24-25 -0.005153437711619802 2313 26-27 0.06308448380785592 2313 28-29 0.2383435019587523 2313 30-31 0.19088335363073128 2313 32-33 0.22376993765979591 2313 34-35 0.4803542177623399 2313 36-37 0.5407954747066768 2313 38-39 0.5195478542062304 2313 40-41 0.7173849298752586 2313 42-43 0.6913568829494778 2313 44-45 0.7625796953620139 2313 46-47 0.9125723973753921 2313 48-49 1.2008833347410786 2313 50-51 1.347562633213343 2313 52-53 1.4460425015718457 2313 54-55 1.6957713906182832 2313 56-57 1.878151129476258 2313 58-59 1.9434073223941652 2313 60-61 2.0546748619682234 2313 62-63 2.18960108059942 2313 64-65 2.428631549005164 2313 66-67 2.5150484277119887 2313 68-69 2.7399943240733897 2313 70-71 3.069673971583555 2313 72-73 3.4681962267194457 2313 74-75 3.5252754527244896 2313 76-77 3.661532831338402 2313 78-79 4.082010710609303 2313 80-81 4.0256035692948124 2313 82-83 4.299432451144913 2313 84-85 4.4605823650413505 2313 86-87 4.540476355005719 2313 88-89 4.592853638375633 2313 90-91 4.5843544916397825 2313 92-93 4.608183340464052 2313 94-95 4.766755075016796 2313 96-97 4.779350089594946 2313 98-99 4.567166150020244 2313 100-101 4.056580819883155 2314 1 -0.24965241998339494 2314 2 -0.22758093268438273 2314 3 -0.18052328108073468 2314 4 -0.2102963305373109 2314 5 -0.2397062151418723 2314 6 -0.2967519514027259 2314 7 -0.25844493823257153 2314 8 -0.26363953030859477 2314 9 -0.2062248766741206 2314 10-11 -0.4291392119764481 2314 12-13 -0.3689256323799768 2314 14-15 -0.24443262769484164 2314 16-17 -0.2575182910256615 2314 18-19 -0.3267025542363342 2314 20-21 -0.2603753162559812 2314 22-23 -0.25983793169163505 2314 24-25 -0.25673368782951655 2314 26-27 -0.2269109438473791 2314 28-29 -0.1829507334626186 2314 30-31 -0.08564588563641706 2314 32-33 -0.052787501090499234 2314 34-35 0.07651136197190311 2314 36-37 0.1969752907251916 2314 38-39 0.18663619426667566 2314 40-41 0.23885387260470026 2314 42-43 0.3219650191909693 2314 44-45 0.5121036852090484 2314 46-47 0.578166740328868 2314 48-49 0.5770309889613117 2314 50-51 0.5439628972525838 2314 52-53 0.8699553323654428 2314 54-55 1.070981987523652 2314 56-57 1.4653833530831974 2314 58-59 1.583958582004641 2314 60-61 1.683591676658196 2314 62-63 1.9642135463248245 2314 64-65 2.070433585180492 2314 66-67 2.1524630703223835 2314 68-69 2.38916441040697 2314 70-71 2.7375293807479864 2314 72-73 2.9867035043442236 2314 74-75 3.2181579031790015 2314 76-77 3.4126880030228968 2314 78-79 3.576043714363575 2314 80-81 3.764226804345274 2314 82-83 3.9093307287032175 2314 84-85 4.120533574180513 2314 86-87 4.367439866313283 2314 88-89 4.500325666934227 2314 90-91 4.3996611135402475 2314 92-93 4.485514365851934 2314 94-95 4.50186862541517 2314 96-97 4.502414835320494 2314 98-99 4.7392955793173765 2314 100-101 4.263270730021759 2315 1 -0.5762465367803031 2315 2 -0.5083354689819366 2315 3 -0.30626039150856244 2315 4 -0.2001781280883108 2315 5 -0.1596404392410946 2315 6 -0.1374326690903942 2315 7 -0.23176140283857904 2315 8 -0.3020782187181581 2315 9 -0.4223158663024478 2315 10-11 -0.29957919450092874 2315 12-13 -0.21758382937891696 2315 14-15 -0.2994531978335502 2315 16-17 -0.2574362366375311 2315 18-19 -0.48608168798471496 2315 20-21 -0.45641124807940514 2315 22-23 -0.2746598502252837 2315 24-25 -0.19339556086504217 2315 26-27 -0.25385401933962015 2315 28-29 -0.16930984649191316 2315 30-31 -0.21550902167513897 2315 32-33 -0.1560214369099029 2315 34-35 0.11741581922142785 2315 36-37 0.07875022505410101 2315 38-39 0.2265435872705055 2315 40-41 0.3486688461652605 2315 42-43 0.48174739164672076 2315 44-45 0.6141590717831349 2315 46-47 0.7993613749924009 2315 48-49 0.806480432977331 2315 50-51 0.9816386591310291 2315 52-53 1.0390611519108788 2315 54-55 1.1934938778250697 2315 56-57 1.3768372906457387 2315 58-59 1.7209487277553066 2315 60-61 1.8777468391617305 2315 62-63 1.8763872180918177 2315 64-65 2.261503502690328 2315 66-67 2.399094013680312 2315 68-69 2.5359889469582697 2315 70-71 2.8912736534895984 2315 72-73 3.273496967795129 2315 74-75 3.599904015042167 2315 76-77 3.9152181742145515 2315 78-79 4.124221500158317 2315 80-81 4.426306837575229 2315 82-83 4.466718602416137 2315 84-85 4.784390946829344 2315 86-87 4.845363098710063 2315 88-89 4.740960020444941 2315 90-91 4.832213923468636 2315 92-93 4.92379429625878 2315 94-95 5.042841760118964 2315 96-97 5.087448142506748 2315 98-99 4.9405758208413655 2315 100-101 4.690820152965657 2316 1 -0.6994132668975936 2316 2 -0.598020869240024 2316 3 -0.5151876474255417 2316 4 -0.3913832944525595 2316 5 -0.35260285834154814 2316 6 -0.28644069683068096 2316 7 -0.38681619286644775 2316 8 -0.45662540373523797 2316 9 -0.37662029087118754 2316 10-11 -0.42480291976136897 2316 12-13 -0.3990276937033457 2316 14-15 -0.3957947336024006 2316 16-17 -0.32577276722319937 2316 18-19 -0.3812633133425507 2316 20-21 -0.47112993931547464 2316 22-23 -0.37883630229698895 2316 24-25 -0.37816687491928036 2316 26-27 -0.25280502391960624 2316 28-29 -0.20197167443029684 2316 30-31 -0.3573366222031851 2316 32-33 -0.09417087879159425 2316 34-35 -0.10536015392165154 2316 36-37 0.14372778660533214 2316 38-39 -0.026255656595793653 2316 40-41 0.18332846239609069 2316 42-43 0.31681325669300975 2316 44-45 0.5545622469513631 2316 46-47 0.5146303974077711 2316 48-49 0.766480581303469 2316 50-51 0.7272504714951609 2316 52-53 0.8506167058183749 2316 54-55 1.2210184711230383 2316 56-57 1.3088316097545984 2316 58-59 1.6225600852526156 2316 60-61 1.804159151220155 2316 62-63 2.025286473329796 2316 64-65 2.267676136187969 2316 66-67 2.3066093695559147 2316 68-69 2.5221486760817804 2316 70-71 2.7740160719637856 2316 72-73 3.357037545706728 2316 74-75 3.572538872344179 2316 76-77 3.862791270160141 2316 78-79 4.12086829936263 2316 80-81 4.282543580926511 2316 82-83 4.324175534273461 2316 84-85 4.687579917627225 2316 86-87 5.090096490714622 2316 88-89 5.379329973854055 2316 90-91 5.579050662534939 2316 92-93 5.587894920711825 2316 94-95 5.558445257978946 2316 96-97 5.836013293115464 2316 98-99 5.6741468468132705 2316 100-101 5.397007083455083 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6423.0 3 2076.0 4 2208.0 5 2602.0 6 2959.0 7 3756.0 8 4883.0 9 6194.0 10 7996.0 11 10301.0 12 12830.0 13 16114.0 14 19024.0 15 23147.0 16 27621.0 17 33419.0 18 34432.0 19 32578.0 20 34894.0 21 38198.0 22 42947.0 23 47366.0 24 52991.0 25 58082.0 26 62900.0 27 69197.0 28 75311.0 29 82172.0 30 89808.0 31 100320.0 32 113484.0 33 130720.0 34 157421.0 35 195931.0 36 243572.0 37 275627.0 38 162083.0 39 11874.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.57771381650936 18.449820459085505 14.821798420460135 23.150667303944996 2 15.449693636029396 19.339550020694666 40.96990615654416 24.240850186731773 3 19.51658397291216 23.53343273451248 29.374575225724726 27.57540806685064 4 12.854975590520956 16.34776441275923 36.914146331250066 33.88311366546975 5 13.992662582179472 36.94912104121702 34.211441363264136 14.846775013339371 6 32.66938927937436 35.74827422681042 17.135663681565124 14.4466728122501 7 28.83159275762323 32.471536091503225 20.149010262011018 18.547860888862523 8 26.16607248312312 35.363981399016566 19.232748686966314 19.237197430893996 9 26.028989578162903 17.08829730717595 20.05852524070021 36.82418787396094 10-11 25.178290340547527 26.47614283116032 26.812900147377665 21.532666680914485 12-13 26.074636913613602 23.683436343291508 27.355964338731027 22.885962404363863 14-15 22.80902490429602 25.197224884451963 25.8233813188886 26.170368892363417 16-17 22.31317060468995 28.378606771290503 26.54718442257312 22.761038201446425 18-19 22.23960475408127 26.926716999927162 28.878457431381282 21.955220814610282 20-21 22.823369765533773 25.964562026268347 29.403542488797502 21.808525719400375 22-23 22.554954508600865 25.888075587990674 29.188093774955288 22.36887612845318 24-25 22.415550440495917 26.0101873200953 29.293332917459065 22.28092932194972 26-27 22.080031406247443 26.398390429818484 29.122228098777008 22.39935006515706 28-29 22.126036402510284 26.680112084891068 29.009609301414713 22.184242211183932 30-31 22.324211444617102 26.259153240097056 28.996374144652542 22.4202611706333 32-33 22.04010686440216 26.23824218962979 29.01154789815168 22.710103047816368 34-35 22.61551613965529 26.40232133627317 28.821274117885675 22.16088840618587 36-37 22.62304799440994 26.091535442515667 28.818542101208998 22.466874461865395 38-39 22.23738636883982 26.510048821332237 28.661261940947774 22.591302868880163 40-41 22.742700029653403 26.23778977480856 28.705607109839697 22.31390308569834 42-43 22.385882562258956 26.22784502111493 28.615070705802115 22.771201710823995 44-45 22.421941073624065 26.58908104933105 28.561128162349398 22.427849714695487 46-47 22.651856723150633 26.739251118246532 28.06836948600998 22.540522672592854 48-49 22.543653447348436 26.716958554545034 28.104920466267636 22.634467531838894 50-51 22.69956694703986 26.93108871982289 27.931850519763845 22.437493813373404 52-53 22.83968947062276 26.78690872051397 27.86917877328338 22.504223035579894 54-55 22.640417274035794 26.89212470256536 27.8855792873429 22.581878736055945 56-57 22.61953558465298 27.030748810007623 27.79574079532829 22.55397481001111 58-59 22.661691227736647 26.718769825922134 27.799828987454866 22.819709958886353 60-61 22.560200163212553 26.9170770781421 27.915211449210176 22.60751130943517 62-63 22.514268496720284 26.878123993007573 28.03220544367841 22.575402066593732 64-65 22.347866526286136 26.888067604169642 27.88517198322672 22.8788938863175 66-67 22.356243790525777 26.88597415899437 27.810661208034198 22.94712084244565 68-69 22.604527385192714 26.6531069386374 27.98631061951707 22.756055056652816 70-71 22.676602784459206 26.607265393645186 27.68674539592163 23.029386425973982 72-73 22.63214758010621 26.74766230835398 27.573281468213175 23.046908643326635 74-75 22.48897947472136 27.01071985648134 27.5199381132477 22.980362555549597 76-77 22.74276265556873 26.683473750904273 27.547956441409877 23.02580715211712 78-79 22.731640144410378 26.73955354353318 27.542252506240395 22.98655380581605 80-81 22.789961653278308 26.71897443232113 27.344888513687348 23.14617540071321 82-83 22.824555038478444 26.731878850460834 27.39179899809526 23.051767112965457 84-85 22.907625657357002 26.639819706331668 27.364037936320333 23.088516699990997 86-87 22.99979376058176 26.619731574818907 27.18974593309384 23.19072873150549 88-89 23.032734782082496 26.665038450257804 27.08605596194335 23.21617080571635 90-91 22.952339625686058 26.650472417078973 27.06384008687328 23.33334787036169 92-93 22.95785095423409 26.364914083173 27.187887567658088 23.489347394934825 94-95 22.91396672604628 26.29888814237325 27.2018376932536 23.58530743832687 96-97 23.09696068005337 26.06020113855385 27.16615572679679 23.676682454595984 98-99 22.97366778788224 26.07351702778007 26.896613981309585 24.05620120302811 100-101 23.778226360004044 25.082635961118704 27.007778311042085 24.13135936783517 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 664.0 1 445.0 2 334.0 3 435.0 4 342.0 5 337.5 6 663.5 7 1489.0 8 2564.5 9 3065.5 10 2649.0 11 2106.5 12 2060.0 13 2235.0 14 2389.5 15 2509.5 16 2708.0 17 2933.5 18 3141.0 19 3473.0 20 4049.0 21 4765.0 22 5791.0 23 7235.5 24 9025.0 25 11231.0 26 14087.5 27 17273.5 28 20117.5 29 23029.0 30 26301.5 31 30185.0 32 34658.0 33 38001.0 34 40589.5 35 44017.0 36 48434.0 37 52855.5 38 56338.0 39 59209.5 40 62621.5 41 66541.5 42 68985.5 43 71881.5 44 75766.5 45 78400.5 46 80443.0 47 83367.5 48 85282.0 49 85118.5 50 84407.5 51 82856.5 52 80994.0 53 79938.5 54 79282.5 55 75791.5 56 70072.0 57 64360.5 58 59420.0 59 53753.5 60 45386.5 61 37710.0 62 31385.5 63 24836.5 64 19167.5 65 15008.5 66 11548.5 67 8869.0 68 6901.0 69 5301.5 70 4131.0 71 3048.5 72 2139.0 73 1610.0 74 1200.0 75 823.0 76 573.0 77 394.0 78 251.0 79 153.0 80 106.5 81 70.5 82 45.5 83 26.0 84 15.0 85 15.0 86 14.0 87 10.0 88 6.5 89 5.5 90 5.0 91 2.5 92 2.0 93 2.0 94 1.5 95 1.5 96 1.0 97 2.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3813014478990487 2 0.026161334332696306 3 0.0465235728883116 4 0.0396344215140349 5 0.059909455621874545 6 0.05668289105417532 7 0.03196043010977732 8 0.02960591001983465 9 0.033791723513066065 10-11 0.03104478340813295 12-13 0.0314590045350673 14-15 0.025441897638547158 16-17 0.03084857340063773 18-19 0.026597356571574575 20-21 0.03889318370794184 22-23 0.026597356571574575 24-25 0.048027849612441637 26-27 0.040266653760408395 28-29 0.03202583344560906 30-31 0.039176598163212714 32-33 0.03645145917022352 34-35 0.035579414692466976 36-37 0.035710221364130455 38-39 0.017702502898457834 40-41 0.013124269390235982 42-43 0.01294986049468467 44-45 0.009134665904499793 46-47 0.015784005047393437 48-49 0.013712899412721646 50-51 0.009069262568668052 52-53 0.0037933934782409646 54-55 0.004360222388782717 56-57 0.007368775837042793 58-59 0.0027033378810452846 60-61 0.005777294665137101 62-63 0.005319471314314915 64-65 0.007434179172874533 66-67 0.003946001261848359 68-69 0.006562134695117991 70-71 0.01798591735372871 72-73 0.014541341666590362 74-75 0.010464533733078523 76-77 0.007935604747584545 78-79 0.005908101336800583 80-81 0.007630389180369756 82-83 0.009984909270312424 84-85 0.003989603485736186 86-87 4.3602223887827176E-4 88-89 0.024569853160790613 90-91 0.020318636331727463 92-93 0.013647496076889904 94-95 0.025572704310210637 96-97 0.020253232995895723 98-99 0.01805132068956045 100-101 0.11072784756313711 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2293461.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.80396531840434 #Duplication Level Percentage of deduplicated Percentage of total 1 75.58648758674416 42.93612219417415 2 13.76327192228678 15.636168418826928 3 4.682044614507392 7.978760997050991 4 2.0385669809417872 4.631947523386461 5 1.0820100988680645 3.073123206513239 6 0.6598555161508 2.24894459127527 7 0.449077776558034 1.785657891140809 8 0.29849452800979337 1.356453825344142 9 0.22800766557096985 1.1656565574681337 >10 1.1162910862738942 11.097453005117961 >50 0.06215062530641678 2.402546748892646 >100 0.030348044628370808 3.2242118827055672 >500 0.0022366606921745344 0.8284176112026609 >1k 0.0010026409999400348 0.9008806039006062 >5k 1.5425246152923612E-4 0.733654943000513 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9158 0.39930916636472125 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7588 0.3308536748608326 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4592 0.20022141209290237 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.720444777565435E-5 2 4.3602223887827176E-5 0.0 0.0 0.0 8.720444777565435E-5 3 4.3602223887827176E-5 0.0 0.0 0.0 8.720444777565435E-5 4 4.3602223887827176E-5 0.0 0.0 0.0 8.720444777565435E-5 5 8.720444777565435E-5 0.0 0.0 0.0 1.3080667166348153E-4 6 8.720444777565435E-5 0.0 0.0 0.0 1.744088955513087E-4 7 8.720444777565435E-5 0.0 0.0 0.0 1.744088955513087E-4 8 8.720444777565435E-5 0.0 0.0 0.0 1.744088955513087E-4 9 8.720444777565435E-5 0.0 0.0 0.0 1.744088955513087E-4 10-11 8.720444777565435E-5 0.0 0.0 0.0 1.744088955513087E-4 12-13 8.720444777565435E-5 0.0 0.0 0.0 7.630389180369756E-4 14-15 8.720444777565435E-5 0.0 0.0 0.0 9.156467016443706E-4 16-17 8.720444777565435E-5 0.0 0.0 4.3602223887827176E-5 9.592489255321979E-4 18-19 1.0900555971956794E-4 0.0 0.0 1.0900555971956794E-4 0.0010464533733078522 20-21 1.3080667166348153E-4 0.0 0.0 1.3080667166348153E-4 0.0010900555971956793 22-23 2.1801111943913588E-4 0.0 0.0 1.744088955513087E-4 0.001111856709139593 24-25 2.6161334332696305E-4 0.0 0.0 2.6161334332696305E-4 0.0011336578210835065 26-27 2.6161334332696305E-4 0.0 0.0 5.450277985978397E-4 0.001220862268859161 28-29 2.6161334332696305E-4 0.0 0.0 0.002834144552708766 0.001220862268859161 30-31 2.6161334332696305E-4 0.0 0.0 0.010551738180854177 0.0012426633808030746 32-33 2.6161334332696305E-4 0.0 0.0 0.02509307984744454 0.0013080667166348152 34-35 3.0521556721479023E-4 0.0 0.0 0.04872548519464687 0.0013516689405226425 36-37 3.924200149904446E-4 2.1801111943913588E-5 0.0 0.0880764922534109 0.0013516689405226425 38-39 3.924200149904446E-4 4.3602223887827176E-5 0.0 0.1434513165909514 0.0013516689405226425 40-41 3.924200149904446E-4 4.3602223887827176E-5 0.0 0.2037749933397603 0.0013516689405226425 42-43 4.3602223887827176E-4 4.3602223887827176E-5 0.0 0.26436028343189616 0.0013516689405226425 44-45 5.232266866539261E-4 4.3602223887827176E-5 0.0 0.33715419621262366 0.001417072276354383 46-47 6.32232246373494E-4 4.3602223887827176E-5 0.0 0.4264951529587815 0.0015260778360739512 48-49 7.194366941491484E-4 4.3602223887827176E-5 0.0 0.5182778342426577 0.0015696800599617783 50-51 7.412378060930619E-4 4.3602223887827176E-5 0.0 0.6163174346544371 0.0016132822838496054 52-53 7.412378060930619E-4 4.3602223887827176E-5 0.0 0.7105636415879755 0.001744088955513087 54-55 7.412378060930619E-4 4.3602223887827176E-5 0.0 0.8112411765449685 0.001744088955513087 56-57 7.412378060930619E-4 4.3602223887827176E-5 0.0 0.9277245176612988 0.0018094922913448278 58-59 7.412378060930619E-4 4.3602223887827176E-5 0.0 1.0558932547795667 0.0018748956271765686 60-61 7.412378060930619E-4 4.3602223887827176E-5 0.0 1.1862857053161138 0.0018748956271765686 62-63 7.412378060930619E-4 4.3602223887827176E-5 0.0 1.312644950143037 0.0018748956271765686 64-65 7.412378060930619E-4 4.3602223887827176E-5 0.0 1.4490981097999924 0.0019839011868961363 66-67 7.412378060930619E-4 4.3602223887827176E-5 0.0 1.5995911855488276 0.00200570229884005 68-69 7.412378060930619E-4 4.3602223887827176E-5 0.0 1.7639715696059364 0.00200570229884005 70-71 7.630389180369756E-4 4.3602223887827176E-5 0.0 1.9393833163066647 0.0020275034107839636 72-73 7.848400299808892E-4 4.3602223887827176E-5 0.0 2.114184631872964 0.0022019123063352726 74-75 7.848400299808892E-4 4.3602223887827176E-5 0.0 2.2974447788735013 0.002332718977998754 76-77 7.848400299808892E-4 4.3602223887827176E-5 0.0 2.4922813163162574 0.0023545200899426676 78-79 7.848400299808892E-4 4.3602223887827176E-5 0.0 2.7055833955755078 0.002376321201886581 80-81 8.284422538687163E-4 4.3602223887827176E-5 0.0 2.92597083621653 0.0024635256496622353 82-83 8.284422538687163E-4 4.3602223887827176E-5 0.0 3.1530294171123905 0.002594332321325717 84-85 8.284422538687163E-4 4.3602223887827176E-5 0.0 3.378561920172176 0.002768741216877026 86-87 8.502433658126298E-4 4.3602223887827176E-5 0.0 3.611572204628725 0.002834144552708766 88-89 8.720444777565435E-4 4.3602223887827176E-5 0.0 3.86110773193876 0.0028777467765965935 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4465 0.0 53.82979 1 GTATCAA 8025 0.0 41.89457 1 ATCAACG 10100 0.0 32.60881 3 TATCAAC 10200 0.0 32.568676 2 TCAACGC 10250 0.0 32.270706 4 CAACGCA 10435 0.0 31.516409 5 AACGCAG 10875 0.0 30.323372 6 GTACGCT 95 3.4797693E-5 30.015858 3 ACGCAGA 12135 0.0 26.817747 7 CGCAGAG 12190 0.0 26.618801 8 TGGTATC 2140 0.0 23.762554 2 GCAGAGT 13350 0.0 23.558529 9 GTGGTAT 2345 0.0 23.18332 1 GAGTACT 8130 0.0 22.935156 12-13 GTACATG 7840 0.0 22.384394 1 GGACCGA 895 0.0 22.298391 6 TACATGG 7600 0.0 22.199228 2 CAGAGTA 12445 0.0 21.682568 10-11 GTACTTT 8735 0.0 21.237856 14-15 AGAGTAC 11700 0.0 21.073603 10-11 >>END_MODULE