FastQCFastQC Report
Thu 26 May 2016
SRR938235_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938235_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293461
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86300.37628719215194856No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75460.32902238145754387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44900.19577398525634399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46700.054.3879621
GTATCAA82700.040.5473061
ATCAACG102000.032.7287943
TCAACGC103150.032.2241944
TATCAAC104400.032.164932
CAACGCA104350.031.7624845
AACGCAG108550.030.5837616
ACGCAGA123400.026.8263267
CGCAGAG124800.026.525398
TGGTATC20750.023.6100852
GCAGAGT139850.023.4331259
GAGTACT85000.022.90912412-13
TACATGG77600.022.4948312
GTACATG81200.022.0247121
GTACTTT87700.021.87889514-15
CAGAGTA130300.021.79718210-11
GTGGTAT24350.021.6821541
ACATGGG79250.021.122073
AGAGTAC122450.020.78976410-11
AGTACTT87150.019.97331612-13
Produced by FastQC (version 0.11.5)