##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938235_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2293461 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46790636509625 33.0 31.0 34.0 31.0 34.0 2 32.69544195432144 34.0 31.0 34.0 31.0 34.0 3 32.76492427819789 34.0 31.0 34.0 31.0 34.0 4 36.22754779784788 37.0 35.0 37.0 35.0 37.0 5 36.15118417099746 37.0 35.0 37.0 35.0 37.0 6 36.20049087383653 37.0 37.0 37.0 35.0 37.0 7 36.17296740602958 37.0 37.0 37.0 35.0 37.0 8 36.166540002206276 37.0 37.0 37.0 35.0 37.0 9 37.85763089060595 39.0 38.0 39.0 35.0 39.0 10-11 37.82474042506064 39.0 38.0 39.0 35.0 39.0 12-13 37.7443403223338 39.0 38.0 39.0 35.0 39.0 14-15 38.99151435319807 40.0 38.0 41.0 35.5 41.0 16-17 38.90160416941906 40.0 38.0 41.0 35.0 41.0 18-19 39.14939146556231 40.0 38.5 41.0 36.0 41.0 20-21 39.11815505038019 40.0 39.0 41.0 36.0 41.0 22-23 39.011125107424974 40.0 38.5 41.0 35.0 41.0 24-25 38.91089667537403 40.0 38.0 41.0 35.0 41.0 26-27 38.751128534559776 40.0 38.0 41.0 35.0 41.0 28-29 38.62516737803695 40.0 38.0 41.0 34.5 41.0 30-31 38.3878897439285 40.0 38.0 41.0 34.0 41.0 32-33 38.165135356563724 40.0 38.0 41.0 34.0 41.0 34-35 38.08441085329116 40.0 38.0 41.0 34.0 41.0 36-37 38.03099703897298 40.0 38.0 41.0 33.0 41.0 38-39 37.83702033738529 40.0 37.0 41.0 33.0 41.0 40-41 37.62171320986056 40.0 37.0 41.0 33.0 41.0 42-43 37.36570536843661 40.0 37.0 41.0 32.0 41.0 44-45 37.093806914527875 40.0 36.5 41.0 31.0 41.0 46-47 36.734914829595965 39.5 36.0 41.0 30.5 41.0 48-49 36.32776947155413 39.0 35.5 41.0 30.0 41.0 50-51 35.94922760840494 39.0 35.0 40.0 29.0 41.0 52-53 35.41832453222444 38.0 35.0 40.0 27.5 41.0 54-55 35.03363497351819 38.0 34.0 40.0 26.5 41.0 56-57 34.652559385138886 38.0 34.0 40.0 26.0 41.0 58-59 34.138007142916315 37.5 33.5 40.0 24.5 41.0 60-61 33.65203332430767 37.0 33.0 40.0 23.0 41.0 62-63 33.0872330072323 36.0 32.0 39.0 21.5 40.5 64-65 32.507291381889644 36.0 31.0 39.0 19.5 40.0 66-67 31.932955476461125 35.0 31.0 38.5 17.0 40.0 68-69 31.300398393519664 35.0 30.0 38.0 10.5 40.0 70-71 30.67312502806893 34.5 29.5 37.5 7.5 39.5 72-73 29.98917836405328 34.0 29.0 36.5 4.5 39.0 74-75 29.315326704923258 34.0 28.0 36.0 2.0 39.0 76-77 28.037683440006173 32.0 26.5 34.5 2.0 37.0 78-79 28.685635596157944 33.5 28.0 35.0 2.0 37.0 80-81 28.40145090760209 34.0 27.5 35.0 2.0 37.0 82-83 27.82992538351426 33.0 26.0 35.0 2.0 36.5 84-85 27.158644947526902 33.0 25.5 35.0 2.0 36.0 86-87 26.428631879940404 32.5 24.5 35.0 2.0 35.5 88-89 25.61831398048626 32.0 21.5 34.5 2.0 35.0 90-91 24.954184527227625 31.5 19.0 34.0 2.0 35.0 92-93 23.99807125562632 31.0 9.5 34.0 2.0 35.0 94-95 23.07672290917526 30.5 2.0 34.0 2.0 35.0 96-97 22.021473223220276 30.0 2.0 34.0 2.0 35.0 98-99 20.60180574250009 29.0 2.0 34.0 2.0 35.0 100-101 16.766145794500105 21.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09839300189744904 1101 2 0.10301340724051755 1101 3 0.09523434109448914 1101 4 -0.013959495179456383 1101 5 -0.07291657861480161 1101 6 0.02026922245820373 1101 7 0.07838451008794323 1101 8 0.059589234806345814 1101 9 -0.07252042768858047 1101 10-11 0.10468555280581882 1101 12-13 0.1486547057798191 1101 14-15 0.36594979762015356 1101 16-17 0.3641446874021028 1101 18-19 0.3197376561367591 1101 20-21 0.2233901285043487 1101 22-23 0.2804252614555125 1101 24-25 0.27806347344807136 1101 26-27 0.31787516033350016 1101 28-29 0.37860998358429754 1101 30-31 0.4873973966496621 1101 32-33 0.4132436116766556 1101 34-35 0.4054578273421541 1101 36-37 0.4645210209013584 1101 38-39 0.5158956557692917 1101 40-41 0.6655099277244787 1101 42-43 0.664372985405457 1101 44-45 0.6024282994241972 1101 46-47 0.6350115165304047 1101 48-49 0.7768817261553096 1101 50-51 0.8210318579185625 1101 52-53 0.7610783514575914 1101 54-55 0.9909793040667196 1101 56-57 1.0606962517945817 1101 58-59 1.09567446743241 1101 60-61 1.034097794919191 1101 62-63 1.2244909458752105 1101 64-65 1.3716764048243917 1101 66-67 1.445707372138596 1101 68-69 1.7857333825995028 1101 70-71 1.8593357547599716 1101 72-73 1.9361880067907578 1101 74-75 2.0819307315841975 1101 76-77 2.110480139950525 1101 78-79 2.1812011584455178 1101 80-81 2.3506651804955148 1101 82-83 2.477160643976262 1101 84-85 2.485243206509775 1101 86-87 2.7917941738808487 1101 88-89 3.05484196397963 1101 90-91 3.261494215155647 1101 92-93 3.2577454610410648 1101 94-95 3.5576666196882023 1101 96-97 3.749380157934425 1101 98-99 3.9898111926629873 1101 100-101 4.068570142042621 1102 1 0.20340375165520186 1102 2 0.139388467802533 1102 3 0.1340773037882883 1102 4 0.15781709154534695 1102 5 -0.005707881909373214 1102 6 0.10380316746789475 1102 7 0.0991648516577115 1102 8 0.15860055990324184 1102 9 0.2444800870885544 1102 10-11 0.2693877390945829 1102 12-13 0.2892172512013289 1102 14-15 0.4358118676298446 1102 16-17 0.4211032629835003 1102 18-19 0.3361367143974192 1102 20-21 0.23376326622236832 1102 22-23 0.35006590159601814 1102 24-25 0.4259615929974885 1102 26-27 0.3320182835166392 1102 28-29 0.24934036149127792 1102 30-31 0.42632060401400196 1102 32-33 0.37721678270379044 1102 34-35 0.4183386110146401 1102 36-37 0.3479107526116323 1102 38-39 0.3686372225086316 1102 40-41 0.5866998199240925 1102 42-43 0.6304544715973179 1102 44-45 0.5655441394193517 1102 46-47 0.5895105172571462 1102 48-49 0.6573852993142282 1102 50-51 0.7001425047208869 1102 52-53 0.7845139588946068 1102 54-55 0.9610294948370708 1102 56-57 1.0128579372354736 1102 58-59 1.1501727111339548 1102 60-61 0.9806234277922528 1102 62-63 1.3050403190583566 1102 64-65 1.2701139048243917 1102 66-67 1.3375436124486733 1102 68-69 1.6201019478853596 1102 70-71 1.6445128229528017 1102 72-73 1.9350070474884333 1102 74-75 2.0789385895057073 1102 76-77 2.154680948453432 1102 78-79 2.1497619021470697 1102 80-81 2.0529063535817578 1102 82-83 2.207103261466571 1102 84-85 2.4425091343198524 1102 86-87 2.457696245101779 1102 88-89 2.731467297070715 1102 90-91 2.801669542190531 1102 92-93 2.9628746852416477 1102 94-95 3.140856118234712 1102 96-97 3.3787273751098112 1102 98-99 3.699486353152327 1102 100-101 3.5728246236317673 1103 1 0.21169182910934836 1103 2 0.1855317971131214 1103 3 0.15150013775765103 1103 4 0.015661987551958134 1103 5 -0.05388296915944579 1103 6 0.08132811935804085 1103 7 0.06699036781935774 1103 8 0.007365740328786785 1103 9 0.09190525859882115 1103 10-11 0.14670353543496617 1103 12-13 0.18802823112022793 1103 14-15 0.17216103644378933 1103 16-17 0.2553376367943443 1103 18-19 0.1942010946229047 1103 20-21 0.19777430974411914 1103 22-23 0.242518634344421 1103 24-25 0.3213464446451866 1103 26-27 0.36465280084162544 1103 28-29 0.341313422691357 1103 30-31 0.3424789914369484 1103 32-33 0.3140236746441687 1103 34-35 0.4477620674222109 1103 36-37 0.3527659066175204 1103 38-39 0.3472225617782314 1103 40-41 0.3875637587348777 1103 42-43 0.5508207724116545 1103 44-45 0.6156140617912556 1103 46-47 0.7550974302897586 1103 48-49 0.8262828226080927 1103 50-51 0.9608921350632542 1103 52-53 1.0179965822678412 1103 54-55 1.0707831560784697 1103 56-57 1.1738716961099698 1103 58-59 1.2415393027818737 1103 60-61 1.2085581288588259 1103 62-63 1.3130868279681849 1103 64-65 1.2024420955677257 1103 66-67 1.4339247050044257 1103 68-69 1.5724310627727789 1103 70-71 1.4459444988065435 1103 72-73 1.6467474825972097 1103 74-75 1.7924848429168883 1103 76-77 1.619172071234125 1103 78-79 1.6945237719623485 1103 80-81 1.8459096315751644 1103 82-83 2.013925551278156 1103 84-85 2.2549812740939252 1103 86-87 2.6226260645131525 1103 88-89 2.881512620901674 1103 90-91 3.0158885181301684 1103 92-93 3.211979080905781 1103 94-95 3.2854741341517375 1103 96-97 3.451621617692197 1103 98-99 3.949372533936657 1103 100-101 3.7844740033680075 1104 1 0.1699416827020528 1104 2 0.20330922297976173 1104 3 0.17267018893692665 1104 4 0.08535207570786696 1104 5 -0.03406828066246703 1104 6 0.09786029899973414 1104 7 0.0774486188628174 1104 8 0.09581694694771414 1104 9 0.09972807018566954 1104 10-11 0.14178701950237382 1104 12-13 0.19374909477073743 1104 14-15 0.25350389041573607 1104 16-17 0.28393189529197116 1104 18-19 0.2521556673656491 1104 20-21 0.22455705516590285 1104 22-23 0.277126030232985 1104 24-25 0.2977902844947522 1104 26-27 0.19606028603485015 1104 28-29 0.2815683217379288 1104 30-31 0.2931278888986171 1104 32-33 0.3627574543710139 1104 34-35 0.34905196597878074 1104 36-37 0.37391637913394504 1104 38-39 0.4080931654219384 1104 40-41 0.3566529441954387 1104 42-43 0.4690530290050603 1104 44-45 0.4959507888315855 1104 46-47 0.6388208333252905 1104 48-49 0.6289932883660896 1104 50-51 0.7840765818600275 1104 52-53 0.8478128954693318 1104 54-55 0.9390428768845709 1104 56-57 0.8675586482184059 1104 58-59 1.114494873928244 1104 60-61 1.1158760921846351 1104 62-63 1.0523394941818438 1104 64-65 1.3148848442043075 1104 66-67 1.4979473651973656 1104 68-69 1.6005745793472457 1104 70-71 1.641017209200914 1104 72-73 1.7131593886741427 1104 74-75 1.8326866524180154 1104 76-77 1.8092175002221005 1104 78-79 2.0013758280140053 1104 80-81 2.0682615849740564 1104 82-83 2.035654297449767 1104 84-85 2.4463447473184274 1104 86-87 2.610965618669141 1104 88-89 2.6983860317578596 1104 90-91 2.99343752482131 1104 92-93 2.9754455772705306 1104 94-95 3.455542404191906 1104 96-97 3.679068674716298 1104 98-99 4.10726136131321 1104 100-101 3.704915620119902 1105 1 0.1388486552938204 1105 2 0.21168986342896545 1105 3 0.22202397711731692 1105 4 0.13369378229368323 1105 5 0.02695158107633233 1105 6 0.062038907580181046 1105 7 0.13444024671507293 1105 8 0.127931998626849 1105 9 0.13617937157842874 1105 10-11 0.08699945513907892 1105 12-13 0.21550574196771066 1105 14-15 0.33484886109933854 1105 16-17 0.26092806363307375 1105 18-19 0.25261209835083775 1105 20-21 0.29479481355637915 1105 22-23 0.32144809920450257 1105 24-25 0.29858942710463054 1105 26-27 0.3253897519432769 1105 28-29 0.33334014808743007 1105 30-31 0.37527718066935734 1105 32-33 0.40258885143717293 1105 34-35 0.4032192523365623 1105 36-37 0.42775545360335343 1105 38-39 0.46214952879693527 1105 40-41 0.5000131835156552 1105 42-43 0.4737269154356696 1105 44-45 0.6033801637231022 1105 46-47 0.6237084831541679 1105 48-49 0.7663301300832472 1105 50-51 0.825426826304863 1105 52-53 0.9266732327663405 1105 54-55 1.0276049877478997 1105 56-57 0.8506940049139899 1105 58-59 1.0594752102144156 1105 60-61 0.971485232321271 1105 62-63 1.2315753953632367 1105 64-65 1.283095104466291 1105 66-67 1.3564756129622246 1105 68-69 1.6016026106517671 1105 70-71 1.7415710162723919 1105 72-73 1.7484816784559314 1105 74-75 1.6912443139336837 1105 76-77 1.5955368540144264 1105 78-79 1.8058750547637814 1105 80-81 1.8592315220493418 1105 82-83 1.9628614783820382 1105 84-85 2.249965082908613 1105 86-87 2.409701200234924 1105 88-89 2.3797124842068023 1105 90-91 2.6233582692626314 1105 92-93 2.849143449164945 1105 94-95 3.0310826125821144 1105 96-97 3.4036693830491274 1105 98-99 3.4843034938184267 1105 100-101 3.3601243189674186 1106 1 0.11942065422015702 1106 2 0.11720950519832485 1106 3 0.05234098101819029 1106 4 0.09427764294891006 1106 5 -0.02148662462018791 1106 6 0.0012648252970706153 1106 7 0.044169757507155794 1106 8 0.034473870690547415 1106 9 0.12227398574275128 1106 10-11 0.12853419087808504 1106 12-13 0.1743446989070634 1106 14-15 0.13062541055776222 1106 16-17 0.09622643111843843 1106 18-19 -0.03667565947701945 1106 20-21 0.05355810777387404 1106 22-23 0.04318588266848877 1106 24-25 0.021137681678546016 1106 26-27 -0.10973780182426651 1106 28-29 0.08686149839579116 1106 30-31 0.3324588306712073 1106 32-33 0.2540008070312325 1106 34-35 0.2337642891352445 1106 36-37 0.25017388010805064 1106 38-39 0.2430766161317237 1106 40-41 0.2986020717437796 1106 42-43 0.21634206501721565 1106 44-45 0.26369392091768873 1106 46-47 0.3704095402624503 1106 48-49 0.3628525379820644 1106 50-51 0.5307824049338237 1106 52-53 0.4342941524842985 1106 54-55 0.4884839852550087 1106 56-57 0.5248207293248797 1106 58-59 0.8054811185769353 1106 60-61 0.5840081159750667 1106 62-63 0.5703645748305561 1106 64-65 0.734207262095488 1106 66-67 0.8695460166628344 1106 68-69 0.9538945522303379 1106 70-71 0.916813996877341 1106 72-73 1.236716788209069 1106 74-75 1.4959892867626117 1106 76-77 1.667243054419778 1106 78-79 1.6494374550119453 1106 80-81 1.5666279448862248 1106 82-83 2.028279395378867 1106 84-85 2.120662795266508 1106 86-87 2.1897951136250136 1106 88-89 2.2877258214190377 1106 90-91 2.313669302117045 1106 92-93 2.1256653829160648 1106 94-95 2.1605773720445747 1106 96-97 2.561629880920286 1106 98-99 2.8568027665240727 1106 100-101 3.1512851611180217 1107 1 0.18165022424457078 1107 2 0.16275233484843454 1107 3 0.1801671760765018 1107 4 0.1047525664212614 1107 5 0.015620404298864798 1107 6 0.028162517197415582 1107 7 0.09088310496204599 1107 8 0.11802139532792921 1107 9 0.1258517339571057 1107 10-11 0.12981974232919669 1107 12-13 0.19938853278071633 1107 14-15 0.3304949583665575 1107 16-17 0.3341030956754878 1107 18-19 0.24951473291283577 1107 20-21 0.21058064952503486 1107 22-23 0.17886100504814095 1107 24-25 0.2654190967619172 1107 26-27 0.2309171876491476 1107 28-29 0.23430355372818212 1107 30-31 0.3018364703906684 1107 32-33 0.29883123532773226 1107 34-35 0.2727893527468339 1107 36-37 0.3748090014544161 1107 38-39 0.38061891622343325 1107 40-41 0.42953659701404945 1107 42-43 0.4473829055943028 1107 44-45 0.642707989734447 1107 46-47 0.7548160635178149 1107 48-49 0.9229937427920021 1107 50-51 0.8728880292315111 1107 52-53 0.89960444464554 1107 54-55 0.9925618271107837 1107 56-57 1.0239961197127485 1107 58-59 0.9764425092489617 1107 60-61 1.0808235002923965 1107 62-63 1.0825363454930184 1107 64-65 1.1435401638171996 1107 66-67 1.2938413649443525 1107 68-69 1.4338916138270221 1107 70-71 1.6152530912033178 1107 72-73 1.6431068467188155 1107 74-75 1.6921224610131524 1107 76-77 1.5680049404226466 1107 78-79 1.612497527600194 1107 80-81 1.7234995302032488 1107 82-83 1.9529327606579159 1107 84-85 2.015488541491788 1107 86-87 2.242621793035525 1107 88-89 2.4905890694202775 1107 90-91 2.54078406452615 1107 92-93 2.846213944067145 1107 94-95 2.8882576156414395 1107 96-97 3.1536963534155404 1107 98-99 3.3886263202484166 1107 100-101 3.1005367562512554 1108 1 0.2139475727100404 1108 2 0.11000029750248785 1108 3 0.13329990460207597 1108 4 0.09644115012044807 1108 5 -0.004108975781704771 1108 6 0.03749473847586415 1108 7 0.13022567073872438 1108 8 0.10137212583882871 1108 9 0.1581763721128624 1108 10-11 0.13389560681541468 1108 12-13 0.22436851323448082 1108 14-15 0.26394393176251896 1108 16-17 0.26176537552443335 1108 18-19 0.24118167674041757 1108 20-21 0.28298648730147136 1108 22-23 0.22629816200819874 1108 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58-59 0.38230780018118793 2316 60-61 0.5619121983575113 2316 62-63 0.6700972731818453 2316 64-65 0.8824408782624786 2316 66-67 1.1675145644318938 2316 68-69 1.4599746194763696 2316 70-71 1.6769895606136522 2316 72-73 1.696395697119975 2316 74-75 1.9340663638033817 2316 76-77 2.015024260993126 2316 78-79 2.047417941437473 2316 80-81 2.094155951194402 2316 82-83 2.3518912033313875 2316 84-85 2.5443305352368633 2316 86-87 2.662998622884775 2316 88-89 2.9592372989847746 2316 90-91 3.2056504037779803 2316 92-93 3.2866736133687375 2316 94-95 3.8277038700856956 2316 96-97 4.086709707042402 2316 98-99 4.640179623060714 2316 100-101 4.9571382595603914 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 6.0 4 15.0 5 36.0 6 76.0 7 221.0 8 471.0 9 1031.0 10 1902.0 11 3151.0 12 4767.0 13 7254.0 14 9959.0 15 13418.0 16 17446.0 17 23731.0 18 24782.0 19 20788.0 20 20503.0 21 22111.0 22 24316.0 23 26940.0 24 29999.0 25 33962.0 26 38316.0 27 44684.0 28 53088.0 29 63652.0 30 76026.0 31 90929.0 32 109487.0 33 135698.0 34 176404.0 35 238342.0 36 331611.0 37 413102.0 38 224877.0 39 10355.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.839228572014086 18.850723862319875 15.177585317561537 23.132462248104503 2 15.59087477874003 19.61945247451395 40.49963434050683 24.29003840623919 3 19.517982665933552 23.448168507671788 29.70808008884614 27.325768737548522 4 13.090834728260608 16.689812503460974 36.59223808541708 33.62711468286134 5 13.913458373603424 37.01439071897718 34.2673375292804 14.804813378138991 6 32.65271685575506 36.02452190140661 16.933715870344372 14.38904537249396 7 28.890877150298174 32.40137939995492 20.12046422415729 18.587279225589622 8 26.244322978073264 35.485703671664076 19.097815698230093 19.172157652032563 9 25.841817236046303 17.013544158806276 20.02253362930523 37.12210497584219 10-11 25.117976717284492 26.57913084198946 26.788290709979375 21.514601730746673 12-13 25.94944932571341 23.63190392162762 27.457519443321686 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22.149368132365275 26.53325167589964 28.762246707339862 22.555133484395228 40-41 22.684080694316837 26.248262834313095 28.751067809093396 22.316588662276676 42-43 22.306873212757996 26.2422939837041 28.72853892448475 22.722293879053154 44-45 22.38116998440294 26.530807571877503 28.69575530624872 22.392267137470835 46-47 22.625826207639896 26.716783062803334 28.130301757910857 22.52708897164591 48-49 22.47136532951727 26.722189738565426 28.186941046741147 22.619503885176158 50-51 22.594846827567594 26.978331002794466 27.973682569705787 22.453139599932154 52-53 22.76323861622238 26.803791300571493 27.901499088059488 22.531470995146634 54-55 22.609148357002802 26.915696408179603 27.9100451239415 22.565110110876095 56-57 22.536168060485032 27.06818518744604 27.850235016089226 22.545411735979698 58-59 22.560706286263425 26.79513625912976 27.806271831088473 22.837885623518343 60-61 22.45505809778322 27.045936250932545 27.84492520256503 22.654080448719206 62-63 22.373500138001038 27.0043615304555 28.010722658898494 22.61141567264497 64-65 22.262183660415417 26.9654901478595 27.865941474478966 22.906384717246116 66-67 22.21999124030749 26.991603732265602 27.790375195201833 22.998029832225072 68-69 22.50195285546384 26.778962487079976 27.91751591988703 22.801568737569145 70-71 22.551615222582814 26.751926455256925 27.601864605502342 23.094593716657926 72-73 22.513468184867367 26.92985120083821 27.47361465348978 23.083065960804642 74-75 22.38900072859316 27.189147755292108 27.382174800443522 23.039676715671206 76-77 22.49885653167854 26.770719013752576 27.657893463198196 23.072530991370684 78-79 22.532343039624394 26.879419357904933 27.66297312228113 22.925264480189547 80-81 22.52161964125359 26.971362246144853 27.477715424107302 23.029302688494255 82-83 22.55547203114317 27.003982719167286 27.53916878287684 22.90137646681271 84-85 22.58767813223084 26.978294373874323 27.428684426310816 23.005343067584015 86-87 22.67614640115794 26.951009283881216 27.315318175089338 23.057526139871502 88-89 22.65994494783212 27.079575366662002 27.0786815210723 23.18179816443358 90-91 22.558028244245683 26.997705359430103 27.248251767309274 23.19601462901494 92-93 22.585777564998924 26.736883688015624 27.321872924806655 23.355465822178793 94-95 22.44394442372127 26.737739760506013 27.321748074579876 23.496567741192838 96-97 22.651646552934476 26.57633146105139 27.276257075560135 23.495764910453996 98-99 22.496807227155813 26.592407719163308 27.058406486964458 23.852378566716418 100-101 22.873988264897463 26.142454569752875 27.161482144235283 23.82207502111438 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 611.0 1 405.5 2 288.0 3 403.0 4 341.0 5 320.0 6 637.0 7 1470.0 8 2528.5 9 3039.0 10 2619.5 11 2004.5 12 1960.0 13 2187.0 14 2350.0 15 2571.5 16 2734.0 17 2851.5 18 3130.0 19 3459.0 20 3895.0 21 4611.5 22 5646.5 23 7023.0 24 8754.0 25 11000.0 26 13821.0 27 16930.0 28 19733.0 29 22799.0 30 26167.5 31 30021.5 32 35042.5 33 38359.5 34 40607.0 35 44148.0 36 48593.5 37 52983.0 38 56682.5 39 59874.5 40 63389.0 41 67722.0 42 70220.0 43 72848.5 44 77089.0 45 79968.5 46 81595.5 47 85000.0 48 87418.0 49 87174.0 50 86086.0 51 84111.0 52 82537.5 53 81645.5 54 81409.5 55 77454.5 56 70973.0 57 65031.5 58 59860.0 59 53628.0 60 43910.5 61 35648.0 62 29454.5 63 22380.5 64 16361.5 65 12546.0 66 9371.5 67 7058.0 68 5473.5 69 4107.5 70 3025.5 71 2036.5 72 1396.0 73 1049.5 74 765.0 75 518.5 76 354.0 77 213.0 78 117.5 79 77.0 80 57.5 81 32.5 82 19.0 83 12.0 84 7.0 85 5.5 86 3.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014999165017412549 3 0.002528928985493976 4 0.0013952711644104696 5 0.11681035779548901 6 4.3602223887827176E-5 7 0.0 8 2.1801111943913588E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.1801111943913588E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.5260778360739512E-4 24-25 5.232266866539261E-4 26-27 0.0044692279485022855 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010246522613639387 36-37 4.796244627660989E-4 38-39 0.006126112456239717 40-41 0.009330875911995015 42-43 0.005363073538202743 44-45 0.003488177911026174 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 4.3602223887827176E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 6.540333583174076E-5 68-69 0.0010682544852517659 70-71 0.0 72-73 2.1801111943913588E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.0900555971956794E-4 82-83 0.0023109178660548403 84-85 0.005036056859044039 86-87 0.003379172351306606 88-89 0.0 90-91 0.0032047634557552974 92-93 0.0 94-95 2.1801111943913588E-5 96-97 1.3080667166348153E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2293461.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.019919590825936 #Duplication Level Percentage of deduplicated Percentage of total 1 74.25700066086983 40.856142054169666 2 14.33093814702732 15.769741290210865 3 4.905488816183186 8.096988006602844 4 2.202367309325586 4.846962890742297 5 1.2190526134458934 3.3536088384389635 6 0.7485701100596769 2.4711760358147488 7 0.4692580156911956 1.8072976803476082 8 0.33269438058413675 1.4643854454447078 9 0.23928520342817178 1.1848907386703194 >10 1.1896790503415127 11.578546762729433 >50 0.0692138264360064 2.609599247698314 >100 0.03256440191808553 3.3659377307884264 >500 0.0026974244782542083 0.9606189994865338 >1k 0.0010313681828615228 0.9280119929603172 >5k 1.5867202813254198E-4 0.7060922858949243 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8630 0.37628719215194856 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7546 0.32902238145754387 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4490 0.19577398525634399 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.3602223887827176E-5 2 0.0 0.0 0.0 0.0 4.3602223887827176E-5 3 0.0 0.0 0.0 0.0 4.3602223887827176E-5 4 0.0 0.0 0.0 4.3602223887827176E-5 4.3602223887827176E-5 5 0.0 0.0 0.0 4.3602223887827176E-5 4.3602223887827176E-5 6 0.0 0.0 0.0 4.3602223887827176E-5 4.3602223887827176E-5 7 0.0 0.0 0.0 4.3602223887827176E-5 4.3602223887827176E-5 8 0.0 0.0 0.0 4.3602223887827176E-5 4.3602223887827176E-5 9 0.0 0.0 0.0 4.3602223887827176E-5 4.3602223887827176E-5 10-11 4.3602223887827176E-5 0.0 0.0 4.3602223887827176E-5 4.3602223887827176E-5 12-13 8.720444777565435E-5 0.0 0.0 8.720444777565435E-5 3.0521556721479023E-4 14-15 1.744088955513087E-4 0.0 0.0 1.3080667166348153E-4 3.488177911026174E-4 16-17 2.1801111943913588E-4 0.0 0.0 2.1801111943913588E-4 3.7061890304653097E-4 18-19 2.1801111943913588E-4 0.0 0.0 2.834144552708766E-4 3.924200149904446E-4 20-21 2.1801111943913588E-4 0.0 0.0 3.488177911026174E-4 5.014255747100124E-4 22-23 2.6161334332696305E-4 0.0 0.0 3.924200149904446E-4 5.668289105417532E-4 24-25 3.0521556721479023E-4 0.0 0.0 4.7962446276609893E-4 5.668289105417532E-4 26-27 3.0521556721479023E-4 0.0 0.0 7.630389180369756E-4 6.976355822052348E-4 28-29 3.924200149904446E-4 0.0 0.0 0.0030521556721479024 6.976355822052348E-4 30-31 3.924200149904446E-4 0.0 0.0 0.010878754860012881 6.976355822052348E-4 32-33 3.924200149904446E-4 0.0 0.0 0.025659908757986294 6.976355822052348E-4 34-35 4.142211269343582E-4 0.0 0.0 0.049466723000739926 7.848400299808892E-4 36-37 4.3602223887827176E-4 0.0 0.0 0.08914474673866266 7.848400299808892E-4 38-39 4.3602223887827176E-4 0.0 0.0 0.14489018997924968 8.066411419248027E-4 40-41 4.578233508221853E-4 0.0 0.0 0.20532287228777818 8.284422538687163E-4 42-43 5.450277985978397E-4 0.0 0.0 0.2666275990740632 8.502433658126298E-4 44-45 6.540333583174076E-4 0.0 0.0 0.34003194298922024 9.156467016443706E-4 46-47 7.194366941491484E-4 0.0 0.0 0.4302231431011907 9.810500374761114E-4 48-49 7.412378060930619E-4 0.0 0.0 0.5226380566314405 0.001002851149420025 50-51 7.412378060930619E-4 0.0 0.0 0.6208084637148833 0.0010464533733078522 52-53 7.412378060930619E-4 0.0 0.0 0.71498926731259 0.0011772600449713338 54-55 7.412378060930619E-4 0.0 0.0 0.8155141944859756 0.0011772600449713338 56-57 7.412378060930619E-4 0.0 0.0 0.9325425634009037 0.0011772600449713338 58-59 7.412378060930619E-4 0.0 0.0 1.0608857094147228 0.0011990611569152473 60-61 7.848400299808892E-4 0.0 0.0 1.1925208233320732 0.001220862268859161 62-63 8.284422538687163E-4 0.0 0.0 1.3195995048531455 0.001264464492746988 64-65 8.284422538687163E-4 0.0 0.0 1.4568811067639693 0.0012862656046909015 66-67 9.156467016443706E-4 0.0 0.0 1.6092926803638692 0.0013080667166348152 68-69 9.156467016443706E-4 0.0 0.0 1.7752427444809395 0.0013080667166348152 70-71 9.156467016443706E-4 0.0 0.0 1.9528782045999473 0.0013298678285787288 72-73 9.374478135882843E-4 0.0 0.0 2.1306444713906187 0.001417072276354383 74-75 9.592489255321979E-4 0.0 0.0 2.317567205197734 0.0014824756121861239 76-77 9.592489255321979E-4 0.0 0.0 2.517178186156207 0.0014824756121861239 78-79 9.592489255321979E-4 0.0 0.0 2.7363665656403136 0.0015042767241300375 80-81 0.0010900555971956793 0.0 0.0 2.9637739643272765 0.0015696800599617783 82-83 0.0010900555971956793 0.0 0.0 3.1995311888887583 0.0018094922913448278 84-85 0.001111856709139593 0.0 0.0 3.434961396771081 0.0018748956271765686 86-87 0.0011336578210835065 0.0 0.0 3.6797878839012306 0.0020275034107839636 88-89 0.0011336578210835065 0.0 0.0 3.9462192729677987 0.0021801111943913587 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4670 0.0 54.387962 1 GTATCAA 8270 0.0 40.547306 1 ATCAACG 10200 0.0 32.728794 3 TCAACGC 10315 0.0 32.224194 4 TATCAAC 10440 0.0 32.16493 2 CAACGCA 10435 0.0 31.762484 5 AACGCAG 10855 0.0 30.583761 6 ACGCAGA 12340 0.0 26.826326 7 CGCAGAG 12480 0.0 26.52539 8 TGGTATC 2075 0.0 23.610085 2 GCAGAGT 13985 0.0 23.433125 9 GAGTACT 8500 0.0 22.909124 12-13 TACATGG 7760 0.0 22.494831 2 GTACATG 8120 0.0 22.024712 1 GTACTTT 8770 0.0 21.878895 14-15 CAGAGTA 13030 0.0 21.797182 10-11 GTGGTAT 2435 0.0 21.682154 1 ACATGGG 7925 0.0 21.12207 3 AGAGTAC 12245 0.0 20.789764 10-11 AGTACTT 8715 0.0 19.973316 12-13 >>END_MODULE