FastQCFastQC Report
Thu 26 May 2016
SRR938234_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938234_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2279722
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89860.39417086820235103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74340.3260923919670907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46250.20287561378097857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43150.056.8955231
GTATCAA78350.042.0025861
ATCAACG97950.033.335463
TCAACGC98650.033.14714
TATCAAC100700.032.51952
CAACGCA101000.032.3287935
AACGCAG104900.031.2545556
GTGGTAT19800.029.9124721
CGCAGAG119050.027.2537448
ACGCAGA119800.027.2417357
TGGTATC18850.027.2311882
GCAGAGT130900.024.242189
TACATGG70300.024.068492
GTACATG74550.023.4492651
ACATGGG73550.022.7464773
GAGTACT85350.022.40155612-13
CAGAGTA122000.022.34974710-11
AGAGTAC115700.021.57545310-11
GTACTTT88900.021.3743614-15
CATGGGG48000.020.1996154
Produced by FastQC (version 0.11.5)