##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938234_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2279722 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.878542646866592 33.0 31.0 34.0 30.0 34.0 2 32.075203906441224 34.0 31.0 34.0 30.0 34.0 3 32.15839387434082 34.0 31.0 34.0 30.0 34.0 4 35.669212737342534 37.0 35.0 37.0 35.0 37.0 5 35.67603506041526 37.0 35.0 37.0 35.0 37.0 6 35.69548172978986 37.0 35.0 37.0 35.0 37.0 7 35.6411970406918 37.0 35.0 37.0 33.0 37.0 8 35.61746037455444 37.0 35.0 37.0 33.0 37.0 9 37.259243451613834 39.0 37.0 39.0 34.0 39.0 10-11 37.16927809618892 39.0 37.0 39.0 34.0 39.0 12-13 37.06900797553386 39.0 37.0 39.0 33.0 39.0 14-15 38.263939857579125 40.0 38.0 41.0 33.0 41.0 16-17 38.11282976608551 40.0 38.0 41.0 33.0 41.0 18-19 38.25376142354199 40.0 38.0 41.0 33.5 41.0 20-21 38.23499597757972 40.0 38.0 41.0 34.0 41.0 22-23 38.14836304602052 40.0 38.0 41.0 33.5 41.0 24-25 38.04512567760455 40.0 38.0 41.0 33.0 41.0 26-27 37.86909456503908 40.0 38.0 41.0 33.0 41.0 28-29 37.663435717162 40.0 38.0 41.0 32.5 41.0 30-31 37.452590710621735 40.0 37.0 41.0 32.0 41.0 32-33 37.203155691790485 40.0 37.0 41.0 31.0 41.0 34-35 36.969930105512866 40.0 36.0 41.0 31.0 41.0 36-37 36.71420309142957 39.0 36.0 41.0 30.0 41.0 38-39 36.42557908376548 39.0 35.5 41.0 30.0 41.0 40-41 36.215944093183296 39.0 35.0 40.5 30.0 41.0 42-43 36.04342152244879 39.0 35.0 41.0 29.0 41.0 44-45 35.781627540551 39.0 35.0 40.5 27.5 41.0 46-47 35.40648750154624 39.0 35.0 40.0 26.5 41.0 48-49 35.02680699664258 38.0 34.5 40.0 24.5 41.0 50-51 34.33532509665652 37.5 33.5 39.5 24.0 40.5 52-53 34.46337557825033 38.0 34.0 40.0 24.0 40.5 54-55 34.574280767567274 38.0 34.0 40.0 24.0 41.0 56-57 34.27099005931425 38.0 34.0 40.0 23.0 41.0 58-59 33.94815091489225 38.0 33.5 40.0 22.5 41.0 60-61 33.53075462709927 37.0 33.0 40.0 20.5 41.0 62-63 32.99037667750717 36.5 32.5 39.5 18.5 41.0 64-65 32.46659833962211 36.0 31.5 39.0 14.5 40.5 66-67 31.925560879791483 35.5 31.0 39.0 9.5 40.0 68-69 31.319270068894365 35.0 30.5 38.0 7.0 40.0 70-71 30.764018156599796 35.0 30.0 38.0 2.0 40.0 72-73 30.11487957742216 34.5 29.0 37.0 2.0 39.0 74-75 29.416586101287788 34.0 28.5 36.5 2.0 39.0 76-77 28.736074398545085 34.0 27.5 36.0 2.0 38.5 78-79 28.010751310905455 33.5 26.0 35.0 2.0 37.5 80-81 27.355195501907687 33.0 25.5 35.0 2.0 37.0 82-83 26.60934425337826 32.5 24.5 35.0 2.0 36.0 84-85 25.940289649351982 32.0 22.0 35.0 2.0 36.0 86-87 25.24368497562422 31.5 19.0 34.5 2.0 35.5 88-89 24.42593746079566 31.0 15.0 34.0 2.0 35.0 90-91 23.584535746025175 31.0 4.5 34.0 2.0 35.0 92-93 22.691907171137533 30.0 2.0 34.0 2.0 35.0 94-95 21.73621849506212 29.0 2.0 34.0 2.0 35.0 96-97 20.52243102448456 29.0 2.0 34.0 2.0 35.0 98-99 19.031059050182435 26.0 2.0 33.5 2.0 35.0 100-101 15.375916449461819 11.0 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.31910053448910247 1101 2 0.2777007738176849 1101 3 0.26878625367263 1101 4 0.15233232137840247 1101 5 0.24250666500008577 1101 6 0.1367926550967269 1101 7 0.17651936808515956 1101 8 0.11455042886618116 1101 9 0.14148364676810132 1101 10-11 0.2573051761807932 1101 12-13 0.39213867433091565 1101 14-15 0.5302466688164813 1101 16-17 0.6152070987867617 1101 18-19 0.5396297666190577 1101 20-21 0.5674160602000384 1101 22-23 0.5461562239545472 1101 24-25 0.4234401515714197 1101 26-27 0.48945823479842687 1101 28-29 0.6469957763146965 1101 30-31 0.674203973203717 1101 32-33 0.6556317646359631 1101 34-35 0.8014062041314745 1101 36-37 0.5936325028978544 1101 38-39 0.8318572889653737 1101 40-41 0.9721200824708589 1101 42-43 1.2812305669893078 1101 44-45 1.167979539263719 1101 46-47 1.106664306015439 1101 48-49 1.1953912640555657 1101 50-51 1.0325283878356473 1101 52-53 1.141733973208396 1101 54-55 1.331281066050913 1101 56-57 1.271958567690767 1101 58-59 1.2938049172192265 1101 60-61 1.2229572154176722 1101 62-63 1.2290487922409952 1101 64-65 1.1532869165908437 1101 66-67 1.2731160471762912 1101 68-69 1.5822130975987356 1101 70-71 1.7324501607115401 1101 72-73 2.0321994650290627 1101 74-75 2.153414002554257 1101 76-77 2.2451748269944893 1101 78-79 2.3073169079626368 1101 80-81 2.34877106588738 1101 82-83 2.4540270067404144 1101 84-85 2.6765951765838913 1101 86-87 2.8374165013205577 1101 88-89 3.150470075700884 1101 90-91 3.486861908038396 1101 92-93 3.561541303656565 1101 94-95 3.712915790161219 1101 96-97 3.9772247444433155 1101 98-99 4.168722270873609 1101 100-101 3.942892588116619 1102 1 0.34081401059318495 1102 2 0.23280109972073149 1102 3 0.17825316503591182 1102 4 0.21646385658178247 1102 5 0.2920987839289353 1102 6 0.2850807250885765 1102 7 0.14793318983340242 1102 8 0.21291139247702517 1102 9 0.3838704345485269 1102 10-11 0.3615301672055793 1102 12-13 0.3449124315798784 1102 14-15 0.5036675764009999 1102 16-17 0.5026738074979207 1102 18-19 0.48911523113353184 1102 20-21 0.5241782208560011 1102 22-23 0.45895291932735205 1102 24-25 0.5001752075060253 1102 26-27 0.5617652981857333 1102 28-29 0.594089596385821 1102 30-31 0.6042140883398233 1102 32-33 0.4745420028482954 1102 34-35 0.41335425006711546 1102 36-37 0.5479558500819834 1102 38-39 0.49384403097436547 1102 40-41 0.609619110701523 1102 42-43 0.6482833500056628 1102 44-45 0.6498570238267831 1102 46-47 0.6936640850198046 1102 48-49 0.772888667575252 1102 50-51 0.9329919108903795 1102 52-53 0.8303688141745269 1102 54-55 0.8838976805550374 1102 56-57 0.9104496741451484 1102 58-59 0.8816377973513667 1102 60-61 1.0109870262436615 1102 62-63 1.0616356465821681 1102 64-65 1.072596213519006 1102 66-67 1.0374783612189482 1102 68-69 1.2262435076460854 1102 70-71 1.3854800283150688 1102 72-73 1.606681763104124 1102 74-75 1.5858362893961058 1102 76-77 1.5832826414175578 1102 78-79 1.843366493093562 1102 80-81 1.949716935933214 1102 82-83 2.304880722480636 1102 84-85 2.394018629548942 1102 86-87 2.574385183706159 1102 88-89 2.871553932624991 1102 90-91 2.93172917526579 1102 92-93 3.219283269853843 1102 94-95 3.538555476434766 1102 96-97 3.6515429117693294 1102 98-99 3.7501547942681377 1102 100-101 3.7436763801396378 1103 1 0.6563349804141154 1103 2 0.5574791221552999 1103 3 0.4958535400106072 1103 4 0.37942019120409043 1103 5 0.5246656561925249 1103 6 0.4978692798380777 1103 7 0.5363084415032091 1103 8 0.5005904908333179 1103 9 0.5733322606327036 1103 10-11 0.6954358163272545 1103 12-13 0.7010345327165268 1103 14-15 0.9510581999029668 1103 16-17 1.0181204748037445 1103 18-19 0.9958551883097186 1103 20-21 0.9547623611306264 1103 22-23 1.0066378233998776 1103 24-25 1.024238303390888 1103 26-27 1.0222366264696916 1103 28-29 1.1007557836814215 1103 30-31 1.1352126182685538 1103 32-33 1.1982951054443518 1103 34-35 1.3259585132737897 1103 36-37 1.2794215004819947 1103 38-39 1.3192656111944459 1103 40-41 1.2693079988802367 1103 42-43 1.3310363780328842 1103 44-45 1.217938101452937 1103 46-47 1.488675962769527 1103 48-49 1.5527116731253017 1103 50-51 1.5489155524060507 1103 52-53 1.4749194304326565 1103 54-55 1.6159103466038474 1103 56-57 1.6137693187713964 1103 58-59 1.6782467796998688 1103 60-61 1.755123519164954 1103 62-63 1.6540013960521947 1103 64-65 1.7964041774703006 1103 66-67 1.9938270983425035 1103 68-69 2.0282965473912746 1103 70-71 2.218863162975719 1103 72-73 2.2819611299082077 1103 74-75 2.2187890553671537 1103 76-77 2.3813465149488167 1103 78-79 2.5418524794794237 1103 80-81 2.710355777772719 1103 82-83 2.6670799171372437 1103 84-85 2.879942430854964 1103 86-87 2.8239817130596485 1103 88-89 3.1615523879848944 1103 90-91 3.5877079977173985 1103 92-93 3.7205525379074906 1103 94-95 3.903512386302353 1103 96-97 4.0659024720262735 1103 98-99 4.380513630142136 1103 100-101 4.436715645956218 1104 1 0.20311571069468215 1104 2 0.13577578520864364 1104 3 0.18380166450400992 1104 4 0.12183857419611144 1104 5 0.16963533363077943 1104 6 0.15068522530629735 1104 7 0.18487581032517397 1104 8 0.15137907275305906 1104 9 0.23485190437922654 1104 10-11 0.242470699480414 1104 12-13 0.2661816532938772 1104 14-15 0.4546427075641759 1104 16-17 0.5821608951203459 1104 18-19 0.5082127308638462 1104 20-21 0.4451267689861709 1104 22-23 0.42814364865207466 1104 24-25 0.41390397395407774 1104 26-27 0.4622582575403129 1104 28-29 0.4471256212774932 1104 30-31 0.4474639586136746 1104 32-33 0.3799582564516939 1104 34-35 0.4783799236391246 1104 36-37 0.53349502659065 1104 38-39 0.7028054895135725 1104 40-41 0.6909168524823244 1104 42-43 0.7492658041015048 1104 44-45 0.8290629659535682 1104 46-47 0.8007424868566773 1104 48-49 1.0519892539610538 1104 50-51 0.9194167291753459 1104 52-53 0.8005554229716481 1104 54-55 0.959701057450637 1104 56-57 1.054414002801515 1104 58-59 1.1804760495169475 1104 60-61 1.1775717364326965 1104 62-63 1.2219639738117465 1104 64-65 1.358301022166053 1104 66-67 1.3561966039040847 1104 68-69 1.4854946157317919 1104 70-71 1.6398338267517367 1104 72-73 1.7967610791264654 1104 74-75 1.8736633705460122 1104 76-77 2.0046587543261545 1104 78-79 2.3170927162004844 1104 80-81 2.3581540961156726 1104 82-83 2.659434090517845 1104 84-85 2.834840672048724 1104 86-87 2.789788263904871 1104 88-89 2.9413810484210323 1104 90-91 3.4209158415440015 1104 92-93 3.6055616688560335 1104 94-95 3.8673982640941276 1104 96-97 4.1277656249867505 1104 98-99 4.181509977762719 1104 100-101 4.141575800840544 1105 1 0.2920108133837047 1105 2 0.18663669526747384 1105 3 0.10891915909979843 1105 4 0.039277604269713606 1105 5 0.052550131676298406 1105 6 0.21500881133815142 1105 7 0.1543573284521429 1105 8 0.23936540717261323 1105 9 0.3297341229926616 1105 10-11 0.1512520537781583 1105 12-13 0.2402313899840962 1105 14-15 0.26556423802314555 1105 16-17 0.3337723007894837 1105 18-19 0.25825879663213414 1105 20-21 0.32336777361425106 1105 22-23 0.32442845340133175 1105 24-25 0.33105119441373176 1105 26-27 0.3954542897899387 1105 28-29 0.456084606611725 1105 30-31 0.5188187582365416 1105 32-33 0.6171842368280309 1105 34-35 0.5609796103202029 1105 36-37 0.7321532042503662 1105 38-39 0.6481103660322205 1105 40-41 0.6775031101853486 1105 42-43 0.8257075551025821 1105 44-45 0.8133513125688978 1105 46-47 0.7947431823243036 1105 48-49 0.7816473635079575 1105 50-51 0.8826417272932829 1105 52-53 0.9049354350885608 1105 54-55 1.0010369288823284 1105 56-57 1.0924722141591516 1105 58-59 1.0341794146482144 1105 60-61 1.1663813396218075 1105 62-63 1.113567815374175 1105 64-65 1.2313416138154238 1105 66-67 1.376778502387321 1105 68-69 1.4508812993191107 1105 70-71 1.5809866898198912 1105 72-73 1.6259375594149752 1105 74-75 1.7544394263841738 1105 76-77 1.9778036661200638 1105 78-79 1.9875930585569748 1105 80-81 2.08785468654618 1105 82-83 2.257909976790959 1105 84-85 2.36808079838006 1105 86-87 2.553659437488573 1105 88-89 2.8131346664659027 1105 90-91 2.9549566518713384 1105 92-93 3.188885143756462 1105 94-95 3.537565929151185 1105 96-97 3.8068343794902617 1105 98-99 3.9057755245114087 1105 100-101 3.971733105580837 1106 1 0.20078754226877749 1106 2 0.0538701215708457 1106 3 0.01555936506418476 1106 4 0.12067286994946613 1106 5 0.17172877717440116 1106 6 -0.057824271691238494 1106 7 0.13106297101811037 1106 8 0.06083790949686119 1106 9 0.0022042653083289565 1106 10-11 0.14967066923978933 1106 12-13 0.21022262934617686 1106 14-15 0.2650850847656159 1106 16-17 0.04881646155613595 1106 18-19 0.16175232448738797 1106 20-21 0.1001476134197361 1106 22-23 0.1400125555253382 1106 24-25 0.10764956386234559 1106 26-27 0.04848833112584572 1106 28-29 0.06845498492825897 1106 30-31 0.14814365275131536 1106 32-33 0.19331507427350658 1106 34-35 0.2469330895449815 1106 36-37 0.2292291178597523 1106 38-39 0.06221320519406248 1106 40-41 0.3418240713524696 1106 42-43 0.36383152706854105 1106 44-45 0.4016683761460982 1106 46-47 0.5775616046943313 1106 48-49 0.6080204594101275 1106 50-51 0.510784829631838 1106 52-53 0.5374803110093254 1106 54-55 0.10821260649085929 1106 56-57 0.5373374076083408 1106 58-59 0.7670666706018707 1106 60-61 0.8192526833963996 1106 62-63 0.6967152086374284 1106 64-65 0.8581589992119731 1106 66-67 1.0707801472417877 1106 68-69 0.9953674610869676 1106 70-71 0.9452414992313223 1106 72-73 1.2557094252520642 1106 74-75 1.4045064720825238 1106 76-77 1.665308135833289 1106 78-79 1.9548711819925728 1106 80-81 2.0294820197364842 1106 82-83 2.046280676926841 1106 84-85 2.330356418776258 1106 86-87 2.2290956814991567 1106 88-89 2.389800975555513 1106 90-91 2.7366218882297737 1106 92-93 2.9599276596686437 1106 94-95 3.2631022947379655 1106 96-97 3.295651157005814 1106 98-99 3.175526293302081 1106 100-101 3.2699613112591575 1107 1 0.23605616774895566 1107 2 0.15431656858236664 1107 3 0.029482866695765608 1107 4 0.13746850254801046 1107 5 0.0887780963168936 1107 6 0.11880730798079497 1107 7 0.13046093877203901 1107 8 0.19516923720343016 1107 9 0.04887639352158146 1107 10-11 0.09578293155924911 1107 12-13 0.11949630102002828 1107 14-15 0.2623568357190962 1107 16-17 0.27794824775723725 1107 18-19 0.19012253473398744 1107 20-21 0.22793971437114635 1107 22-23 0.21965102638409206 1107 24-25 0.3721950622169956 1107 26-27 0.2752369439389071 1107 28-29 0.4179100923922192 1107 30-31 0.48538979409980954 1107 32-33 0.42114331781258585 1107 34-35 0.48635656363934743 1107 36-37 0.46273312452375137 1107 38-39 0.5371433295245609 1107 40-41 0.4996246744173689 1107 42-43 0.614721495263062 1107 44-45 0.6230425861723958 1107 46-47 0.7791243413422251 1107 48-49 0.894274228565699 1107 50-51 0.7936315575229074 1107 52-53 0.828406456213024 1107 54-55 0.9118824560000078 1107 56-57 0.913709793294494 1107 58-59 0.8540623954500717 1107 60-61 0.9213193509809656 1107 62-63 0.9603605844515002 1107 64-65 1.0585312678649927 1107 66-67 1.2065251664489942 1107 68-69 1.3530407045572126 1107 70-71 1.4727818068219527 1107 72-73 1.6818649554121947 1107 74-75 1.6337019380809537 1107 76-77 1.803353200476888 1107 78-79 1.925674855456343 1107 80-81 2.250115995145755 1107 82-83 1.9976725107673197 1107 84-85 2.0168101769169446 1107 86-87 2.0890486617136723 1107 88-89 2.3024202460948295 1107 90-91 2.638500270143332 1107 92-93 2.810666173210066 1107 94-95 3.292665210377727 1107 96-97 3.381330775340089 1107 98-99 3.7293625708220794 1107 100-101 3.596212877437962 1108 1 0.21282400862727968 1108 2 0.13934606747051959 1108 3 0.08546691514122529 1108 4 0.09847975233261508 1108 5 0.027803935537697555 1108 6 0.10846489112334723 1108 7 0.08354285459969191 1108 8 0.0845821313766777 1108 9 0.15183104154527882 1108 10-11 0.09253961291381785 1108 12-13 0.15603627426998656 1108 14-15 0.27147268962779947 1108 16-17 0.26476450127535855 1108 18-19 0.30105895006519745 1108 20-21 0.24504275123859998 1108 22-23 0.24173186728702234 1108 24-25 0.17322994393425262 1108 26-27 0.2904306355257731 1108 28-29 0.39829148552746574 1108 30-31 0.36208304669070657 1108 32-33 0.3807106401012632 1108 34-35 0.31767568499095944 1108 36-37 0.44718708738551527 1108 38-39 0.29408274808055523 1108 40-41 0.43997849696452107 1108 42-43 0.4075410802272472 1108 44-45 0.5983020861272692 1108 46-47 0.712524795928708 1108 48-49 0.7690108720107105 1108 50-51 0.7710715955965739 1108 52-53 0.7161628996071627 1108 54-55 0.7127285284220335 1108 56-57 0.9017613076254563 1108 58-59 0.7659183227162387 1108 60-61 0.9574310775069961 1108 62-63 1.0310860355506222 1108 64-65 1.091630579420844 1108 66-67 1.1016832460262371 1108 68-69 1.2993188392756387 1108 70-71 1.474326416197595 1108 72-73 1.5795752016602158 1108 74-75 1.618111591068999 1108 76-77 1.603819520870097 1108 78-79 1.8737943115936702 1108 80-81 2.0074404172711517 1108 82-83 2.03212171442042 1108 84-85 2.1379030717466385 1108 86-87 2.4072715075563345 1108 88-89 2.678774610207114 1108 90-91 2.8907487500685 1108 92-93 3.0161847218155344 1108 94-95 3.14179173147825 1108 96-97 3.2944587242032135 1108 98-99 3.417624392942553 1108 100-101 3.443108888728771 1109 1 0.2031443343578303 1109 2 0.07967492080769745 1109 3 0.10818013533364024 1109 4 0.113982220534254 1109 5 0.07510164509462669 1109 6 0.17836868530775973 1109 7 0.13581299956900494 1109 8 0.034954343389792086 1109 9 0.1445371754166871 1109 10-11 0.1863295935765663 1109 12-13 0.12523191678311463 1109 14-15 0.2441021951467235 1109 16-17 0.2779203162623318 1109 18-19 0.2086177725348506 1109 20-21 0.2616490040160784 1109 22-23 0.1985423021949586 1109 24-25 0.15181460144875558 1109 26-27 0.1797574350309361 1109 28-29 0.21539113841784285 1109 30-31 0.27029981604897557 1109 32-33 0.1722844038985798 1109 34-35 0.1551697697145329 1109 36-37 0.30156775494096877 1109 38-39 0.26345903172556717 1109 40-41 0.32807732111567134 1109 42-43 0.4257351493368944 1109 44-45 0.5354272017303856 1109 46-47 0.7297976433085651 1109 48-49 0.7028008526734553 1109 50-51 0.7586460319938411 1109 52-53 0.7988790023708319 1109 54-55 0.9108072946815398 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2316 100-101 -3.1688274963991017 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6501.0 3 1917.0 4 2021.0 5 2238.0 6 2676.0 7 3245.0 8 3917.0 9 5069.0 10 6409.0 11 8013.0 12 10171.0 13 12455.0 14 14437.0 15 17431.0 16 20732.0 17 25492.0 18 24674.0 19 21126.0 20 21742.0 21 23292.0 22 25335.0 23 28530.0 24 31749.0 25 35937.0 26 40453.0 27 46670.0 28 53752.0 29 63144.0 30 73925.0 31 88612.0 32 109206.0 33 136813.0 34 177551.0 35 241109.0 36 320575.0 37 363739.0 38 197200.0 39 11863.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.56097481434343 18.41916805230599 14.823924280411385 23.195932852939194 2 15.416630097070335 19.34451192753954 41.02402183643736 24.214836138952762 3 19.535616977051205 23.540288545265582 29.36943484733575 27.554659630347462 4 12.887086622605851 16.37252311525014 36.83315147785738 33.90723878428663 5 13.992240727968458 36.9262149161183 34.20463071458268 14.87691364133057 6 32.64533978873317 35.84327392029882 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22.130277205010934 26.72193022511291 29.0098134580618 22.137979111814353 30-31 22.312211965892985 26.267964485101114 29.006408779452936 22.41341476955297 32-33 22.04911892349492 26.218921607823635 29.031836193015724 22.700123275665717 34-35 22.58673215351959 26.395711959960032 28.84262549848619 22.17493038803418 36-37 22.602576657796646 26.154987855745254 28.79378772382501 22.448647762633087 38-39 22.18239122169601 26.523256051197997 28.717195825408876 22.577156901697116 40-41 22.68904373716763 26.286088861695493 28.707782753076422 22.317084648060447 42-43 22.36098575908337 26.202900483823484 28.686603745189515 22.749510011903627 44-45 22.437730066261715 26.57301654118977 28.57174820855144 22.417505183997072 46-47 22.67762931579169 26.741256967748278 28.055805169823504 22.525308546636523 48-49 22.539392881024547 26.69146309144168 28.154809020434453 22.614335007099317 50-51 22.654475243511126 26.94795736701761 27.959490418810173 22.43807697066109 52-53 22.768763567381075 26.79929576846875 27.889468290277463 22.542472373872712 54-55 22.557660968444107 26.944902606526068 27.961514238902417 22.53592218612741 56-57 22.529150146148318 27.031176480265863 27.885823338762485 22.553850034823334 58-59 22.612308377378522 26.762599081110782 27.816853906704996 22.8082386348057 60-61 22.48577368327188 26.944438777535968 27.9446737165933 22.625113822598856 62-63 22.39437765962931 26.911220397348263 28.083426068694145 22.610975874328286 64-65 22.320589313397072 26.885241082853057 27.965470562518245 22.828699041231626 66-67 22.2571079560896 26.93963668874675 27.853277827142776 22.949977528020877 68-69 22.50344055764892 26.72031172746484 28.00515217201516 22.77109554287108 70-71 22.521232787161434 26.680020427700597 27.755721379501573 23.043025405636396 72-73 22.45033920308444 26.7972849713927 27.677265035561156 23.075110789961712 74-75 22.32814481067303 27.024357393864147 27.675715871351546 22.971781924111276 76-77 22.57647899307677 26.761807476512928 27.608155916750988 23.05355761365932 78-79 22.55192305540938 26.89456663390229 27.624603158760845 22.928907151927486 80-81 22.616994724041554 26.88343336763478 27.42171230967031 23.077859598653358 82-83 22.616792662711042 26.877069787469832 27.55730145669605 22.948836093123077 84-85 22.686925594766745 26.891454547927264 27.427642872779646 22.993976984526345 86-87 22.75042418755143 26.854383914737863 27.278900463754997 23.11629143395571 88-89 22.769403381785068 26.845351488051683 27.21204684415798 23.173198286005263 90-91 22.69351988266436 26.85761864381679 27.203306811412798 23.245554662106052 92-93 22.723888504022955 26.512782691825933 27.353174866065928 23.410153938085188 94-95 22.65892088215587 26.4962918789934 27.363545929930755 23.481241308919984 96-97 22.840401475972016 26.292753463573813 27.31103286668227 23.555812193771896 98-99 22.74530107662522 26.302716517942837 27.098947506979144 23.853034898452798 100-101 23.58633178386115 25.32921341552082 27.118618751205375 23.965836049412655 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 654.0 1 453.0 2 346.0 3 436.5 4 360.5 5 330.0 6 648.0 7 1508.0 8 2575.5 9 3087.5 10 2655.5 11 2051.0 12 1982.0 13 2156.0 14 2365.0 15 2552.5 16 2679.5 17 2943.0 18 3194.5 19 3383.5 20 3776.0 21 4559.5 22 5626.5 23 6862.0 24 8509.5 25 10744.0 26 13743.0 27 16935.5 28 19564.0 29 22610.0 30 25925.5 31 29814.5 32 34682.5 33 38000.5 34 40527.0 35 43807.0 36 48355.5 37 52878.0 38 56500.0 39 59631.5 40 62999.0 41 67187.0 42 69685.5 43 72440.0 44 76198.0 45 79070.5 46 81025.5 47 83928.5 48 86085.5 49 86073.0 50 85312.0 51 83623.0 52 82006.0 53 80726.0 54 79879.0 55 76010.5 56 69961.0 57 64280.0 58 59364.5 59 52872.0 60 43511.0 61 35789.5 62 29626.0 63 22914.0 64 17104.0 65 13306.5 66 10126.5 67 7600.5 68 5853.5 69 4504.0 70 3395.5 71 2372.5 72 1675.0 73 1282.5 74 947.0 75 624.0 76 428.0 77 286.5 78 179.0 79 119.0 80 76.0 81 48.0 82 35.5 83 28.0 84 18.5 85 10.0 86 9.5 87 7.5 88 7.0 89 9.0 90 5.5 91 1.5 92 2.5 93 2.5 94 1.0 95 1.0 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4655392192556812 2 0.04219812766644354 3 0.055182166948426176 4 0.0594809367107042 5 0.08470331031590693 6 0.08505423029650107 7 0.049611312256494434 8 0.041847207685849416 9 0.04956744725892017 10-11 0.0536030270357526 12-13 0.048185699835330796 14-15 0.04088217773921557 16-17 0.04134276021374536 18-19 0.0495893797577073 20-21 0.05772633680773357 22-23 0.0497867722467915 24-25 0.06921896617219117 26-27 0.05974412669614979 28-29 0.04673815491538003 30-31 0.0495235822613459 32-33 0.047768982358375275 34-35 0.052572199592757364 36-37 0.047045209898399894 38-39 0.02844645092691126 40-41 0.025156576108841342 42-43 0.025661023580945394 44-45 0.01541854664735437 46-47 0.028512248423272664 48-49 0.027634948471787348 50-51 0.01579139912673563 52-53 0.015703669131587095 54-55 0.016909956564879404 56-57 0.015769466627948495 58-59 0.014892166676463184 60-61 0.017874986511513247 62-63 0.016953821562453668 64-65 0.017173146550324998 66-67 0.00949677197482851 68-69 0.012940174284408362 70-71 0.020353358874459253 72-73 0.017940784007874643 74-75 0.01456317919465619 76-77 0.00980382695784837 78-79 0.013839406734680808 80-81 0.0184452314799787 82-83 0.01541854664735437 84-85 0.013115634274705424 86-87 0.003070549830198594 88-89 0.03151700075710986 90-91 0.03463141558488272 92-93 0.018818083959359955 94-95 0.035311323047283835 96-97 0.02989399584686203 98-99 0.026735716021514908 100-101 0.1519044865996819 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2279722.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.86283053192708 #Duplication Level Percentage of deduplicated Percentage of total 1 75.66932112657378 43.02771783686334 2 13.580688153123901 15.444727379160684 3 4.707628983046811 8.030673272105371 4 2.061958814063838 4.689952584317014 5 1.1547434572767503 3.283099075948971 6 0.6647833633077571 2.26808782369281 7 0.45016930635660085 1.791853068462139 8 0.3103833203766924 1.4119419317213353 9 0.2148817309237616 1.0996905104932508 >10 1.0906215619752204 10.898037268849626 >50 0.06115132204655093 2.3886955268590095 >100 0.030181440433563852 3.1862314696031344 >500 0.0023249469965120457 0.8553097761786425 >1k 0.0010074770318216288 0.9003910220453581 >5k 1.5499646643409674E-4 0.7235914536993334 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8986 0.39417086820235103 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7434 0.3260923919670907 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4625 0.20287561378097857 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.386499757426564E-5 2 4.386499757426564E-5 0.0 0.0 0.0 4.386499757426564E-5 3 4.386499757426564E-5 0.0 0.0 0.0 4.386499757426564E-5 4 4.386499757426564E-5 0.0 0.0 0.0 4.386499757426564E-5 5 4.386499757426564E-5 0.0 0.0 0.0 8.772999514853127E-5 6 4.386499757426564E-5 0.0 0.0 0.0 8.772999514853127E-5 7 4.386499757426564E-5 0.0 0.0 0.0 8.772999514853127E-5 8 4.386499757426564E-5 0.0 0.0 0.0 8.772999514853127E-5 9 4.386499757426564E-5 0.0 0.0 0.0 8.772999514853127E-5 10-11 4.386499757426564E-5 0.0 0.0 2.193249878713282E-5 1.0966249393566409E-4 12-13 4.386499757426564E-5 0.0 0.0 4.386499757426564E-5 3.509199805941251E-4 14-15 4.386499757426564E-5 0.0 0.0 4.386499757426564E-5 5.044474721040547E-4 16-17 4.386499757426564E-5 0.0 0.0 8.772999514853127E-5 6.360424648268517E-4 18-19 4.386499757426564E-5 0.0 0.0 1.0966249393566409E-4 6.579749636139845E-4 20-21 4.386499757426564E-5 0.0 0.0 1.9739248908419535E-4 7.23772459975383E-4 22-23 4.386499757426564E-5 0.0 0.0 2.8512248423272663E-4 7.457049587625158E-4 24-25 8.772999514853127E-5 0.0 0.0 4.82514973316922E-4 7.457049587625158E-4 26-27 8.772999514853127E-5 0.0 0.0 9.65029946633844E-4 8.772999514853126E-4 28-29 1.7545999029706255E-4 0.0 0.0 0.003224077321708524 8.772999514853126E-4 30-31 1.7545999029706255E-4 0.0 0.0 0.010549531916610885 8.772999514853126E-4 32-33 1.7545999029706255E-4 0.0 0.0 0.025968078563965255 8.772999514853126E-4 34-35 1.9739248908419535E-4 0.0 0.0 0.04937005476983597 9.211649490595784E-4 36-37 2.1932498787132816E-4 0.0 0.0 0.08919947256726918 9.211649490595784E-4 38-39 2.631899854455938E-4 0.0 0.0 0.14900939675978037 9.211649490595784E-4 40-41 2.631899854455938E-4 0.0 0.0 0.2122627232618714 9.211649490595784E-4 42-43 2.631899854455938E-4 0.0 0.0 0.2725332299289124 9.211649490595784E-4 44-45 2.631899854455938E-4 0.0 0.0 0.3484635407299662 9.430974478467112E-4 46-47 2.8512248423272663E-4 0.0 0.0 0.4373778908130026 9.65029946633844E-4 48-49 3.0705498301985943E-4 0.0 0.0 0.5306129431571043 0.001096624939356641 50-51 3.0705498301985943E-4 0.0 0.0 0.6294627151907118 0.0011843549345051721 52-53 3.0705498301985943E-4 0.0 0.0 0.721623075094244 0.001315949927227969 54-55 3.0705498301985943E-4 0.0 0.0 0.821701067059931 0.0013817474235893676 56-57 3.509199805941251E-4 0.0 0.0 0.9414963754352504 0.0014256124211636332 58-59 3.509199805941251E-4 0.0 0.0 1.069077720880002 0.0014694774187378988 60-61 3.509199805941251E-4 0.0 0.0 1.2007604435979475 0.0014914099175250316 62-63 3.509199805941251E-4 0.0 0.0 1.3310175538947293 0.0015352749150992972 64-65 3.509199805941251E-4 0.0 0.0 1.47000379870879 0.0015791399126735628 66-67 3.947849781683907E-4 0.0 0.0 1.623750615206591 0.0015791399126735628 68-69 3.947849781683907E-4 0.0 0.0 1.7932449658335532 0.0015791399126735628 70-71 3.947849781683907E-4 0.0 0.0 1.9686172261354673 0.0015791399126735628 72-73 4.167174769555235E-4 0.0 0.0 2.1458537488342877 0.0016888024066092269 74-75 4.82514973316922E-4 0.0 0.0 2.327060053813579 0.0017545999029706253 76-77 5.263799708911876E-4 0.0 0.0 2.5227856729899525 0.001776532401757758 78-79 5.263799708911876E-4 0.0 0.0 2.736737198658433 0.001820397399332024 80-81 6.579749636139845E-4 0.0 0.0 2.962291016185307 0.0018423298981191567 82-83 6.579749636139845E-4 0.0 0.0 3.197166145696712 0.0019081273944805551 84-85 7.018399611882502E-4 0.0 0.0 3.4292777803609384 0.001930059893267688 86-87 7.895699563367814E-4 0.0 0.0 3.6769176241664554 0.0019958573896290866 88-89 7.895699563367814E-4 0.0 0.0 3.938089819723633 0.002061654885990485 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4315 0.0 56.895523 1 GTATCAA 7835 0.0 42.002586 1 ATCAACG 9795 0.0 33.33546 3 TCAACGC 9865 0.0 33.1471 4 TATCAAC 10070 0.0 32.5195 2 CAACGCA 10100 0.0 32.328793 5 AACGCAG 10490 0.0 31.254555 6 GTGGTAT 1980 0.0 29.912472 1 CGCAGAG 11905 0.0 27.253744 8 ACGCAGA 11980 0.0 27.241735 7 TGGTATC 1885 0.0 27.231188 2 GCAGAGT 13090 0.0 24.24218 9 TACATGG 7030 0.0 24.06849 2 GTACATG 7455 0.0 23.449265 1 ACATGGG 7355 0.0 22.746477 3 GAGTACT 8535 0.0 22.401556 12-13 CAGAGTA 12200 0.0 22.349747 10-11 AGAGTAC 11570 0.0 21.575453 10-11 GTACTTT 8890 0.0 21.37436 14-15 CATGGGG 4800 0.0 20.199615 4 >>END_MODULE